Rroxscaffold_3G00254270

14 kDa proline-rich protein DC2.15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015790
Physical Location & Seq
Reverse (-)
48406099 .. 48406539
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00254270.1

Sequence Viewer

Length: 441 bp
ATGTATGTACGGTTTGAGATCAAGATGGGTTCCAAAAACAATGCTTCAGTTGCTCTGTTTATCTCAGCACTTAATCTCCTTATTTTTGCCCGGGTCAATGCTTGTGCCTCATGTCCTGCTCCTATTTCCCAACACCACCCAACTCCAAACCGAAAACCGGTACCACGCTTCAGCTTCACAGCCGCATGCCCCAGGGATACCCTAAAGTTAGGGGTTTGCGGTAACGTGCTCCGAAGGTTGCTTAATCTTGAAGCTGGTAACCCTCTGGTGAAACTTTGTTGCTCTCTCCTGTATGGCCATGTTGACCTCGAGGCTGCTGTTTGCCTTTGCACTGCCATCAAAGCCAACGTTTTGGGTGTCAATCTCAACATTCCGGCTGCTCTCAGCCTGCTTCTTAATGTTTGTGGGGACAAGGTTCCGAAAGGATACCAATGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

146

Amino Acids

15.7

Weight (kDa)

9.32

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrophob_seed PF14547 63 - 144 4.2e-22 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 63 - 142 1.3e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12470 AT4G12510 AT4G12520 AT4G12530 AT4G22460
rosa_chinensis RchiOBHm_Chr7g0202781
rosa_laevigata RLG00000003572
rosa_multiflora Rmu_sc0002590.1_g000021 Rmu_sc0003429.1_g000014
rosa_roxburghii Rroxscaffold_3G00254270
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG200800 Rh7CG212200 Rh7DG207800
rosa_wichuraiana Rw7G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 308
Acc65I GGTACC 1 cut(s) 160
AccB1I GGYRCC 1 cut(s) 160
AciI CCGC 2 cut(s) 183, 219
AclI AACGTT 1 cut(s) 348
AcoI YGGCCR 1 cut(s) 295
AcuI CTGAAG 2 cut(s) 30, 154
AfaI GTAC 2 cut(s) 9, 162
AfiI CCNNNNNNNGG 1 cut(s) 157
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 1 cut(s) 251
AjnI CCWGG 1 cut(s) 191
AluBI AGCT 2 cut(s) 174, 254
AluI AGCT 2 cut(s) 174, 254
Alw21I GWGCWC 1 cut(s) 231
Ama87I CYCGRG 2 cut(s) 90, 308
AoxI GGCC 2 cut(s) 295, 435
ApeKI GCWGC 2 cut(s) 314, 377
AsiGI ACCGGT 1 cut(s) 157
Asp718I GGTACC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 435
AsuC2I CCSGG 2 cut(s) 91, 92
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 2 cut(s) 90, 308
BalI TGGCCA 1 cut(s) 297
BanI GGYRCC 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 301, 364
BccI CCATC 2 cut(s) 19, 344
BciT130I CCWGG 1 cut(s) 193
BciVI GTATCC 2 cut(s) 190, 419
BcnI CCSGG 2 cut(s) 91, 92
BfaI CTAG 1 cut(s) 439
BfuI GTATCC 2 cut(s) 190, 419
BisI GCNGC 3 cut(s) 183, 315, 378
BlsI GCNGC 3 cut(s) 184, 316, 379
Bme1390I CCNGG 3 cut(s) 91, 92, 193
BmeT110I CYCGRG 2 cut(s) 90, 308
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 3 cut(s) 31, 162, 417
BmrFI CCNGG 3 cut(s) 91, 92, 193
BpuMI CCSGG 2 cut(s) 91, 92
BsaJI CCNNGG 3 cut(s) 90, 191, 192
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse118I RCCGGY 1 cut(s) 157
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 3 cut(s) 90, 191, 192
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 78, 397
BseXI GCAGC 2 cut(s) 301, 364
BshFI GGCC 2 cut(s) 297, 437
BshNI GGYRCC 1 cut(s) 160
BshTI ACCGGT 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 2 cut(s) 90, 308
BsiSI CCGG 3 cut(s) 91, 158, 374
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 422
BsnI GGCC 2 cut(s) 297, 437
BsoBI CYCGRG 2 cut(s) 90, 308
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 1 cut(s) 18
BspACI CCGC 2 cut(s) 183, 219
BspANI GGCC 2 cut(s) 297, 437
BspCNI CTCAG 2 cut(s) 77, 396
BspLI GGNNCC 3 cut(s) 31, 162, 417
BspT107I GGYRCC 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 157
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 3 cut(s) 90, 191, 192
BssMI GATC 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 187, 389
BstDEI CTNAG 2 cut(s) 64, 383
BstEII GGTNACC 1 cut(s) 257
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 2 cut(s) 50, 341
BstNI CCWGG 1 cut(s) 193
BstNSI RCATGY 1 cut(s) 189
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 3 cut(s) 89, 90, 191
BstV1I GCAGC 2 cut(s) 301, 364
BstXI CCANNNNNNTGG 1 cut(s) 352
BsuI GTATCC 2 cut(s) 190, 419
BsuRI GGCC 2 cut(s) 297, 437
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 2 cut(s) 187, 389
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 435
Cfr9I CCCGGG 1 cut(s) 90
Csp6I GTAC 2 cut(s) 8, 161
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 3 cut(s) 111, 186, 299
CviJI RGCY 9 cut(s) 174, 182, 254, 297, 314, 344, 377, 387, 437
CviKI_1 RGCY 9 cut(s) 174, 182, 254, 297, 314, 344, 377, 387, 437
CviQI GTAC 2 cut(s) 8, 161
DdeI CTNAG 2 cut(s) 64, 383
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 30, 154
Eco88I CYCGRG 2 cut(s) 90, 308
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 191
FaeI CATG 3 cut(s) 114, 189, 302
FaiI YATR 5 cut(s) 6, 112, 187, 294, 300
FaqI GGGAC 1 cut(s) 422
FatI CATG 3 cut(s) 110, 185, 298
Fnu4HI GCNGC 3 cut(s) 183, 315, 378
Fsp4HI GCNGC 3 cut(s) 183, 315, 378
FspBI CTAG 1 cut(s) 439
GluI GCNGC 3 cut(s) 183, 315, 378
HaeIII GGCC 2 cut(s) 297, 437
HapII CCGG 3 cut(s) 91, 158, 374
Hin1II CATG 3 cut(s) 114, 189, 302
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HpaII CCGG 3 cut(s) 91, 158, 374
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 1 cut(s) 304
Hpy188I TCNGA 2 cut(s) 233, 420
Hpy188III TCNNGA 2 cut(s) 22, 248
Hpy8I GTNNAC 1 cut(s) 304
HpyAV CCTTC 1 cut(s) 228
HpyCH4III ACNGT 1 cut(s) 12
HpyCH4IV ACGT 2 cut(s) 225, 348
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 341
HpyF3I CTNAG 2 cut(s) 64, 383
HpySE526I ACGT 2 cut(s) 225, 348
Hsp92II CATG 3 cut(s) 114, 189, 302
KpnI GGTACC 1 cut(s) 164
Kzo9I GATC 1 cut(s) 18
LmnI GCTCC 2 cut(s) 124, 234
Lsp1109I GCAGC 2 cut(s) 301, 364
MaeI CTAG 1 cut(s) 439
MaeII ACGT 2 cut(s) 225, 348
MaeIII GTNAC 2 cut(s) 221, 257
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 297
MluNI TGGCCA 1 cut(s) 297
MnlI CCTC 4 cut(s) 118, 273, 304, 317
Mox20I TGGCCA 1 cut(s) 297
MscI TGGCCA 1 cut(s) 297
MseI TTAA 3 cut(s) 72, 243, 396
Msp20I TGGCCA 1 cut(s) 297
MspI CCGG 3 cut(s) 91, 158, 374
MspR9I CCNGG 3 cut(s) 91, 92, 193
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 2 cut(s) 50, 341
NciI CCSGG 2 cut(s) 91, 92
NdeII GATC 1 cut(s) 18
NlaIII CATG 3 cut(s) 114, 189, 302
NlaIV GGNNCC 3 cut(s) 31, 162, 417
NspI RCATGY 1 cut(s) 189
PaeI GCATGC 1 cut(s) 189
PaeR7I CTCGAG 1 cut(s) 308
PasI CCCWGGG 1 cut(s) 192
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 3 cut(s) 184, 316, 379
Psp1406I AACGTT 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 191
PspEI GGTNACC 1 cut(s) 257
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 3 cut(s) 31, 162, 417
PspPI GGNCC 1 cut(s) 435
PspXI VCTCGAGB 1 cut(s) 308
RsaI GTAC 2 cut(s) 9, 162
RsaNI GTAC 2 cut(s) 8, 161
SaqAI TTAA 3 cut(s) 72, 243, 396
SatI GCNGC 3 cut(s) 183, 315, 378
Sau3AI GATC 1 cut(s) 18
Sau96I GGNCC 1 cut(s) 435
ScrFI CCNGG 3 cut(s) 91, 92, 193
SduI GDGCHC 1 cut(s) 231
SetI ASST 7 cut(s) 176, 228, 239, 256, 309, 351, 417
Sfr274I CTCGAG 1 cut(s) 308
SlaI CTCGAG 1 cut(s) 308
SmaI CCCGGG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SphI GCATGC 1 cut(s) 189
SsiI CCGC 2 cut(s) 183, 219
SspMI CTAG 1 cut(s) 439
StyD4I CCNGG 3 cut(s) 89, 90, 191
TaaI ACNGT 1 cut(s) 12
TaiI ACGT 2 cut(s) 228, 351
TaqI TCGA 1 cut(s) 309
TauI GCSGC 1 cut(s) 185
Tru1I TTAA 3 cut(s) 72, 243, 396
Tru9I TTAA 3 cut(s) 72, 243, 396
TscAI CASTG 1 cut(s) 337
TseI GCWGC 2 cut(s) 314, 377
TspMI CCCGGG 1 cut(s) 90
TspRI CASTG 1 cut(s) 337
XceI RCATGY 1 cut(s) 189
XhoI CTCGAG 1 cut(s) 308
XmaI CCCGGG 1 cut(s) 90
XspI CTAG 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.