RLG00000003572

14 kDa proline-rich protein DC2.15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015790
Physical Location & Seq
Reverse (-)
50132114 .. 50132458
345 bp
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UTR
Exon/CDS
Intron
RLM00000003572

Sequence Viewer

Length: 345 bp
ATGCTTGTGCCTCATGTCCTGCTCCTATTTCCCAACATCACCCAACTCCAAAACCGAAAACCGGTACCACGCTTCAGCTCCACAGCCGCATGCCCCAGGGATACCCTAAAGTTGGGGGTTTGCGGTAACGTGCTCCGAAGGTTGCTTAATCTTGAAGTTGGTAACCCTCTAGTGAAACCTTGTTGCTCTCTCCTGTATGGGCTTGTTGACCTCGAGGCTGCTGTTTGCCTTTGCACTGCCATCAAAGCCAACGTTTTGGGTGTCAATCTCAACATTCCGGCTGCTCTCAGCCTGCTTCTTAATGTTTGTGGGGACAAGGTTCCGAAAGGATACCAATGGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

115

Amino Acids

12.31

Weight (kDa)

9.14

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrophob_seed PF14547 31 - 112 1.3e-24 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 31 - 110 2.3e-14 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12470 AT4G12510 AT4G12520 AT4G12530 AT4G22460
rosa_chinensis RchiOBHm_Chr7g0202781
rosa_laevigata RLG00000003572
rosa_multiflora Rmu_sc0002590.1_g000021 Rmu_sc0003429.1_g000014
rosa_roxburghii Rroxscaffold_3G00254270
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG200800 Rh7CG212200 Rh7DG207800
rosa_wichuraiana Rw7G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 64
AccB1I GGYRCC 1 cut(s) 64
AciI CCGC 2 cut(s) 87, 123
AclI AACGTT 1 cut(s) 252
AcuI CTGAAG 1 cut(s) 58
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 2 cut(s) 61, 112
AgeI ACCGGT 1 cut(s) 61
AgsI TTSAA 1 cut(s) 155
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 1 cut(s) 78
AluI AGCT 1 cut(s) 78
Alw21I GWGCWC 1 cut(s) 135
Ama87I CYCGRG 1 cut(s) 212
ApeKI GCWGC 2 cut(s) 218, 281
AsiGI ACCGGT 1 cut(s) 61
Asp718I GGTACC 1 cut(s) 64
AspS9I GGNCC 1 cut(s) 339
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 339
BanI GGYRCC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 205, 268
BccI CCATC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 97
BciVI GTATCC 2 cut(s) 94, 323
BfaI CTAG 2 cut(s) 170, 343
BfuI GTATCC 2 cut(s) 94, 323
BisI GCNGC 3 cut(s) 87, 219, 282
BlsI GCNGC 3 cut(s) 88, 220, 283
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 339
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 2 cut(s) 66, 321
BmrFI CCNGG 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 95, 96
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 112
Bse118I RCCGGY 1 cut(s) 61
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 2 cut(s) 95, 96
BseLI CCNNNNNNNGG 2 cut(s) 61, 112
BseMII CTCAG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 205, 268
BshNI GGYRCC 1 cut(s) 64
BshTI ACCGGT 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 62, 278
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 61, 112
BsmFI GGGAC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 135
BspACI CCGC 2 cut(s) 87, 123
BspCNI CTCAG 1 cut(s) 300
BspLI GGNNCC 2 cut(s) 66, 321
BspT107I GGYRCC 1 cut(s) 64
BsrFI RCCGGY 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 95, 96
Bst2UI CCWGG 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 91, 293
BstDEI CTNAG 1 cut(s) 287
BstEII GGTNACC 1 cut(s) 161
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 1 cut(s) 93
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 205, 268
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuI GTATCC 2 cut(s) 94, 323
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 91, 293
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 339
Csp6I GTAC 1 cut(s) 65
CspAI ACCGGT 1 cut(s) 61
CviAII CATG 2 cut(s) 14, 90
CviJI RGCY 7 cut(s) 78, 86, 202, 218, 248, 281, 291
CviKI_1 RGCY 7 cut(s) 78, 86, 202, 218, 248, 281, 291
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 212
Eco91I GGTNACC 1 cut(s) 161
EcoO65I GGTNACC 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 95
FaeI CATG 2 cut(s) 17, 93
FaiI YATR 3 cut(s) 15, 91, 198
FaqI GGGAC 1 cut(s) 326
FatI CATG 2 cut(s) 13, 89
Fnu4HI GCNGC 3 cut(s) 87, 219, 282
Fsp4HI GCNGC 3 cut(s) 87, 219, 282
FspBI CTAG 2 cut(s) 170, 343
GluI GCNGC 3 cut(s) 87, 219, 282
HapII CCGG 2 cut(s) 62, 278
Hin1II CATG 2 cut(s) 17, 93
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HpaII CCGG 2 cut(s) 62, 278
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 137, 324
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 132
HpyCH4IV ACGT 2 cut(s) 129, 252
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 2 cut(s) 129, 252
Hsp92II CATG 2 cut(s) 17, 93
KpnI GGTACC 1 cut(s) 68
LmnI GCTCC 3 cut(s) 27, 83, 138
LpnPI CCDG 7 cut(s) 32, 75, 82, 109, 206, 291, 305
Lsp1109I GCAGC 2 cut(s) 205, 268
MaeI CTAG 2 cut(s) 170, 343
MaeII ACGT 2 cut(s) 129, 252
MaeIII GTNAC 2 cut(s) 125, 161
MhlI GDGCHC 1 cut(s) 135
MnlI CCTC 4 cut(s) 21, 177, 208, 221
MseI TTAA 2 cut(s) 147, 300
MspI CCGG 2 cut(s) 62, 278
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 1 cut(s) 245
NlaIII CATG 2 cut(s) 17, 93
NlaIV GGNNCC 2 cut(s) 66, 321
NspI RCATGY 1 cut(s) 93
PaeI GCATGC 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 212
PasI CCCWGGG 1 cut(s) 96
PinAI ACCGGT 1 cut(s) 61
PkrI GCNGC 3 cut(s) 88, 220, 283
Psp1406I AACGTT 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 95
PspEI GGTNACC 1 cut(s) 161
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 66, 321
PspPI GGNCC 1 cut(s) 339
PspXI VCTCGAGB 1 cut(s) 212
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SaqAI TTAA 2 cut(s) 147, 300
SatI GCNGC 3 cut(s) 87, 219, 282
Sau96I GGNCC 1 cut(s) 339
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 1 cut(s) 135
SetI ASST 8 cut(s) 80, 132, 143, 181, 213, 255, 321, 344
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 339
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SphI GCATGC 1 cut(s) 93
SsiI CCGC 2 cut(s) 87, 123
SspMI CTAG 2 cut(s) 170, 343
StyD4I CCNGG 1 cut(s) 95
TaiI ACGT 2 cut(s) 132, 255
TaqI TCGA 1 cut(s) 213
TauI GCSGC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 147, 300
Tru9I TTAA 2 cut(s) 147, 300
TscAI CASTG 1 cut(s) 241
TseI GCWGC 2 cut(s) 218, 281
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 339
XceI RCATGY 1 cut(s) 93
XhoI CTCGAG 1 cut(s) 212
XspI CTAG 2 cut(s) 170, 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.