RLG00000006016

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018178
Physical Location & Seq
Forward (+)
3536390 .. 3540064
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006016

Sequence Viewer

Length: 1200 bp
ATGGCGAAGCAACCACCCATAGGGCCAAAGCGTCCGGCCCAGAAGAAGAAGGGCCATTCTTCCGATTGGGTCTCCGCGACAGATCCCTCAGACGGCTCGCGAAGATGGATGGGACAGATTGGAGATGCTCATGGCGGTGTTGCTGCTCCAAACCCAATTCAAGCATCGTTTGGAAATGCTCATGGAGGTGTTGCTGCTACAAACCCAGTTCAAGCATCGGTTGGAAATGTCAACAAAGAAAATGCCATAAAGGTTGAAAAGCTCCGCCCAGACATGTCGGATGTCACTCTCACTGTAAAGGTTGTTAGTGCTAAGATAGTAGGTATGCAATTTGCTGACTGCTTGGTTGGTGATGAAACTGGAATGATAATCTTCAGAGCTAAAAACGATCAAGTGGACTTGATGAAAGTGGGTTTGACTGTAACTCTGCAAAATGCCAGAGTTGACATGTTCAATGATTCAATGAGGCTTGTTGTTGACGAAAGTGGCAGTGTTCAAGTTGCTGAGCCTGCCAGTTTTACCGTGAAGGAAGATAGCAACCTCTCACTCATTCAAGCATCATTTGGAAATGTCAACAAAGATAATGAGATGCGTGATGCCACAAAGGTTGAAAAGCTCCGCCCAGACATGTCGGATGTCACTCTCACTGTAAAGGTTGTTAGTACTAGAATACTAGGTCGGAAGGGACGTCCTGGTGGCCCTCGAAGCCATATGCAATTTGCTGACTGCTTGGTTGGCGATGAAACTGGAATGATAAACTTCAGAGCTACAAACGATCAAGTGGACTTGATGAAAGAGGGTTTGACTGTAACTCTGCAAAATGCCAAAGTTGACATGTTCAGAGATTCAATGAGGGTTGTTGTCGATGAAAGTGGCAGTGTTGAAGTTGCTGAGCCTGCCAGTTTTACTGTGAAGGAAGATAGCAACCTCTCACTTATTCAATTTGACCGTCAAAAAAAGGAGGGGCCACGTGAACTGGAGGTGTACGAGCAGGGTATGCCCAAGACTGAGCTTTGCTTGAATTGGCAGGACTCTGGTTACTGCCGTTGCAAAGACCACTGCAAATTTGCTCACGGCATGGAGGAGCTGCGCCAGGTGAACCGCCACCCGCTCTACAAGACCAAGGTCTGCCAATATTGGCTTGCCGATGAGCCCTGTGGATTCGGCCGTCGCTGCCATTTTCGCCACACACTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

44.06

Weight (kDa)

7.1

Isoelectric Point (pI)

28.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 88 - 167 7.2e-25 Single-stranded DNA binding protein Ssb-like, OB fold
OB_Ssb-like PF21473 206 - 296 2e-29 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018178)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0442351 RchiOBHm_Chr7g0219251
rosa_laevigata RLG00000006016
rosa_multiflora Rmu_co8418355.1_g000001 Rmu_sc0000124.1_g000049
rosa_roxburghii Rroxscaffold_5G00383220
rosa_rugosa Rorug04G0340500
rosa_samantha Rh4AG392200 Rh4BG405700 Rh4CG420800 Rh4DG398900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 691
AccBSI CCGCTC 1 cut(s) 1111
AccII CGCG 2 cut(s) 77, 100
AciI CCGC 6 cut(s) 75, 135, 265, 619, 1102, 1109
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 1165
AcsI RAATTY 1 cut(s) 1064
AcuI CTGAAG 2 cut(s) 358, 745
AcvI CACGTG 1 cut(s) 971
AcyI GRCGYC 1 cut(s) 688
AfaI GTAC 2 cut(s) 664, 986
AfiI CCNNNNNNNGG 2 cut(s) 20, 92
AflIII ACRYGT 4 cut(s) 273, 447, 627, 834
AjnI CCWGG 2 cut(s) 691, 1092
AluBI AGCT 6 cut(s) 262, 380, 616, 767, 1012, 1087
AluI AGCT 6 cut(s) 262, 380, 616, 767, 1012, 1087
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 77
AoxI GGCC 6 cut(s) 23, 36, 52, 697, 965, 1165
ApeKI GCWGC 4 cut(s) 143, 194, 1087, 1173
ApoI RAATTY 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 1092
AspS9I GGNCC 5 cut(s) 23, 37, 52, 698, 965
AsuHPI GGTGA 2 cut(s) 362, 1108
BanII GRGCYC 1 cut(s) 1155
BbrPI CACGTG 1 cut(s) 971
BbvI GCAGC 4 cut(s) 130, 181, 1074, 1160
BccI CCATC 2 cut(s) 99, 103
BceAI ACGGC 4 cut(s) 109, 1029, 1090, 1152
BciT130I CCWGG 2 cut(s) 693, 1094
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 2 cut(s) 666, 674
BisI GCNGC 4 cut(s) 144, 195, 1088, 1174
BlpI GCTNAGC 2 cut(s) 504, 891
BlsI GCNGC 4 cut(s) 145, 196, 1089, 1175
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 2 cut(s) 693, 1094
BmgT120I GGNCC 5 cut(s) 23, 37, 52, 698, 965
BmiI GGNNCC 1 cut(s) 966
BmrFI CCNGG 2 cut(s) 693, 1094
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 6 cut(s) 115, 173, 224, 566, 579, 586
BmuI ACTGGG 1 cut(s) 200
BoxI GACNNNNGTC 1 cut(s) 1124
BpmI CTGGAG 1 cut(s) 998
Bpu1102I GCTNAGC 2 cut(s) 504, 891
BsaAI YACGTR 1 cut(s) 971
BsaHI GRCGYC 1 cut(s) 688
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 1122
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 92
Bse1I ACTGG 6 cut(s) 206, 364, 513, 751, 900, 981
BseBI CCWGG 2 cut(s) 693, 1094
BseDI CCNNGG 1 cut(s) 1122
BseGI GGATG 3 cut(s) 114, 286, 640
BseLI CCNNNNNNNGG 2 cut(s) 20, 92
BseMII CTCAG 4 cut(s) 102, 495, 882, 999
BseNI ACTGG 6 cut(s) 206, 364, 513, 751, 900, 981
BseRI GAGGAG 1 cut(s) 1097
BseX3I CGGCCG 1 cut(s) 1165
BseXI GCAGC 4 cut(s) 130, 181, 1074, 1160
Bsh1236I CGCG 2 cut(s) 77, 100
Bsh1285I CGRYCG 1 cut(s) 1168
BshFI GGCC 6 cut(s) 25, 38, 54, 699, 967, 1167
BsiEI CGRYCG 1 cut(s) 1168
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 2 cut(s) 126, 699
BslI CCNNNNNNNGG 2 cut(s) 20, 92
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 126, 699
BsnI GGCC 6 cut(s) 25, 38, 54, 699, 967, 1167
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 1155
Bsp143I GATC 3 cut(s) 82, 388, 775
Bsp1720I GCTNAGC 2 cut(s) 504, 891
Bsp68I TCGCGA 1 cut(s) 100
BspACI CCGC 6 cut(s) 75, 135, 265, 619, 1102, 1109
BspANI GGCC 6 cut(s) 25, 38, 54, 699, 967, 1167
BspCNI CTCAG 4 cut(s) 101, 496, 883, 1000
BspFNI CGCG 2 cut(s) 77, 100
BspLI GGNNCC 1 cut(s) 966
BspPI GGATC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 1111
BsrI ACTGG 6 cut(s) 206, 364, 513, 751, 900, 981
BssECI CCNNGG 1 cut(s) 1122
BssMI GATC 3 cut(s) 82, 388, 775
BssNI GRCGYC 1 cut(s) 688
BssT1I CCWWGG 1 cut(s) 1122
Bst2UI CCWGG 2 cut(s) 693, 1094
Bst4CI ACNGT 7 cut(s) 295, 421, 523, 649, 808, 910, 950
BstACI GRCGYC 1 cut(s) 688
BstAPI GCANNNNNTGC 1 cut(s) 997
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 4 cut(s) 98, 510, 897, 1143
BstDEI CTNAG 5 cut(s) 88, 312, 504, 891, 1008
BstF5I GGATG 3 cut(s) 114, 286, 640
BstFNI CGCG 2 cut(s) 77, 100
BstHHI GCGC 1 cut(s) 1092
BstKTI GATC 3 cut(s) 85, 391, 778
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 3 cut(s) 82, 388, 775
BstMCI CGRYCG 1 cut(s) 1168
BstMWI GCNNNNNNNGC 7 cut(s) 509, 705, 735, 896, 997, 1173, 1182
BstNI CCWGG 2 cut(s) 693, 1094
BstNSI RCATGY 4 cut(s) 277, 451, 631, 838
BstPAI GACNNNNGTC 1 cut(s) 1124
BstSCI CCNGG 2 cut(s) 691, 1092
BstUI CGCG 2 cut(s) 77, 100
BstV1I GCAGC 4 cut(s) 130, 181, 1074, 1160
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BstZI CGGCCG 1 cut(s) 1165
BsuRI GGCC 6 cut(s) 25, 38, 54, 699, 967, 1167
BtgZI GCGATG 1 cut(s) 753
BtsCI GGATG 3 cut(s) 114, 286, 640
BtsI GCAGTG 3 cut(s) 496, 883, 1057
BtsIMutI CAGTG 5 cut(s) 291, 496, 645, 883, 1057
BtuMI TCGCGA 1 cut(s) 100
Cac8I GCNNGC 4 cut(s) 98, 510, 897, 1143
CfoI GCGC 1 cut(s) 1092
Cfr13I GGNCC 5 cut(s) 23, 37, 52, 698, 965
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 663, 985
CspCI CAANNNNNGTGG 2 cut(s) 589, 624
CviAII CATG 7 cut(s) 131, 182, 274, 448, 628, 835, 1078
CviQI GTAC 2 cut(s) 663, 985
DdeI CTNAG 5 cut(s) 88, 312, 504, 891, 1008
DpnI GATC 3 cut(s) 84, 390, 777
DpnII GATC 3 cut(s) 82, 388, 775
EaeI YGGCCR 1 cut(s) 1165
EagI CGGCCG 1 cut(s) 1165
EciI GGCGGA 2 cut(s) 254, 608
EclXI CGGCCG 1 cut(s) 1165
Eco130I CCWWGG 1 cut(s) 1122
Eco24I GRGCYC 1 cut(s) 1155
Eco31I GGTCTC 1 cut(s) 76
Eco52I CGGCCG 1 cut(s) 1165
Eco57I CTGAAG 2 cut(s) 358, 745
Eco72I CACGTG 1 cut(s) 971
EcoRII CCWGG 2 cut(s) 691, 1092
EcoT14I CCWWGG 1 cut(s) 1122
EcoT38I GRGCYC 1 cut(s) 1155
ErhI CCWWGG 1 cut(s) 1122
FaeI CATG 7 cut(s) 134, 185, 277, 451, 631, 838, 1081
FaqI GGGAC 2 cut(s) 126, 699
FatI CATG 7 cut(s) 130, 181, 273, 447, 627, 834, 1077
FauI CCCGC 1 cut(s) 1116
FauNDI CATATG 1 cut(s) 711
Fnu4HI GCNGC 4 cut(s) 144, 195, 1088, 1174
FokI GGATG 3 cut(s) 121, 293, 647
FriOI GRGCYC 1 cut(s) 1155
Fsp4HI GCNGC 4 cut(s) 144, 195, 1088, 1174
FspBI CTAG 2 cut(s) 666, 674
GlaI GCGC 1 cut(s) 1091
GluI GCNGC 4 cut(s) 144, 195, 1088, 1174
GsuI CTGGAG 1 cut(s) 998
HaeIII GGCC 6 cut(s) 25, 38, 54, 699, 967, 1167
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 20
HhaI GCGC 1 cut(s) 1092
Hin1I GRCGYC 1 cut(s) 688
Hin1II CATG 7 cut(s) 134, 185, 277, 451, 631, 838, 1081
Hin6I GCGC 1 cut(s) 1090
HinP1I GCGC 1 cut(s) 1090
HincII GTYRAC 5 cut(s) 232, 445, 478, 574, 832
HindII GTYRAC 5 cut(s) 232, 445, 478, 574, 832
HinfI GANTC 4 cut(s) 458, 845, 1031, 1161
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 362, 1108
Hpy188I TCNGA 8 cut(s) 64, 91, 280, 377, 634, 681, 764, 842
Hpy188III TCNNGA 1 cut(s) 99
Hpy99I CGWCG 1 cut(s) 1173
HpyAV CCTTC 4 cut(s) 43, 520, 676, 907
HpyCH4III ACNGT 7 cut(s) 295, 421, 523, 649, 808, 910, 950
HpyCH4IV ACGT 2 cut(s) 688, 970
HpyCH4V TGCA 6 cut(s) 328, 430, 715, 817, 1050, 1062
HpyF10VI GCNNNNNNNGC 7 cut(s) 509, 705, 735, 896, 997, 1173, 1182
HpyF3I CTNAG 5 cut(s) 88, 312, 504, 891, 1008
HpySE526I ACGT 2 cut(s) 688, 970
Hsp92I GRCGYC 1 cut(s) 688
Hsp92II CATG 7 cut(s) 134, 185, 277, 451, 631, 838, 1081
HspAI GCGC 1 cut(s) 1090
Kzo9I GATC 3 cut(s) 82, 388, 775
LmnI GCTCC 4 cut(s) 151, 267, 621, 1084
Lsp1109I GCAGC 4 cut(s) 130, 181, 1074, 1160
LweI GCATC 6 cut(s) 115, 173, 224, 566, 579, 586
MaeI CTAG 2 cut(s) 666, 674
MaeII ACGT 2 cut(s) 688, 970
MaeIII GTNAC 5 cut(s) 283, 421, 637, 808, 1037
MalI GATC 3 cut(s) 84, 390, 777
MbiI CCGCTC 1 cut(s) 1111
MboI GATC 3 cut(s) 82, 388, 775
MboII GAAGA 7 cut(s) 51, 55, 58, 114, 364, 542, 929
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 1155
MluCI AATT 6 cut(s) 156, 329, 716, 941, 1021, 1064
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 4 cut(s) 202, 258, 612, 659
MslI CAYNNNNRTG 2 cut(s) 135, 186
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 693, 1094
MvaI CCWGG 2 cut(s) 693, 1094
MvnI CGCG 2 cut(s) 77, 100
MwoI GCNNNNNNNGC 7 cut(s) 509, 705, 735, 896, 997, 1173, 1182
NdeI CATATG 1 cut(s) 711
NdeII GATC 3 cut(s) 82, 388, 775
NlaIII CATG 7 cut(s) 134, 185, 277, 451, 631, 838, 1081
NlaIV GGNNCC 1 cut(s) 966
NmuCI GTSAC 2 cut(s) 283, 637
NruI TCGCGA 1 cut(s) 100
NspI RCATGY 4 cut(s) 277, 451, 631, 838
PciI ACATGT 4 cut(s) 273, 447, 627, 834
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PfeI GAWTC 3 cut(s) 458, 845, 1161
PkrI GCNGC 4 cut(s) 145, 196, 1089, 1175
PleI GAGTC 1 cut(s) 1025
PmaCI CACGTG 1 cut(s) 971
PmlI CACGTG 1 cut(s) 971
PpsI GAGTC 1 cut(s) 1025
Ppu21I YACGTR 1 cut(s) 971
PscI ACATGT 4 cut(s) 273, 447, 627, 834
PshAI GACNNNNGTC 1 cut(s) 1124
Psp6I CCWGG 2 cut(s) 691, 1092
PspCI CACGTG 1 cut(s) 971
PspGI CCWGG 2 cut(s) 691, 1092
PspN4I GGNNCC 1 cut(s) 966
PspPI GGNCC 5 cut(s) 23, 37, 52, 698, 965
PsuI RGATCY 1 cut(s) 82
RruI TCGCGA 1 cut(s) 100
RsaI GTAC 2 cut(s) 664, 986
RsaNI GTAC 2 cut(s) 663, 985
RseI CAYNNNNRTG 2 cut(s) 135, 186
SatI GCNGC 4 cut(s) 144, 195, 1088, 1174
Sau3AI GATC 3 cut(s) 82, 388, 775
Sau96I GGNCC 5 cut(s) 23, 37, 52, 698, 965
ScaI AGTACT 1 cut(s) 664
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 2 cut(s) 693, 1094
SduI GDGCHC 1 cut(s) 1155
SfaNI GCATC 6 cut(s) 115, 173, 224, 566, 579, 586
SmiMI CAYNNNNRTG 2 cut(s) 135, 186
Sse9I AATT 6 cut(s) 156, 329, 716, 941, 1021, 1064
SsiI CCGC 6 cut(s) 75, 135, 265, 619, 1102, 1109
SspI AATATT 1 cut(s) 1136
SspMI CTAG 2 cut(s) 666, 674
StyD4I CCNGG 2 cut(s) 691, 1092
StyI CCWWGG 1 cut(s) 1122
TaaI ACNGT 7 cut(s) 295, 421, 523, 649, 808, 910, 950
TaiI ACGT 2 cut(s) 691, 973
TaqI TCGA 2 cut(s) 703, 864
TasI AATT 6 cut(s) 156, 329, 716, 941, 1021, 1064
TatI WGTACW 1 cut(s) 662
TfiI GAWTC 3 cut(s) 458, 845, 1161
TscAI CASTG 5 cut(s) 298, 496, 652, 883, 1064
TseFI GTSAC 2 cut(s) 283, 637
TseI GCWGC 4 cut(s) 143, 194, 1087, 1173
Tsp45I GTSAC 2 cut(s) 283, 637
TspDTI ATGAA 5 cut(s) 369, 419, 756, 806, 882
TspRI CASTG 5 cut(s) 298, 496, 652, 883, 1064
XapI RAATTY 1 cut(s) 1064
XceI RCATGY 4 cut(s) 277, 451, 631, 838
XspI CTAG 2 cut(s) 666, 674
ZraI GACGTC 1 cut(s) 689
ZrmI AGTACT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.