RLG00000005908

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018005
Physical Location & Seq
Forward (+)
2744121 .. 2745507
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005908

Sequence Viewer

Length: 366 bp
ATGCCCGACAACCTCTACTACAGCGACAACGAAACCTTGATCTCGATCTCCTATTCCGATAAGGCTGAGAGATTGTTGGCTGAAGCAGCGGGTATGGATCGCACCGGGTTATGTTTCCGGAAGCGTTTCTGGGGAGGGTATCCACGTGGCTCCAATTTTAGGGTTGCGATTGGGAACAGAACTGCCAAGGGGAAAAAAGAGTTCCGAAAGTATCATGCCGCAGCCATTAATGTGCCTGGCTTATGTCCAGCAACTAGAAACAAGCCGTTTAAAACTGGTGCAACTGGAGCTGGAACTGGACAGGACTGGAAAACAGGTAGCCTATGGAGGCAGTTCATCCTTAATTTGGTGGGGGGACTGGGCTAG

Protein Analysis

122

Amino Acids

13.32

Weight (kDa)

10.01

Isoelectric Point (pI)

20.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018005)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25810 FvH4_3g25810 FvH4_3g25810
pyrus_communis pycom11g14250
rosa_laevigata RLG00000001790 RLG00000005908 RLG00000029485
rosa_roxburghii Rroxscaffold_3G00241990
rosa_samantha Rh1CG027800 Rh5DG332500
rosa_wichuraiana Rw6G020870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 117
AciI CCGC 2 cut(s) 89, 219
AclWI GGATC 1 cut(s) 105
AcuI CTGAAG 1 cut(s) 102
AcvI CACGTG 1 cut(s) 146
AfiI CCNNNNNNNGG 2 cut(s) 159, 346
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
AlwI GGATC 1 cut(s) 105
Aor13HI TCCGGA 1 cut(s) 117
ApeKI GCWGC 2 cut(s) 86, 221
AseI ATTAAT 1 cut(s) 228
Asp700I GAANNNNTTC 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 106
BbrPI CACGTG 1 cut(s) 146
BbvI GCAGC 2 cut(s) 98, 233
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 237
BciVI GTATCC 1 cut(s) 150
BcnI CCSGG 1 cut(s) 106
BfaI CTAG 2 cut(s) 255, 364
BfmI CTRYAG 1 cut(s) 19
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 3 cut(s) 87, 219, 222
BlsI GCNGC 3 cut(s) 88, 220, 223
Bme1390I CCNGG 2 cut(s) 106, 237
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 2 cut(s) 106, 237
BpmI CTGGAG 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 106
BsaAI YACGTR 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 117
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 346
Bse1I ACTGG 5 cut(s) 280, 289, 301, 311, 363
Bse8I GATNNNNATC 1 cut(s) 44
BseAI TCCGGA 1 cut(s) 117
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 2 cut(s) 159, 346
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 5 cut(s) 280, 289, 301, 311, 363
BseXI GCAGC 2 cut(s) 98, 233
BsiSI CCGG 2 cut(s) 105, 118
BslI CCNNNNNNNGG 2 cut(s) 159, 346
Bsp13I TCCGGA 1 cut(s) 117
Bsp143I GATC 3 cut(s) 39, 45, 97
BspACI CCGC 2 cut(s) 89, 219
BspCNI CTCAG 1 cut(s) 58
BspEI TCCGGA 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 151
BspPI GGATC 1 cut(s) 105
BsrI ACTGG 5 cut(s) 280, 289, 301, 311, 363
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 3 cut(s) 39, 45, 97
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 336
BstKTI GATC 3 cut(s) 42, 48, 100
BstMBI GATC 3 cut(s) 39, 45, 97
BstMWI GCNNNNNNNGC 2 cut(s) 86, 287
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 2 cut(s) 104, 235
BstSFI CTRYAG 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 98, 233
BsuI GTATCC 1 cut(s) 150
BtsCI GGATG 1 cut(s) 336
CviAII CATG 1 cut(s) 215
CviJI RGCY 9 cut(s) 65, 80, 150, 224, 240, 265, 290, 321, 363
CviKI_1 RGCY 9 cut(s) 65, 80, 150, 224, 240, 265, 290, 321, 363
DdeI CTNAG 1 cut(s) 66
DpnI GATC 3 cut(s) 41, 47, 99
DpnII GATC 3 cut(s) 39, 45, 97
DraI TTTAAA 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 102
Eco72I CACGTG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 1 cut(s) 218
FaiI YATR 5 cut(s) 95, 112, 216, 244, 325
FatI CATG 1 cut(s) 214
FauI CCCGC 1 cut(s) 82
Fnu4HI GCNGC 3 cut(s) 87, 219, 222
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 3 cut(s) 87, 219, 222
FspBI CTAG 2 cut(s) 255, 364
GluI GCNGC 3 cut(s) 87, 219, 222
GsuI CTGGAG 1 cut(s) 306
HapII CCGG 2 cut(s) 105, 118
Hin1II CATG 1 cut(s) 218
HpaII CCGG 2 cut(s) 105, 118
Hpy188I TCNGA 2 cut(s) 58, 206
Hpy188III TCNNGA 2 cut(s) 43, 118
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 287
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 39, 45, 97
LmnI GCTCC 2 cut(s) 155, 287
Lsp1109I GCAGC 2 cut(s) 98, 233
MaeI CTAG 2 cut(s) 255, 364
MaeII ACGT 1 cut(s) 145
MalI GATC 3 cut(s) 41, 47, 99
MboI GATC 3 cut(s) 39, 45, 97
MluCI AATT 2 cut(s) 154, 343
MnlI CCTC 3 cut(s) 23, 128, 321
MroI TCCGGA 1 cut(s) 117
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 3 cut(s) 228, 270, 342
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 105, 118
MspR9I CCNGG 2 cut(s) 106, 237
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 86, 287
NciI CCSGG 1 cut(s) 106
NdeII GATC 3 cut(s) 39, 45, 97
NlaIII CATG 1 cut(s) 218
NlaIV GGNNCC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 125
PkrI GCNGC 3 cut(s) 88, 220, 223
PmaCI CACGTG 1 cut(s) 146
PmlI CACGTG 1 cut(s) 146
Ppu21I YACGTR 1 cut(s) 146
PshBI ATTAAT 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 235
PspCI CACGTG 1 cut(s) 146
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 3 cut(s) 228, 270, 342
SatI GCNGC 3 cut(s) 87, 219, 222
Sau3AI GATC 3 cut(s) 39, 45, 97
ScrFI CCNGG 2 cut(s) 106, 237
SetI ASST 5 cut(s) 15, 38, 148, 292, 319
SfcI CTRYAG 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 2 cut(s) 154, 343
SsiI CCGC 2 cut(s) 89, 219
SspMI CTAG 2 cut(s) 255, 364
StyD4I CCNGG 2 cut(s) 104, 235
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 1 cut(s) 148
TaqI TCGA 1 cut(s) 44
TasI AATT 2 cut(s) 154, 343
TauI GCSGC 1 cut(s) 221
Tru1I TTAA 3 cut(s) 228, 270, 342
Tru9I TTAA 3 cut(s) 228, 270, 342
TseI GCWGC 2 cut(s) 86, 221
TspDTI ATGAA 1 cut(s) 325
VspI ATTAAT 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 2 cut(s) 255, 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.