Rh5BG321600

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007736
Physical Location & Seq
Reverse (-)
43094052 .. 43100585
6534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG321600.1

Sequence Viewer

Length: 783 bp
ATGAAGAAGCGGCATGAATATGTAGATTCACTATTTCTATTAATAAATGCAGGTCCTGAAGTTGACCGATTGGCATCAATGGCTGGAAAATATAAGGTATTCTTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACACTTTATTGTACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCACCGAAAAATAATGCCAACAGCATTAGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCCTGTGTTTGATACTCCAATAGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCACTTTTAAGGACAGCAATGTATGCTAAAGGCATTGAAATATATTGTGCTCCTACAGCTGATTCTAGGGATCTATGGCAAGCGTCAATGACCCATATTGCTTTAGAAGGTGGGTGCTTTGTCTTATCAGCTAACCAATTCTGTCGAAGGAAAGACTATCCCCCTCCACCAGAGTATATATTTTCAGGTACGGAAGAGGAACTTACTCCTGATTCTGTTGTCTGTGCTGGAGGCAGTGTCATCATATCACCATCAGGGACTATACTTGCTGGGCCCAATTATGACGGGGAGGCACTCATCTCAGCAGATCTTGATATTGGAGATATAGCAAGGGCGAAGTTTGATTTCGATGTGGTAGGACACTATGCACGGCCTGAAGTGCTTAGCTTGATTGTGAGGGACCATCCAGCAAATCCGGTTACTTTTACATCAGCCTCAGCGAAGACAGAAGTCTCAGACAAATCATAA

Protein Analysis

260

Amino Acids

28.46

Weight (kDa)

5.29

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 17 - 218 3.2e-47 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 41
AccB7I CCANNNNNTGG 1 cut(s) 300
AccI GTMKAC 1 cut(s) 134
AciI CCGC 1 cut(s) 10
AclWI GGATC 3 cut(s) 240, 253, 393
AcuI CTGAAG 2 cut(s) 78, 711
AfaI GTAC 2 cut(s) 148, 505
AfiI CCNNNNNNNGG 1 cut(s) 300
AgsI TTSAA 1 cut(s) 353
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 3 cut(s) 374, 446, 702
AluI AGCT 3 cut(s) 374, 446, 702
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 1 cut(s) 772
AlwI GGATC 3 cut(s) 240, 253, 393
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 2 cut(s) 587, 686
ApaI GGGCCC 1 cut(s) 591
ApeKI GCWGC 1 cut(s) 290
AseI ATTAAT 1 cut(s) 41
AspS9I GGNCC 4 cut(s) 53, 587, 588, 715
AsuHPI GGTGA 1 cut(s) 555
AvaII GGWCC 2 cut(s) 53, 715
BaeGI GKGCMC 1 cut(s) 591
BamHI GGATCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 591
BbsI GAAGAC 1 cut(s) 764
Bbv12I GWGCWC 1 cut(s) 367
BbvCI CCTCAGC 1 cut(s) 751
BbvI GCAGC 1 cut(s) 277
BccI CCATC 3 cut(s) 236, 574, 726
BceAI ACGGC 1 cut(s) 701
BciT130I CCWGG 1 cut(s) 182
BcoDI GTCTC 1 cut(s) 772
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 369
BfuAI ACCTGC 1 cut(s) 41
BglII AGATCT 1 cut(s) 622
BisI GCNGC 2 cut(s) 11, 291
BlpI GCTNAGC 1 cut(s) 698
BlsI GCNGC 2 cut(s) 12, 292
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 2 cut(s) 53, 715
BmgT120I GGNCC 4 cut(s) 53, 587, 588, 715
BmiI GGNNCC 3 cut(s) 247, 589, 716
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 2 cut(s) 83, 303
BoxI GACNNNNGTC 1 cut(s) 764
BpiI GAAGAC 1 cut(s) 764
BpmI CTGGAG 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 751
Bpu1102I GCTNAGC 1 cut(s) 698
BpuEI CTTGAG 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 1 cut(s) 730
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 339
Bse8I GATNNNNATC 1 cut(s) 73
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 718
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 300
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 3 cut(s) 630, 765, 783
BseSI GKGCMC 1 cut(s) 591
BseXI GCAGC 1 cut(s) 277
BseYI CCCAGC 1 cut(s) 584
BshFI GGCC 2 cut(s) 589, 688
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 2 cut(s) 586, 728
BslI CCNNNNNNNGG 1 cut(s) 300
BsmAI GTCTC 1 cut(s) 772
BsmFI GGGAC 2 cut(s) 586, 728
BsnI GGCC 2 cut(s) 589, 688
Bsp120I GGGCCC 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 367, 591
Bsp143I GATC 3 cut(s) 245, 385, 622
Bsp1720I GCTNAGC 1 cut(s) 698
BspACI CCGC 1 cut(s) 10
BspANI GGCC 2 cut(s) 589, 688
BspCNI CTCAG 3 cut(s) 629, 764, 782
BspLI GGNNCC 3 cut(s) 247, 589, 716
BspMI ACCTGC 1 cut(s) 41
BspPI GGATC 3 cut(s) 240, 253, 393
BsrDI GCAATG 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 3 cut(s) 245, 385, 622
BssNAI GTATAC 1 cut(s) 135
Bst1107I GTATAC 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 504
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 4 cut(s) 616, 698, 751, 769
BstF5I GGATG 1 cut(s) 718
BstKTI GATC 3 cut(s) 248, 388, 625
BstMAI GTCTC 1 cut(s) 772
BstMBI GATC 3 cut(s) 245, 385, 622
BstMWI GCNNNNNNNGC 4 cut(s) 80, 338, 371, 694
BstNI CCWGG 1 cut(s) 182
BstPAI GACNNNNGTC 1 cut(s) 764
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 591
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 764
BstX2I RGATCY 3 cut(s) 245, 385, 622
BstYI RGATCY 3 cut(s) 245, 385, 622
BstZ17I GTATAC 1 cut(s) 135
BsuRI GGCC 2 cut(s) 589, 688
BtsCI GGATG 1 cut(s) 718
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 1 cut(s) 556
BveI ACCTGC 1 cut(s) 41
Cac8I GCNNGC 1 cut(s) 396
CaiI CAGNNNCTG 1 cut(s) 56
Cfr13I GGNCC 4 cut(s) 53, 587, 588, 715
CseI GACGC 1 cut(s) 387
Csp6I GTAC 2 cut(s) 147, 504
CviAII CATG 1 cut(s) 14
CviJI RGCY 7 cut(s) 83, 374, 446, 589, 688, 702, 749
CviKI_1 RGCY 7 cut(s) 83, 374, 446, 589, 688, 702, 749
CviQI GTAC 2 cut(s) 147, 504
DdeI CTNAG 4 cut(s) 616, 698, 751, 769
DpnI GATC 3 cut(s) 247, 387, 624
DpnII GATC 3 cut(s) 245, 385, 622
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 591
Eco47I GGWCC 2 cut(s) 53, 715
Eco57I CTGAAG 2 cut(s) 78, 711
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 591
FaeI CATG 1 cut(s) 17
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 501, 533
FaqI GGGAC 2 cut(s) 586, 728
FatI CATG 1 cut(s) 13
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 2 cut(s) 11, 291
FokI GGATG 1 cut(s) 705
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 2 cut(s) 11, 291
FspBI CTAG 1 cut(s) 381
GluI GCNGC 2 cut(s) 11, 291
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 2 cut(s) 589, 688
HapII CCGG 1 cut(s) 731
HgaI GACGC 1 cut(s) 387
Hin1II CATG 1 cut(s) 17
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 5 cut(s) 26, 164, 222, 377, 527
HpaII CCGG 1 cut(s) 731
HphI GGTGA 1 cut(s) 555
Hpy166II GTNNAC 2 cut(s) 64, 135
Hpy188I TCNGA 1 cut(s) 772
Hpy188III TCNNGA 3 cut(s) 56, 524, 626
Hpy8I GTNNAC 2 cut(s) 64, 135
HpyAV CCTTC 3 cut(s) 122, 416, 456
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 2 cut(s) 50, 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 338, 371, 694
HpyF3I CTNAG 4 cut(s) 616, 698, 751, 769
Hsp92II CATG 1 cut(s) 17
Kzo9I GATC 3 cut(s) 245, 385, 622
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 2 cut(s) 83, 303
MaeI CTAG 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 733
MalI GATC 3 cut(s) 247, 387, 624
MboI GATC 3 cut(s) 245, 385, 622
MboII GAAGA 3 cut(s) 16, 521, 769
MflI RGATCY 3 cut(s) 245, 385, 622
MhlI GDGCHC 2 cut(s) 367, 591
MluCI AATT 3 cut(s) 252, 452, 592
MnlI CCTC 6 cut(s) 489, 505, 539, 598, 705, 760
MseI TTAA 2 cut(s) 41, 323
MslI CAYNNNNRTG 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 80, 338, 371, 694
NdeII GATC 3 cut(s) 245, 385, 622
NlaIII CATG 1 cut(s) 17
NlaIV GGNNCC 3 cut(s) 247, 589, 716
PfeI GAWTC 5 cut(s) 26, 164, 222, 377, 527
PflMI CCANNNNNTGG 1 cut(s) 300
PkrI GCNGC 2 cut(s) 12, 292
PpuMI RGGWCCY 1 cut(s) 53
PshAI GACNNNNGTC 1 cut(s) 764
PshBI ATTAAT 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 247, 589, 716
PspOMI GGGCCC 1 cut(s) 587
PspPI GGNCC 4 cut(s) 53, 587, 588, 715
PspPPI RGGWCCY 1 cut(s) 53
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 3 cut(s) 245, 385, 622
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 2 cut(s) 148, 505
RsaNI GTAC 2 cut(s) 147, 504
RseI CAYNNNNRTG 1 cut(s) 18
SaqAI TTAA 2 cut(s) 41, 323
SatI GCNGC 2 cut(s) 11, 291
Sau3AI GATC 3 cut(s) 245, 385, 622
Sau96I GGNCC 4 cut(s) 53, 587, 588, 715
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 2 cut(s) 367, 591
SfaNI GCATC 2 cut(s) 83, 303
SfcI CTRYAG 1 cut(s) 369
SinI GGWCC 2 cut(s) 53, 715
SmiMI CAYNNNNRTG 1 cut(s) 18
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
Sse9I AATT 3 cut(s) 252, 452, 592
SsiI CCGC 1 cut(s) 10
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 151
TaqI TCGA 2 cut(s) 460, 663
TaqII GACCGA 1 cut(s) 81
TasI AATT 3 cut(s) 252, 452, 592
TatI WGTACW 1 cut(s) 146
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 5 cut(s) 26, 164, 222, 377, 527
Tru1I TTAA 2 cut(s) 41, 323
Tru9I TTAA 2 cut(s) 41, 323
TscAI CASTG 1 cut(s) 556
TseI GCWGC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 17, 30
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 1 cut(s) 556
Van91I CCANNNNNTGG 1 cut(s) 300
VpaK11BI GGWCC 2 cut(s) 53, 715
VspI ATTAAT 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 134
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.