Rorug04G0340500

ribonuclease H protein At1g65750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003500
Physical Location & Seq
Reverse (-)
51356436 .. 51361265
4830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0340500.1

Sequence Viewer

Length: 1026 bp
ATGGACCCTGCCTCTCGCCCCGCAGTCGTCATCGACAACGGCACCGGGTATACTAAGATGGGGTTCGCCGGAAACGTCGAGCCGTGTTACATTGTCCCCACTGTAGTAGCTGTCAACGAATCGTTTCTGAACCAATCGAGAAGCACCTCCAAGGCGAGCTGGGTAGCGCAGCACAATGCGGGTGTGATGGCTGATCTTGATTTCTTCATAGGGGATGAGGCTTTGGAGAAGTCTCGATCCAGCTCTACTTATAATTTGAGCTATCCGATTAGTCATGGGCAGGTGGACAATTGGGATTCAATGGAGCGGTTTTGGCAGCAGTGTATATTTAACTATTTGCGTTGTGATCCTGAAGATCATTACTTTCTTTTGACTGAGAGCCCGCTTACTGCCCCGGAGAGCCGGGAGTACACCGGAGAAATTATGTTTGAGACTTTCAATGTTCCTGGCCTTTACATTGCTGTGAATTCGGTACTTGCTCTCGCAGCTGGCTACACTACCTCTAAGTGTGAGATGACAGGTGTTGTAGTAGATGTTGGAGATGGGGCAAGTCATGTTGTTCCTGTTGCAGAAGGTTATGTGATTGGGAGCAGCATCAAGTCAGTTCCGATTGCCGGGAGAGATGTCACTCTCTTTGTTCAACAGCTTATGCGGGAAAGAGGAGAGAACGTGCCACCAGAAGACTCCTTTGATGTAGCTCGCAAAGTGAAGGAAATGTATTGCTATACTAGTTCTGACATTGTCAAGGAGTTCAATAAGCACGATAAAGAACCATCCAAATATATTAAGCAATGGAAAGGCATTAAACCAAAGACAGGGGCACCCTACACTTGTGATATTGGCTATGAACGATTTCTCGGACCAGAGGTATTTTTTAATCCTGAGATTTATAGCAGCGATTTCACGACTCCATTACCAGTTGTAATTGACAAGTGTATTCAGTCTGCCCCAATTGACACCAGGAGGTCTTTGTATAAGTTCCAGTTGGCATTTATCTCCTCCTCTGTGCCCATGTCTGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

341

Amino Acids

37.89

Weight (kDa)

4.96

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 7 - 228 5.9e-50 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
AarI CACCTGC 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 271
AccB1I GGYRCC 2 cut(s) 41, 820
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 50
AciI CCGC 5 cut(s) 21, 179, 307, 383, 652
AclWI GGATC 2 cut(s) 231, 341
AcsI RAATTY 1 cut(s) 466
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 2 cut(s) 410, 474
AfiI CCNNNNNNNGG 3 cut(s) 614, 815, 1016
AgsI TTSAA 4 cut(s) 300, 439, 641, 754
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 2 cut(s) 445, 959
AluBI AGCT 7 cut(s) 110, 159, 243, 261, 488, 646, 698
AluI AGCT 7 cut(s) 110, 159, 243, 261, 488, 646, 698
Alw26I GTCTC 2 cut(s) 237, 425
AlwI GGATC 2 cut(s) 231, 341
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 5 cut(s) 169, 316, 485, 591, 894
ApoI RAATTY 1 cut(s) 466
Asp700I GAANNNNTTC 2 cut(s) 123, 852
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 2 cut(s) 4, 860
AsuC2I CCSGG 4 cut(s) 46, 395, 404, 616
AvaII GGWCC 2 cut(s) 4, 860
BaeGI GKGCMC 2 cut(s) 823, 1011
BanI GGYRCC 2 cut(s) 41, 820
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 687
BbvI GCAGC 5 cut(s) 181, 328, 497, 603, 906
BccI CCATC 4 cut(s) 52, 181, 536, 781
BceAI ACGGC 2 cut(s) 55, 67
BciT130I CCWGG 2 cut(s) 447, 961
BcnI CCSGG 4 cut(s) 46, 395, 404, 616
BcoDI GTCTC 2 cut(s) 237, 425
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 5 cut(s) 170, 317, 486, 592, 895
BlsI GCNGC 5 cut(s) 171, 318, 487, 593, 896
Bme1390I CCNGG 6 cut(s) 46, 395, 404, 447, 616, 961
Bme18I GGWCC 2 cut(s) 4, 860
BmgT120I GGNCC 2 cut(s) 4, 860
BmiI GGNNCC 3 cut(s) 6, 43, 822
BmrFI CCNGG 6 cut(s) 46, 395, 404, 447, 616, 961
BmsI GCATC 1 cut(s) 603
BpiI GAAGAC 1 cut(s) 687
BpuMI CCSGG 4 cut(s) 46, 395, 404, 616
BsaJI CCNNGG 2 cut(s) 150, 393
BsaWI WCCGGW 1 cut(s) 413
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 3 cut(s) 614, 815, 1016
Bse1I ACTGG 2 cut(s) 917, 982
Bse3DI GCAATG 2 cut(s) 456, 797
BseBI CCWGG 2 cut(s) 447, 961
BseDI CCNNGG 2 cut(s) 150, 393
BseGI GGATG 2 cut(s) 220, 773
BseLI CCNNNNNNNGG 3 cut(s) 614, 815, 1016
BseMI GCAATG 2 cut(s) 456, 797
BseMII CTCAG 2 cut(s) 366, 873
BseNI ACTGG 2 cut(s) 917, 982
BseRI GAGGAG 3 cut(s) 675, 988, 991
BseSI GKGCMC 2 cut(s) 823, 1011
BseXI GCAGC 5 cut(s) 181, 328, 497, 603, 906
BseYI CCCAGC 1 cut(s) 159
BshFI GGCC 1 cut(s) 450
BshNI GGYRCC 2 cut(s) 41, 820
BsiSI CCGG 6 cut(s) 45, 69, 395, 403, 414, 615
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 614, 815, 1016
BsmAI GTCTC 2 cut(s) 237, 425
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 450
Bsp1286I GDGCHC 3 cut(s) 383, 823, 1011
Bsp143I GATC 4 cut(s) 193, 236, 346, 355
BspACI CCGC 5 cut(s) 21, 179, 307, 383, 652
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 2 cut(s) 367, 874
BspLI GGNNCC 3 cut(s) 6, 43, 822
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 2 cut(s) 231, 341
BspT107I GGYRCC 2 cut(s) 41, 820
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 456, 797
BsrI ACTGG 2 cut(s) 917, 982
BssECI CCNNGG 2 cut(s) 150, 393
BssMI GATC 4 cut(s) 193, 236, 346, 355
BssNAI GTATAC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 447, 961
Bst4CI ACNGT 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 157, 383, 490, 700
BstDEI CTNAG 4 cut(s) 54, 375, 504, 882
BstF5I GGATG 2 cut(s) 220, 773
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 4 cut(s) 196, 239, 349, 358
BstMAI GTCTC 2 cut(s) 237, 425
BstMBI GATC 4 cut(s) 193, 236, 346, 355
BstMWI GCNNNNNNNGC 2 cut(s) 313, 485
BstNI CCWGG 2 cut(s) 447, 961
BstSCI CCNGG 6 cut(s) 44, 393, 402, 445, 614, 959
BstSFI CTRYAG 1 cut(s) 102
BstSLI GKGCMC 2 cut(s) 823, 1011
BstV1I GCAGC 5 cut(s) 181, 328, 497, 603, 906
BstV2I GAAGAC 1 cut(s) 687
BstZ17I GTATAC 1 cut(s) 51
BsuRI GGCC 1 cut(s) 450
BtsCI GGATG 2 cut(s) 220, 773
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 2 cut(s) 99, 326
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 4 cut(s) 157, 383, 490, 700
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 4, 860
Csp6I GTAC 2 cut(s) 409, 473
CviAII CATG 3 cut(s) 275, 554, 1012
CviQI GTAC 2 cut(s) 409, 473
DdeI CTNAG 4 cut(s) 54, 375, 504, 882
DpnI GATC 4 cut(s) 195, 238, 348, 357
DpnII GATC 4 cut(s) 193, 236, 346, 355
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 4, 860
Eco57I CTGAAG 1 cut(s) 372
EcoRI GAATTC 1 cut(s) 466
EcoRII CCWGG 2 cut(s) 445, 959
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 278, 557, 1015
FaqI GGGAC 1 cut(s) 80
FatI CATG 3 cut(s) 274, 553, 1011
FauI CCCGC 4 cut(s) 28, 172, 390, 645
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 5 cut(s) 170, 317, 486, 592, 895
FokI GGATG 2 cut(s) 227, 760
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 5 cut(s) 170, 317, 486, 592, 895
FspBI CTAG 1 cut(s) 729
GlaI GCGC 1 cut(s) 168
GluI GCNGC 5 cut(s) 170, 317, 486, 592, 895
GsaI CCCAGC 1 cut(s) 163
HaeIII GGCC 1 cut(s) 450
HapII CCGG 6 cut(s) 45, 69, 395, 403, 414, 615
HhaI GCGC 1 cut(s) 169
Hin1II CATG 3 cut(s) 278, 557, 1015
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 4 cut(s) 119, 296, 683, 907
HpaII CCGG 6 cut(s) 45, 69, 395, 403, 414, 615
Hpy166II GTNNAC 4 cut(s) 51, 115, 286, 411
Hpy188I TCNGA 5 cut(s) 129, 267, 609, 736, 860
Hpy188III TCNNGA 6 cut(s) 138, 197, 234, 350, 881, 904
Hpy8I GTNNAC 4 cut(s) 51, 115, 286, 411
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 566, 703
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 2 cut(s) 75, 669
HpyCH4V TGCA 1 cut(s) 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 313, 485
HpyF3I CTNAG 4 cut(s) 54, 375, 504, 882
HpySE526I ACGT 2 cut(s) 75, 669
Hsp92II CATG 3 cut(s) 278, 557, 1015
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 4 cut(s) 193, 236, 346, 355
LmnI GCTCC 2 cut(s) 304, 588
Lsp1109I GCAGC 5 cut(s) 181, 328, 497, 603, 906
LweI GCATC 1 cut(s) 603
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 75, 669
MaeIII GTNAC 2 cut(s) 86, 625
MalI GATC 4 cut(s) 195, 238, 348, 357
MbiI CCGCTC 1 cut(s) 307
MboI GATC 4 cut(s) 193, 236, 346, 355
MboII GAAGA 3 cut(s) 196, 365, 692
MfeI CAATTG 2 cut(s) 289, 951
MhlI GDGCHC 3 cut(s) 383, 823, 1011
MluCI AATT 6 cut(s) 253, 289, 420, 466, 924, 951
MlyI GAGTC 2 cut(s) 677, 901
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 9 cut(s) 22, 157, 211, 511, 653, 859, 957, 1009, 1012
MroXI GAANNNNTTC 2 cut(s) 123, 852
MseI TTAA 4 cut(s) 330, 786, 804, 876
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 6 cut(s) 45, 69, 395, 403, 414, 615
MspR9I CCNGG 6 cut(s) 46, 395, 404, 447, 616, 961
MunI CAATTG 2 cut(s) 289, 951
MvaI CCWGG 2 cut(s) 447, 961
MwoI GCNNNNNNNGC 2 cut(s) 313, 485
NciI CCSGG 4 cut(s) 46, 395, 404, 616
NdeII GATC 4 cut(s) 193, 236, 346, 355
NlaIII CATG 3 cut(s) 278, 557, 1015
NlaIV GGNNCC 3 cut(s) 6, 43, 822
NmuCI GTSAC 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 123, 852
PfeI GAWTC 2 cut(s) 119, 296
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 5 cut(s) 171, 318, 487, 593, 896
PleI GAGTC 2 cut(s) 677, 901
PpsI GAGTC 2 cut(s) 677, 901
PsiI TTATAA 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 445, 959
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 2 cut(s) 445, 959
PspN4I GGNNCC 3 cut(s) 6, 43, 822
PspPI GGNCC 2 cut(s) 4, 860
PsyI GACNNNGTC 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 2 cut(s) 410, 474
RsaNI GTAC 2 cut(s) 409, 473
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 4 cut(s) 330, 786, 804, 876
SatI GCNGC 5 cut(s) 170, 317, 486, 592, 895
Sau3AI GATC 4 cut(s) 193, 236, 346, 355
Sau96I GGNCC 2 cut(s) 4, 860
SchI GAGTC 2 cut(s) 677, 901
ScrFI CCNGG 6 cut(s) 46, 395, 404, 447, 616, 961
SduI GDGCHC 3 cut(s) 383, 823, 1011
SfaNI GCATC 1 cut(s) 603
SfcI CTRYAG 1 cut(s) 102
SinI GGWCC 2 cut(s) 4, 860
SmiMI CAYNNNNRTG 1 cut(s) 461
SpeI ACTAGT 1 cut(s) 728
Sse9I AATT 6 cut(s) 253, 289, 420, 466, 924, 951
SsiI CCGC 5 cut(s) 21, 179, 307, 383, 652
SspMI CTAG 1 cut(s) 729
StyD4I CCNGG 6 cut(s) 44, 393, 402, 445, 614, 959
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 2 cut(s) 78, 672
TaqI TCGA 4 cut(s) 33, 78, 137, 235
TasI AATT 6 cut(s) 253, 289, 420, 466, 924, 951
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 2 cut(s) 119, 296
Tru1I TTAA 4 cut(s) 330, 786, 804, 876
Tru9I TTAA 4 cut(s) 330, 786, 804, 876
TscAI CASTG 2 cut(s) 106, 326
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 5 cut(s) 169, 316, 485, 591, 894
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 2 cut(s) 196, 861
TspRI CASTG 2 cut(s) 106, 326
Tth111I GACNNNGTC 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 4, 860
XapI RAATTY 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 2 cut(s) 123, 852
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.