RLG00000003977

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013310
Physical Location & Seq
Forward (+)
54934838 .. 54938967
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003977

Sequence Viewer

Length: 1875 bp
ATGGCAACAGCCACATCTACTAGTACTACTACAGCTCTCAAAGCTCACCCCCATCAACAACCACCCACCAAACCCAGACGCCGCAACAACAACAACAACAACAACAACCCGACCCGGAAGCTCGACCCGCCGACGATTGTCTCCCCCGAGAACTCCTGGTGCTGTCCTGCCTCAAAACCAATCCCGACCCATTCCCGCCGCGGCGTTGACCCGACCGGCGAGCTCGACCCGGTTGAGGACTACCCATCTCCGAATTCTTCACCGTCGTCGTCTTTCAGGATCCGGTTCTCTCCGGGGAGCTTATCCCCAGTCATGGACTTCACTCCGGCTTTGAGCAACGGTCACAACGATTCGTATAACTCGAGCTTCACTCGGTTCAATAGTATGCTCAATGCGGGTCTGCTGAACCCAATGTCGCCGCCGCCGCCGCGGTCCAGCCCGACCCTTTTCGAAATGATGGCCAGCGAGCCCGAAATGGCCAACCCGAAACCCGCCCAGATCCACCAAAACGGCGTCGCGCCGCCGCCGCCGCCCCCCAGGACTACCCACATGGGCTTGGTCCAGGACAAGCAGGCGCTGATGATGCAGCGGGTTTCGGACCTCTTGGGTTCCCGGAGCCCCGGGAACCAGTTCAATGACCCGACCAACAGTGACATCAAGCTGACGTTGAGCGCCAAGGACGGGTTCAGCGTCTCGATGAGTCTGCACCGTCCGATTCTCATCGCGCACAGCCGGTTTTTCGCCGTGAAGCTCTCGGACAGGTGGGTCAAGCAGCAGAGAACGACGCCGTCTTCGTCGCCGCCGTACAATGTGGAGATTGCTGACTGTGATGACGTGGAGGTTTATATTGAGGCTTTGAGGTTGATGTATTGTAAGGATCTGAGGAAGAGGCTAATGAAGGAGGACGTTCCTAAAGTGCTTGGGATTTTGAAGGTTTCGGCGGCAATTGGGTTTGATGCTGGTGTTTTATCTTGTTTGGAGTACTTGGAGGCGGCGCCGTGGGCTGAGGAAGAAGAGGAGAAGGTGGCATCGCTCTTGTCGGAGCTGCGGCTTGAAGGTGTTGGAGCTGGGGAAGTGTTGAAGAGGGTGTCGTTGGAAGTGGCTAATGGGACTGAGGAGGGTAATGACAATGAAGAAGTTTTAGTAAAGCTTCTGCATGTGGTTCTTGAAGGAAAAGATGAGAAGGCAAGACGCGAGATGAAAGGATTGGTGACTAAGATGCTTCGTGAGAATTCGTCTCATAATGAACTCCGGAAAGAGTCCTTGTACTCGGCTTGTGATGGGTGTTTGCAATTGCTTTGCCACCATTTTTCTCGGGCAGCAGAGGCAGACTTGAAGGATGTGGGACAGATTGCAAGGCAAGCAGATAATTTGCATTGGATTTTGGATATTTTGATTGACAGACAAATGGCTGAGGATTTCTTGAAAACATGGGCATCTCAAACTGAGTTGTCTGAATCGCATTCTAAAGTGGCTGCAGTTCATAGATATGAGGTTAGCAGAGTTACAGCTCGACTCTTTGTTGGGATTGGAAAGGGTCAGCTGTTGGCTTCCAAGGACGTGAGGTTCTTGCTTTTGCGTACATGGTTGGTGCCATTTTATGATGATTTTGGATGGATGAGGAGGGCATCAAAAGGCCTTGATAGGCACTTAATTGAGGATGGTCTGAGCAACACAATTCTTACCCTGCCTTTAGCATGGCAACAAGAAATTTTGCTTGCTTGGTTTAATCGGTTCTTGAACTCTGGTGAAGATTGCCCAAATATACAAAGAGGATTTGAAGTTTGGTGGAGGCGGTCCTTTTGGAGGCGGAATGGGGAGCAGGAAAGACCACGGCCATTACGAATTACAGCAGCAACCATTGAGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB

Protein Analysis

625

Amino Acids

70.1

Weight (kDa)

7.26

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB-POZ_ANK-like PF25553 378 - 603 4.8e-64 BTB/POZ Ankyrin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63850
fragaria_vesca FvH4_5g02930 FvH4_5g02930
malus_domestica MD06G1088400.v1.1 MD14G1109500.v1.1
prunus_persica Prupe.5G104400_v2.0.a1
pyrus_communis pycom06g08440 pycom14g10360
rosa_chinensis RchiOBHm_Chr7g0196961
rosa_laevigata RLG00000003977
rosa_multiflora Rmu_sc0001083.1_g000006
rosa_roxburghii Rroxscaffold_3G00258800
rosa_rugosa Rorug07G0037000
rosa_samantha Rh7AG161400 Rh7BG163600 Rh7CG169600 Rh7DG162900
rosa_wichuraiana Rw7G014120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 764
AccB1I GGYRCC 2 cut(s) 994, 1591
AccII CGCG 5 cut(s) 201, 430, 518, 725, 1194
AccIII TCCGGA 1 cut(s) 1251
AclWI GGATC 4 cut(s) 274, 287, 493, 885
AcoI YGGCCR 3 cut(s) 459, 477, 1835
AcsI RAATTY 3 cut(s) 253, 1231, 1710
AcyI GRCGYC 4 cut(s) 79, 513, 785, 995
AfaI GTAC 5 cut(s) 25, 806, 983, 1268, 1582
AfiI CCNNNNNNNGG 4 cut(s) 235, 313, 681, 1806
AhlI ACTAGT 1 cut(s) 20
AjiI CACGTC 2 cut(s) 835, 1561
AjnI CCWGG 3 cut(s) 155, 536, 561
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AloI GAACNNNNNNTCC 2 cut(s) 1550, 1582
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 145, 697, 1242
AlwI GGATC 4 cut(s) 274, 287, 493, 885
AlwNI CAGNNNCTG 1 cut(s) 577
Ama87I CYCGRG 4 cut(s) 146, 361, 620, 1314
Aor13HI TCCGGA 1 cut(s) 1251
AoxI GGCC 4 cut(s) 459, 477, 1636, 1835
ApeKI GCWGC 6 cut(s) 586, 772, 1045, 1319, 1475, 1853
ApoI RAATTY 3 cut(s) 253, 1231, 1710
ArsI GACNNNNNNTTYG 2 cut(s) 775, 807
Asp700I GAANNNNTTC 1 cut(s) 629
AspLEI GCGC 5 cut(s) 520, 577, 674, 727, 997
AspS9I GGNCC 4 cut(s) 432, 559, 598, 1797
AsuC2I CCSGG 6 cut(s) 115, 230, 294, 613, 621, 622
AsuHPI GGTGA 4 cut(s) 38, 252, 1222, 1760
AsuII TTCGAA 1 cut(s) 450
AvaI CYCGRG 4 cut(s) 146, 361, 620, 1314
AvaII GGWCC 4 cut(s) 432, 559, 598, 1797
BalI TGGCCA 2 cut(s) 461, 479
BamHI GGATCC 1 cut(s) 279
BanI GGYRCC 2 cut(s) 994, 1591
BanII GRGCYC 3 cut(s) 225, 471, 620
BbsI GAAGAC 1 cut(s) 783
Bbv12I GWGCWC 1 cut(s) 225
BbvCI CCTCAGC 2 cut(s) 1005, 1413
BbvI GCAGC 6 cut(s) 598, 784, 1032, 1331, 1462, 1865
BccI CCATC 6 cut(s) 60, 253, 451, 1274, 1608, 1655
BceAI ACGGC 6 cut(s) 526, 728, 772, 787, 982, 1850
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 3 cut(s) 157, 538, 563
BcnI CCSGG 6 cut(s) 115, 230, 294, 613, 621, 622
BcoDI GTCTC 3 cut(s) 145, 697, 1242
BcuI ACTAGT 1 cut(s) 20
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 2 cut(s) 30, 1476
BfoI RGCGCY 3 cut(s) 578, 675, 998
BmcAI AGTACT 2 cut(s) 25, 983
Bme1390I CCNGG 9 cut(s) 115, 157, 230, 294, 538, 563, 613, 621, 622
Bme18I GGWCC 4 cut(s) 432, 559, 598, 1797
BmeT110I CYCGRG 4 cut(s) 146, 361, 620, 1314
BmgBI CACGTC 2 cut(s) 835, 1561
BmgT120I GGNCC 4 cut(s) 432, 559, 598, 1797
BmiI GGNNCC 6 cut(s) 281, 610, 617, 626, 996, 1593
BmrFI CCNGG 9 cut(s) 115, 157, 230, 294, 538, 563, 613, 621, 622
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 6 cut(s) 573, 946, 1037, 1209, 1445, 1637
BmuI ACTGGG 1 cut(s) 302
BoxI GACNNNNGTC 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 783
BplI GAGNNNNNCTC 2 cut(s) 355, 387
Bpu10I CCTNAGC 2 cut(s) 1005, 1413
Bpu14I TTCGAA 1 cut(s) 450
BpuMI CCSGG 6 cut(s) 115, 230, 294, 613, 621, 622
BsaBI GATNNNNATC 1 cut(s) 719
BsaHI GRCGYC 4 cut(s) 79, 513, 785, 995
BsaWI WCCGGW 2 cut(s) 282, 1251
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 313, 681, 1806
Bse118I RCCGGY 2 cut(s) 215, 732
Bse1I ACTGG 2 cut(s) 308, 628
Bse8I GATNNNNATC 1 cut(s) 719
BseAI TCCGGA 1 cut(s) 1251
BseBI CCWGG 3 cut(s) 157, 538, 563
BseGI GGATG 4 cut(s) 1345, 1619, 1623, 1666
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 4 cut(s) 235, 313, 681, 1806
BseMII CTCAG 6 cut(s) 872, 996, 1104, 1404, 1437, 1658
BseNI ACTGG 2 cut(s) 308, 628
BseRI GAGGAG 3 cut(s) 1031, 1130, 1636
BseXI GCAGC 6 cut(s) 598, 784, 1032, 1331, 1462, 1865
BseYI CCCAGC 1 cut(s) 1067
BsgI GTGCAG 1 cut(s) 689
Bsh1236I CGCG 5 cut(s) 201, 430, 518, 725, 1194
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 461, 479, 1638, 1837
BshNI GGYRCC 2 cut(s) 994, 1591
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 4 cut(s) 146, 361, 620, 1314
BslFI GGGAC 2 cut(s) 1123, 1359
BslI CCNNNNNNNGG 4 cut(s) 235, 313, 681, 1806
BsmAI GTCTC 3 cut(s) 145, 697, 1242
BsmBI CGTCTC 2 cut(s) 697, 1242
BsmFI GGGAC 2 cut(s) 1123, 1359
BsmI GAATGC 1 cut(s) 1462
BsnI GGCC 4 cut(s) 461, 479, 1638, 1837
BsoBI CYCGRG 4 cut(s) 146, 361, 620, 1314
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 3 cut(s) 225, 471, 620
Bsp13I TCCGGA 1 cut(s) 1251
Bsp143I GATC 3 cut(s) 279, 498, 877
BspANI GGCC 4 cut(s) 461, 479, 1638, 1837
BspCNI CTCAG 6 cut(s) 873, 997, 1105, 1405, 1438, 1659
BspEI TCCGGA 1 cut(s) 1251
BspFNI CGCG 5 cut(s) 201, 430, 518, 725, 1194
BspLI GGNNCC 6 cut(s) 281, 610, 617, 626, 996, 1593
BspMAI CTGCAG 1 cut(s) 1480
BspPI GGATC 4 cut(s) 274, 287, 493, 885
BspT104I TTCGAA 1 cut(s) 450
BspT107I GGYRCC 2 cut(s) 994, 1591
BsrFI RCCGGY 2 cut(s) 215, 732
BsrI ACTGG 2 cut(s) 308, 628
BssAI RCCGGY 2 cut(s) 215, 732
BssMI GATC 3 cut(s) 279, 498, 877
BssNI GRCGYC 4 cut(s) 79, 513, 785, 995
BssT1I CCWWGG 2 cut(s) 675, 1554
Bst2UI CCWGG 3 cut(s) 157, 538, 563
Bst4CI ACNGT 5 cut(s) 264, 341, 650, 710, 827
Bst6I CTCTTC 3 cut(s) 881, 1008, 1076
BstACI GRCGYC 4 cut(s) 79, 513, 785, 995
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 6 cut(s) 221, 463, 467, 573, 1362, 1719
BstDEI CTNAG 7 cut(s) 881, 1005, 1113, 1215, 1413, 1446, 1667
BstDSI CCRYGG 4 cut(s) 199, 428, 998, 1832
BstENI CCTNNNNNAGG 1 cut(s) 1804
BstF5I GGATG 4 cut(s) 1345, 1619, 1623, 1666
BstFNI CGCG 5 cut(s) 201, 430, 518, 725, 1194
BstH2I RGCGCY 3 cut(s) 578, 675, 998
BstHHI GCGC 5 cut(s) 520, 577, 674, 727, 997
BstKTI GATC 3 cut(s) 282, 501, 880
BstMAI GTCTC 3 cut(s) 145, 697, 1242
BstMBI GATC 3 cut(s) 279, 498, 877
BstMCI CGRYCG 1 cut(s) 216
BstNI CCWGG 3 cut(s) 157, 538, 563
BstNSI RCATGY 1 cut(s) 1160
BstPAI GACNNNNGTC 1 cut(s) 137
BstSCI CCNGG 9 cut(s) 113, 155, 228, 292, 536, 561, 611, 619, 620
BstSFI CTRYAG 2 cut(s) 30, 1476
BstUI CGCG 5 cut(s) 201, 430, 518, 725, 1194
BstV1I GCAGC 6 cut(s) 598, 784, 1032, 1331, 1462, 1865
BstV2I GAAGAC 1 cut(s) 783
BstX2I RGATCY 3 cut(s) 279, 498, 877
BstYI RGATCY 3 cut(s) 279, 498, 877
BsuRI GGCC 4 cut(s) 461, 479, 1638, 1837
BtgI CCRYGG 4 cut(s) 199, 428, 998, 1832
BtgZI GCGATG 2 cut(s) 706, 1014
BtrI CACGTC 2 cut(s) 835, 1561
BtsCI GGATG 4 cut(s) 1345, 1619, 1623, 1666
BtsIMutI CAGTG 1 cut(s) 655
Cac8I GCNNGC 6 cut(s) 221, 463, 467, 573, 1362, 1719
CaiI CAGNNNCTG 1 cut(s) 577
CfoI GCGC 5 cut(s) 520, 577, 674, 727, 997
Cfr10I RCCGGY 2 cut(s) 215, 732
Cfr13I GGNCC 4 cut(s) 432, 559, 598, 1797
Cfr42I CCGCGG 2 cut(s) 202, 431
Cfr9I CCCGGG 1 cut(s) 620
CseI GACGC 5 cut(s) 87, 502, 679, 793, 1200
Csp6I GTAC 5 cut(s) 24, 805, 982, 1267, 1581
CviAII CATG 6 cut(s) 313, 550, 1157, 1431, 1584, 1698
CviQI GTAC 5 cut(s) 24, 805, 982, 1267, 1581
DdeI CTNAG 7 cut(s) 881, 1005, 1113, 1215, 1413, 1446, 1667
DinI GGCGCC 1 cut(s) 996
DpnI GATC 3 cut(s) 281, 500, 879
DpnII GATC 3 cut(s) 279, 498, 877
DrdI GACNNNNNNGTC 1 cut(s) 764
DseDI GACNNNNNNGTC 1 cut(s) 764
EaeI YGGCCR 3 cut(s) 459, 477, 1835
Eam1104I CTCTTC 3 cut(s) 881, 1008, 1076
EarI CTCTTC 3 cut(s) 881, 1008, 1076
EciI GGCGGA 1 cut(s) 1825
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 675, 1554
Eco147I AGGCCT 1 cut(s) 1638
Eco24I GRGCYC 3 cut(s) 225, 471, 620
Eco47I GGWCC 4 cut(s) 432, 559, 598, 1797
Eco53kI GAGCTC 1 cut(s) 223
Eco88I CYCGRG 4 cut(s) 146, 361, 620, 1314
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 1804
EcoRI GAATTC 2 cut(s) 253, 1231
EcoRII CCWGG 3 cut(s) 155, 536, 561
EcoT14I CCWWGG 2 cut(s) 675, 1554
EcoT38I GRGCYC 3 cut(s) 225, 471, 620
EgeI GGCGCC 1 cut(s) 996
EheI GGCGCC 1 cut(s) 996
ErhI CCWWGG 2 cut(s) 675, 1554
Esp3I CGTCTC 2 cut(s) 697, 1242
FaeI CATG 6 cut(s) 316, 553, 1160, 1434, 1587, 1701
FaqI GGGAC 2 cut(s) 1123, 1359
FatI CATG 6 cut(s) 312, 549, 1156, 1430, 1583, 1697
FauI CCCGC 5 cut(s) 135, 203, 388, 499, 582
FokI GGATG 4 cut(s) 1352, 1626, 1630, 1673
FriOI GRGCYC 3 cut(s) 225, 471, 620
FspBI CTAG 1 cut(s) 21
GlaI GCGC 5 cut(s) 519, 576, 673, 726, 996
GsaI CCCAGC 1 cut(s) 1071
HaeII RGCGCY 3 cut(s) 578, 675, 998
HaeIII GGCC 4 cut(s) 461, 479, 1638, 1837
HgaI GACGC 5 cut(s) 87, 502, 679, 793, 1200
HhaI GCGC 5 cut(s) 520, 577, 674, 727, 997
Hin1I GRCGYC 4 cut(s) 79, 513, 785, 995
Hin1II CATG 6 cut(s) 316, 553, 1160, 1434, 1587, 1701
Hin6I GCGC 5 cut(s) 518, 575, 672, 725, 995
HinP1I GCGC 5 cut(s) 518, 575, 672, 725, 995
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 1148
HinfI GANTC 6 cut(s) 350, 700, 715, 1259, 1457, 1515
HphI GGTGA 4 cut(s) 38, 252, 1222, 1760
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 8 cut(s) 252, 598, 714, 757, 882, 1042, 1456, 1668
Hpy188III TCNNGA 8 cut(s) 184, 277, 694, 1166, 1226, 1252, 1423, 1738
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 6 cut(s) 136, 268, 271, 518, 787, 799
HpyAV CCTTC 7 cut(s) 892, 925, 1015, 1049, 1163, 1177, 1330
HpyCH4III ACNGT 5 cut(s) 264, 341, 650, 710, 827
HpyCH4IV ACGT 4 cut(s) 665, 834, 906, 1560
HpyCH4V TGCA 7 cut(s) 586, 706, 1156, 1291, 1355, 1375, 1478
HpyF3I CTNAG 7 cut(s) 881, 1005, 1113, 1215, 1413, 1446, 1667
HpySE526I ACGT 4 cut(s) 665, 834, 906, 1560
Hsp92I GRCGYC 4 cut(s) 79, 513, 785, 995
Hsp92II CATG 6 cut(s) 316, 553, 1160, 1434, 1587, 1701
HspAI GCGC 5 cut(s) 518, 575, 672, 725, 995
KasI GGCGCC 1 cut(s) 994
Kpn2I TCCGGA 1 cut(s) 1251
KspI CCGCGG 2 cut(s) 202, 431
Kzo9I GATC 3 cut(s) 279, 498, 877
LmnI GCTCC 5 cut(s) 297, 615, 1042, 1064, 1819
Lsp1109I GCAGC 6 cut(s) 598, 784, 1032, 1331, 1462, 1865
LweI GCATC 6 cut(s) 573, 946, 1037, 1209, 1445, 1637
MaeI CTAG 1 cut(s) 21
MaeII ACGT 4 cut(s) 665, 834, 906, 1560
MaeIII GTNAC 4 cut(s) 341, 650, 1210, 1504
MalI GATC 3 cut(s) 281, 500, 879
MboI GATC 3 cut(s) 279, 498, 877
MboII GAAGA 8 cut(s) 249, 783, 898, 1022, 1025, 1093, 1145, 1763
MfeI CAATTG 2 cut(s) 945, 1292
MflI RGATCY 3 cut(s) 279, 498, 877
MhlI GDGCHC 3 cut(s) 225, 471, 620
MlsI TGGCCA 2 cut(s) 461, 479
MluCI AATT 9 cut(s) 253, 945, 1231, 1292, 1369, 1653, 1677, 1710, 1845
MluNI TGGCCA 2 cut(s) 461, 479
Mly113I GGCGCC 1 cut(s) 995
MlyI GAGTC 3 cut(s) 709, 1268, 1509
MmeI TCCRAC 3 cut(s) 1020, 1042, 1074
Mox20I TGGCCA 2 cut(s) 461, 479
MroI TCCGGA 1 cut(s) 1251
MroXI GAANNNNTTC 1 cut(s) 629
MscI TGGCCA 2 cut(s) 461, 479
MseI TTAA 2 cut(s) 1652, 1728
MslI CAYNNNNRTG 1 cut(s) 1488
Msp20I TGGCCA 2 cut(s) 461, 479
MspA1I CMGCKG 4 cut(s) 201, 430, 589, 1543
MspR9I CCNGG 9 cut(s) 115, 157, 230, 294, 538, 563, 613, 621, 622
MunI CAATTG 2 cut(s) 945, 1292
Mva1269I GAATGC 1 cut(s) 1462
MvaI CCWGG 3 cut(s) 157, 538, 563
MvnI CGCG 5 cut(s) 201, 430, 518, 725, 1194
NarI GGCGCC 1 cut(s) 995
NciI CCSGG 6 cut(s) 115, 230, 294, 613, 621, 622
NdeII GATC 3 cut(s) 279, 498, 877
NlaIII CATG 6 cut(s) 316, 553, 1160, 1434, 1587, 1701
NlaIV GGNNCC 6 cut(s) 281, 610, 617, 626, 996, 1593
NmeAIII GCCGAG 1 cut(s) 1250
NmuCI GTSAC 3 cut(s) 341, 650, 1210
NspI RCATGY 1 cut(s) 1160
NspV TTCGAA 1 cut(s) 450
PaeR7I CTCGAG 1 cut(s) 361
PceI AGGCCT 1 cut(s) 1638
PcsI WCGNNNNNNNCGW 6 cut(s) 345, 359, 687, 791, 800, 1840
PctI GAATGC 1 cut(s) 1462
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 3 cut(s) 350, 715, 1457
PflFI GACNNNGTC 1 cut(s) 787
PfoI TCCNGGA 2 cut(s) 561, 611
PleI GAGTC 3 cut(s) 708, 1267, 1509
PluTI GGCGCC 1 cut(s) 998
PpsI GAGTC 3 cut(s) 708, 1267, 1509
PshAI GACNNNNGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 155, 536, 561
PspFI CCCAGC 1 cut(s) 1067
PspGI CCWGG 3 cut(s) 155, 536, 561
PspN4I GGNNCC 6 cut(s) 281, 610, 617, 626, 996, 1593
PspPI GGNCC 4 cut(s) 432, 559, 598, 1797
PspXI VCTCGAGB 1 cut(s) 361
PstI CTGCAG 1 cut(s) 1480
PstNI CAGNNNCTG 1 cut(s) 577
PsuI RGATCY 3 cut(s) 279, 498, 877
PsyI GACNNNGTC 1 cut(s) 787
PvuII CAGCTG 1 cut(s) 1543
RsaI GTAC 5 cut(s) 25, 806, 983, 1268, 1582
RsaNI GTAC 5 cut(s) 24, 805, 982, 1267, 1581
RseI CAYNNNNRTG 1 cut(s) 1488
SacI GAGCTC 1 cut(s) 225
SacII CCGCGG 2 cut(s) 202, 431
SaqAI TTAA 2 cut(s) 1652, 1728
Sau3AI GATC 3 cut(s) 279, 498, 877
Sau96I GGNCC 4 cut(s) 432, 559, 598, 1797
ScaI AGTACT 2 cut(s) 25, 983
SchI GAGTC 3 cut(s) 709, 1268, 1509
ScrFI CCNGG 9 cut(s) 115, 157, 230, 294, 538, 563, 613, 621, 622
SduI GDGCHC 3 cut(s) 225, 471, 620
SfaNI GCATC 6 cut(s) 573, 946, 1037, 1209, 1445, 1637
SfcI CTRYAG 2 cut(s) 30, 1476
SfoI GGCGCC 1 cut(s) 996
Sfr274I CTCGAG 1 cut(s) 361
Sfr303I CCGCGG 2 cut(s) 202, 431
SfuI TTCGAA 1 cut(s) 450
SgrBI CCGCGG 2 cut(s) 202, 431
SinI GGWCC 4 cut(s) 432, 559, 598, 1797
SlaI CTCGAG 1 cut(s) 361
SmaI CCCGGG 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 1488
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
SpeI ACTAGT 1 cut(s) 20
Sse9I AATT 9 cut(s) 253, 945, 1231, 1292, 1369, 1653, 1677, 1710, 1845
SseBI AGGCCT 1 cut(s) 1638
SspDI GGCGCC 1 cut(s) 994
SspMI CTAG 1 cut(s) 21
SstI GAGCTC 1 cut(s) 225
StuI AGGCCT 1 cut(s) 1638
StyD4I CCNGG 9 cut(s) 113, 155, 228, 292, 536, 561, 611, 619, 620
StyI CCWWGG 2 cut(s) 675, 1554
TaaI ACNGT 5 cut(s) 264, 341, 650, 710, 827
TaiI ACGT 4 cut(s) 668, 837, 909, 1563
TaqI TCGA 6 cut(s) 123, 225, 362, 450, 695, 1513
TasI AATT 9 cut(s) 253, 945, 1231, 1292, 1369, 1653, 1677, 1710, 1845
TatI WGTACW 3 cut(s) 23, 981, 1266
TfiI GAWTC 3 cut(s) 350, 715, 1457
Tru1I TTAA 2 cut(s) 1652, 1728
Tru9I TTAA 2 cut(s) 1652, 1728
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 3 cut(s) 341, 650, 1210
TseI GCWGC 6 cut(s) 586, 772, 1045, 1319, 1475, 1853
Tsp45I GTSAC 3 cut(s) 341, 650, 1210
TspDTI ATGAA 5 cut(s) 911, 1146, 1214, 1260, 1472
TspMI CCCGGG 1 cut(s) 620
TspRI CASTG 1 cut(s) 655
Tth111I GACNNNGTC 1 cut(s) 787
VpaK11BI GGWCC 4 cut(s) 432, 559, 598, 1797
XagI CCTNNNNNAGG 1 cut(s) 1804
XapI RAATTY 3 cut(s) 253, 1231, 1710
XceI RCATGY 1 cut(s) 1160
XhoI CTCGAG 1 cut(s) 361
XmaI CCCGGG 1 cut(s) 620
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 1 cut(s) 21
ZrmI AGTACT 2 cut(s) 25, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.