Rroxscaffold_3G00241990

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0018005
Physical Location & Seq
Reverse (-)
34329493 .. 34332656
3164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00241990.1

Sequence Viewer

Length: 519 bp
ATGGGGAGGATCGGTCTTGGGGATGCGGAAAAGGTGTTTTCGGCCGGGTCGGGTCGGCGGAGGCGGGGAGCCTTCGATCCAACGTACGGGGCAGCGGCGGTGCAAAGGAGGACCACGGGGAGCATGGGCGGCGCACGGCGAATCGAGGAGGGCGGGTCGGGGGCGGAGGTGGCGGAGGCCGTCCAAAACACCGGGTCGGATAAAGGTGAGAGATTGTTGGTGAAGCGGCGGGTACGGATCACGGCGGGGTTGTGTTTCCGGAAGCATTTTTGGGGACGGTATCCACGCAGCTCCAATTTCGGGGTTGCGATTGGGAATAGAATTGCCAAGGGAAAAGAAGAGCTTCAAAAGTATCATGCCACAGCCATTAATGTGCCTGGCTTATGTCCAGGAACTAGAAACAAGCCGTTTAAAACTGGTGCAACTGGAGCTGGAACCGGACAGGACTGGAAAACAGGTGAGACTCGCATTTCATCGCCCATTTCCAATTTGTATAGGTCTGTGTCCATCTACCCTTAG

Protein Analysis

172

Amino Acids

18.45

Weight (kDa)

10.78

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018005)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25810 FvH4_3g25810 FvH4_3g25810
pyrus_communis pycom11g14250
rosa_laevigata RLG00000001790 RLG00000005908 RLG00000029485
rosa_roxburghii Rroxscaffold_3G00241990
rosa_samantha Rh1CG027800 Rh5DG332500
rosa_wichuraiana Rw6G020870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 258
AclWI GGATC 3 cut(s) 17, 71, 245
AcoI YGGCCR 1 cut(s) 42
AfaI GTAC 2 cut(s) 86, 234
AfiI CCNNNNNNNGG 2 cut(s) 86, 300
AgsI TTSAA 1 cut(s) 347
AjnI CCWGG 2 cut(s) 376, 388
AluBI AGCT 3 cut(s) 291, 343, 431
AluI AGCT 3 cut(s) 291, 343, 431
Alw26I GTCTC 1 cut(s) 455
AlwI GGATC 3 cut(s) 17, 71, 245
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 2 cut(s) 42, 177
ApeKI GCWGC 2 cut(s) 92, 288
AseI ATTAAT 1 cut(s) 369
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 111
AsuC2I CCSGG 2 cut(s) 46, 193
AsuHPI GGTGA 3 cut(s) 218, 232, 470
AvaII GGWCC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 104, 300
BccI CCATC 1 cut(s) 515
BceAI ACGGC 4 cut(s) 152, 164, 258, 391
BciT130I CCWGG 2 cut(s) 378, 390
BciVI GTATCC 1 cut(s) 291
BcnI CCSGG 2 cut(s) 46, 193
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 1 cut(s) 396
BfuI GTATCC 1 cut(s) 291
BisI GCNGC 5 cut(s) 93, 96, 130, 227, 289
BlsI GCNGC 5 cut(s) 94, 97, 131, 228, 290
Bme1390I CCNGG 4 cut(s) 46, 193, 378, 390
Bme18I GGWCC 1 cut(s) 111
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 2 cut(s) 70, 436
BmrFI CCNGG 4 cut(s) 46, 193, 378, 390
BmsI GCATC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 447
BpuMI CCSGG 2 cut(s) 46, 193
BsaJI CCNNGG 2 cut(s) 114, 327
BsaWI WCCGGW 2 cut(s) 258, 437
BsaXI ACNNNNNCTCC 3 cut(s) 28, 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 300
Bse1I ACTGG 3 cut(s) 421, 430, 452
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 2 cut(s) 378, 390
BseDI CCNNGG 2 cut(s) 114, 327
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 2 cut(s) 86, 300
BseNI ACTGG 3 cut(s) 421, 430, 452
BseRI GAGGAG 1 cut(s) 161
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 104, 300
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 44, 179
BsiEI CGRYCG 1 cut(s) 45
BsiSI CCGG 4 cut(s) 45, 192, 259, 438
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 86, 300
BsmAI GTCTC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 288
BsnI GGCC 2 cut(s) 44, 179
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 3 cut(s) 9, 76, 237
BspANI GGCC 2 cut(s) 44, 179
BspEI TCCGGA 1 cut(s) 258
BspLI GGNNCC 2 cut(s) 70, 436
BspPI GGATC 3 cut(s) 17, 71, 245
BspQI GCTCTTC 1 cut(s) 333
BsrI ACTGG 3 cut(s) 421, 430, 452
BssECI CCNNGG 2 cut(s) 114, 327
BssMI GATC 3 cut(s) 9, 76, 237
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 378, 390
Bst4CI ACNGT 1 cut(s) 279
Bst6I CTCTTC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 3 cut(s) 12, 79, 240
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 3 cut(s) 9, 76, 237
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 129, 170, 428
BstNI CCWGG 2 cut(s) 378, 390
BstSCI CCNGG 4 cut(s) 44, 191, 376, 388
BstV1I GCAGC 2 cut(s) 104, 300
BstZI CGGCCG 1 cut(s) 42
BsuI GTATCC 1 cut(s) 291
BsuRI GGCC 2 cut(s) 44, 179
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 1 cut(s) 28
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 111
Csp6I GTAC 2 cut(s) 85, 233
CviAII CATG 2 cut(s) 124, 356
CviJI RGCY 9 cut(s) 44, 71, 179, 291, 343, 365, 381, 406, 431
CviKI_1 RGCY 9 cut(s) 44, 71, 179, 291, 343, 365, 381, 406, 431
CviQI GTAC 2 cut(s) 85, 233
DdeI CTNAG 1 cut(s) 516
DpnI GATC 3 cut(s) 11, 78, 239
DpnII GATC 3 cut(s) 9, 76, 237
DraI TTTAAA 1 cut(s) 412
EaeI YGGCCR 1 cut(s) 42
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 3 cut(s) 73, 179, 188
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 111
Eco52I CGGCCG 1 cut(s) 42
EcoRII CCWGG 2 cut(s) 376, 388
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 2 cut(s) 127, 359
FaiI YATR 4 cut(s) 125, 357, 385, 495
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 1 cut(s) 288
FatI CATG 2 cut(s) 123, 355
FauI CCCGC 4 cut(s) 57, 146, 222, 238
Fnu4HI GCNGC 5 cut(s) 93, 96, 130, 227, 289
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 5 cut(s) 93, 96, 130, 227, 289
FspBI CTAG 1 cut(s) 396
GlaI GCGC 1 cut(s) 133
GluI GCNGC 5 cut(s) 93, 96, 130, 227, 289
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 2 cut(s) 44, 179
HapII CCGG 4 cut(s) 45, 192, 259, 438
HhaI GCGC 1 cut(s) 134
Hin1II CATG 2 cut(s) 127, 359
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 2 cut(s) 141, 463
HpaII CCGG 4 cut(s) 45, 192, 259, 438
HphI GGTGA 3 cut(s) 218, 232, 470
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 1 cut(s) 279
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 2 cut(s) 103, 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 170, 428
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 2 cut(s) 127, 359
HspAI GCGC 1 cut(s) 132
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 3 cut(s) 9, 76, 237
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 4 cut(s) 68, 120, 296, 428
Lsp1109I GCAGC 2 cut(s) 104, 300
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 83
MalI GATC 3 cut(s) 11, 78, 239
MboI GATC 3 cut(s) 9, 76, 237
MboII GAAGA 1 cut(s) 350
MluCI AATT 3 cut(s) 295, 321, 487
MlyI GAGTC 1 cut(s) 457
MmeI TCCRAC 2 cut(s) 104, 177
MnlI CCTC 6 cut(s) 54, 102, 139, 142, 160, 169
MroI TCCGGA 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 2 cut(s) 369, 411
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 4 cut(s) 45, 192, 259, 438
MspR9I CCNGG 4 cut(s) 46, 193, 378, 390
MvaI CCWGG 2 cut(s) 378, 390
MwoI GCNNNNNNNGC 3 cut(s) 129, 170, 428
NciI CCSGG 2 cut(s) 46, 193
NdeII GATC 3 cut(s) 9, 76, 237
NlaIII CATG 2 cut(s) 127, 359
NlaIV GGNNCC 2 cut(s) 70, 436
PciSI GCTCTTC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 141
Pfl23II CGTACG 1 cut(s) 84
PfoI TCCNGGA 1 cut(s) 388
PkrI GCNGC 5 cut(s) 94, 97, 131, 228, 290
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 376, 388
PspGI CCWGG 2 cut(s) 376, 388
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 70, 436
PspPI GGNCC 1 cut(s) 111
RsaI GTAC 2 cut(s) 86, 234
RsaNI GTAC 2 cut(s) 85, 233
RseI CAYNNNNRTG 1 cut(s) 371
SapI GCTCTTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 369, 411
SatI GCNGC 5 cut(s) 93, 96, 130, 227, 289
Sau3AI GATC 3 cut(s) 9, 76, 237
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 4 cut(s) 46, 193, 378, 390
SetI ASST 9 cut(s) 36, 86, 171, 208, 293, 345, 433, 460, 500
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 371
Sse9I AATT 3 cut(s) 295, 321, 487
SspMI CTAG 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 44, 191, 376, 388
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 279
TaiI ACGT 1 cut(s) 86
TaqI TCGA 2 cut(s) 75, 144
TasI AATT 3 cut(s) 295, 321, 487
TauI GCSGC 3 cut(s) 98, 132, 229
TfiI GAWTC 1 cut(s) 141
Tru1I TTAA 2 cut(s) 369, 411
Tru9I TTAA 2 cut(s) 369, 411
TseI GCWGC 2 cut(s) 92, 288
TspDTI ATGAA 1 cut(s) 462
TspGWI ACGGA 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 111
VspI ATTAAT 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.