AT3G44310

nitrilase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007736
Physical Location & Seq
Forward (+)
15986624 .. 15989505
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44310.2

Sequence Viewer

Length: 1041 bp
ATGTCTAGTACTAAAGATATGTCAACTGTCCAAAACGCAACTCCTTTTAACGGCGTTGCCCCATCCACCACCGTGCGAGTTACAATCGTCCAATCCTCCACCGTCTATAACGATACTCCTGCCACTATAGACAAAGCGGAGAAGTATATTGTGGAGGCGGCAAGCAAGGGAGCAGAGCTAGTGTTGTTCCCGGAGGGGTTTATCGGTGGCTATCCTCGAGGTTTTAGGTTCGGTTTAGCGGTTGGCGTTCATAACGAAGAAGGGCGTGATGAGTTTCGGAAGTACCATGCTTCTGCTATTCATGTTCCTGGCCCTGAAGTAGCAAGATTGGCTGACGTGGCTAGGAAAAACCATGTGTACTTGGTAATGGGAGCCATAGAGAAGGAAGGGTATACCCTCTATTGCACAGTTCTTTTCTTTAGTCCACAGGGTCAGTTCTTGGGCAAGCACCGTAAACTCATGCCCACAAGTTTGGAACGTTGCATTTGGGGCCAAGGGGACGGATCAACCATCCCCGTTTACGACACTCCCATTGGAAAACTCGGTGCTGCTATTTGCTGGGAGAATAGGATGCCCCTCTACAGAACTGCATTGTACGCCAAAGGCATTGAGCTTTATTGTGCACCTACTGCTGATGGTTCGAAAGAATGGCAATCGTCGATGCTTCACATTGCGATCGAAGGTGGATGTTTCGTCTTGTCGGCTTGCCAATTCTGCCAGCGTAAACATTTCCCTGATCATCCTGACTACTTGTTTACCGATTGGTACGACGACAAAGAACATGATTCTATTGTCTCCCAAGGTGGAAGTGTCATTATTTCACCTTTGGGACAAGTTCTCGCCGGACCAAACTTTGAATCAGAGGGTCTCGTCACAGCTGATATTGATCTTGGTGATATAGCAAGAGCCAAGTTATACTTCGATTCGGTTGGACATTACTCGAGACCAGATGTTTTACACTTGACCGTAAATGAGCACCCGAGGAAATCGGTTACATTCGTGACGAAGGTGGAGAAAGCTGAGGATGACTCAAACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000257 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009636 GO:0009683 GO:0009684 GO:0009850 GO:0009851 GO:0009970 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0010817 GO:0016020 GO:0016053 GO:0016143 GO:0016145 GO:0016787 GO:0016810 GO:0016815 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0018822 GO:0019438 GO:0019499 GO:0019748 GO:0019752 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0030054 GO:0031667 GO:0031668 GO:0031669 GO:0032787 GO:0033554 GO:0034641 GO:0034754 GO:0042221 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044248 GO:0044249 GO:0044271 GO:0044273 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046686 GO:0047427 GO:0047558 GO:0048046 GO:0050896 GO:0051410 GO:0051716 GO:0055044 GO:0065007 GO:0065008 GO:0071496 GO:0071704 GO:0071944 GO:0072330 GO:0080061 GO:0080109 GO:0098754 GO:1901135 GO:1901136 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901698
KEGG Pathways
Metabolic & Signaling
ko00380 ko00460 ko00627 ko00643 ko00910 ko01100 ko01110 ko01120 map00380 map00460 map00627 map00643 map00910 map01100 map01110 map01120
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

346

Amino Acids

38.15

Weight (kDa)

5.85

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 1 - 182 8.1e-45 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 216
AccI GTMKAC 1 cut(s) 392
AciI CCGC 3 cut(s) 137, 158, 239
AclI AACGTT 1 cut(s) 478
AclWI GGATC 1 cut(s) 511
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 5 cut(s) 10, 284, 359, 596, 767
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 1 cut(s) 857
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 1 cut(s) 307
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 4 cut(s) 178, 613, 878, 1019
AluI AGCT 4 cut(s) 178, 613, 878, 1019
Alw21I GWGCWC 2 cut(s) 625, 978
Alw26I GTCTC 3 cut(s) 799, 872, 937
Alw44I GTGCAC 1 cut(s) 621
AlwI GGATC 1 cut(s) 511
Ama87I CYCGRG 3 cut(s) 216, 940, 979
AoxI GGCC 2 cut(s) 310, 490
ApaLI GTGCAC 1 cut(s) 621
ApeKI GCWGC 1 cut(s) 548
AspS9I GGNCC 3 cut(s) 311, 490, 845
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 813, 905
AsuII TTCGAA 1 cut(s) 641
AvaI CYCGRG 3 cut(s) 216, 940, 979
AvaII GGWCC 1 cut(s) 845
BaeGI GKGCMC 1 cut(s) 625
Bbv12I GWGCWC 2 cut(s) 625, 978
BbvCI CCTCAGC 1 cut(s) 1020
BbvI GCAGC 1 cut(s) 535
BccI CCATC 3 cut(s) 70, 518, 629
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 3 cut(s) 799, 872, 937
BfaI CTAG 3 cut(s) 6, 179, 342
BfmI CTRYAG 2 cut(s) 126, 580
BisI GCNGC 2 cut(s) 159, 549
BlsI GCNGC 2 cut(s) 160, 550
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 2 cut(s) 191, 309
Bme18I GGWCC 1 cut(s) 845
BmeT110I CYCGRG 3 cut(s) 216, 940, 979
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 3 cut(s) 311, 490, 845
BmiI GGNNCC 2 cut(s) 373, 491
BmrFI CCNGG 2 cut(s) 191, 309
BmsI GCATC 2 cut(s) 561, 651
BplI GAGNNNNNCTC 1 cut(s) 1013
Bpu10I CCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 885
BsaI GGTCTC 2 cut(s) 872, 937
BsaJI CCNNGG 3 cut(s) 493, 799, 980
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse3DI GCAATG 1 cut(s) 669
Bse8I GATNNNNATC 1 cut(s) 885
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 3 cut(s) 493, 799, 980
BseGI GGATG 6 cut(s) 62, 510, 576, 692, 739, 1030
BseJI GATNNNNATC 1 cut(s) 885
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 1011
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 1 cut(s) 535
BseYI CCCAGC 1 cut(s) 558
Bsh1285I CGRYCG 1 cut(s) 678
BshFI GGCC 2 cut(s) 312, 492
BsiEI CGRYCG 1 cut(s) 678
BsiHKAI GWGCWC 2 cut(s) 625, 978
BsiHKCI CYCGRG 3 cut(s) 216, 940, 979
BsiSI CCGG 2 cut(s) 191, 843
BslFI GGGAC 2 cut(s) 512, 843
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 3 cut(s) 799, 872, 937
BsmFI GGGAC 2 cut(s) 512, 843
BsnI GGCC 2 cut(s) 312, 492
Bso31I GGTCTC 2 cut(s) 872, 937
BsoBI CYCGRG 3 cut(s) 216, 940, 979
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 625, 978
Bsp143I GATC 4 cut(s) 503, 675, 736, 886
BspACI CCGC 3 cut(s) 137, 158, 239
BspANI GGCC 2 cut(s) 312, 492
BspCNI CTCAG 1 cut(s) 1012
BspLI GGNNCC 2 cut(s) 373, 491
BspPI GGATC 1 cut(s) 511
BspT104I TTCGAA 1 cut(s) 641
BspTNI GGTCTC 2 cut(s) 872, 937
BsrDI GCAATG 1 cut(s) 669
BssECI CCNNGG 3 cut(s) 493, 799, 980
BssMI GATC 4 cut(s) 503, 675, 736, 886
BssNAI GTATAC 1 cut(s) 393
BssT1I CCWWGG 2 cut(s) 493, 799
Bst1107I GTATAC 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 6 cut(s) 28, 73, 103, 409, 452, 967
BstAPI GCANNNNNTGC 1 cut(s) 629
BstBI TTCGAA 1 cut(s) 641
BstC8I GCNNGC 4 cut(s) 163, 446, 706, 719
BstDEI CTNAG 1 cut(s) 1020
BstF5I GGATG 6 cut(s) 62, 510, 576, 692, 739, 1030
BstKTI GATC 4 cut(s) 506, 678, 739, 889
BstMAI GTCTC 3 cut(s) 799, 872, 937
BstMBI GATC 4 cut(s) 503, 675, 736, 886
BstMCI CGRYCG 1 cut(s) 678
BstMWI GCNNNNNNNGC 6 cut(s) 329, 338, 489, 596, 629, 714
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 2 cut(s) 189, 307
BstSFI CTRYAG 2 cut(s) 126, 580
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 1 cut(s) 535
BstXI CCANNNNNNTGG 1 cut(s) 472
BstZ17I GTATAC 1 cut(s) 393
BsuRI GGCC 2 cut(s) 312, 492
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 6 cut(s) 62, 510, 576, 692, 739, 1030
Cac8I GCNNGC 4 cut(s) 163, 446, 706, 719
Cfr13I GGNCC 3 cut(s) 311, 490, 845
Csp6I GTAC 5 cut(s) 9, 283, 358, 595, 766
CviAII CATG 5 cut(s) 287, 302, 353, 460, 782
CviQI GTAC 5 cut(s) 9, 283, 358, 595, 766
DdeI CTNAG 1 cut(s) 1020
DpnI GATC 4 cut(s) 505, 677, 738, 888
DpnII GATC 4 cut(s) 503, 675, 736, 886
Eco130I CCWWGG 2 cut(s) 493, 799
Eco31I GGTCTC 2 cut(s) 872, 937
Eco47I GGWCC 1 cut(s) 845
Eco57I CTGAAG 1 cut(s) 336
Eco88I CYCGRG 3 cut(s) 216, 940, 979
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 493, 799
ErhI CCWWGG 2 cut(s) 493, 799
FaeI CATG 5 cut(s) 290, 305, 356, 463, 785
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 2 cut(s) 512, 843
FatI CATG 5 cut(s) 286, 301, 352, 459, 781
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 2 cut(s) 159, 549
FokI GGATG 6 cut(s) 49, 497, 583, 699, 726, 1037
Fsp4HI GCNGC 2 cut(s) 159, 549
FspBI CTAG 3 cut(s) 6, 179, 342
GluI GCNGC 2 cut(s) 159, 549
GsaI CCCAGC 1 cut(s) 562
HaeIII GGCC 2 cut(s) 312, 492
HapII CCGG 2 cut(s) 191, 843
Hin1II CATG 5 cut(s) 290, 305, 356, 463, 785
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 4 cut(s) 785, 857, 923, 1028
HpaII CCGG 2 cut(s) 191, 843
HphI GGTGA 2 cut(s) 813, 905
Hpy166II GTNNAC 9 cut(s) 24, 358, 393, 425, 455, 520, 623, 725, 756
Hpy188I TCNGA 2 cut(s) 279, 862
Hpy188III TCNNGA 3 cut(s) 743, 942, 1000
Hpy8I GTNNAC 9 cut(s) 24, 358, 393, 425, 455, 520, 623, 725, 756
Hpy99I CGWCG 2 cut(s) 661, 773
HpyAV CCTTC 5 cut(s) 254, 376, 380, 674, 1000
HpyCH4III ACNGT 6 cut(s) 28, 73, 103, 409, 452, 967
HpyCH4IV ACGT 2 cut(s) 336, 478
HpyCH4V TGCA 4 cut(s) 405, 483, 590, 623
HpyF10VI GCNNNNNNNGC 6 cut(s) 329, 338, 489, 596, 629, 714
HpyF3I CTNAG 1 cut(s) 1020
HpySE526I ACGT 2 cut(s) 336, 478
Hsp92II CATG 5 cut(s) 290, 305, 356, 463, 785
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 4 cut(s) 503, 675, 736, 886
LmnI GCTCC 2 cut(s) 170, 371
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 2 cut(s) 561, 651
MaeI CTAG 3 cut(s) 6, 179, 342
MaeII ACGT 2 cut(s) 336, 478
MaeIII GTNAC 4 cut(s) 79, 871, 991, 1000
MalI GATC 4 cut(s) 505, 677, 738, 888
MboI GATC 4 cut(s) 503, 675, 736, 886
MboII GAAGA 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 625, 978
MluCI AATT 1 cut(s) 710
MlyI GAGTC 1 cut(s) 1022
MmeI TCCRAC 1 cut(s) 910
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 2 cut(s) 191, 843
MspR9I CCNGG 2 cut(s) 191, 309
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 6 cut(s) 329, 338, 489, 596, 629, 714
NciI CCSGG 1 cut(s) 191
NdeII GATC 4 cut(s) 503, 675, 736, 886
NlaIII CATG 5 cut(s) 290, 305, 356, 463, 785
NlaIV GGNNCC 2 cut(s) 373, 491
NmuCI GTSAC 2 cut(s) 871, 1000
NspV TTCGAA 1 cut(s) 641
OliI CACNNNNGTG 1 cut(s) 71
PaeR7I CTCGAG 2 cut(s) 216, 940
PfeI GAWTC 3 cut(s) 785, 857, 923
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 2 cut(s) 160, 550
Ple19I CGATCG 1 cut(s) 678
PleI GAGTC 1 cut(s) 1022
PpsI GAGTC 1 cut(s) 1022
Psp1406I AACGTT 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 307
PspFI CCCAGC 1 cut(s) 558
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 373, 491
PspPI GGNCC 3 cut(s) 311, 490, 845
PspXI VCTCGAGB 1 cut(s) 216
PvuI CGATCG 1 cut(s) 678
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 5 cut(s) 10, 284, 359, 596, 767
RsaNI GTAC 5 cut(s) 9, 283, 358, 595, 766
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 2 cut(s) 159, 549
Sau3AI GATC 4 cut(s) 503, 675, 736, 886
Sau96I GGNCC 3 cut(s) 311, 490, 845
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 2 cut(s) 191, 309
SduI GDGCHC 2 cut(s) 625, 978
SfaNI GCATC 2 cut(s) 561, 651
SfcI CTRYAG 2 cut(s) 126, 580
Sfr274I CTCGAG 2 cut(s) 216, 940
SfuI TTCGAA 1 cut(s) 641
SinI GGWCC 1 cut(s) 845
SlaI CTCGAG 2 cut(s) 216, 940
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 2 cut(s) 216, 940
SmoI CTYRAG 2 cut(s) 216, 940
Sse9I AATT 1 cut(s) 710
SsiI CCGC 3 cut(s) 137, 158, 239
SspMI CTAG 3 cut(s) 6, 179, 342
StyD4I CCNGG 2 cut(s) 189, 307
StyI CCWWGG 2 cut(s) 493, 799
TaaI ACNGT 6 cut(s) 28, 73, 103, 409, 452, 967
TaiI ACGT 2 cut(s) 339, 481
TaqI TCGA 6 cut(s) 217, 641, 659, 678, 921, 941
TasI AATT 1 cut(s) 710
TatI WGTACW 2 cut(s) 8, 357
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 3 cut(s) 785, 857, 923
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TseFI GTSAC 2 cut(s) 871, 1000
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 2 cut(s) 871, 1000
TspDTI ATGAA 2 cut(s) 239, 290
TspGWI ACGGA 1 cut(s) 516
VneI GTGCAC 1 cut(s) 621
VpaK11BI GGWCC 1 cut(s) 845
XhoI CTCGAG 2 cut(s) 216, 940
XmiI GTMKAC 1 cut(s) 392
XspI CTAG 3 cut(s) 6, 179, 342
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.