Rh5DG332500

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018005
Physical Location & Seq
Reverse (-)
43858854 .. 43865407
6554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG332500.1

Sequence Viewer

Length: 624 bp
ATGGCTCTGGTACCCTCATCTGCGCCAGTGAACGAGAGCCCAGTGATCACCGAGGTTGACATGGGCGGCGACGACTCCACACCCACCGTCCGAGCCACCGTCGTCCAAGCCTCCACCGTCTTCTACGACACTCCCGCCACTCTAGATAAGGCTGAGAGATTGTTGGCTGAAGCAGCTGGGTATGGATCGCAGCTGGTTGTGTTTCCGGAAGCATTTGTGGGAGGGTATCCGCGAGGCTCCAATTTCGGGGTTGCGATTGGGAACAGAACTGCCAAGGGGAAAGAAGAGTTCCGAAAGTATCATGCCGCGGCCATTACTGTGCCTGGTCCTGAAGTTGACCGATTGGCATCAATGGCTGGAAAATATAAGGTATTCTTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACACTTTATTGTACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCACCGAAAAATAATGCCAACAGCATTAGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCCTGTGTTTGATACTCCAATAGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCACTTTTAAGGACAGCAATGTATGCTAAAGGTTAG

Protein Analysis

207

Amino Acids

22.27

Weight (kDa)

6.74

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 31 - 207 5.3e-44 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018005)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25810 FvH4_3g25810 FvH4_3g25810
pyrus_communis pycom11g14250
rosa_laevigata RLG00000001790 RLG00000005908 RLG00000029485
rosa_roxburghii Rroxscaffold_3G00241990
rosa_samantha Rh1CG027800 Rh5DG332500
rosa_wichuraiana Rw6G020870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 10
AccB7I CCANNNNNTGG 1 cut(s) 573
AccI GTMKAC 1 cut(s) 407
AccII CGCG 2 cut(s) 232, 308
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 5 cut(s) 66, 135, 230, 306, 308
AclWI GGATC 3 cut(s) 193, 513, 526
AcoI YGGCCR 1 cut(s) 309
AcuI CTGAAG 2 cut(s) 189, 351
AfaI GTAC 2 cut(s) 12, 421
AfiI CCNNNNNNNGG 2 cut(s) 246, 573
AjnI CCWGG 2 cut(s) 322, 453
AluBI AGCT 2 cut(s) 176, 193
AluI AGCT 2 cut(s) 176, 193
AlwI GGATC 3 cut(s) 193, 513, 526
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 173, 190, 563
Asp718I GGTACC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 1 cut(s) 326
BamHI GGATCC 1 cut(s) 518
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 112
BbvI GCAGC 3 cut(s) 185, 202, 550
BccI CCATC 1 cut(s) 509
BciT130I CCWGG 2 cut(s) 324, 455
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 1 cut(s) 45
BfaI CTAG 1 cut(s) 143
BfuI GTATCC 1 cut(s) 237
BisI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
BlsI GCNGC 6 cut(s) 68, 175, 192, 307, 310, 565
Bme1390I CCNGG 2 cut(s) 324, 455
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 3 cut(s) 12, 238, 520
BmrFI CCNGG 2 cut(s) 324, 455
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 2 cut(s) 356, 576
BmuI ACTGGG 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 112
BpuEI CTTGAG 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 346
BsaJI CCNNGG 4 cut(s) 51, 273, 306, 454
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 573
Bse1I ACTGG 2 cut(s) 26, 41
Bse3DI GCAATG 1 cut(s) 612
Bse8I GATNNNNATC 1 cut(s) 346
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 2 cut(s) 324, 455
BseDI CCNNGG 4 cut(s) 51, 273, 306, 454
BseJI GATNNNNATC 1 cut(s) 346
BseLI CCNNNNNNNGG 2 cut(s) 246, 573
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 2 cut(s) 26, 41
BseXI GCAGC 3 cut(s) 185, 202, 550
BseYI CCCAGC 1 cut(s) 176
Bsh1236I CGCG 2 cut(s) 232, 308
BshFI GGCC 1 cut(s) 311
BshNI GGYRCC 1 cut(s) 10
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 246, 573
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 41
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 3 cut(s) 45, 185, 518
BspACI CCGC 5 cut(s) 66, 135, 230, 306, 308
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 1 cut(s) 145
BspEI TCCGGA 1 cut(s) 205
BspFNI CGCG 2 cut(s) 232, 308
BspLI GGNNCC 3 cut(s) 12, 238, 520
BspPI GGATC 3 cut(s) 193, 513, 526
BspT107I GGYRCC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 612
BsrI ACTGG 2 cut(s) 26, 41
BssECI CCNNGG 4 cut(s) 51, 273, 306, 454
BssMI GATC 3 cut(s) 45, 185, 518
BssNAI GTATAC 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 324, 455
Bst4CI ACNGT 5 cut(s) 88, 100, 118, 319, 424
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 611
BstDEI CTNAG 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 306
BstFNI CGCG 2 cut(s) 232, 308
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 3 cut(s) 48, 188, 521
BstMBI GATC 3 cut(s) 45, 185, 518
BstMWI GCNNNNNNNGC 3 cut(s) 173, 353, 611
BstNI CCWGG 2 cut(s) 324, 455
BstSCI CCNGG 2 cut(s) 322, 453
BstUI CGCG 2 cut(s) 232, 308
BstV1I GCAGC 3 cut(s) 185, 202, 550
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 518
BstYI RGATCY 1 cut(s) 518
BstZ17I GTATAC 1 cut(s) 408
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 1 cut(s) 311
BtgI CCRYGG 1 cut(s) 306
BtsIMutI CAGTG 2 cut(s) 33, 48
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 326
Cfr42I CCGCGG 1 cut(s) 309
Csp6I GTAC 2 cut(s) 11, 420
CviAII CATG 2 cut(s) 61, 302
CviQI GTAC 2 cut(s) 11, 420
DdeI CTNAG 1 cut(s) 153
DpnI GATC 3 cut(s) 47, 187, 520
DpnII GATC 3 cut(s) 45, 185, 518
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 326
Eco57I CTGAAG 2 cut(s) 189, 351
EcoRII CCWGG 2 cut(s) 322, 453
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 64, 305
FaiI YATR 6 cut(s) 62, 183, 303, 366, 408, 612
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FatI CATG 2 cut(s) 60, 301
FauI CCCGC 1 cut(s) 142
FbaI TGATCA 1 cut(s) 45
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 24
GluI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
GsaI CCCAGC 1 cut(s) 180
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 206
HhaI GCGC 1 cut(s) 25
Hin1II CATG 2 cut(s) 64, 305
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 2 cut(s) 58, 337
HindII GTYRAC 2 cut(s) 58, 337
HinfI GANTC 3 cut(s) 74, 437, 495
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 4 cut(s) 31, 58, 337, 408
Hpy188I TCNGA 2 cut(s) 92, 293
Hpy188III TCNNGA 3 cut(s) 143, 206, 329
Hpy8I GTNNAC 4 cut(s) 31, 58, 337, 408
Hpy99I CGWCG 2 cut(s) 74, 104
HpyAV CCTTC 1 cut(s) 395
HpyCH4III ACNGT 5 cut(s) 88, 100, 118, 319, 424
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 353, 611
HpyF3I CTNAG 1 cut(s) 153
Hsp92II CATG 2 cut(s) 64, 305
HspAI GCGC 1 cut(s) 23
Kpn2I TCCGGA 1 cut(s) 205
KpnI GGTACC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 45
KspI CCGCGG 1 cut(s) 309
Kzo9I GATC 3 cut(s) 45, 185, 518
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 3 cut(s) 185, 202, 550
LweI GCATC 2 cut(s) 356, 576
MaeI CTAG 1 cut(s) 143
MalI GATC 3 cut(s) 47, 187, 520
MboI GATC 3 cut(s) 45, 185, 518
MboII GAAGA 2 cut(s) 112, 296
MflI RGATCY 1 cut(s) 518
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 2 cut(s) 241, 525
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 5 cut(s) 25, 46, 121, 215, 227
MroI TCCGGA 1 cut(s) 205
MseI TTAA 1 cut(s) 596
MslI CAYNNNNRTG 1 cut(s) 317
MspA1I CMGCKG 3 cut(s) 176, 193, 308
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 324, 455
MvaI CCWGG 2 cut(s) 324, 455
MvnI CGCG 2 cut(s) 232, 308
MwoI GCNNNNNNNGC 3 cut(s) 173, 353, 611
NdeII GATC 3 cut(s) 45, 185, 518
NlaIII CATG 2 cut(s) 64, 305
NlaIV GGNNCC 3 cut(s) 12, 238, 520
PfeI GAWTC 2 cut(s) 437, 495
PflMI CCANNNNNTGG 1 cut(s) 573
PkrI GCNGC 6 cut(s) 68, 175, 192, 307, 310, 565
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
Psp6I CCWGG 2 cut(s) 322, 453
PspFI CCCAGC 1 cut(s) 176
PspGI CCWGG 2 cut(s) 322, 453
PspN4I GGNNCC 3 cut(s) 12, 238, 520
PspPI GGNCC 1 cut(s) 326
PsuI RGATCY 1 cut(s) 518
PvuII CAGCTG 2 cut(s) 176, 193
RsaI GTAC 2 cut(s) 12, 421
RsaNI GTAC 2 cut(s) 11, 420
RseI CAYNNNNRTG 1 cut(s) 317
SacII CCGCGG 1 cut(s) 309
SaqAI TTAA 1 cut(s) 596
SatI GCNGC 6 cut(s) 67, 174, 191, 306, 309, 564
Sau3AI GATC 3 cut(s) 45, 185, 518
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 324, 455
SduI GDGCHC 1 cut(s) 41
SetI ASST 8 cut(s) 57, 178, 195, 372, 448, 456, 562, 622
SfaNI GCATC 2 cut(s) 356, 576
Sfr303I CCGCGG 1 cut(s) 309
SgrBI CCGCGG 1 cut(s) 309
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 2 cut(s) 241, 525
SsiI CCGC 5 cut(s) 66, 135, 230, 306, 308
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 322, 453
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 5 cut(s) 88, 100, 118, 319, 424
TaqII GACCGA 1 cut(s) 354
TasI AATT 2 cut(s) 241, 525
TatI WGTACW 1 cut(s) 419
TauI GCSGC 3 cut(s) 69, 308, 311
TfiI GAWTC 2 cut(s) 437, 495
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TscAI CASTG 2 cut(s) 33, 48
TseI GCWGC 3 cut(s) 173, 190, 563
TspRI CASTG 2 cut(s) 33, 48
Van91I CCANNNNNTGG 1 cut(s) 573
VpaK11BI GGWCC 1 cut(s) 326
XbaI TCTAGA 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.