RLG00000003191

Tobamovirus multiplication protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013281
Physical Location & Seq
Forward (+)
45427064 .. 45429727
2664 bp
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UTR
Exon/CDS
Intron
RLM00000003191

Sequence Viewer

Length: 399 bp
ATGGCGAGCTCAACCTCCGGTGGAAGAAGCAGCAGCAGAGAGAATACGGCAAAAAACATGGTCTCCGATCAGATTTCTCAGGCCGTACTATCCACTTCCAACCTCCTCCACCTCATGCAGCAGTCCTCGCCTTCCCAGGCCCAACTGATAAAGCTTCCAAAGAACCTTTTGCTCAAATCATCTACAATTAAGAATACTGGACATTTATTGGAGCAAATGCCTCGAGTCATTTCATCTCTGGATGCTCATATGGAAACTGGGTTGCAAAGTCTGCCTCATCTGCAAACTGTAATCCAACTACTCGCAAATATGGAAAGCTGCCAACTCAGTTCTCTCTCACAAGCTCATGTCCTTCAAGAGGAACATGAGGCAGAAAACCAACCTTCAAATGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0016032 GO:0019058 GO:0019079 GO:0044403 GO:0044419 GO:0046786 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS7

Protein Analysis

133

Amino Acids

14.36

Weight (kDa)

6.04

Isoelectric Point (pI)

60.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32370 AT1G32370 AT1G32370 AT1G32370
fragaria_vesca FvH4_5g21430 FvH4_5g21430
malus_domestica MD06G1029000.v1.1
prunus_persica Prupe.5G042800_v2.0.a1
pyrus_communis pycom06g02310
rosa_chinensis RchiOBHm_Chr7g0208861
rosa_laevigata RLG00000003191
rosa_multiflora Rmu_sc0023015.1_g000007
rosa_roxburghii Rroxscaffold_3G00250190
rosa_rugosa Rorug07G0106700
rosa_samantha Rh7AG241800 Rh7BG236400 Rh7CG256400
rosa_wichuraiana Rw7G020480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 358
AgsI TTSAA 2 cut(s) 356, 387
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 4 cut(s) 9, 154, 318, 344
AluI AGCT 4 cut(s) 9, 154, 318, 344
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 67
Ama87I CYCGRG 1 cut(s) 222
AoxI GGCC 2 cut(s) 81, 138
ApeKI GCWGC 4 cut(s) 30, 33, 118, 318
AspS9I GGNCC 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 222
BanII GRGCYC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 4 cut(s) 42, 45, 130, 305
BceAI ACGGC 2 cut(s) 63, 68
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 67
BisI GCNGC 4 cut(s) 31, 34, 119, 319
BlsI GCNGC 4 cut(s) 32, 35, 120, 320
Bme1390I CCNGG 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 232
BmuI ACTGGG 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse1I ACTGG 2 cut(s) 202, 262
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 358
BseMII CTCAG 2 cut(s) 92, 340
BseNI ACTGG 2 cut(s) 202, 262
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 4 cut(s) 42, 45, 130, 305
BshFI GGCC 2 cut(s) 83, 140
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 358
BsmAI GTCTC 1 cut(s) 67
BsnI GGCC 2 cut(s) 83, 140
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 1 cut(s) 67
BspANI GGCC 2 cut(s) 83, 140
BspCNI CTCAG 2 cut(s) 91, 339
BspTNI GGTCTC 1 cut(s) 67
BsrI ACTGG 2 cut(s) 202, 262
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 289
BstAPI GCANNNNNTGC 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 78, 326
BstENI CCTNNNNNAGG 1 cut(s) 356
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 1 cut(s) 70
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 3 cut(s) 127, 271, 280
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BstV1I GCAGC 4 cut(s) 42, 45, 130, 305
BsuRI GGCC 2 cut(s) 83, 140
BtsCI GGATG 1 cut(s) 247
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 86
CviAII CATG 4 cut(s) 58, 115, 347, 365
CviJI RGCY 6 cut(s) 9, 83, 140, 154, 318, 344
CviKI_1 RGCY 6 cut(s) 9, 83, 140, 154, 318, 344
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 2 cut(s) 78, 326
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
Ecl136II GAGCTC 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 4 cut(s) 61, 118, 350, 368
FaiI YATR 7 cut(s) 59, 116, 249, 251, 311, 348, 366
FatI CATG 4 cut(s) 57, 114, 346, 364
FauNDI CATATG 1 cut(s) 249
Fnu4HI GCNGC 4 cut(s) 31, 34, 119, 319
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 4 cut(s) 31, 34, 119, 319
GluI GCNGC 4 cut(s) 31, 34, 119, 319
HaeIII GGCC 2 cut(s) 83, 140
HapII CCGG 1 cut(s) 18
Hin1II CATG 4 cut(s) 61, 118, 350, 368
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 1 cut(s) 225
HpaII CCGG 1 cut(s) 18
Hpy188I TCNGA 2 cut(s) 67, 72
Hpy188III TCNNGA 2 cut(s) 239, 356
HpyAV CCTTC 3 cut(s) 141, 362, 393
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4V TGCA 3 cut(s) 118, 265, 283
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 271, 280
HpyF3I CTNAG 2 cut(s) 78, 326
Hsp92II CATG 4 cut(s) 61, 118, 350, 368
Kzo9I GATC 1 cut(s) 67
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 7 cut(s) 31, 65, 122, 149, 183, 224, 243
Lsp1109I GCAGC 4 cut(s) 42, 45, 130, 305
LweI GCATC 1 cut(s) 232
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MboII GAAGA 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 1 cut(s) 186
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 2 cut(s) 123, 319
MnlI CCTC 9 cut(s) 25, 113, 116, 122, 136, 231, 285, 352, 361
MseI TTAA 1 cut(s) 189
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 127, 271, 280
NdeI CATATG 1 cut(s) 249
NdeII GATC 1 cut(s) 67
NlaIII CATG 4 cut(s) 61, 118, 350, 368
PaeR7I CTCGAG 1 cut(s) 222
PkrI GCNGC 4 cut(s) 32, 35, 120, 320
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspPI GGNCC 1 cut(s) 139
PspXI VCTCGAGB 1 cut(s) 222
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 31, 34, 119, 319
Sau3AI GATC 1 cut(s) 67
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 1 cut(s) 234
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 1 cut(s) 11
SetI ASST 9 cut(s) 11, 17, 105, 114, 156, 168, 320, 346, 385
SfaNI GCATC 1 cut(s) 232
Sfr274I CTCGAG 1 cut(s) 222
SlaI CTCGAG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
Sse9I AATT 1 cut(s) 186
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 289
TaqI TCGA 1 cut(s) 223
TasI AATT 1 cut(s) 186
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 4 cut(s) 30, 33, 118, 318
TspDTI ATGAA 1 cut(s) 222
XagI CCTNNNNNAGG 1 cut(s) 356
XhoI CTCGAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.