Rw7G017560

14 kDa proline-rich protein DC2.15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015790
Physical Location & Seq
Forward (+)
18570164 .. 18570586
423 bp
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UTR
Exon/CDS
Intron
Rw7G017560.1

Sequence Viewer

Length: 423 bp
ATGGGTTCCAAAAACACTGCCTCAGTTGCTCTGTTTATCTCAGCACTCAATCTCCTTATTTTTGCCCTGGTCAATGCTTGTGCCTCATGTCCTGCTCCTATTTCCCAACATCACCCAACTCCAAACCGAAAACCGGTACCACGCTTCACCTCCACAGCCGCATGCCCCAGGGATACCCTAAAGTTAGGGGTCTGCGGTAACTTGCTCCGAAGGTTGATTAATCTTGAAGTTGGTAACCCTCTGGTGAAACCTTGTTGCTCTCTCCTGTATGGCCTTGTTGACCTCGAGGCTGCTGTTTGCCTTTGCACTGCCATCGAAGCCAACGTTTTGGGTGTCAATCTCAACATTCCGGCTGCTCTCAGCCTGCTTCTTAATGTTTGTGGGAACAAAGGTTCCGAAAGGATACCAATGGGCCTAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

140

Amino Acids

14.62

Weight (kDa)

8.83

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrophob_seed PF14547 55 - 130 1.9e-20 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 55 - 129 1.2e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12470 AT4G12510 AT4G12520 AT4G12530 AT4G22460
rosa_chinensis RchiOBHm_Chr7g0202781
rosa_laevigata RLG00000003572
rosa_multiflora Rmu_sc0002590.1_g000021 Rmu_sc0003429.1_g000014
rosa_roxburghii Rroxscaffold_3G00254270
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG200800 Rh7CG212200 Rh7DG207800
rosa_wichuraiana Rw7G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 284
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 1 cut(s) 136
AciI CCGC 2 cut(s) 159, 195
AclI AACGTT 1 cut(s) 324
AfaI GTAC 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 133
AgeI ACCGGT 1 cut(s) 133
AgsI TTSAA 1 cut(s) 227
AjnI CCWGG 2 cut(s) 66, 167
AloI GAACNNNNNNTCC 4 cut(s) 376, 377, 408, 409
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 2 cut(s) 271, 412
ApeKI GCWGC 2 cut(s) 290, 353
AseI ATTAAT 1 cut(s) 219
AsiGI ACCGGT 1 cut(s) 133
Asp718I GGTACC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 412
AsuHPI GGTGA 3 cut(s) 104, 139, 256
AvaI CYCGRG 1 cut(s) 284
BanI GGYRCC 1 cut(s) 136
BbvI GCAGC 2 cut(s) 277, 340
BccI CCATC 1 cut(s) 320
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 2 cut(s) 68, 169
BciVI GTATCC 2 cut(s) 166, 396
BfaI CTAG 1 cut(s) 416
BfuI GTATCC 2 cut(s) 166, 396
BisI GCNGC 3 cut(s) 159, 291, 354
BlsI GCNGC 3 cut(s) 160, 292, 355
Bme1390I CCNGG 2 cut(s) 68, 169
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 3 cut(s) 7, 138, 394
BmrFI CCNGG 2 cut(s) 68, 169
BsaJI CCNNGG 3 cut(s) 66, 167, 168
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse118I RCCGGY 1 cut(s) 133
BseBI CCWGG 2 cut(s) 68, 169
BseDI CCNNGG 3 cut(s) 66, 167, 168
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 3 cut(s) 36, 54, 373
BseXI GCAGC 2 cut(s) 277, 340
BshFI GGCC 2 cut(s) 273, 414
BshNI GGYRCC 1 cut(s) 136
BshTI ACCGGT 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 2 cut(s) 134, 350
BslI CCNNNNNNNGG 1 cut(s) 133
BsnI GGCC 2 cut(s) 273, 414
BsoBI CYCGRG 1 cut(s) 284
BspACI CCGC 2 cut(s) 159, 195
BspANI GGCC 2 cut(s) 273, 414
BspCNI CTCAG 3 cut(s) 35, 53, 372
BspLI GGNNCC 3 cut(s) 7, 138, 394
BspT107I GGYRCC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 133
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 3 cut(s) 66, 167, 168
Bst2UI CCWGG 2 cut(s) 68, 169
BstC8I GCNNGC 2 cut(s) 163, 365
BstDEI CTNAG 3 cut(s) 22, 40, 359
BstEII GGTNACC 1 cut(s) 233
BstMWI GCNNNNNNNGC 2 cut(s) 26, 317
BstNI CCWGG 2 cut(s) 68, 169
BstNSI RCATGY 1 cut(s) 165
BstPI GGTNACC 1 cut(s) 233
BstSCI CCNGG 2 cut(s) 66, 167
BstV1I GCAGC 2 cut(s) 277, 340
BstXI CCANNNNNNTGG 1 cut(s) 328
BsuI GTATCC 2 cut(s) 166, 396
BsuRI GGCC 2 cut(s) 273, 414
BtsI GCAGTG 2 cut(s) 15, 306
BtsIMutI CAGTG 2 cut(s) 15, 306
Cac8I GCNNGC 2 cut(s) 163, 365
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 412
Csp6I GTAC 1 cut(s) 137
CspAI ACCGGT 1 cut(s) 133
CviAII CATG 2 cut(s) 87, 162
CviJI RGCY 8 cut(s) 158, 273, 290, 320, 353, 363, 414, 419
CviKI_1 RGCY 8 cut(s) 158, 273, 290, 320, 353, 363, 414, 419
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 3 cut(s) 22, 40, 359
Eco88I CYCGRG 1 cut(s) 284
Eco91I GGTNACC 1 cut(s) 233
EcoO65I GGTNACC 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 66, 167
FaeI CATG 2 cut(s) 90, 165
FaiI YATR 3 cut(s) 88, 163, 270
FatI CATG 2 cut(s) 86, 161
Fnu4HI GCNGC 3 cut(s) 159, 291, 354
Fsp4HI GCNGC 3 cut(s) 159, 291, 354
FspBI CTAG 1 cut(s) 416
GluI GCNGC 3 cut(s) 159, 291, 354
HaeIII GGCC 2 cut(s) 273, 414
HapII CCGG 2 cut(s) 134, 350
Hin1II CATG 2 cut(s) 90, 165
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HpaII CCGG 2 cut(s) 134, 350
HphI GGTGA 3 cut(s) 104, 139, 256
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 2 cut(s) 209, 397
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 204
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 1 cut(s) 306
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 317
HpyF3I CTNAG 3 cut(s) 22, 40, 359
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 2 cut(s) 90, 165
KpnI GGTACC 1 cut(s) 140
LmnI GCTCC 2 cut(s) 100, 210
Lsp1109I GCAGC 2 cut(s) 277, 340
MaeI CTAG 1 cut(s) 416
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 197, 233
MnlI CCTC 6 cut(s) 31, 94, 160, 249, 280, 293
MseI TTAA 2 cut(s) 219, 372
MspI CCGG 2 cut(s) 134, 350
MspR9I CCNGG 2 cut(s) 68, 169
MvaI CCWGG 2 cut(s) 68, 169
MwoI GCNNNNNNNGC 2 cut(s) 26, 317
NlaIII CATG 2 cut(s) 90, 165
NlaIV GGNNCC 3 cut(s) 7, 138, 394
NspI RCATGY 1 cut(s) 165
PaeI GCATGC 1 cut(s) 165
PaeR7I CTCGAG 1 cut(s) 284
PasI CCCWGGG 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PinAI ACCGGT 1 cut(s) 133
PkrI GCNGC 3 cut(s) 160, 292, 355
PshBI ATTAAT 1 cut(s) 219
Psp1406I AACGTT 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 66, 167
PspEI GGTNACC 1 cut(s) 233
PspGI CCWGG 2 cut(s) 66, 167
PspN4I GGNNCC 3 cut(s) 7, 138, 394
PspPI GGNCC 1 cut(s) 412
PspXI VCTCGAGB 1 cut(s) 284
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
SaqAI TTAA 2 cut(s) 219, 372
SatI GCNGC 3 cut(s) 159, 291, 354
Sau96I GGNCC 1 cut(s) 412
ScrFI CCNGG 2 cut(s) 68, 169
SetI ASST 6 cut(s) 152, 215, 253, 285, 327, 394
Sfr274I CTCGAG 1 cut(s) 284
SlaI CTCGAG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SphI GCATGC 1 cut(s) 165
SsiI CCGC 2 cut(s) 159, 195
SspMI CTAG 1 cut(s) 416
StyD4I CCNGG 2 cut(s) 66, 167
TaiI ACGT 1 cut(s) 327
TaqI TCGA 2 cut(s) 285, 315
TauI GCSGC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 219, 372
Tru9I TTAA 2 cut(s) 219, 372
TscAI CASTG 2 cut(s) 22, 313
TseI GCWGC 2 cut(s) 290, 353
TspRI CASTG 2 cut(s) 22, 313
VspI ATTAAT 1 cut(s) 219
XceI RCATGY 1 cut(s) 165
XhoI CTCGAG 1 cut(s) 284
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.