RchiOBHm_Chr5g0047031

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007736
Physical Location & Seq
Reverse (-)
43267755 .. 43274161
6407 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32497

Sequence Viewer

Length: 1056 bp
ATGGCTCTGGTACCCTCATCTGCGCCAGTGAACGAGAGCCCAGTGATCACCGAGGTTGACATGGGCGGCGACGACTCCACACCCACCGTCCGAGCCACCGTCGTCCAAGCCTCCACCGTCTTCTACGACACTCCCGCCACTCTAGATAAGGCTGAGAGATTGTTGGCTGAAGCAGCTGGGTATGGATCGCAGCTGGTTGTGTTTCCGGAAGCATTTGTGGGAGGGTATCCGCGAGGCTCCAATTTCGGGGTTGCGATTGGGAACAGAACTGCCAAGGGGAAAGAAGAGTTCCGAAAGTATCATGCCGCGGCCATTACTGTGCCTGGTCCTGAAGTTGACCGATTGGCATCAATGGCTGGAAAATATAAGGTATTCTTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACACTTTATTGTACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCACCGAAAAATAATGCCAACAGCATTAGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCCTGTGTTTGATACTCCAATAGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCACTTTTAAGGACAGCAATGTATGCTAAAGGCATTGAAATATATTGTGCTCCTACAGCTGATTCTAGGGATCTATGGCAAGCGTCAATGACCCATATTGCTTTAGAAGGTGGGTGCTTTGTCTTATCAGCTAACCAATTCTGTCGAAGGAAAGACTATCCCCCTCCACCAGAGTATATATTTTCAGGTACGGAAGAGGAACTTACTCCTGATTCTGTTGTCTGTGCTGGAGGCAGTGTCATCATATCACCATCAGGGACTATACTTGCTGGGCCCAATTATGACGGGGAGGCACTCATCTCAGCAGATCTTGATATTGGAGATATAGCAAGGGCGAAGTTTGATTTCGATGTGGTAGGACACTATGCACGGCCTGAAGTGCTTAGCTTGATTGTGAGGGACCATCCAGCAAATCCGGTTACTTTTACATCAGCCTCAGCGAAGACAGAAGTCTCAGACAAATCATAA

Protein Analysis

351

Amino Acids

37.69

Weight (kDa)

5.02

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 31 - 309 2.2e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 10
AccB7I CCANNNNNTGG 1 cut(s) 573
AccI GTMKAC 1 cut(s) 407
AccII CGCG 2 cut(s) 232, 308
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 5 cut(s) 66, 135, 230, 306, 308
AclWI GGATC 4 cut(s) 193, 513, 526, 666
AcoI YGGCCR 1 cut(s) 309
AcuI CTGAAG 3 cut(s) 189, 351, 984
AfaI GTAC 3 cut(s) 12, 421, 778
AfiI CCNNNNNNNGG 2 cut(s) 246, 573
AgsI TTSAA 1 cut(s) 626
AjnI CCWGG 2 cut(s) 322, 453
AluBI AGCT 5 cut(s) 176, 193, 647, 719, 975
AluI AGCT 5 cut(s) 176, 193, 647, 719, 975
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 1045
AlwI GGATC 4 cut(s) 193, 513, 526, 666
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 3 cut(s) 309, 860, 959
ApaI GGGCCC 1 cut(s) 864
ApeKI GCWGC 3 cut(s) 173, 190, 563
Asp718I GGTACC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 4 cut(s) 326, 860, 861, 988
AsuHPI GGTGA 2 cut(s) 40, 828
AvaII GGWCC 2 cut(s) 326, 988
BaeGI GKGCMC 1 cut(s) 864
BamHI GGATCC 1 cut(s) 518
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 2 cut(s) 41, 864
BbsI GAAGAC 2 cut(s) 112, 1037
Bbv12I GWGCWC 1 cut(s) 640
BbvCI CCTCAGC 1 cut(s) 1024
BbvI GCAGC 3 cut(s) 185, 202, 550
BccI CCATC 3 cut(s) 509, 847, 999
BceAI ACGGC 1 cut(s) 974
BciT130I CCWGG 2 cut(s) 324, 455
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 1045
BfaI CTAG 2 cut(s) 143, 654
BfmI CTRYAG 1 cut(s) 642
BfuI GTATCC 1 cut(s) 237
BglII AGATCT 1 cut(s) 895
BisI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
BlpI GCTNAGC 1 cut(s) 971
BlsI GCNGC 6 cut(s) 68, 175, 192, 307, 310, 565
Bme1390I CCNGG 2 cut(s) 324, 455
Bme18I GGWCC 2 cut(s) 326, 988
BmgT120I GGNCC 4 cut(s) 326, 860, 861, 988
BmiI GGNNCC 5 cut(s) 12, 238, 520, 862, 989
BmrFI CCNGG 2 cut(s) 324, 455
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 2 cut(s) 356, 576
BmuI ACTGGG 1 cut(s) 35
BoxI GACNNNNGTC 1 cut(s) 1037
BpiI GAAGAC 2 cut(s) 112, 1037
BpmI CTGGAG 1 cut(s) 837
Bpu10I CCTNAGC 1 cut(s) 1024
Bpu1102I GCTNAGC 1 cut(s) 971
BpuEI CTTGAG 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 346
BsaJI CCNNGG 4 cut(s) 51, 273, 306, 454
BsaWI WCCGGW 2 cut(s) 205, 1003
BsaXI ACNNNNNCTCC 2 cut(s) 810, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 573
Bse1I ACTGG 2 cut(s) 26, 41
Bse3DI GCAATG 1 cut(s) 612
Bse8I GATNNNNATC 1 cut(s) 346
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 2 cut(s) 324, 455
BseDI CCNNGG 4 cut(s) 51, 273, 306, 454
BseGI GGATG 1 cut(s) 991
BseJI GATNNNNATC 1 cut(s) 346
BseLI CCNNNNNNNGG 2 cut(s) 246, 573
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 4 cut(s) 144, 903, 1038, 1056
BseNI ACTGG 2 cut(s) 26, 41
BseSI GKGCMC 1 cut(s) 864
BseXI GCAGC 3 cut(s) 185, 202, 550
BseYI CCCAGC 2 cut(s) 176, 857
Bsh1236I CGCG 2 cut(s) 232, 308
BshFI GGCC 3 cut(s) 311, 862, 961
BshNI GGYRCC 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 2 cut(s) 206, 1004
BslFI GGGAC 2 cut(s) 859, 1001
BslI CCNNNNNNNGG 2 cut(s) 246, 573
BsmAI GTCTC 1 cut(s) 1045
BsmFI GGGAC 2 cut(s) 859, 1001
BsnI GGCC 3 cut(s) 311, 862, 961
Bsp120I GGGCCC 1 cut(s) 860
Bsp1286I GDGCHC 3 cut(s) 41, 640, 864
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 5 cut(s) 45, 185, 518, 658, 895
Bsp1720I GCTNAGC 1 cut(s) 971
BspACI CCGC 5 cut(s) 66, 135, 230, 306, 308
BspANI GGCC 3 cut(s) 311, 862, 961
BspCNI CTCAG 4 cut(s) 145, 902, 1037, 1055
BspEI TCCGGA 1 cut(s) 205
BspFNI CGCG 2 cut(s) 232, 308
BspLI GGNNCC 5 cut(s) 12, 238, 520, 862, 989
BspPI GGATC 4 cut(s) 193, 513, 526, 666
BspT107I GGYRCC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 612
BsrI ACTGG 2 cut(s) 26, 41
BssECI CCNNGG 4 cut(s) 51, 273, 306, 454
BssMI GATC 5 cut(s) 45, 185, 518, 658, 895
BssNAI GTATAC 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 324, 455
Bst4CI ACNGT 5 cut(s) 88, 100, 118, 319, 424
Bst6I CTCTTC 2 cut(s) 279, 777
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 669
BstDEI CTNAG 5 cut(s) 153, 889, 971, 1024, 1042
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 991
BstFNI CGCG 2 cut(s) 232, 308
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 5 cut(s) 48, 188, 521, 661, 898
BstMAI GTCTC 1 cut(s) 1045
BstMBI GATC 5 cut(s) 45, 185, 518, 658, 895
BstMWI GCNNNNNNNGC 5 cut(s) 173, 353, 611, 644, 967
BstNI CCWGG 2 cut(s) 324, 455
BstPAI GACNNNNGTC 1 cut(s) 1037
BstSCI CCNGG 2 cut(s) 322, 453
BstSFI CTRYAG 1 cut(s) 642
BstSLI GKGCMC 1 cut(s) 864
BstUI CGCG 2 cut(s) 232, 308
BstV1I GCAGC 3 cut(s) 185, 202, 550
BstV2I GAAGAC 2 cut(s) 112, 1037
BstX2I RGATCY 3 cut(s) 518, 658, 895
BstYI RGATCY 3 cut(s) 518, 658, 895
BstZ17I GTATAC 1 cut(s) 408
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 3 cut(s) 311, 862, 961
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 991
BtsI GCAGTG 1 cut(s) 829
BtsIMutI CAGTG 3 cut(s) 33, 48, 829
Cac8I GCNNGC 1 cut(s) 669
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 4 cut(s) 326, 860, 861, 988
Cfr42I CCGCGG 1 cut(s) 309
CseI GACGC 1 cut(s) 660
Csp6I GTAC 3 cut(s) 11, 420, 777
CviAII CATG 2 cut(s) 61, 302
CviQI GTAC 3 cut(s) 11, 420, 777
DdeI CTNAG 5 cut(s) 153, 889, 971, 1024, 1042
DpnI GATC 5 cut(s) 47, 187, 520, 660, 897
DpnII GATC 5 cut(s) 45, 185, 518, 658, 895
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 2 cut(s) 279, 777
EarI CTCTTC 2 cut(s) 279, 777
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 2 cut(s) 41, 864
Eco47I GGWCC 2 cut(s) 326, 988
Eco57I CTGAAG 3 cut(s) 189, 351, 984
EcoRII CCWGG 2 cut(s) 322, 453
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 2 cut(s) 41, 864
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 64, 305
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 774, 806
FaqI GGGAC 2 cut(s) 859, 1001
FatI CATG 2 cut(s) 60, 301
FauI CCCGC 1 cut(s) 142
FbaI TGATCA 1 cut(s) 45
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
FokI GGATG 1 cut(s) 978
FriOI GRGCYC 2 cut(s) 41, 864
Fsp4HI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
FspBI CTAG 2 cut(s) 143, 654
GlaI GCGC 1 cut(s) 24
GluI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
GsaI CCCAGC 2 cut(s) 180, 861
GsuI CTGGAG 1 cut(s) 837
HaeIII GGCC 3 cut(s) 311, 862, 961
HapII CCGG 2 cut(s) 206, 1004
HgaI GACGC 1 cut(s) 660
HhaI GCGC 1 cut(s) 25
Hin1II CATG 2 cut(s) 64, 305
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 2 cut(s) 58, 337
HindII GTYRAC 2 cut(s) 58, 337
HinfI GANTC 5 cut(s) 74, 437, 495, 650, 800
HpaII CCGG 2 cut(s) 206, 1004
HphI GGTGA 2 cut(s) 40, 828
Hpy166II GTNNAC 4 cut(s) 31, 58, 337, 408
Hpy188I TCNGA 3 cut(s) 92, 293, 1045
Hpy188III TCNNGA 5 cut(s) 143, 206, 329, 797, 899
Hpy8I GTNNAC 4 cut(s) 31, 58, 337, 408
Hpy99I CGWCG 2 cut(s) 74, 104
HpyAV CCTTC 3 cut(s) 395, 689, 729
HpyCH4III ACNGT 5 cut(s) 88, 100, 118, 319, 424
HpyCH4V TGCA 1 cut(s) 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 353, 611, 644, 967
HpyF3I CTNAG 5 cut(s) 153, 889, 971, 1024, 1042
Hsp92II CATG 2 cut(s) 64, 305
HspAI GCGC 1 cut(s) 23
Kpn2I TCCGGA 1 cut(s) 205
KpnI GGTACC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 45
KspI CCGCGG 1 cut(s) 309
Kzo9I GATC 5 cut(s) 45, 185, 518, 658, 895
LmnI GCTCC 2 cut(s) 242, 643
Lsp1109I GCAGC 3 cut(s) 185, 202, 550
LweI GCATC 2 cut(s) 356, 576
MaeI CTAG 2 cut(s) 143, 654
MaeIII GTNAC 1 cut(s) 1006
MalI GATC 5 cut(s) 47, 187, 520, 660, 897
MboI GATC 5 cut(s) 45, 185, 518, 658, 895
MboII GAAGA 4 cut(s) 112, 296, 794, 1042
MflI RGATCY 3 cut(s) 518, 658, 895
MhlI GDGCHC 3 cut(s) 41, 640, 864
MluCI AATT 4 cut(s) 241, 525, 725, 865
MlyI GAGTC 1 cut(s) 68
MroI TCCGGA 1 cut(s) 205
MseI TTAA 1 cut(s) 596
MslI CAYNNNNRTG 1 cut(s) 317
MspA1I CMGCKG 4 cut(s) 176, 193, 308, 647
MspI CCGG 2 cut(s) 206, 1004
MspR9I CCNGG 2 cut(s) 324, 455
MvaI CCWGG 2 cut(s) 324, 455
MvnI CGCG 2 cut(s) 232, 308
MwoI GCNNNNNNNGC 5 cut(s) 173, 353, 611, 644, 967
NdeII GATC 5 cut(s) 45, 185, 518, 658, 895
NlaIII CATG 2 cut(s) 64, 305
NlaIV GGNNCC 5 cut(s) 12, 238, 520, 862, 989
PfeI GAWTC 4 cut(s) 437, 495, 650, 800
PflMI CCANNNNNTGG 1 cut(s) 573
PkrI GCNGC 6 cut(s) 68, 175, 192, 307, 310, 565
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PshAI GACNNNNGTC 1 cut(s) 1037
Psp6I CCWGG 2 cut(s) 322, 453
PspFI CCCAGC 2 cut(s) 176, 857
PspGI CCWGG 2 cut(s) 322, 453
PspN4I GGNNCC 5 cut(s) 12, 238, 520, 862, 989
PspOMI GGGCCC 1 cut(s) 860
PspPI GGNCC 4 cut(s) 326, 860, 861, 988
PsuI RGATCY 3 cut(s) 518, 658, 895
PvuII CAGCTG 3 cut(s) 176, 193, 647
RsaI GTAC 3 cut(s) 12, 421, 778
RsaNI GTAC 3 cut(s) 11, 420, 777
RseI CAYNNNNRTG 1 cut(s) 317
SacII CCGCGG 1 cut(s) 309
SaqAI TTAA 1 cut(s) 596
SatI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
Sau3AI GATC 5 cut(s) 45, 185, 518, 658, 895
Sau96I GGNCC 4 cut(s) 326, 860, 861, 988
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 324, 455
SduI GDGCHC 3 cut(s) 41, 640, 864
SfaNI GCATC 2 cut(s) 356, 576
SfcI CTRYAG 1 cut(s) 642
Sfr303I CCGCGG 1 cut(s) 309
SgrBI CCGCGG 1 cut(s) 309
SinI GGWCC 2 cut(s) 326, 988
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 4 cut(s) 241, 525, 725, 865
SsiI CCGC 5 cut(s) 66, 135, 230, 306, 308
SspMI CTAG 2 cut(s) 143, 654
StyD4I CCNGG 2 cut(s) 322, 453
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 5 cut(s) 88, 100, 118, 319, 424
TaqI TCGA 2 cut(s) 733, 936
TaqII GACCGA 1 cut(s) 354
TasI AATT 4 cut(s) 241, 525, 725, 865
TatI WGTACW 1 cut(s) 419
TauI GCSGC 3 cut(s) 69, 308, 311
TfiI GAWTC 4 cut(s) 437, 495, 650, 800
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TscAI CASTG 3 cut(s) 33, 48, 829
TseI GCWGC 3 cut(s) 173, 190, 563
TspGWI ACGGA 1 cut(s) 794
TspRI CASTG 3 cut(s) 33, 48, 829
Van91I CCANNNNNTGG 1 cut(s) 573
VpaK11BI GGWCC 2 cut(s) 326, 988
XbaI TCTAGA 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 2 cut(s) 143, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.