Rw5G029210

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007736
Physical Location & Seq
Reverse (-)
44610910 .. 44617100
6191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029210.1

Sequence Viewer

Length: 1143 bp
ATGCAATGTGCGAAAGTAAAGACAAAGAGATTCCAGCCTCCAAATTCGAATACAAAAACTGATTGGTGTGAAACTGAGAGAGACCAAATGGCTCTGGTACCCTCATCTGCGCCAGTGCACGAGAGCCCAGTGATCACCGAGGTTGACATGGGCGGCGACGGCTCCACACCCACCGTCCGAGCCACCGTCGTCCAAGCCTCCACCGTCTTCTACGACACTCCCGCCACTCTAGATAAGGCTGAGAGATTGTTGGCTGAAGCAGCTGGGTATGGATCGCAGCTGGTTGTGTTTCCGGAAGCATTTGTGGGTGGGTATCCGCGAGGCTCCAATTTCGGGGTTGCCATTGGGAACAGAACCGCCAAGGGGAAAGAAGAGTTCCGAAAGTATCATGCCGCAGCCATTACTGTGCCTGGTCCTGAAGTTGACCGATTGGCATCAATGGCTGGAAAATATAAGGTATTCTTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACACTTTATTGTACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCACCGAAAAATAATGCCAACAGCATTAGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCCTGTGTTTGATACTCCAATTGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCACTTTTAAGGACAGCAATGTATGCTAAAGGCATTGAAATATATTGTGCTCCTACAGCTGATTCTAGGGATCTATGGCAAGCGTCAATGACCCATATTGCTTTAGAAGGTGGGTGCTTTGTCTTATCAGCTAACCAATTCTGTCGAAGGAAAGACTATCCCCCTCCACCAGAGTACATATTTTCAGGCACGGAAGAGGAACTTACTCCTGATTCTGTTGTCTGTGCTGGAGGCAGTGTCATCATATCACCATCAGGGACTATACTTGCTGGGCCCAATTATGACGGGGAGGCACTCATCTCAGCAGATCTTGATATTGGAGAAATAGCAAGGGCGAAGTTTGATTTCGATGTGGTAGGACACTATGCACGGCCTGAAGTGCTTAGCTTGATTGTGAGGGACCATCCAGCAAATCCGGTTACTTTTACATCAGCCTCAGCGAAGACAGAAGTCTCAGACAAATCATAA

Protein Analysis

380

Amino Acids

41.12

Weight (kDa)

5.41

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 60 - 338 4.1e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 97
AccB7I CCANNNNNTGG 1 cut(s) 660
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 319
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 5 cut(s) 153, 222, 317, 357, 393
AclWI GGATC 4 cut(s) 280, 600, 613, 753
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 3 cut(s) 276, 438, 1071
AfaI GTAC 3 cut(s) 99, 508, 851
AfiI CCNNNNNNNGG 3 cut(s) 333, 363, 660
AgsI TTSAA 1 cut(s) 713
AjnI CCWGG 2 cut(s) 409, 540
AluBI AGCT 5 cut(s) 263, 280, 734, 806, 1062
AluI AGCT 5 cut(s) 263, 280, 734, 806, 1062
Alw21I GWGCWC 2 cut(s) 120, 727
Alw26I GTCTC 2 cut(s) 75, 1132
Alw44I GTGCAC 1 cut(s) 116
AlwI GGATC 4 cut(s) 280, 600, 613, 753
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 2 cut(s) 947, 1046
ApaI GGGCCC 1 cut(s) 951
ApaLI GTGCAC 1 cut(s) 116
ApeKI GCWGC 4 cut(s) 260, 277, 395, 650
ApoI RAATTY 1 cut(s) 43
Asp718I GGTACC 1 cut(s) 97
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 4 cut(s) 413, 947, 948, 1075
AsuHPI GGTGA 2 cut(s) 127, 915
AsuII TTCGAA 1 cut(s) 47
AvaII GGWCC 2 cut(s) 413, 1075
BaeGI GKGCMC 2 cut(s) 120, 951
BamHI GGATCC 1 cut(s) 605
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 2 cut(s) 128, 951
BauI CACGAG 1 cut(s) 119
BbsI GAAGAC 2 cut(s) 199, 1124
Bbv12I GWGCWC 2 cut(s) 120, 727
BbvCI CCTCAGC 1 cut(s) 1111
BbvI GCAGC 4 cut(s) 272, 289, 407, 637
BccI CCATC 3 cut(s) 596, 934, 1086
BceAI ACGGC 2 cut(s) 175, 1061
BciT130I CCWGG 2 cut(s) 411, 542
BciVI GTATCC 1 cut(s) 324
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 75, 1132
BfaI CTAG 2 cut(s) 230, 741
BfmI CTRYAG 1 cut(s) 729
BfuI GTATCC 1 cut(s) 324
BglII AGATCT 1 cut(s) 982
BisI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
BlpI GCTNAGC 1 cut(s) 1058
BlsI GCNGC 6 cut(s) 155, 262, 279, 394, 397, 652
Bme1390I CCNGG 2 cut(s) 411, 542
Bme18I GGWCC 2 cut(s) 413, 1075
BmgT120I GGNCC 4 cut(s) 413, 947, 948, 1075
BmiI GGNNCC 6 cut(s) 99, 163, 325, 607, 949, 1076
BmrFI CCNGG 2 cut(s) 411, 542
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 2 cut(s) 443, 663
BmuI ACTGGG 1 cut(s) 122
BoxI GACNNNNGTC 1 cut(s) 1124
BpiI GAAGAC 2 cut(s) 199, 1124
BpmI CTGGAG 1 cut(s) 924
Bpu10I CCTNAGC 1 cut(s) 1111
Bpu1102I GCTNAGC 1 cut(s) 1058
Bpu14I TTCGAA 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 513
BsaBI GATNNNNATC 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 138, 360, 541
BsaWI WCCGGW 2 cut(s) 292, 1090
BsaXI ACNNNNNCTCC 2 cut(s) 897, 927
Bsc4I CCNNNNNNNGG 3 cut(s) 333, 363, 660
Bse1I ACTGG 2 cut(s) 113, 128
Bse3DI GCAATG 2 cut(s) 11, 699
Bse8I GATNNNNATC 1 cut(s) 433
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 2 cut(s) 411, 542
BseDI CCNNGG 3 cut(s) 138, 360, 541
BseGI GGATG 1 cut(s) 1078
BseJI GATNNNNATC 1 cut(s) 433
BseLI CCNNNNNNNGG 3 cut(s) 333, 363, 660
BseMI GCAATG 2 cut(s) 11, 699
BseMII CTCAG 5 cut(s) 66, 231, 990, 1125, 1143
BseNI ACTGG 2 cut(s) 113, 128
BseSI GKGCMC 2 cut(s) 120, 951
BseXI GCAGC 4 cut(s) 272, 289, 407, 637
BseYI CCCAGC 2 cut(s) 263, 944
Bsh1236I CGCG 1 cut(s) 319
BshFI GGCC 2 cut(s) 949, 1048
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 2 cut(s) 120, 727
BsiSI CCGG 2 cut(s) 293, 1091
BslFI GGGAC 2 cut(s) 946, 1088
BslI CCNNNNNNNGG 3 cut(s) 333, 363, 660
BsmAI GTCTC 2 cut(s) 75, 1132
BsmFI GGGAC 2 cut(s) 946, 1088
BsnI GGCC 2 cut(s) 949, 1048
Bso31I GGTCTC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 47
Bsp120I GGGCCC 1 cut(s) 947
Bsp1286I GDGCHC 4 cut(s) 120, 128, 727, 951
Bsp13I TCCGGA 1 cut(s) 292
Bsp143I GATC 5 cut(s) 132, 272, 605, 745, 982
Bsp1720I GCTNAGC 1 cut(s) 1058
BspACI CCGC 5 cut(s) 153, 222, 317, 357, 393
BspANI GGCC 2 cut(s) 949, 1048
BspCNI CTCAG 5 cut(s) 67, 232, 989, 1124, 1142
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 6 cut(s) 99, 163, 325, 607, 949, 1076
BspPI GGATC 4 cut(s) 280, 600, 613, 753
BspT104I TTCGAA 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 11, 699
BsrI ACTGG 2 cut(s) 113, 128
BssECI CCNNGG 3 cut(s) 138, 360, 541
BssMI GATC 5 cut(s) 132, 272, 605, 745, 982
BssNAI GTATAC 1 cut(s) 495
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 1 cut(s) 360
Bst1107I GTATAC 1 cut(s) 495
Bst2BI CACGAG 1 cut(s) 119
Bst2UI CCWGG 2 cut(s) 411, 542
Bst4CI ACNGT 5 cut(s) 175, 187, 205, 406, 511
Bst6I CTCTTC 2 cut(s) 366, 864
BstAPI GCANNNNNTGC 1 cut(s) 698
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 6 cut(s) 75, 240, 976, 1058, 1111, 1129
BstF5I GGATG 1 cut(s) 1078
BstFNI CGCG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 5 cut(s) 135, 275, 608, 748, 985
BstMAI GTCTC 2 cut(s) 75, 1132
BstMBI GATC 5 cut(s) 132, 272, 605, 745, 982
BstMWI GCNNNNNNNGC 6 cut(s) 159, 260, 440, 698, 731, 1054
BstNI CCWGG 2 cut(s) 411, 542
BstPAI GACNNNNGTC 1 cut(s) 1124
BstSCI CCNGG 2 cut(s) 409, 540
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 2 cut(s) 120, 951
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 4 cut(s) 272, 289, 407, 637
BstV2I GAAGAC 2 cut(s) 199, 1124
BstX2I RGATCY 3 cut(s) 605, 745, 982
BstYI RGATCY 3 cut(s) 605, 745, 982
BstZ17I GTATAC 1 cut(s) 495
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 2 cut(s) 949, 1048
BtsCI GGATG 1 cut(s) 1078
BtsI GCAGTG 1 cut(s) 916
BtsIMutI CAGTG 3 cut(s) 120, 135, 916
Cac8I GCNNGC 1 cut(s) 756
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 4 cut(s) 413, 947, 948, 1075
CseI GACGC 1 cut(s) 747
Csp6I GTAC 3 cut(s) 98, 507, 850
CviAII CATG 2 cut(s) 148, 389
CviQI GTAC 3 cut(s) 98, 507, 850
DdeI CTNAG 6 cut(s) 75, 240, 976, 1058, 1111, 1129
DpnI GATC 5 cut(s) 134, 274, 607, 747, 984
DpnII GATC 5 cut(s) 132, 272, 605, 745, 982
Eam1104I CTCTTC 2 cut(s) 366, 864
EarI CTCTTC 2 cut(s) 366, 864
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 2 cut(s) 128, 951
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 413, 1075
Eco57I CTGAAG 3 cut(s) 276, 438, 1071
EcoRII CCWGG 2 cut(s) 409, 540
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 2 cut(s) 128, 951
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 2 cut(s) 151, 392
FalI AAGNNNNNCTT 4 cut(s) 446, 478, 861, 893
FaqI GGGAC 2 cut(s) 946, 1088
FatI CATG 2 cut(s) 147, 388
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 1 cut(s) 132
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
FokI GGATG 1 cut(s) 1065
FriOI GRGCYC 2 cut(s) 128, 951
Fsp4HI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
FspBI CTAG 2 cut(s) 230, 741
GlaI GCGC 1 cut(s) 111
GluI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
GsaI CCCAGC 2 cut(s) 267, 948
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 2 cut(s) 949, 1048
HapII CCGG 2 cut(s) 293, 1091
HgaI GACGC 1 cut(s) 747
HhaI GCGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 151, 392
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 2 cut(s) 145, 424
HindII GTYRAC 2 cut(s) 145, 424
HinfI GANTC 5 cut(s) 30, 524, 582, 737, 887
HpaII CCGG 2 cut(s) 293, 1091
HphI GGTGA 2 cut(s) 127, 915
Hpy166II GTNNAC 4 cut(s) 118, 145, 424, 495
Hpy188I TCNGA 3 cut(s) 179, 380, 1132
Hpy188III TCNNGA 5 cut(s) 230, 293, 416, 884, 986
Hpy8I GTNNAC 4 cut(s) 118, 145, 424, 495
Hpy99I CGWCG 2 cut(s) 161, 191
HpyAV CCTTC 3 cut(s) 482, 776, 816
HpyCH4III ACNGT 5 cut(s) 175, 187, 205, 406, 511
HpyCH4V TGCA 3 cut(s) 4, 118, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 159, 260, 440, 698, 731, 1054
HpyF3I CTNAG 6 cut(s) 75, 240, 976, 1058, 1111, 1129
Hsp92II CATG 2 cut(s) 151, 392
HspAI GCGC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 292
KpnI GGTACC 1 cut(s) 101
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 5 cut(s) 132, 272, 605, 745, 982
LmnI GCTCC 3 cut(s) 167, 329, 730
Lsp1109I GCAGC 4 cut(s) 272, 289, 407, 637
LweI GCATC 2 cut(s) 443, 663
MaeI CTAG 2 cut(s) 230, 741
MaeIII GTNAC 1 cut(s) 1093
MalI GATC 5 cut(s) 134, 274, 607, 747, 984
MboI GATC 5 cut(s) 132, 272, 605, 745, 982
MboII GAAGA 4 cut(s) 199, 383, 881, 1129
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 3 cut(s) 605, 745, 982
MhlI GDGCHC 4 cut(s) 120, 128, 727, 951
MluCI AATT 6 cut(s) 43, 328, 612, 633, 812, 952
MroI TCCGGA 1 cut(s) 292
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 3 cut(s) 263, 280, 734
MspI CCGG 2 cut(s) 293, 1091
MspR9I CCNGG 2 cut(s) 411, 542
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 2 cut(s) 411, 542
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 6 cut(s) 159, 260, 440, 698, 731, 1054
NdeII GATC 5 cut(s) 132, 272, 605, 745, 982
NlaIII CATG 2 cut(s) 151, 392
NlaIV GGNNCC 6 cut(s) 99, 163, 325, 607, 949, 1076
NspV TTCGAA 1 cut(s) 47
PfeI GAWTC 5 cut(s) 30, 524, 582, 737, 887
PflMI CCANNNNNTGG 1 cut(s) 660
PkrI GCNGC 6 cut(s) 155, 262, 279, 394, 397, 652
PshAI GACNNNNGTC 1 cut(s) 1124
Psp6I CCWGG 2 cut(s) 409, 540
PspFI CCCAGC 2 cut(s) 263, 944
PspGI CCWGG 2 cut(s) 409, 540
PspN4I GGNNCC 6 cut(s) 99, 163, 325, 607, 949, 1076
PspOMI GGGCCC 1 cut(s) 947
PspPI GGNCC 4 cut(s) 413, 947, 948, 1075
PsuI RGATCY 3 cut(s) 605, 745, 982
PvuII CAGCTG 3 cut(s) 263, 280, 734
RsaI GTAC 3 cut(s) 99, 508, 851
RsaNI GTAC 3 cut(s) 98, 507, 850
RseI CAYNNNNRTG 1 cut(s) 404
SaqAI TTAA 1 cut(s) 683
SatI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
Sau3AI GATC 5 cut(s) 132, 272, 605, 745, 982
Sau96I GGNCC 4 cut(s) 413, 947, 948, 1075
ScrFI CCNGG 2 cut(s) 411, 542
SduI GDGCHC 4 cut(s) 120, 128, 727, 951
SfaNI GCATC 2 cut(s) 443, 663
SfcI CTRYAG 1 cut(s) 729
SfuI TTCGAA 1 cut(s) 47
SinI GGWCC 2 cut(s) 413, 1075
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
Sse9I AATT 6 cut(s) 43, 328, 612, 633, 812, 952
SsiI CCGC 5 cut(s) 153, 222, 317, 357, 393
SspMI CTAG 2 cut(s) 230, 741
StyD4I CCNGG 2 cut(s) 409, 540
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 5 cut(s) 175, 187, 205, 406, 511
TaqI TCGA 3 cut(s) 47, 820, 1023
TaqII GACCGA 1 cut(s) 441
TasI AATT 6 cut(s) 43, 328, 612, 633, 812, 952
TatI WGTACW 2 cut(s) 506, 849
TauI GCSGC 2 cut(s) 156, 395
TfiI GAWTC 5 cut(s) 30, 524, 582, 737, 887
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 3 cut(s) 120, 135, 916
TseI GCWGC 4 cut(s) 260, 277, 395, 650
TspGWI ACGGA 1 cut(s) 881
TspRI CASTG 3 cut(s) 120, 135, 916
Van91I CCANNNNNTGG 1 cut(s) 660
VneI GTGCAC 1 cut(s) 116
VpaK11BI GGWCC 2 cut(s) 413, 1075
XapI RAATTY 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 2 cut(s) 230, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.