RLG00000004007

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006809
Physical Location & Seq
Reverse (-)
55197708 .. 55212578
14871 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004007

Sequence Viewer

Length: 7236 bp
ATGACCGGAACGACGCTTACACCGCAGGCGGCAAGTAACTTAGTCAGTCTCAATATTGGTGGCGGCGCCGGGCAGCAGGTCTCGGCTTCGTTCGTCAACTCCTTCCGAGTCACCGCCGTCGCCGACCGGCTGCTCGCTCACGTCCAGTCTGGTTATCGCGGCGATGCCATGGAGTTCTTCAACCTCTGCCTCTCTCTTTCCAGAGGAATTGACTATGCCGTTGCGAACAATGAGGTTCCAGCGAAGGCGTCTGATTTGCCTGTTCTGTTGAGACAGATATGCCAAAGGAAAAACAACGACGTTTTGCTAGCTGCTATCATGGTGCTGATGATATCTGTGAAGAATGCTTGTAGTACTGGATGGTTTTCGGAGAAGGAGAGTGAAGAGCTGTTTTCTCTAGCTAATGAGATCGCCAGTAGGTTCTGTGGCTCGGGGGATATTACAACCGGACCTAGCTGTTCTCGTTCTAATGTTGACAAAGTTATGGAGAGATATTATCCAATGATGAAGATGGGCCACATTCTTGCCTCTCTCGAAGTTAAGCCTGGATATGGAACGTACGTGCTTGATTTCCATATTTCAAAGAGTACCGCGTATTCTCCTCAAGAAAAAATTCGGTTGTTTGTTGCACAAACAGATAATATGGAGACATCTTCTTGTATTATAAGTCCCCAACAAGTGAACTTTCTTCTCAATGGAAAGGGAGTAGATAAAAGGATCACTTGTTCAATGGATACTGGATCACAGTTGCCTTCAAATGTGACCGGAATGCTTAAATATGGAACAAATCTTCTTCAAGCTGTGGGCCAGTTCAATGGTCACTATATCATAGTGGTTGCCTTTATGAGCATCGCATCATCACTTGACACCCCAGTACTGAAAGATTATGTTCAGCCCATCGTACCTTCCTCATCAGATACTGATATTGTTGAGGGGCGATCAAAAATATCACTTAATTGTCCCATAAGCTCTAGGCGTATCAAAACTCCTGTTAAAGGATATTTGTGCAAACATCTTCAGTGCTTCGACTTCGATAACTTTGTGGTTATGAATATAAGACGACCAAGGTGGCGCTGCCCCCATTGTAATCAGTATGTCTGCTATTTAGACATTCGGATCGATCAAAATATGGTTAAGGTCCTGAGAGAGGTAGGAGAGAAATTTTCTCAAGTAATTATCTCTGAAGATGGATCATGGAAGGTTGAGTGGGAAAAAAATGATGATCTAGATGTGGCAGACAAACTGAAAGAAACATCTGAGAAGGATGAAAGTACTTCAGGTCTTAGTTTTATGGACCTTACCAATGATGATGATATTGAGATGGAAACGGCTAGTGCTTGTGGACCTGAAGATGTGAAACCTTTGAGCAATACAAACACTGCTCAACCAGAGGATGACTTTTGGTCTGGAGTTTACTTGGCTAATAGCGGGCTCACCCCTGGCACACAATTGGCTAATTTTATCTCTCACTCTGGTCCCAGTTCTTTACCACCTTCCTTCTTGTCTGATGCTGTTTCTCTTGCACTTAATCGAGAAGCTGGGAGTCATAGAAACACAGATTTTTTGGTGTCTGCAATGCATAATCAACTTTCTACTCCCAATAATGTGAACTCACAACAGTTGCAATCTGCAAATCCGATTGCTAGCAATGAGTATGGGATATCACAAATATTACCCAGAGAACTGGGCTCCGGGCTGCCAATAGTCAGGAATCCAATAGCAGTTCAGGCCCTTCCAGCATCGTCCCCAACAATGGGCTCGCAACATAGACCCAGGACTAGTTTCAATAACTCAGCTCCCAATATTGCCTCCCTCACTTCTCAGGCCGGCCAGTCTATCACTTCCCCAGCAAATGTTCTCAATGCAGTATGTAGCGACTTGGAAAGGCAACAGCACTTCTCTTTGCCCCGAATGAATCCAGTTCAAGTGTCAGACATTGCTTCATCTTCATTGCATCATGGCTCAAGAACAACACAGAATCGGGTTCCCCTAGATCGGTCCTTTACTCATGGTCAATCTACTGTTGGCATCCCGGTTCCAAGCCAATTGCAAAGTGCTAGCAGAGTATCTTCTGGGCTCCAGGGGTTCACAAATGCACACCTACAGCAAACTTTTAATCCGAGGACTCACCCAACTGTGGGCCAGTCTTCAAGTGCACATTCCTCGCGGTCCCATGTGATGCAGGGGAGTGCACAAGTTGGAATTGGTCAAACACCAAGTTCTTTAAACAACCATCAGAGTTTCATAATGGCTGCTCAGCGAGCTGCTCAGGCCAGGCAGTCTTCACCAATGACAGCACAAAATCAAACTCCCAGAACCAGGCCATCTTTGTCTGTAAATGCTGCTGGGTTTAGAGGATCGGCAGGGGATCAGCGTGGAAACATTGGAGGAACTGTCCAAGCAGTTTCAGGGGCTGATGGGTTGGGGAATTCTCCATCTGAACAGAATTGGCGACCAACAGGTCGAATGCGTGGAAGTCTCTCAGGTCAGGCTTATAGTGCTGCTTTGAACCAGTTCATAATTCGGCCAACCCAACCAACTGAAACTGCGAGACCTGCGCCACACCTGACTCCTCCCTCACACCTGACCACTCCATCACACCTGACCCCTCTGCCACACCTGACCCCTCCCCCACACCTGACCCGTCCCCCACACCTGACCCGTTCCTCACACCTGACCTCTCCACCATCTGTTGTTCCATCCCAGCTGCCTACTAATATGAGCGCTCAGTCCGTCGCAACCACTTCGAATGGTGAGTCGGATGTCATGAAGTGCCGATCCGTCGCGTGCATATGGTCCGCCACGCCTCCCTCTCAACGAGTCACCGCCGCCGCCGTCCTCCACCACCCCCCGACTCTGTACACCGGCGGATCCGATGGCTCCGTCATCTGGTGGAACCTCCACTCCTCTCATTCCACCTCGGAACTTGTGCCAATTGCTATGTTGTGTGGCCATGCTGCGCCCATAGCCGACCTGGCCATCTGCGACCCCCTTGCGGTTTCGGAGAGTGAGAAGAGGGACTCTTTGAGCAATGCTGAGCTGAACTCCAGCTGCGGCGCGTTGATAAGTGCTTGTGTTGATGGCATGCTGTGTGTGTGGAGTAGAAGCAGCGGGCATTGCCGGCGGAGGAGGAAGTTGCCGCCTTGGGTGGGCAGTCCTTCCATGGTTAGGACGTTGCCTTCGAATCCGCGGTATGTGTGTGTTGCGTGCTGTTTTGTTGATACTGTCCATTTGAATGACCATCATTCGGTTGAGTCGAGTGAGGTGTTAGTTGATAGGGAGGCTCAGAATAAGAAGCCTTCCAAATGCACTGTGGTTGTTGTTGATTCGTATACTCTTAGCATTGTACAAACTATTTTTCATGGAAACTTGTCCATCGGGTCATTGAAGTTTATGGATGTAGTTTCGTTGACTGAGGGTCAGGAGAAACATTCTGTAGTTATGGCTGATTCATTTGGTCGGTTACAGTTGGTTTCACTACCAAAGGAGCCGCACCAAGATAGGGAGGGAGGGACTGGTTCGCATACAGGTTCTCAGATGGAAAACACGGTTTGTGCTGAGGGATTAAGTGAGGGAGGACAGGTAATGTCAATAGCGACCTGTGAGAATGTTATTGCCTTTGTGTTAAGAAGTCGCTGCATATTTAGGCTATTGCCTACTGGTACTACAGTAGGAGAGATTTCTTTCTTGGACAACAATCTCGTTTGTAAAGAAAGTAACTCCACTCAGTCACATTTTGTGGGAGGGATATTTCTTAAAACTGAAGATGCTGCTGATCTAGAAGCCCAAGAACCCCATGGAATCTTTTCAAGAACTTTTGCTGTTTGGAATAACACAGGTCTTTCAATTGTCTACTTGATATCCTATGTCAGAGATACTTTCAAGTGCGAGCGTCTTTGTGAGATTCCTGCTTCCTCTTATCCCCTAGATGTGAGACTATCAGTCAGCTTCATTCAGTTGAGCCATTATATTCTTCGGATTGAATCAGTTTGCTTAATTGGCGAAGAACATTTGCAGTGGAAACCCCACATCACGATATGGTCTACATGTTGGAAACATGATGACCATGGTAAATTCTGCCTATCATTTAAACTGCATGGAGTAGGCCGTTCTTTTGTTGACTGGACTGCAAATTCTATGCCAAGTAATCAATCTGAGGTTATGCAGACCAAACTGACTTCCTCATACCCTTTTGTTTCAAGCTCCGGAAGTTTAAATAACTTACATGCAGAAATTGACAATCTTGGCTTAGTTAATAAGCGAGGGATAGTGTCTTCTTCCATGGTTATCTCAGAAACTTTCTTTGTTCCATATGCTGTTGTGTATGGCTTCTCTAGTGGAGAAATAGAAATGGTACGGTTTGATTTGTTGGAGGGCCTGGCTTCTCTTGGCGGAAGTCCACGTCATGAGGTGAAGTCATATATGTCCAGACAGCTCTTTTTGGGGCACACAGGTGCTGTGCTCTGCTTGGCAGCACACAGGATGGTAGGCGTTGCCAAAGGATGGAGTTTCAATCAGGTTTTGGTGTCTGGAAGCATGGATTGCACAGTCCGCATATGGGATCTTGACACTGGGAATCTTATCACAGTAATGCACCAGCATGTGGGCCCAGTTCGCCAAATAATTCTTCCCCCAGCTCGTACTTACCGTCCTTGGAGCGACTGTTTTCTCTCAGTTGGGGAGGACTCATGTGTAGCACTTGCTTCTCTCGAGACTTTGCGAGCAGAGAGAATTTTTCCTGGACATCCCAGCTATCCTGCAAAAGTTGTATGGGACAGTGGAAGAGGTTATATTGCATGCTTGTGCCGAAACCATTCAGGAACATCTGATACCGTTGATATCTTGTACATATGGGATGTAAAGACAGGTGCTCGAGAGCGGGTCCTGCGAGGGACGGCATCTCATTCAATGTTTGACCATTTCTGTCAAGGCATCAGCATGAATTCCATTTCTGGCAGTGCATTGAATGGAAATACCTCGGTTTCTTCATTACTTCTTCCAGTAATTGAAGATGGGGCTTCCACGCACTCTCATTTCAATAGTTCGGACAAATTGGCCACTTCATCAAATGTGGTGCCTGGTAATACTGCCAAGTCCAACACTTCCAGAGTCAGTAAGGGCGACTCTGAAAAATTATTTTCAGCCCCTCAAATGGCCATTCAGAGCCGCAAGCATCCAATCACATGCTGTTGCCCTTTCCCTGGAATTGCTGCTCTCAGTTTTGACCTTGCATCATTAGTGTTTCCTTATCAGAAGGATGACCTTATACCAAATAGTCGTGATAAGAAAGAGGATAATGGTGGCAAGGGACAGGGATTTGAGACACCAAGTCCCCGGCACAAGCCTGTAGATAATGGTTCTGGTGTGCTGTCGACCTCAAATGATACTGTTCAAGAGATTGAATGGATTAGAACACTTGAAGAATGTTTACTTCGATTTAGCTTGGGATTCTTGCACTTGTGGAATGTAGATTCTGAGCTTGATAACTTGCTTATAACTGATCTAAATTTGAAGAGACCAGATAGTTTTTTTCTCGCTTCTGGTTTTCAAGGTGACAAAGGGTCTTTGACGTTGACATTTCCTAATTTGAATGCTATTCTTGAGCTCTGGAGAATGTCATCGGAGTTTTGTGCAATCAGGTCACTGACAATGGTGTCTCTTGCCCAACGCATGATTAGCTTGTCGCATGCCAGTTCAAATGCCAGCAGTGCTTTAGCAGCATTTTATACTAGGAACTTTGCAGAGAAAATTCCAGATATAAAGCCGCCTTTACTTCAGCTTCTGGTGAGTTTTTGGCAAGACGAAAGTGAACATGTACGAATGGCTGCACGCTCTTTATTCCACTGTGCTGCTTCTCGAGCAATTCCGCTTCCCCTATGCAGTCAAAAAGCTAATGGCCACTTGAATCTGAGCTCTATAAGTGGTCCTGGAGAGACTGAACATGTAAATTCAAACATGGAAGAAGCATCTACCAACTTATTGTCTTTTAAGTCTGAAGAATCGAATATACATGCATGGCTGGAATCATTTGAAATGCAAGACTGGATTTCTTGTGTTGGGGGGACAAGCCAAGATGCGATGACATCTCATATTATTGTTGCAGCAGCATTGGCTATCTGGTACCCTAGTCTTGTAAAGCCATGTCTTGCCATGCTAGTAGTTCATCCATTGATGAAGCTGGTTATGGCCATGAATGAGAAATATAGTTCAACTGCAGCAGAGCTCCTTGCTGAAGGTATGGAGAGTACATGGAAAGAATGCATCAGTTCTGAAATACCTCGTCTGATTGGGGATATATTTTTCCAGATAGAGTGTGTAAGTGGCCCATCTGCCAATTCATCTGCACAAAATTTGGCTGTTTCAATTGGCCTTCGAGACATGTTGGTTGGTGTTCTTCTTCCAAGTTTAGCTATGGCTGATGTACCAGGGTTTTTAGCTGTTATGGAAAGCCAAATCTGGTCTACTGCATCTGATTCACCTGTTCATTTAGTATCTCTAATGACTCTGATGAGGGTTGTGCGTGGTTCTCCAAGACACCTGGCTCAATACCTAGATAAGGTTATTGACTTCATATTACTAACTGTCGACCCTAGCAACTCAGTCATGCGCAAAACTTGCTTTCAAAGTTCAATGTCTGCTTTAAAGGAAGTTGCACGTGCATTCCCTATGGTAGCGCTGAATGATACATGGACCAAGTTGGCTGTTGGTGATGTGATTGGAGAGAAAAACAATGCTAGCATTCGGGTGTATGACATGCAAAGTATTGTAAGGATAAAAATTTTGGATGCAAGCGGACCTCCCGGACTTCCAAATTTGCTTGCGGCCAGTTCAGAAATGAAACTAGTTACTGCAATTTCAGCTTTGAACTTCTCCCCAGATGGAGAGGGGTTGGTTGCTTTTTCGGAGCATGGGTTGATGATCAGATGGTGGTCATTGGGATCTGTGTGGTGGGAGAAACTGAGTCGAAATTTAGTTCCTGTCCAGTGCACAAAATTAATCTTTGTTCCCCCTTGGGAGGGATTCTCACCTAATTCTACGAGGTCAAGCATAATGGCAAGCATTATGGGACATGATGGGCAAGCCAATATCCAGGAGAATGCAAAAGGTTTGAGTCAGGCGGACAATTTGAAACTATTGATTCATAATCTCGAACTATCTTATCGACTAGAATGGGTTGGTGAACGAAAAGTACATCTTACGAGGCATGGCCATGAGTTAGGCACTTTCCAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

2412

Amino Acids

262.58

Weight (kDa)

6.45

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-MIZ PF02891 316 - 364 2.2e-18 MIZ/SP-RING zinc finger
Beta-prop_WDR3_1st PF25173 1476 - 1582 9.1e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 1479 - 1574 8.6e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1504 - 1541 2.3e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 665, 5495
AasI GACNNNNNNGTC 2 cut(s) 2460, 5653
Acc16I TGCGCA 1 cut(s) 6608
Acc36I ACCTGC 2 cut(s) 67, 2563
Acc65I GGTACC 1 cut(s) 6120
AccB1I GGYRCC 3 cut(s) 65, 5074, 6120
AccB7I CCANNNNNTGG 2 cut(s) 4503, 4603
AccBSI CCGCTC 1 cut(s) 4879
AccI GTMKAC 6 cut(s) 3332, 3852, 4043, 5372, 6461, 6585
AccII CGCG 6 cut(s) 159, 593, 2167, 2786, 3059, 3190
AccIII TCCGGA 1 cut(s) 4205
AcuI CTGAAG 8 cut(s) 1001, 1203, 1260, 1368, 3783, 5759, 6015, 6252
AcvI CACGTG 1 cut(s) 6656
AcyI GRCGYC 2 cut(s) 66, 248
AfeI AGCGCT 2 cut(s) 2725, 6675
AflIII ACRYGT 4 cut(s) 4046, 5812, 5941, 6378
AhdI GACNNNNNGTC 5 cut(s) 1402, 3417, 3941, 5328, 5560
AhlI ACTAGT 2 cut(s) 1778, 6841
AjiI CACGTC 2 cut(s) 142, 4403
AjuI GAANNNNNNNTTGG 2 cut(s) 4611, 4643
AleI CACNNNNGTG 1 cut(s) 4452
AloI GAACNNNNNNTCC 4 cut(s) 709, 741, 2888, 2920
Alw21I GWGCWC 8 cut(s) 2158, 2194, 4464, 4873, 5607, 5915, 6225, 6989
Alw44I GTGCAC 3 cut(s) 2154, 2190, 6985
AlwNI CAGNNNCTG 4 cut(s) 920, 2414, 4457, 5782
Ama87I CYCGRG 4 cut(s) 430, 4709, 4872, 5856
Aor13HI TCCGGA 1 cut(s) 4205
Aor51HI AGCGCT 2 cut(s) 2725, 6675
ApaI GGGCCC 1 cut(s) 4610
ApaLI GTGCAC 3 cut(s) 2154, 2190, 6985
ArsI GACNNNNNNTTYG 4 cut(s) 2741, 2773, 3163, 3195
AseI ATTAAT 1 cut(s) 6995
Asp700I GAANNNNTTC 4 cut(s) 612, 2513, 3805, 4813
Asp718I GGTACC 1 cut(s) 6120
AspLEI GCGC 8 cut(s) 68, 1074, 2560, 2726, 2962, 3059, 6609, 6676
AsuC2I CCSGG 5 cut(s) 70, 1693, 2033, 5335, 6801
AsuII TTCGAA 2 cut(s) 2747, 3182
AsuNHI GCTAGC 4 cut(s) 307, 1643, 2057, 6734
AvaI CYCGRG 4 cut(s) 430, 4709, 4872, 5856
BaeGI GKGCMC 5 cut(s) 2158, 2194, 4449, 4610, 6989
BaeI ACNNNNGTAYC 4 cut(s) 3654, 3687, 6112, 6145
BalI TGGCCA 7 cut(s) 2952, 2978, 5057, 5156, 5898, 6188, 7209
BamHI GGATCC 1 cut(s) 2870
BanI GGYRCC 3 cut(s) 65, 5074, 6120
BanII GRGCYC 8 cut(s) 1434, 1691, 1760, 2079, 4610, 5607, 5915, 6225
BarI GAAGNNNNNNTAC 2 cut(s) 5412, 5444
BbrPI CACGTG 1 cut(s) 6656
BbsI GAAGAC 3 cut(s) 2139, 2274, 4266
Bbv12I GWGCWC 8 cut(s) 2158, 2194, 4464, 4873, 5607, 5915, 6225, 6989
BbvCI CCTCAGC 1 cut(s) 3558
BceAI ACGGC 6 cut(s) 101, 203, 1344, 2819, 4092, 4911
BcgI CGANNNNNNTGC 6 cut(s) 2145, 2179, 2727, 2761, 2975, 3009
BciVI GTATCC 1 cut(s) 727
BclI TGATCA 1 cut(s) 6918
BcnI CCSGG 5 cut(s) 70, 1693, 2033, 5335, 6801
BcuI ACTAGT 2 cut(s) 1778, 6841
BfmI CTRYAG 5 cut(s) 2102, 3435, 3666, 5346, 6213
BfoI RGCGCY 4 cut(s) 69, 1075, 2727, 6677
BfuAI ACCTGC 2 cut(s) 67, 2563
BfuI GTATCC 1 cut(s) 727
BglI GCCNNNNNGGC 1 cut(s) 2975
BlpI GCTNAGC 2 cut(s) 2256, 3036
BmcAI AGTACT 3 cut(s) 355, 876, 1273
BmeRI GACNNNNNGTC 5 cut(s) 1402, 3417, 3941, 5328, 5560
BmeT110I CYCGRG 4 cut(s) 430, 4709, 4872, 5856
BmgBI CACGTC 2 cut(s) 142, 4403
BmrI ACTGGG 5 cut(s) 866, 1473, 1694, 4581, 4604
BmtI GCTAGC 4 cut(s) 311, 1647, 2061, 6738
BmuI ACTGGG 5 cut(s) 866, 1473, 1694, 4581, 4604
BpiI GAAGAC 3 cut(s) 2139, 2274, 4266
BplI GAGNNNNNCTC 4 cut(s) 3029, 3061, 7007, 7039
BpmI CTGGAG 5 cut(s) 1428, 2063, 3031, 5629, 5949
Bpu10I CCTNAGC 2 cut(s) 2268, 3558
Bpu1102I GCTNAGC 2 cut(s) 2256, 3036
Bpu14I TTCGAA 2 cut(s) 2747, 3182
BpuEI CTTGAG 4 cut(s) 588, 1152, 1948, 5621
BpuMI CCSGG 5 cut(s) 70, 1693, 2033, 5335, 6801
Bsa29I ATCGAT 1 cut(s) 1119
BsaAI YACGTR 2 cut(s) 562, 6656
BsaHI GRCGYC 2 cut(s) 66, 248
BsaI GGTCTC 3 cut(s) 85, 2545, 5509
BsaWI WCCGGW 4 cut(s) 5, 446, 764, 4205
Bse118I RCCGGY 4 cut(s) 126, 1826, 2864, 3120
Bse3DI GCAATG 6 cut(s) 1581, 1654, 1935, 1949, 3037, 3115
BseAI TCCGGA 1 cut(s) 4205
BseCI ATCGAT 1 cut(s) 1119
BseMI GCAATG 6 cut(s) 1581, 1654, 1935, 1949, 3037, 3115
BseRI GAGGAG 4 cut(s) 591, 2562, 2896, 3142
BseSI GKGCMC 5 cut(s) 2158, 2194, 4449, 4610, 6989
BseYI CCCAGC 6 cut(s) 1538, 1846, 2345, 2703, 4633, 4748
BsgI GTGCAG 2 cut(s) 5811, 6327
Bsh1236I CGCG 6 cut(s) 159, 593, 2167, 2786, 3059, 3190
Bsh1285I CGRYCG 1 cut(s) 127
BshNI GGYRCC 3 cut(s) 65, 5074, 6120
BshVI ATCGAT 1 cut(s) 1119
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 8 cut(s) 2158, 2194, 4464, 4873, 5607, 5915, 6225, 6989
BsiHKCI CYCGRG 4 cut(s) 430, 4709, 4872, 5856
BsiWI CGTACG 1 cut(s) 558
BsmI GAATGC 8 cut(s) 349, 774, 2472, 5596, 6263, 6659, 6738, 7102
Bso31I GGTCTC 3 cut(s) 85, 2545, 5509
BsoBI CYCGRG 4 cut(s) 430, 4709, 4872, 5856
Bsp119I TTCGAA 2 cut(s) 2747, 3182
Bsp120I GGGCCC 1 cut(s) 4606
Bsp13I TCCGGA 1 cut(s) 4205
Bsp1407I TGTACA 3 cut(s) 2859, 3346, 4845
Bsp1720I GCTNAGC 2 cut(s) 2256, 3036
Bsp19I CCATGG 5 cut(s) 168, 3162, 3796, 4066, 4281
BspDI ATCGAT 1 cut(s) 1119
BspEI TCCGGA 1 cut(s) 4205
BspFNI CGCG 6 cut(s) 159, 593, 2167, 2786, 3059, 3190
BspHI TCATGA 2 cut(s) 2766, 4405
BspMAI CTGCAG 1 cut(s) 6217
BspMI ACCTGC 2 cut(s) 67, 2563
BspOI GCTAGC 4 cut(s) 311, 1647, 2061, 6738
BspQI GCTCTTC 1 cut(s) 378
BspT104I TTCGAA 2 cut(s) 2747, 3182
BspT107I GGYRCC 3 cut(s) 65, 5074, 6120
BspTNI GGTCTC 3 cut(s) 85, 2545, 5509
BsrBI CCGCTC 1 cut(s) 4879
BsrDI GCAATG 6 cut(s) 1581, 1654, 1935, 1949, 3037, 3115
BsrFI RCCGGY 4 cut(s) 126, 1826, 2864, 3120
BsrGI TGTACA 3 cut(s) 2859, 3346, 4845
BssAI RCCGGY 4 cut(s) 126, 1826, 2864, 3120
BssNAI GTATAC 1 cut(s) 3333
BssNI GRCGYC 2 cut(s) 66, 248
BssT1I CCWWGG 9 cut(s) 168, 1064, 3143, 3162, 3796, 4066, 4281, 4652, 7010
Bst1107I GTATAC 1 cut(s) 3333
Bst6I CTCTTC 4 cut(s) 378, 3008, 4777, 5507
BstACI GRCGYC 2 cut(s) 66, 248
BstAPI GCANNNNNTGC 2 cut(s) 4542, 6615
BstAUI TGTACA 3 cut(s) 2859, 3346, 4845
BstBAI YACGTR 2 cut(s) 562, 6656
BstBI TTCGAA 2 cut(s) 2747, 3182
BstDSI CCRYGG 6 cut(s) 168, 3162, 3188, 3796, 4066, 4281
BstENI CCTNNNNNAGG 3 cut(s) 993, 1145, 6554
BstFNI CGCG 6 cut(s) 159, 593, 2167, 2786, 3059, 3190
BstH2I RGCGCY 4 cut(s) 69, 1075, 2727, 6677
BstHHI GCGC 8 cut(s) 68, 1074, 2560, 2726, 2962, 3059, 6609, 6676
BstMCI CGRYCG 1 cut(s) 127
BstSFI CTRYAG 5 cut(s) 2102, 3435, 3666, 5346, 6213
BstSLI GKGCMC 5 cut(s) 2158, 2194, 4449, 4610, 6989
BstUI CGCG 6 cut(s) 159, 593, 2167, 2786, 3059, 3190
BstV2I GAAGAC 3 cut(s) 2139, 2274, 4266
BstX2I RGATCY 3 cut(s) 2870, 4561, 6938
BstXI CCANNNNNNTGG 2 cut(s) 815, 1684
BstYI RGATCY 3 cut(s) 2870, 4561, 6938
BstZ17I GTATAC 1 cut(s) 3333
Bsu15I ATCGAT 1 cut(s) 1119
BsuI GTATCC 1 cut(s) 727
BsuTUI ATCGAT 1 cut(s) 1119
BtgI CCRYGG 6 cut(s) 168, 3162, 3188, 3796, 4066, 4281
BtgZI GCGATG 3 cut(s) 177, 835, 6092
BtrI CACGTC 2 cut(s) 142, 4403
BtsI GCAGTG 4 cut(s) 1377, 4022, 4963, 5713
BveI ACCTGC 2 cut(s) 67, 2563
CaiI CAGNNNCTG 4 cut(s) 920, 2414, 4457, 5782
CciI TCATGA 2 cut(s) 2766, 4405
CfoI GCGC 8 cut(s) 68, 1074, 2560, 2726, 2962, 3059, 6609, 6676
Cfr10I RCCGGY 4 cut(s) 126, 1826, 2864, 3120
Cfr42I CCGCGG 1 cut(s) 3191
ClaI ATCGAT 1 cut(s) 1119
CseI GACGC 3 cut(s) 22, 237, 3881
CspCI CAANNNNNGTGG 2 cut(s) 40, 75
DinI GGCGCC 1 cut(s) 67
DraI TTTAAA 4 cut(s) 2226, 4090, 4215, 6642
DrdI GACNNNNNNGTC 2 cut(s) 2460, 5653
DriI GACNNNNNGTC 5 cut(s) 1402, 3417, 3941, 5328, 5560
DseDI GACNNNNNNGTC 2 cut(s) 2460, 5653
Eam1104I CTCTTC 4 cut(s) 378, 3008, 4777, 5507
Eam1105I GACNNNNNGTC 5 cut(s) 1402, 3417, 3941, 5328, 5560
EarI CTCTTC 4 cut(s) 378, 3008, 4777, 5507
EciI GGCGGA 5 cut(s) 2788, 2883, 3139, 4407, 7133
Ecl136II GAGCTC 3 cut(s) 5605, 5913, 6223
Eco130I CCWWGG 9 cut(s) 168, 1064, 3143, 3162, 3796, 4066, 4281, 4652, 7010
Eco24I GRGCYC 8 cut(s) 1434, 1691, 1760, 2079, 4610, 5607, 5915, 6225
Eco31I GGTCTC 3 cut(s) 85, 2545, 5509
Eco32I GATATC 4 cut(s) 333, 1662, 3861, 4840
Eco47III AGCGCT 2 cut(s) 2725, 6675
Eco53kI GAGCTC 3 cut(s) 5605, 5913, 6223
Eco57I CTGAAG 8 cut(s) 1001, 1203, 1260, 1368, 3783, 5759, 6015, 6252
Eco72I CACGTG 1 cut(s) 6656
Eco88I CYCGRG 4 cut(s) 430, 4709, 4872, 5856
EcoICRI GAGCTC 3 cut(s) 5605, 5913, 6223
EcoNI CCTNNNNNAGG 3 cut(s) 993, 1145, 6554
EcoO109I RGGNCCY 4 cut(s) 1138, 1729, 4375, 4882
EcoRI GAATTC 2 cut(s) 2428, 4942
EcoRV GATATC 4 cut(s) 333, 1662, 3861, 4840
EcoT14I CCWWGG 9 cut(s) 168, 1064, 3143, 3162, 3796, 4066, 4281, 4652, 7010
EcoT22I ATGCAT 3 cut(s) 1581, 6016, 6263
EcoT38I GRGCYC 8 cut(s) 1434, 1691, 1760, 2079, 4610, 5607, 5915, 6225
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 9 cut(s) 168, 1064, 3143, 3162, 3796, 4066, 4281, 4652, 7010
FalI AAGNNNNNCTT 4 cut(s) 706, 738, 7179, 7211
FauI CCCGC 3 cut(s) 1421, 3104, 4872
FauNDI CATATG 4 cut(s) 2792, 4312, 4556, 4850
FbaI TGATCA 1 cut(s) 6918
FblI GTMKAC 6 cut(s) 3332, 3852, 4043, 5372, 6461, 6585
FriOI GRGCYC 8 cut(s) 1434, 1691, 1760, 2079, 4610, 5607, 5915, 6225
FseI GGCCGGCC 1 cut(s) 1830
FspI TGCGCA 1 cut(s) 6608
GlaI GCGC 8 cut(s) 67, 1073, 2559, 2725, 2961, 3058, 6608, 6675
GsaI CCCAGC 6 cut(s) 1542, 1850, 2349, 2707, 4637, 4752
GsuI CTGGAG 5 cut(s) 1428, 2063, 3031, 5629, 5949
HaeII RGCGCY 4 cut(s) 69, 1075, 2727, 6677
HgaI GACGC 3 cut(s) 22, 237, 3881
HhaI GCGC 8 cut(s) 68, 1074, 2560, 2726, 2962, 3059, 6609, 6676
Hin1I GRCGYC 2 cut(s) 66, 248
Hin6I GCGC 8 cut(s) 66, 1072, 2558, 2724, 2960, 3057, 6607, 6674
HinP1I GCGC 8 cut(s) 66, 1072, 2558, 2724, 2960, 3057, 6607, 6674
HincII GTYRAC 7 cut(s) 97, 475, 3411, 4120, 5373, 5574, 6586
HindII GTYRAC 7 cut(s) 97, 475, 3411, 4120, 5373, 5574, 6586
Hpy99I CGWCG 5 cut(s) 16, 122, 302, 2738, 2786
HpyCH4IV ACGT 8 cut(s) 141, 300, 557, 561, 3173, 4402, 5570, 6655
HpySE526I ACGT 8 cut(s) 141, 300, 557, 561, 3173, 4402, 5570, 6655
Hsp92I GRCGYC 2 cut(s) 66, 248
HspAI GCGC 8 cut(s) 66, 1072, 2558, 2724, 2960, 3057, 6607, 6674
KasI GGCGCC 1 cut(s) 65
Kpn2I TCCGGA 1 cut(s) 4205
KpnI GGTACC 1 cut(s) 6124
KroI GCCGGC 2 cut(s) 1826, 3120
KroNI GCCGGC 2 cut(s) 1828, 3122
Ksp22I TGATCA 1 cut(s) 6918
KspI CCGCGG 1 cut(s) 3191
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 9 cut(s) 1694, 1801, 2082, 2885, 3487, 4208, 4656, 6228, 6904
MaeII ACGT 8 cut(s) 141, 300, 557, 561, 3173, 4402, 5570, 6655
MbiI CCGCTC 1 cut(s) 4879
MfeI CAATTG 5 cut(s) 1448, 2045, 2934, 3846, 6363
MflI RGATCY 3 cut(s) 2870, 4561, 6938
MlsI TGGCCA 7 cut(s) 2952, 2978, 5057, 5156, 5898, 6188, 7209
MluNI TGGCCA 7 cut(s) 2952, 2978, 5057, 5156, 5898, 6188, 7209
Mly113I GGCGCC 1 cut(s) 66
MmeI TCCRAC 5 cut(s) 2179, 2739, 4031, 4350, 5121
Mox20I TGGCCA 7 cut(s) 2952, 2978, 5057, 5156, 5898, 6188, 7209
Mph1103I ATGCAT 3 cut(s) 1581, 6016, 6263
MroI TCCGGA 1 cut(s) 4205
MroNI GCCGGC 2 cut(s) 1826, 3120
MroXI GAANNNNTTC 4 cut(s) 612, 2513, 3805, 4813
MscI TGGCCA 7 cut(s) 2952, 2978, 5057, 5156, 5898, 6188, 7209
MslI CAYNNNNRTG 8 cut(s) 3234, 4452, 4589, 4599, 4801, 4937, 6743, 7209
Msp20I TGGCCA 7 cut(s) 2952, 2978, 5057, 5156, 5898, 6188, 7209
MspA1I CMGCKG 4 cut(s) 2707, 3051, 3111, 3190
MunI CAATTG 5 cut(s) 1448, 2045, 2934, 3846, 6363
Mva1269I GAATGC 8 cut(s) 349, 774, 2472, 5596, 6263, 6659, 6738, 7102
MvnI CGCG 6 cut(s) 159, 593, 2167, 2786, 3059, 3190
NaeI GCCGGC 2 cut(s) 1828, 3122
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 5 cut(s) 70, 1693, 2033, 5335, 6801
NcoI CCATGG 5 cut(s) 168, 3162, 3796, 4066, 4281
NdeI CATATG 4 cut(s) 2792, 4312, 4556, 4850
NgoMIV GCCGGC 2 cut(s) 1826, 3120
NheI GCTAGC 4 cut(s) 307, 1643, 2057, 6734
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 7 cut(s) 109, 760, 818, 2821, 3729, 5552, 5640
NsbI TGCGCA 1 cut(s) 6608
NsiI ATGCAT 3 cut(s) 1581, 6016, 6263
NspV TTCGAA 2 cut(s) 2747, 3182
OliI CACNNNNGTG 1 cut(s) 4452
PaeI GCATGC 3 cut(s) 3088, 4800, 5690
PaeR7I CTCGAG 3 cut(s) 4709, 4872, 5856
PagI TCATGA 2 cut(s) 2766, 4405
PciI ACATGT 4 cut(s) 4046, 5812, 5941, 6378
PciSI GCTCTTC 1 cut(s) 378
PcsI WCGNNNNNNNCGW 3 cut(s) 3179, 3254, 4645
PctI GAATGC 8 cut(s) 349, 774, 2472, 5596, 6263, 6659, 6738, 7102
PdiI GCCGGC 2 cut(s) 1828, 3122
PdmI GAANNNNTTC 4 cut(s) 612, 2513, 3805, 4813
Pfl23II CGTACG 1 cut(s) 558
PflMI CCANNNNNTGG 2 cut(s) 4503, 4603
PfoI TCCNGGA 4 cut(s) 4738, 5926, 6799, 7089
PluTI GGCGCC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 6656
PmlI CACGTG 1 cut(s) 6656
Ppu21I YACGTR 2 cut(s) 562, 6656
PpuMI RGGWCCY 2 cut(s) 1138, 4882
PscI ACATGT 4 cut(s) 4046, 5812, 5941, 6378
PshBI ATTAAT 1 cut(s) 6995
PsiI TTATAA 2 cut(s) 665, 5495
Psp124BI GAGCTC 3 cut(s) 5607, 5915, 6225
Psp5II RGGWCCY 2 cut(s) 1138, 4882
PspCI CACGTG 1 cut(s) 6656
PspFI CCCAGC 6 cut(s) 1538, 1846, 2345, 2703, 4633, 4748
PspLI CGTACG 1 cut(s) 558
PspOMI GGGCCC 1 cut(s) 4606
PspPPI RGGWCCY 2 cut(s) 1138, 4882
PsrI GAACNNNNNNTAC 5 cut(s) 33, 4813, 4845, 7173, 7205
PstI CTGCAG 1 cut(s) 6217
PstNI CAGNNNCTG 4 cut(s) 920, 2414, 4457, 5782
PsuI RGATCY 3 cut(s) 2870, 4561, 6938
PvuII CAGCTG 2 cut(s) 2707, 3051
RigI GGCCGGCC 1 cut(s) 1830
RseI CAYNNNNRTG 8 cut(s) 3234, 4452, 4589, 4599, 4801, 4937, 6743, 7209
SacI GAGCTC 3 cut(s) 5607, 5915, 6225
SacII CCGCGG 1 cut(s) 3191
SalI GTCGAC 2 cut(s) 5371, 6584
SapI GCTCTTC 1 cut(s) 378
ScaI AGTACT 3 cut(s) 355, 876, 1273
SfcI CTRYAG 5 cut(s) 2102, 3435, 3666, 5346, 6213
SfoI GGCGCC 1 cut(s) 67
Sfr274I CTCGAG 3 cut(s) 4709, 4872, 5856
Sfr303I CCGCGG 1 cut(s) 3191
SfuI TTCGAA 2 cut(s) 2747, 3182
SgrAI CRCCGGYG 1 cut(s) 2864
SgrBI CCGCGG 1 cut(s) 3191
SlaI CTCGAG 3 cut(s) 4709, 4872, 5856
SmiMI CAYNNNNRTG 8 cut(s) 3234, 4452, 4589, 4599, 4801, 4937, 6743, 7209
SmlI CTYRAG 7 cut(s) 603, 1167, 1963, 4709, 4872, 5600, 5856
SmoI CTYRAG 7 cut(s) 603, 1167, 1963, 4709, 4872, 5600, 5856
SpeI ACTAGT 2 cut(s) 1778, 6841
SphI GCATGC 3 cut(s) 3088, 4800, 5690
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 3 cut(s) 55, 1671, 1804
SstI GAGCTC 3 cut(s) 5607, 5915, 6225
StyI CCWWGG 9 cut(s) 168, 1064, 3143, 3162, 3796, 4066, 4281, 4652, 7010
TaiI ACGT 8 cut(s) 144, 303, 560, 564, 3176, 4405, 5573, 6658
TaqII GACCGA 1 cut(s) 1986
TatI WGTACW 8 cut(s) 353, 874, 1271, 2859, 3346, 4845, 6245, 7189
TseFI GTSAC 7 cut(s) 109, 760, 818, 2821, 3729, 5552, 5640
Tsp45I GTSAC 7 cut(s) 109, 760, 818, 2821, 3729, 5552, 5640
TspGWI ACGGA 3 cut(s) 2722, 2770, 2872
Van91I CCANNNNNTGG 2 cut(s) 4503, 4603
VneI GTGCAC 3 cut(s) 2154, 2190, 6985
VspI ATTAAT 1 cut(s) 6995
XagI CCTNNNNNAGG 3 cut(s) 993, 1145, 6554
XbaI TCTAGA 2 cut(s) 1225, 3778
XcmI CCANNNNNNNNNTGG 3 cut(s) 2971, 3310, 3794
XhoI CTCGAG 3 cut(s) 4709, 4872, 5856
XmiI GTMKAC 6 cut(s) 3332, 3852, 4043, 5372, 6461, 6585
XmnI GAANNNNTTC 4 cut(s) 612, 2513, 3805, 4813
ZrmI AGTACT 3 cut(s) 355, 876, 1273
Zsp2I ATGCAT 3 cut(s) 1581, 6016, 6263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.