RLG00000003622

NADH dehydrogenase (ubiquinone) complex I, assembly factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015411
Physical Location & Seq
Reverse (-)
50880038 .. 50881097
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003622

Sequence Viewer

Length: 756 bp
ATGCGGAAGGCTGCATTTGCAGTCCGTGCCTTGAATGTTGAAACAGCTCGTGCAATGGATGTTGCTTCTGATCCCAGGATTGGTCTTATGCGTCTTGTCTGGTGGCAAGAAGCCATAGACAAAATCTATGCCAACAAGTCGATTGAGCACCCAACAGCACAAGCCCTCTTGTCAGTCGTACATGAGAACAAAATTAGCAAGGCATGGTTGAAACGGTCTGTAGAAGCTCGCATCAATGATGCAAGAAAGGAGATTGGTGACGCCCCAGAAAAAATCGAAGACTTGGAAAAATATGCTGAAGACACCATGTCCACTAATCTTTACATGACACTACAAGCTGGTGGTATCAGGTCCACGGCAGCCGATCATGCGGCATCACATATTGGAAAAGCAAGTGGGCTTCTTCTGCTAATTAAGTCACTGCCATACCATGCTAACCGGAACCGCCACTTTTCTTATATCCCAACTGAAGTGGCAGCCAAGCATGGATTGTTGGTAAAACACGGCGGTCAAGCAGAGATCCTTTTAGACTCTCGTGAGGGCCTAAGTGAAGCTGTTTTTGAGATGGCGTCAGTGGCTAATGTCCATTTGCAGAAGGCTCGTGATTTAGCCGGTGCAGTACCTGCAGAAGCTCATCCAGTGCTGCTGCCAGCAGTGCCTGCCCAAGTTCTACTGGACTCCCTGAGGCGGGTGAATTTTGATGTTTTCGACCCAAGGTTAGCACGAGGGATCCTAGTCTTTCCTGCAGTTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
SQS_PSY

Protein Analysis

252

Amino Acids

27.5

Weight (kDa)

8.76

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 1 - 240 1.1e-47 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62730 AT1G62730
fragaria_vesca FvH4_5g00371
malus_domestica MD04G1082000.v1.1
prunus_persica Prupe.5G076400_v2.0.a1
pyrus_communis pycom04g07390
rosa_chinensis RchiOBHm_Chr7g0202141
rosa_laevigata RLG00000003622
rosa_multiflora Rmu_co8054622.1_g000001
rosa_roxburghii Rroxscaffold_3G00254810
rosa_rugosa Rorug07G0068400
rosa_samantha Rh7AG195800 Rh7BG197100 Rh7CG206900 Rh7DG202400
rosa_wichuraiana Rw7G017150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 631
AciI CCGC 5 cut(s) 4, 371, 445, 507, 688
AclWI GGATC 4 cut(s) 65, 514, 724, 737
AcsI RAATTY 1 cut(s) 694
AcuI CTGAAG 2 cut(s) 318, 489
AcyI GRCGYC 2 cut(s) 261, 569
AfaI GTAC 2 cut(s) 180, 621
AfiI CCNNNNNNNGG 4 cut(s) 80, 687, 688, 749
AgsI TTSAA 3 cut(s) 34, 41, 211
AhdI GACNNNNNGTC 1 cut(s) 307
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 5 cut(s) 47, 227, 338, 554, 632
AluI AGCT 5 cut(s) 47, 227, 338, 554, 632
Alw21I GWGCWC 1 cut(s) 150
AlwI GGATC 4 cut(s) 65, 514, 724, 737
AlwNI CAGNNNCTG 2 cut(s) 623, 659
AoxI GGCC 1 cut(s) 541
ApeKI GCWGC 5 cut(s) 11, 359, 476, 643, 646
ApoI RAATTY 1 cut(s) 694
AspS9I GGNCC 2 cut(s) 351, 541
AsuHPI GGTGA 2 cut(s) 269, 703
AvaII GGWCC 1 cut(s) 351
AxyI CCTNAGG 1 cut(s) 683
BamHI GGATCC 1 cut(s) 729
BauI CACGAG 4 cut(s) 48, 534, 600, 723
BbsI GAAGAC 2 cut(s) 285, 306
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 4 cut(s) 371, 488, 630, 633
BccI CCATC 1 cut(s) 559
BceAI ACGGC 2 cut(s) 372, 520
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 1 cut(s) 76
BfaI CTAG 2 cut(s) 734, 754
BfmI CTRYAG 3 cut(s) 219, 624, 744
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 6 cut(s) 12, 360, 372, 477, 644, 647
BlsI GCNGC 6 cut(s) 13, 361, 373, 478, 645, 648
Bme1390I CCNGG 1 cut(s) 76
Bme18I GGWCC 1 cut(s) 351
BmeRI GACNNNNNGTC 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 351, 541
BmiI GGNNCC 2 cut(s) 443, 731
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 3 cut(s) 229, 240, 383
BpiI GAAGAC 2 cut(s) 285, 306
BsaHI GRCGYC 2 cut(s) 261, 569
BsaJI CCNNGG 3 cut(s) 74, 354, 713
BsaWI WCCGGW 1 cut(s) 438
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 687, 688, 749
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 2 cut(s) 638, 678
Bse21I CCTNAGG 1 cut(s) 683
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 76
BseDI CCNNGG 3 cut(s) 74, 354, 713
BseGI GGATG 2 cut(s) 64, 634
BseLI CCNNNNNNNGG 4 cut(s) 80, 687, 688, 749
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 674
BseNI ACTGG 2 cut(s) 638, 678
BseXI GCAGC 4 cut(s) 371, 488, 630, 633
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 2 cut(s) 439, 612
BslI CCNNNNNNNGG 4 cut(s) 80, 687, 688, 749
BsnI GGCC 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 70, 364, 519, 729
BspACI CCGC 5 cut(s) 4, 371, 445, 507, 688
BspANI GGCC 1 cut(s) 543
BspCNI CTCAG 1 cut(s) 675
BspLI GGNNCC 2 cut(s) 443, 731
BspMAI CTGCAG 2 cut(s) 628, 748
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 4 cut(s) 65, 514, 724, 737
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 2 cut(s) 638, 678
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 3 cut(s) 74, 354, 713
BssMI GATC 4 cut(s) 70, 364, 519, 729
BssNI GRCGYC 2 cut(s) 261, 569
BssSI CACGAG 4 cut(s) 48, 534, 600, 723
BssT1I CCWWGG 1 cut(s) 713
Bst2BI CACGAG 4 cut(s) 48, 534, 600, 723
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 1 cut(s) 216
BstACI GRCGYC 2 cut(s) 261, 569
BstAPI GCANNNNNTGC 3 cut(s) 26, 623, 659
BstC8I GCNNGC 3 cut(s) 229, 651, 660
BstDEI CTNAG 2 cut(s) 545, 683
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 64, 634
BstKTI GATC 4 cut(s) 73, 367, 522, 732
BstMBI GATC 4 cut(s) 70, 364, 519, 729
BstMWI GCNNNNNNNGC 8 cut(s) 17, 26, 368, 406, 575, 623, 655, 659
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 3 cut(s) 219, 624, 744
BstV1I GCAGC 4 cut(s) 371, 488, 630, 633
BstV2I GAAGAC 2 cut(s) 285, 306
BstX2I RGATCY 2 cut(s) 519, 729
BstYI RGATCY 2 cut(s) 519, 729
Bsu36I CCTNAGG 1 cut(s) 683
BsuRI GGCC 1 cut(s) 543
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 2 cut(s) 64, 634
BtsI GCAGTG 2 cut(s) 419, 660
BtsIMutI CAGTG 4 cut(s) 419, 579, 645, 660
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 3 cut(s) 229, 651, 660
CaiI CAGNNNCTG 2 cut(s) 623, 659
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 2 cut(s) 351, 541
CseI GACGC 3 cut(s) 80, 269, 558
Csp6I GTAC 2 cut(s) 179, 620
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 7 cut(s) 182, 204, 307, 325, 368, 431, 485
CviQI GTAC 2 cut(s) 179, 620
DdeI CTNAG 2 cut(s) 545, 683
DpnI GATC 4 cut(s) 72, 366, 521, 731
DpnII GATC 4 cut(s) 70, 364, 519, 729
DriI GACNNNNNGTC 1 cut(s) 307
Eam1105I GACNNNNNGTC 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 713
Eco47I GGWCC 1 cut(s) 351
Eco57I CTGAAG 2 cut(s) 318, 489
Eco81I CCTNAGG 1 cut(s) 683
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 713
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 7 cut(s) 185, 207, 310, 328, 371, 434, 488
FatI CATG 7 cut(s) 181, 203, 306, 324, 367, 430, 484
FauI CCCGC 1 cut(s) 681
Fnu4HI GCNGC 6 cut(s) 12, 360, 372, 477, 644, 647
FokI GGATG 2 cut(s) 71, 621
Fsp4HI GCNGC 6 cut(s) 12, 360, 372, 477, 644, 647
FspBI CTAG 2 cut(s) 734, 754
GluI GCNGC 6 cut(s) 12, 360, 372, 477, 644, 647
HaeIII GGCC 1 cut(s) 543
HapII CCGG 2 cut(s) 439, 612
HgaI GACGC 3 cut(s) 80, 269, 558
Hin1I GRCGYC 2 cut(s) 261, 569
Hin1II CATG 7 cut(s) 185, 207, 310, 328, 371, 434, 488
HinfI GANTC 2 cut(s) 530, 677
HpaII CCGG 2 cut(s) 439, 612
HphI GGTGA 2 cut(s) 269, 703
Hpy166II GTNNAC 2 cut(s) 312, 354
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 2 cut(s) 536, 602
Hpy8I GTNNAC 2 cut(s) 312, 354
HpyAV CCTTC 1 cut(s) 589
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4V TGCA 8 cut(s) 14, 20, 53, 242, 592, 617, 626, 746
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 26, 368, 406, 575, 623, 655, 659
HpyF3I CTNAG 2 cut(s) 545, 683
Hsp92I GRCGYC 2 cut(s) 261, 569
Hsp92II CATG 7 cut(s) 185, 207, 310, 328, 371, 434, 488
Kzo9I GATC 4 cut(s) 70, 364, 519, 729
Lsp1109I GCAGC 4 cut(s) 371, 488, 630, 633
LweI GCATC 3 cut(s) 229, 240, 383
MaeI CTAG 2 cut(s) 734, 754
MaeIII GTNAC 2 cut(s) 257, 417
MalI GATC 4 cut(s) 72, 366, 521, 731
MboI GATC 4 cut(s) 70, 364, 519, 729
MboII GAAGA 3 cut(s) 290, 311, 395
MflI RGATCY 2 cut(s) 519, 729
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 3 cut(s) 192, 411, 694
MlyI GAGTC 2 cut(s) 524, 671
MnlI CCTC 4 cut(s) 176, 532, 678, 719
MseI TTAA 1 cut(s) 414
MspI CCGG 2 cut(s) 439, 612
MspR9I CCNGG 1 cut(s) 76
MvaI CCWGG 1 cut(s) 76
MwoI GCNNNNNNNGC 8 cut(s) 17, 26, 368, 406, 575, 623, 655, 659
NdeII GATC 4 cut(s) 70, 364, 519, 729
NlaIII CATG 7 cut(s) 185, 207, 310, 328, 371, 434, 488
NlaIV GGNNCC 2 cut(s) 443, 731
NmuCI GTSAC 2 cut(s) 257, 417
PkrI GCNGC 6 cut(s) 13, 361, 373, 478, 645, 648
PleI GAGTC 2 cut(s) 524, 671
PpsI GAGTC 2 cut(s) 524, 671
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 2 cut(s) 443, 731
PspPI GGNCC 2 cut(s) 351, 541
PstI CTGCAG 2 cut(s) 628, 748
PstNI CAGNNNCTG 2 cut(s) 623, 659
PsuI RGATCY 2 cut(s) 519, 729
RsaI GTAC 2 cut(s) 180, 621
RsaNI GTAC 2 cut(s) 179, 620
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 6 cut(s) 12, 360, 372, 477, 644, 647
Sau3AI GATC 4 cut(s) 70, 364, 519, 729
Sau96I GGNCC 2 cut(s) 351, 541
SchI GAGTC 2 cut(s) 524, 671
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 150
SetI ASST 8 cut(s) 49, 229, 340, 353, 556, 625, 634, 719
SfaNI GCATC 3 cut(s) 229, 240, 383
SfcI CTRYAG 3 cut(s) 219, 624, 744
SinI GGWCC 1 cut(s) 351
Sse9I AATT 3 cut(s) 192, 411, 694
SsiI CCGC 5 cut(s) 4, 371, 445, 507, 688
SspMI CTAG 2 cut(s) 734, 754
StyD4I CCNGG 1 cut(s) 74
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 1 cut(s) 216
TaqI TCGA 3 cut(s) 140, 276, 708
TasI AATT 3 cut(s) 192, 411, 694
TauI GCSGC 1 cut(s) 374
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 4 cut(s) 426, 579, 645, 660
TseFI GTSAC 2 cut(s) 257, 417
TseI GCWGC 5 cut(s) 11, 359, 476, 643, 646
Tsp45I GTSAC 2 cut(s) 257, 417
TspGWI ACGGA 1 cut(s) 14
TspRI CASTG 4 cut(s) 426, 579, 645, 660
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 1 cut(s) 694
XspI CTAG 2 cut(s) 734, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.