RLG00000034459

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007736
Physical Location & Seq
Reverse (-)
43033014 .. 43039159
6146 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034459

Sequence Viewer

Length: 1056 bp
ATGGCTCTGGTACCCTCATCAGCACCAGTGAACGAGAGCCCAGTGATCACCGAGGTTGACATGGGCGGCGACGACTCCACACCCACCGTCCGAGCCACCGTCGTCCAAGCCTCCACCGTCTTCTACGACACTCCAGCCACTCTAGATAAGGCTGAGAGATTGTTGGCTGAAGCAGCTGGGTATGGATCGCAGCTGGTTGTGTTTCCAGAAGCATTTGTGGGAGGGTATCCGCGCGGCTCCAATTTCGGGGTTGCGATTGGGAACAGAACTGCCAAGGGGAAAGAAGAGTTCCGAAAGTATCATGCCGCAGCCATTGATGTGCCTGGTCCTGAAGTTGACCGGTTGGCATCAATGGCTGGAAAATATAAGGTATTCTTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACACTTTATTGTACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCATCGAAAAATAATGCCAACAGCATTAGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCCTGTGTTTGATACTCCAATTGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCTCTTTTAAGGACAGCAATGTATGCTAAAGGCATTGAAATATATTGTGCTCCTACAGCTGATTCTAGGGATCTATGGCAAGCGTCAATGACCCATATTGCTTTAGAAGGTGGGTGCTTTGTCTTATCAGCTAATCAATTCTGTCGAAGGAAAGACTATCCCCCTCCACCAGAGTATATATTCTCCGGCACAGAAGAGGAACTTACTCCTGATTCTGTTGTCTGTGCTGGAGGCAGTGTCATCATATCACCGTCAGGGACTATACTTGCTGGGCCCAATTATGACGGGGAGGCACTCATCTCAGCAGATCTTGATATTGGAGATATAGCAAGGGCGAAGTTTGATTTCGATGTGGTAGGGCACTATGCACGGCCTGAAGTGCTTAGCTTGATTGTGAGGGACCATCCAGCAAATCCGGTTACTTTTACATCAGCCTCAGCGAAGACAGAAGTCTCAGACAAATCATAA

Protein Analysis

352

Amino Acids

37.7

Weight (kDa)

4.96

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 31 - 309 2.6e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 10
AccB7I CCANNNNNTGG 1 cut(s) 573
AccI GTMKAC 1 cut(s) 407
AccII CGCG 2 cut(s) 232, 234
AciI CCGC 4 cut(s) 66, 230, 234, 306
AclWI GGATC 4 cut(s) 193, 513, 526, 666
AcuI CTGAAG 3 cut(s) 189, 351, 984
AfaI GTAC 2 cut(s) 12, 421
AfiI CCNNNNNNNGG 2 cut(s) 246, 573
AgeI ACCGGT 1 cut(s) 339
AgsI TTSAA 1 cut(s) 626
AjnI CCWGG 2 cut(s) 322, 453
AluBI AGCT 5 cut(s) 176, 193, 647, 719, 975
AluI AGCT 5 cut(s) 176, 193, 647, 719, 975
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 1045
AlwI GGATC 4 cut(s) 193, 513, 526, 666
AoxI GGCC 2 cut(s) 860, 959
ApaI GGGCCC 1 cut(s) 864
ApeKI GCWGC 4 cut(s) 173, 190, 308, 563
AsiGI ACCGGT 1 cut(s) 339
Asp718I GGTACC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 4 cut(s) 326, 860, 861, 988
AsuHPI GGTGA 2 cut(s) 40, 828
AvaII GGWCC 2 cut(s) 326, 988
BaeGI GKGCMC 2 cut(s) 864, 951
BamHI GGATCC 1 cut(s) 518
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 2 cut(s) 41, 864
BbsI GAAGAC 2 cut(s) 112, 1037
Bbv12I GWGCWC 1 cut(s) 640
BbvCI CCTCAGC 1 cut(s) 1024
BbvI GCAGC 4 cut(s) 185, 202, 320, 550
BccI CCATC 2 cut(s) 509, 999
BceAI ACGGC 1 cut(s) 974
BciT130I CCWGG 2 cut(s) 324, 455
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 1045
BfaI CTAG 2 cut(s) 143, 654
BfmI CTRYAG 1 cut(s) 642
BfuI GTATCC 1 cut(s) 237
BglII AGATCT 1 cut(s) 895
BisI GCNGC 7 cut(s) 67, 174, 191, 235, 306, 309, 564
BlpI GCTNAGC 1 cut(s) 971
BlsI GCNGC 7 cut(s) 68, 175, 192, 236, 307, 310, 565
Bme1390I CCNGG 2 cut(s) 324, 455
Bme18I GGWCC 2 cut(s) 326, 988
BmgT120I GGNCC 4 cut(s) 326, 860, 861, 988
BmiI GGNNCC 5 cut(s) 12, 238, 520, 862, 989
BmrFI CCNGG 2 cut(s) 324, 455
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 3 cut(s) 356, 472, 576
BmuI ACTGGG 1 cut(s) 35
BoxI GACNNNNGTC 1 cut(s) 1037
BpiI GAAGAC 2 cut(s) 112, 1037
BpmI CTGGAG 2 cut(s) 117, 837
Bpu10I CCTNAGC 1 cut(s) 1024
Bpu1102I GCTNAGC 1 cut(s) 971
BpuEI CTTGAG 1 cut(s) 426
BsaJI CCNNGG 3 cut(s) 51, 273, 454
BsaWI WCCGGW 2 cut(s) 339, 1003
BsaXI ACNNNNNCTCC 2 cut(s) 810, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 573
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 2 cut(s) 26, 41
Bse3DI GCAATG 1 cut(s) 612
BseBI CCWGG 2 cut(s) 324, 455
BseDI CCNNGG 3 cut(s) 51, 273, 454
BseGI GGATG 1 cut(s) 991
BseLI CCNNNNNNNGG 2 cut(s) 246, 573
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 4 cut(s) 144, 903, 1038, 1056
BseNI ACTGG 2 cut(s) 26, 41
BseSI GKGCMC 2 cut(s) 864, 951
BseXI GCAGC 4 cut(s) 185, 202, 320, 550
BseYI CCCAGC 2 cut(s) 176, 857
Bsh1236I CGCG 2 cut(s) 232, 234
BshFI GGCC 2 cut(s) 862, 961
BshNI GGYRCC 1 cut(s) 10
BshTI ACCGGT 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 3 cut(s) 340, 774, 1004
BslFI GGGAC 2 cut(s) 859, 1001
BslI CCNNNNNNNGG 2 cut(s) 246, 573
BsmAI GTCTC 1 cut(s) 1045
BsmFI GGGAC 2 cut(s) 859, 1001
BsnI GGCC 2 cut(s) 862, 961
Bsp120I GGGCCC 1 cut(s) 860
Bsp1286I GDGCHC 4 cut(s) 41, 640, 864, 951
Bsp143I GATC 5 cut(s) 45, 185, 518, 658, 895
Bsp1720I GCTNAGC 1 cut(s) 971
BspACI CCGC 4 cut(s) 66, 230, 234, 306
BspANI GGCC 2 cut(s) 862, 961
BspCNI CTCAG 4 cut(s) 145, 902, 1037, 1055
BspFNI CGCG 2 cut(s) 232, 234
BspLI GGNNCC 5 cut(s) 12, 238, 520, 862, 989
BspPI GGATC 4 cut(s) 193, 513, 526, 666
BspT107I GGYRCC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 2 cut(s) 26, 41
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 3 cut(s) 51, 273, 454
BssMI GATC 5 cut(s) 45, 185, 518, 658, 895
BssNAI GTATAC 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 324, 455
Bst4CI ACNGT 5 cut(s) 88, 100, 118, 424, 840
Bst6I CTCTTC 2 cut(s) 279, 777
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 669
BstDEI CTNAG 5 cut(s) 153, 889, 971, 1024, 1042
BstF5I GGATG 1 cut(s) 991
BstFNI CGCG 2 cut(s) 232, 234
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 5 cut(s) 48, 188, 521, 661, 898
BstMAI GTCTC 1 cut(s) 1045
BstMBI GATC 5 cut(s) 45, 185, 518, 658, 895
BstMWI GCNNNNNNNGC 5 cut(s) 173, 353, 611, 644, 967
BstNI CCWGG 2 cut(s) 324, 455
BstPAI GACNNNNGTC 1 cut(s) 1037
BstSCI CCNGG 2 cut(s) 322, 453
BstSFI CTRYAG 1 cut(s) 642
BstSLI GKGCMC 2 cut(s) 864, 951
BstUI CGCG 2 cut(s) 232, 234
BstV1I GCAGC 4 cut(s) 185, 202, 320, 550
BstV2I GAAGAC 2 cut(s) 112, 1037
BstX2I RGATCY 3 cut(s) 518, 658, 895
BstYI RGATCY 3 cut(s) 518, 658, 895
BstZ17I GTATAC 1 cut(s) 408
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 2 cut(s) 862, 961
BtsCI GGATG 1 cut(s) 991
BtsI GCAGTG 1 cut(s) 829
BtsIMutI CAGTG 3 cut(s) 33, 48, 829
Cac8I GCNNGC 1 cut(s) 669
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 339
Cfr13I GGNCC 4 cut(s) 326, 860, 861, 988
CseI GACGC 1 cut(s) 660
Csp6I GTAC 2 cut(s) 11, 420
CspAI ACCGGT 1 cut(s) 339
CviAII CATG 2 cut(s) 61, 302
CviQI GTAC 2 cut(s) 11, 420
DdeI CTNAG 5 cut(s) 153, 889, 971, 1024, 1042
DpnI GATC 5 cut(s) 47, 187, 520, 660, 897
DpnII GATC 5 cut(s) 45, 185, 518, 658, 895
Eam1104I CTCTTC 2 cut(s) 279, 777
EarI CTCTTC 2 cut(s) 279, 777
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 2 cut(s) 41, 864
Eco47I GGWCC 2 cut(s) 326, 988
Eco57I CTGAAG 3 cut(s) 189, 351, 984
EcoRII CCWGG 2 cut(s) 322, 453
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 2 cut(s) 41, 864
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 64, 305
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 774, 806
FaqI GGGAC 2 cut(s) 859, 1001
FatI CATG 2 cut(s) 60, 301
FbaI TGATCA 1 cut(s) 45
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 7 cut(s) 67, 174, 191, 235, 306, 309, 564
FokI GGATG 1 cut(s) 978
FriOI GRGCYC 2 cut(s) 41, 864
Fsp4HI GCNGC 7 cut(s) 67, 174, 191, 235, 306, 309, 564
FspBI CTAG 2 cut(s) 143, 654
GlaI GCGC 1 cut(s) 233
GluI GCNGC 7 cut(s) 67, 174, 191, 235, 306, 309, 564
GsaI CCCAGC 2 cut(s) 180, 861
GsuI CTGGAG 2 cut(s) 117, 837
HaeIII GGCC 2 cut(s) 862, 961
HapII CCGG 3 cut(s) 340, 774, 1004
HgaI GACGC 1 cut(s) 660
HhaI GCGC 1 cut(s) 234
Hin1II CATG 2 cut(s) 64, 305
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 2 cut(s) 58, 337
HindII GTYRAC 2 cut(s) 58, 337
HinfI GANTC 5 cut(s) 74, 437, 495, 650, 800
HpaII CCGG 3 cut(s) 340, 774, 1004
HphI GGTGA 2 cut(s) 40, 828
Hpy166II GTNNAC 4 cut(s) 31, 58, 337, 408
Hpy188I TCNGA 3 cut(s) 92, 293, 1045
Hpy188III TCNNGA 5 cut(s) 143, 206, 329, 797, 899
Hpy8I GTNNAC 4 cut(s) 31, 58, 337, 408
Hpy99I CGWCG 2 cut(s) 74, 104
HpyAV CCTTC 3 cut(s) 395, 689, 729
HpyCH4III ACNGT 5 cut(s) 88, 100, 118, 424, 840
HpyCH4V TGCA 1 cut(s) 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 353, 611, 644, 967
HpyF3I CTNAG 5 cut(s) 153, 889, 971, 1024, 1042
Hsp92II CATG 2 cut(s) 64, 305
HspAI GCGC 1 cut(s) 232
KpnI GGTACC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 5 cut(s) 45, 185, 518, 658, 895
LmnI GCTCC 2 cut(s) 242, 643
Lsp1109I GCAGC 4 cut(s) 185, 202, 320, 550
LweI GCATC 3 cut(s) 356, 472, 576
MaeI CTAG 2 cut(s) 143, 654
MaeIII GTNAC 1 cut(s) 1006
MalI GATC 5 cut(s) 47, 187, 520, 660, 897
MboI GATC 5 cut(s) 45, 185, 518, 658, 895
MboII GAAGA 4 cut(s) 112, 296, 794, 1042
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 3 cut(s) 518, 658, 895
MhlI GDGCHC 4 cut(s) 41, 640, 864, 951
MluCI AATT 5 cut(s) 241, 525, 546, 725, 865
MlyI GAGTC 1 cut(s) 68
MseI TTAA 1 cut(s) 596
MslI CAYNNNNRTG 1 cut(s) 317
MspA1I CMGCKG 3 cut(s) 176, 193, 647
MspI CCGG 3 cut(s) 340, 774, 1004
MspR9I CCNGG 2 cut(s) 324, 455
MunI CAATTG 1 cut(s) 546
MvaI CCWGG 2 cut(s) 324, 455
MvnI CGCG 2 cut(s) 232, 234
MwoI GCNNNNNNNGC 5 cut(s) 173, 353, 611, 644, 967
NdeII GATC 5 cut(s) 45, 185, 518, 658, 895
NlaIII CATG 2 cut(s) 64, 305
NlaIV GGNNCC 5 cut(s) 12, 238, 520, 862, 989
PfeI GAWTC 4 cut(s) 437, 495, 650, 800
PflMI CCANNNNNTGG 1 cut(s) 573
PinAI ACCGGT 1 cut(s) 339
PkrI GCNGC 7 cut(s) 68, 175, 192, 236, 307, 310, 565
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PshAI GACNNNNGTC 1 cut(s) 1037
Psp6I CCWGG 2 cut(s) 322, 453
PspFI CCCAGC 2 cut(s) 176, 857
PspGI CCWGG 2 cut(s) 322, 453
PspN4I GGNNCC 5 cut(s) 12, 238, 520, 862, 989
PspOMI GGGCCC 1 cut(s) 860
PspPI GGNCC 4 cut(s) 326, 860, 861, 988
PsuI RGATCY 3 cut(s) 518, 658, 895
PvuII CAGCTG 3 cut(s) 176, 193, 647
RsaI GTAC 2 cut(s) 12, 421
RsaNI GTAC 2 cut(s) 11, 420
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 596
SatI GCNGC 7 cut(s) 67, 174, 191, 235, 306, 309, 564
Sau3AI GATC 5 cut(s) 45, 185, 518, 658, 895
Sau96I GGNCC 4 cut(s) 326, 860, 861, 988
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 324, 455
SduI GDGCHC 4 cut(s) 41, 640, 864, 951
SfaNI GCATC 3 cut(s) 356, 472, 576
SfcI CTRYAG 1 cut(s) 642
SinI GGWCC 2 cut(s) 326, 988
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 5 cut(s) 241, 525, 546, 725, 865
SsiI CCGC 4 cut(s) 66, 230, 234, 306
SspMI CTAG 2 cut(s) 143, 654
StyD4I CCNGG 2 cut(s) 322, 453
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 5 cut(s) 88, 100, 118, 424, 840
TaqI TCGA 3 cut(s) 466, 733, 936
TasI AATT 5 cut(s) 241, 525, 546, 725, 865
TatI WGTACW 1 cut(s) 419
TauI GCSGC 3 cut(s) 69, 237, 308
TfiI GAWTC 4 cut(s) 437, 495, 650, 800
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TscAI CASTG 3 cut(s) 33, 48, 829
TseI GCWGC 4 cut(s) 173, 190, 308, 563
TspRI CASTG 3 cut(s) 33, 48, 829
Van91I CCANNNNNTGG 1 cut(s) 573
VpaK11BI GGWCC 2 cut(s) 326, 988
XbaI TCTAGA 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 2 cut(s) 143, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.