Rroxscaffold_5G00383220

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0018178
Physical Location & Seq
Reverse (-)
62876172 .. 62879935
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383220.1

Sequence Viewer

Length: 1332 bp
ATGGCGAAGCAGCCACCCATAGGGCCAAAGCGTCCGGCCCAGAACAAGAAGGGCCATTCTTCCGATTGGGTCTCCGAGACAGTTCCCTCAGACGGCTCGCGAAGATGGATGGGAGCTGGCCCATTCGATCAGATTGGAAATGCTCATGGCGGTGTTGCTGCTCCAAACCCAATTCAAGCATCGTTTGGAAATGAGCATGGTGGTGTTGCTGCTACAAACCCAATTCAAGCATCGCTTGGAAATGCTCATGGAGAAGGCTCCGGCGGTGTTGCTGCTACAAGTACAAACCCAATTCAAGCATCGTTTGGAAATGTCAACAAAGAAAATGAGATCCGTGATGCCACAAAGGTCGAAAAGCTCCGCCCAGACATGTCGGATGTCACTCTCACTGTAAAGGTTGTTAGTAATAAGAGAGTAGGTCGGAAGGGACGTCCTGGTGGCCCTCGAAGCCATAAGCAATTTGCTGACTGCGTGGTTGGCGATGAAACTGGAGTGATAATCTTCAGAGCTAAAAACGATCAAGTGGACTTGATGAAAGTGGGTTTGACTGTAACTCTGCAAAATGCCAAAGTTGACATGTTCAATGATTCAATGAGGCTTGTTGTCGATGAAAGTGGCAGTGTTCAAGTTGCTAAGCCTGCTGGTTTTACTGTGAAGGAAGATCGCAACCTCTCACTCATTCAAGCATCATTTGGAAATGTCAACAAAGACAATGAGATGCGTGATGCCACAAAGGTCGAAAAGCTCCGCCCAGACATGTCGGATGTCACTCTCACTGTAAAGGTTGTTAGTAATAAGATAGTAGGTCGGAAGGGACGTCCTGGTGGCCCTCGAAGCCATAAGCAATTTGCTGACTGCATGGTTGGCGATGAAACTGGAATGATAAACTTCAGAGCTAAAAACGATCAAGTGGACTTGATGAAAGAGGGTTTGACTGTAACTCTGCAAAATGCCAAAGTTGACATGTTCAGAGATTCAATGAGGCTTGTTGTCGATGAAAGTGGCAGTGTTGAAGTTGCTGAGCCTGCCGGTTTTACTGTGAAGGAAGATAGCAACCTCTCACTCATTCAATTTGACCGTGTTCAAAAAAAGGAGGGGCCACGTGAACTGGAGGTGTACGAGCAGGGTATGCCCAAGACTGAGCTTTGCTTGAATTGGCAGGACTCTGGTTACTGCCGTTGGAAAGACCACTGCAAATTTTCTCACGGCATGGAGGAGCTGCGCCCGGTGAATCGCCACCCGCTCTACAAGACCAAGGTCTGCCAATATTGGCTTGCCGATGAGCCCTGTGGATTCGGCCATCGCTGCCATTTTCGCCACACACTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

443

Amino Acids

48.66

Weight (kDa)

8.76

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 120 - 210 2.4e-30 Single-stranded DNA binding protein Ssb-like, OB fold
OB_Ssb-like PF21473 249 - 339 1.8e-30 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018178)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0442351 RchiOBHm_Chr7g0219251
rosa_laevigata RLG00000006016
rosa_multiflora Rmu_co8418355.1_g000001 Rmu_sc0000124.1_g000049
rosa_roxburghii Rroxscaffold_5G00383220
rosa_rugosa Rorug04G0340500
rosa_samantha Rh4AG392200 Rh4BG405700 Rh4CG420800 Rh4DG398900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 433, 820
AccBSI CCGCTC 1 cut(s) 1243
AccII CGCG 1 cut(s) 100
AciI CCGC 5 cut(s) 150, 264, 361, 748, 1241
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 1297
AcsI RAATTY 1 cut(s) 1196
AcuI CTGAAG 2 cut(s) 487, 874
AcvI CACGTG 1 cut(s) 1103
AcyI GRCGYC 2 cut(s) 430, 817
AfaI GTAC 2 cut(s) 283, 1118
AfiI CCNNNNNNNGG 2 cut(s) 20, 92
AflIII ACRYGT 4 cut(s) 369, 576, 756, 963
AjnI CCWGG 2 cut(s) 433, 820
AluBI AGCT 7 cut(s) 116, 358, 509, 745, 896, 1144, 1219
AluI AGCT 7 cut(s) 116, 358, 509, 745, 896, 1144, 1219
Alw26I GTCTC 2 cut(s) 71, 76
AlwI GGATC 1 cut(s) 325
AoxI GGCC 8 cut(s) 23, 36, 52, 118, 439, 826, 1097, 1297
ApeKI GCWGC 6 cut(s) 10, 158, 209, 272, 1219, 1305
ApoI RAATTY 1 cut(s) 1196
AspLEI GCGC 1 cut(s) 1224
AspS9I GGNCC 7 cut(s) 23, 37, 52, 119, 440, 827, 1097
AsuC2I CCSGG 1 cut(s) 1226
AsuHPI GGTGA 1 cut(s) 1240
BanII GRGCYC 1 cut(s) 1287
BbrPI CACGTG 1 cut(s) 1103
BbvI GCAGC 6 cut(s) 22, 145, 196, 259, 1206, 1292
BccI CCATC 3 cut(s) 99, 103, 1308
BceAI ACGGC 3 cut(s) 109, 1161, 1222
BciT130I CCWGG 2 cut(s) 435, 822
BcnI CCSGG 1 cut(s) 1226
BcoDI GTCTC 2 cut(s) 71, 76
BisI GCNGC 6 cut(s) 11, 159, 210, 273, 1220, 1306
BlpI GCTNAGC 2 cut(s) 633, 1020
BlsI GCNGC 6 cut(s) 12, 160, 211, 274, 1221, 1307
Bme1390I CCNGG 3 cut(s) 435, 822, 1226
BmgT120I GGNCC 7 cut(s) 23, 37, 52, 119, 440, 827, 1097
BmiI GGNNCC 2 cut(s) 259, 1098
BmrFI CCNGG 3 cut(s) 435, 822, 1226
BmsI GCATC 7 cut(s) 188, 239, 308, 328, 695, 708, 715
BoxI GACNNNNGTC 1 cut(s) 1256
BpmI CTGGAG 2 cut(s) 510, 1130
Bpu1102I GCTNAGC 2 cut(s) 633, 1020
BpuMI CCSGG 1 cut(s) 1226
BsaAI YACGTR 1 cut(s) 1103
BsaHI GRCGYC 2 cut(s) 430, 817
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 1254
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 92
Bse118I RCCGGY 1 cut(s) 1028
Bse1I ACTGG 3 cut(s) 493, 880, 1113
BseBI CCWGG 2 cut(s) 435, 822
BseDI CCNNGG 1 cut(s) 1254
BseGI GGATG 3 cut(s) 114, 382, 769
BseLI CCNNNNNNNGG 2 cut(s) 20, 92
BseMII CTCAG 3 cut(s) 102, 1011, 1131
BseNI ACTGG 3 cut(s) 493, 880, 1113
BseRI GAGGAG 1 cut(s) 1229
BseXI GCAGC 6 cut(s) 22, 145, 196, 259, 1206, 1292
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 8 cut(s) 25, 38, 54, 120, 441, 828, 1099, 1299
BsiSI CCGG 4 cut(s) 35, 261, 1029, 1226
BslFI GGGAC 2 cut(s) 441, 828
BslI CCNNNNNNNGG 2 cut(s) 20, 92
BsmAI GTCTC 2 cut(s) 71, 76
BsmFI GGGAC 2 cut(s) 441, 828
BsnI GGCC 8 cut(s) 25, 38, 54, 120, 441, 828, 1099, 1299
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 1287
Bsp143I GATC 5 cut(s) 127, 330, 517, 661, 904
Bsp1720I GCTNAGC 2 cut(s) 633, 1020
Bsp68I TCGCGA 1 cut(s) 100
BspACI CCGC 5 cut(s) 150, 264, 361, 748, 1241
BspANI GGCC 8 cut(s) 25, 38, 54, 120, 441, 828, 1099, 1299
BspCNI CTCAG 3 cut(s) 101, 1012, 1132
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 259, 1098
BspPI GGATC 1 cut(s) 325
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 1243
BsrFI RCCGGY 1 cut(s) 1028
BsrI ACTGG 3 cut(s) 493, 880, 1113
BssAI RCCGGY 1 cut(s) 1028
BssECI CCNNGG 1 cut(s) 1254
BssMI GATC 5 cut(s) 127, 330, 517, 661, 904
BssNI GRCGYC 2 cut(s) 430, 817
BssT1I CCWWGG 1 cut(s) 1254
Bst2UI CCWGG 2 cut(s) 435, 822
Bst4CI ACNGT 8 cut(s) 82, 391, 550, 652, 778, 937, 1039, 1079
BstACI GRCGYC 2 cut(s) 430, 817
BstAPI GCANNNNNTGC 1 cut(s) 1129
BstBAI YACGTR 1 cut(s) 1103
BstC8I GCNNGC 5 cut(s) 98, 118, 639, 1026, 1275
BstDEI CTNAG 4 cut(s) 88, 633, 1020, 1140
BstF5I GGATG 3 cut(s) 114, 382, 769
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 1224
BstKTI GATC 5 cut(s) 130, 333, 520, 664, 907
BstMAI GTCTC 2 cut(s) 71, 76
BstMBI GATC 5 cut(s) 127, 330, 517, 661, 904
BstMWI GCNNNNNNNGC 9 cut(s) 447, 477, 638, 834, 864, 1025, 1129, 1305, 1314
BstNI CCWGG 2 cut(s) 435, 822
BstNSI RCATGY 4 cut(s) 373, 580, 760, 967
BstPAI GACNNNNGTC 1 cut(s) 1256
BstSCI CCNGG 3 cut(s) 433, 820, 1224
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 6 cut(s) 22, 145, 196, 259, 1206, 1292
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 8 cut(s) 25, 38, 54, 120, 441, 828, 1099, 1299
BtgZI GCGATG 4 cut(s) 216, 495, 882, 1286
BtsCI GGATG 3 cut(s) 114, 382, 769
BtsI GCAGTG 3 cut(s) 625, 1012, 1189
BtsIMutI CAGTG 5 cut(s) 387, 625, 774, 1012, 1189
BtuMI TCGCGA 1 cut(s) 100
Cac8I GCNNGC 5 cut(s) 98, 118, 639, 1026, 1275
CfoI GCGC 1 cut(s) 1224
Cfr10I RCCGGY 1 cut(s) 1028
Cfr13I GGNCC 7 cut(s) 23, 37, 52, 119, 440, 827, 1097
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 282, 1117
CviAII CATG 9 cut(s) 146, 197, 248, 370, 577, 757, 859, 964, 1210
CviQI GTAC 2 cut(s) 282, 1117
DdeI CTNAG 4 cut(s) 88, 633, 1020, 1140
DpnI GATC 5 cut(s) 129, 332, 519, 663, 906
DpnII GATC 5 cut(s) 127, 330, 517, 661, 904
EaeI YGGCCR 1 cut(s) 1297
EciI GGCGGA 2 cut(s) 350, 737
Eco130I CCWWGG 1 cut(s) 1254
Eco24I GRGCYC 1 cut(s) 1287
Eco31I GGTCTC 1 cut(s) 76
Eco57I CTGAAG 2 cut(s) 487, 874
Eco72I CACGTG 1 cut(s) 1103
EcoRII CCWGG 2 cut(s) 433, 820
EcoT14I CCWWGG 1 cut(s) 1254
EcoT38I GRGCYC 1 cut(s) 1287
ErhI CCWWGG 1 cut(s) 1254
FaeI CATG 9 cut(s) 149, 200, 251, 373, 580, 760, 862, 967, 1213
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 2 cut(s) 441, 828
FatI CATG 9 cut(s) 145, 196, 247, 369, 576, 756, 858, 963, 1209
FauI CCCGC 1 cut(s) 1248
Fnu4HI GCNGC 6 cut(s) 11, 159, 210, 273, 1220, 1306
FokI GGATG 3 cut(s) 121, 389, 776
FriOI GRGCYC 1 cut(s) 1287
Fsp4HI GCNGC 6 cut(s) 11, 159, 210, 273, 1220, 1306
GlaI GCGC 1 cut(s) 1223
GluI GCNGC 6 cut(s) 11, 159, 210, 273, 1220, 1306
GsuI CTGGAG 2 cut(s) 510, 1130
HaeIII GGCC 8 cut(s) 25, 38, 54, 120, 441, 828, 1099, 1299
HapII CCGG 4 cut(s) 35, 261, 1029, 1226
HgaI GACGC 1 cut(s) 20
HhaI GCGC 1 cut(s) 1224
Hin1I GRCGYC 2 cut(s) 430, 817
Hin1II CATG 9 cut(s) 149, 200, 251, 373, 580, 760, 862, 967, 1213
Hin6I GCGC 1 cut(s) 1222
HinP1I GCGC 1 cut(s) 1222
HincII GTYRAC 4 cut(s) 316, 574, 703, 961
HindII GTYRAC 4 cut(s) 316, 574, 703, 961
HinfI GANTC 5 cut(s) 587, 974, 1163, 1231, 1293
HpaII CCGG 4 cut(s) 35, 261, 1029, 1226
HphI GGTGA 1 cut(s) 1240
Hpy166II GTNNAC 8 cut(s) 316, 526, 574, 703, 913, 961, 1106, 1117
Hpy188III TCNNGA 1 cut(s) 99
Hpy8I GTNNAC 8 cut(s) 316, 526, 574, 703, 913, 961, 1106, 1117
HpyAV CCTTC 6 cut(s) 43, 248, 418, 649, 805, 1036
HpyCH4III ACNGT 8 cut(s) 82, 391, 550, 652, 778, 937, 1039, 1079
HpyCH4IV ACGT 3 cut(s) 430, 817, 1102
HpyCH4V TGCA 4 cut(s) 559, 858, 946, 1194
HpyF10VI GCNNNNNNNGC 9 cut(s) 447, 477, 638, 834, 864, 1025, 1129, 1305, 1314
HpyF3I CTNAG 4 cut(s) 88, 633, 1020, 1140
HpySE526I ACGT 3 cut(s) 430, 817, 1102
Hsp92I GRCGYC 2 cut(s) 430, 817
Hsp92II CATG 9 cut(s) 149, 200, 251, 373, 580, 760, 862, 967, 1213
HspAI GCGC 1 cut(s) 1222
Kzo9I GATC 5 cut(s) 127, 330, 517, 661, 904
LmnI GCTCC 6 cut(s) 113, 166, 263, 363, 750, 1216
Lsp1109I GCAGC 6 cut(s) 22, 145, 196, 259, 1206, 1292
LweI GCATC 7 cut(s) 188, 239, 308, 328, 695, 708, 715
MaeII ACGT 3 cut(s) 430, 817, 1102
MaeIII GTNAC 5 cut(s) 379, 550, 766, 937, 1169
MalI GATC 5 cut(s) 129, 332, 519, 663, 906
MbiI CCGCTC 1 cut(s) 1243
MboI GATC 5 cut(s) 127, 330, 517, 661, 904
MboII GAAGA 5 cut(s) 51, 114, 493, 671, 1058
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 1287
MluCI AATT 8 cut(s) 171, 222, 291, 458, 845, 1070, 1153, 1196
MlyI GAGTC 1 cut(s) 1157
MmeI TCCRAC 5 cut(s) 354, 401, 741, 788, 1160
MslI CAYNNNNRTG 2 cut(s) 150, 201
MspI CCGG 4 cut(s) 35, 261, 1029, 1226
MspR9I CCNGG 3 cut(s) 435, 822, 1226
MvaI CCWGG 2 cut(s) 435, 822
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 9 cut(s) 447, 477, 638, 834, 864, 1025, 1129, 1305, 1314
NciI CCSGG 1 cut(s) 1226
NdeII GATC 5 cut(s) 127, 330, 517, 661, 904
NlaIII CATG 9 cut(s) 149, 200, 251, 373, 580, 760, 862, 967, 1213
NlaIV GGNNCC 2 cut(s) 259, 1098
NmuCI GTSAC 2 cut(s) 379, 766
NruI TCGCGA 1 cut(s) 100
NspI RCATGY 4 cut(s) 373, 580, 760, 967
PciI ACATGT 4 cut(s) 369, 576, 756, 963
PcsI WCGNNNNNNNCGW 2 cut(s) 427, 814
PfeI GAWTC 4 cut(s) 587, 974, 1231, 1293
PkrI GCNGC 6 cut(s) 12, 160, 211, 274, 1221, 1307
PleI GAGTC 1 cut(s) 1157
PmaCI CACGTG 1 cut(s) 1103
PmlI CACGTG 1 cut(s) 1103
PpsI GAGTC 1 cut(s) 1157
Ppu21I YACGTR 1 cut(s) 1103
PscI ACATGT 4 cut(s) 369, 576, 756, 963
PshAI GACNNNNGTC 1 cut(s) 1256
Psp6I CCWGG 2 cut(s) 433, 820
PspCI CACGTG 1 cut(s) 1103
PspGI CCWGG 2 cut(s) 433, 820
PspN4I GGNNCC 2 cut(s) 259, 1098
PspPI GGNCC 7 cut(s) 23, 37, 52, 119, 440, 827, 1097
PsuI RGATCY 1 cut(s) 330
RruI TCGCGA 1 cut(s) 100
RsaI GTAC 2 cut(s) 283, 1118
RsaNI GTAC 2 cut(s) 282, 1117
RseI CAYNNNNRTG 2 cut(s) 150, 201
SatI GCNGC 6 cut(s) 11, 159, 210, 273, 1220, 1306
Sau3AI GATC 5 cut(s) 127, 330, 517, 661, 904
Sau96I GGNCC 7 cut(s) 23, 37, 52, 119, 440, 827, 1097
SchI GAGTC 1 cut(s) 1157
ScrFI CCNGG 3 cut(s) 435, 822, 1226
SduI GDGCHC 1 cut(s) 1287
SfaNI GCATC 7 cut(s) 188, 239, 308, 328, 695, 708, 715
SmiMI CAYNNNNRTG 2 cut(s) 150, 201
Sse9I AATT 8 cut(s) 171, 222, 291, 458, 845, 1070, 1153, 1196
SsiI CCGC 5 cut(s) 150, 264, 361, 748, 1241
SspI AATATT 1 cut(s) 1268
StyD4I CCNGG 3 cut(s) 433, 820, 1224
StyI CCWWGG 1 cut(s) 1254
TaaI ACNGT 8 cut(s) 82, 391, 550, 652, 778, 937, 1039, 1079
TaiI ACGT 3 cut(s) 433, 820, 1105
TaqI TCGA 7 cut(s) 126, 351, 445, 606, 738, 832, 993
TasI AATT 8 cut(s) 171, 222, 291, 458, 845, 1070, 1153, 1196
TatI WGTACW 1 cut(s) 281
TfiI GAWTC 4 cut(s) 587, 974, 1231, 1293
TscAI CASTG 5 cut(s) 394, 625, 781, 1012, 1196
TseFI GTSAC 2 cut(s) 379, 766
TseI GCWGC 6 cut(s) 10, 158, 209, 272, 1219, 1305
Tsp45I GTSAC 2 cut(s) 379, 766
TspDTI ATGAA 6 cut(s) 498, 548, 624, 885, 935, 1011
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 5 cut(s) 394, 625, 781, 1012, 1196
XapI RAATTY 1 cut(s) 1196
XceI RCATGY 4 cut(s) 373, 580, 760, 967
ZraI GACGTC 2 cut(s) 431, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.