AT1G10590

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003500
Physical Location & Seq
Reverse (-)
3501988 .. 3503544
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10590.1

Sequence Viewer

Length: 420 bp
ATGGCTGAGGCAACGTCTGCTTTGAGGAAGCCTGTGTTCACAAAGGTTAATGAGTTGAGACCAGGAACCAATGGCCACTCTCTGAATGTGAAAGTTGTCAACACGAAGATGGTGATGCAGAGAGGAGGAGGTCGTCCCATGGGTCCTCAGTCACGTCAGATGCGGATTGCCGAATGCTTAGTTGGTGATGAGACTGGAATCATAATCTTTACTGCAAGAAATGACCAAGTGGATATGATGAAAGAAGGCAGTGTAGTGACTCTGCGTAATGCGAAGATCGACATGTACAAGGGTTCTATGAGGCTTGCAGTTGATAGATGGGGACGTGTTGAAGTTGCAGAGGAGCCCACAGACATCACCGTGAAGGATGATAACAATCTTTCGCTGATCGAGTATGAGCTTGTGAGCGTCGAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.45

Weight (kDa)

6.6

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 112 4.8e-44 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 163
AcoI YGGCCR 1 cut(s) 73
AfaI GTAC 1 cut(s) 287
AflIII ACRYGT 2 cut(s) 282, 325
AgsI TTSAA 1 cut(s) 332
AjiI CACGTC 2 cut(s) 155, 326
AjnI CCWGG 1 cut(s) 61
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AluBI AGCT 1 cut(s) 400
AluI AGCT 1 cut(s) 400
Alw26I GTCTC 2 cut(s) 52, 185
AoxI GGCC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 3 cut(s) 124, 197, 349
AvaII GGWCC 1 cut(s) 143
BalI TGGCCA 1 cut(s) 75
BanII GRGCYC 1 cut(s) 348
BbvCI CCTCAGC 1 cut(s) 6
BccI CCATC 2 cut(s) 103, 312
BciT130I CCWGG 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 52, 185
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 1 cut(s) 143
BmgBI CACGTC 2 cut(s) 155, 326
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 3 cut(s) 67, 144, 345
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 2 cut(s) 105, 150
Bpu10I CCTNAGC 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 1 cut(s) 138
Bse1I ACTGG 1 cut(s) 199
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 1 cut(s) 375
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 3 cut(s) 138, 141, 356
BshFI GGCC 1 cut(s) 75
BslFI GGGAC 2 cut(s) 120, 336
BsmAI GTCTC 2 cut(s) 52, 185
BsmFI GGGAC 2 cut(s) 120, 336
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 1 cut(s) 75
Bso31I GGTCTC 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 348
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 2 cut(s) 276, 387
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 1 cut(s) 163
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 67, 144, 345
BspTNI GGTCTC 1 cut(s) 52
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 2 cut(s) 276, 387
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 1 cut(s) 361
BstAPI GCANNNNNTGC 1 cut(s) 17
BstAUI TGTACA 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 6, 147, 178
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 373
BstKTI GATC 2 cut(s) 279, 390
BstMAI GTCTC 2 cut(s) 52, 185
BstMBI GATC 2 cut(s) 276, 387
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 1 cut(s) 61
BsuRI GGCC 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 138
BtrI CACGTC 2 cut(s) 155, 326
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 397
Csp6I GTAC 1 cut(s) 286
CviAII CATG 2 cut(s) 139, 283
CviJI RGCY 6 cut(s) 5, 31, 75, 304, 346, 400
CviKI_1 RGCY 6 cut(s) 5, 31, 75, 304, 346, 400
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 3 cut(s) 6, 147, 178
DpnI GATC 2 cut(s) 278, 389
DpnII GATC 2 cut(s) 276, 387
EaeI YGGCCR 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 2 cut(s) 142, 286
FaiI YATR 6 cut(s) 140, 203, 236, 284, 299, 396
FaqI GGGAC 2 cut(s) 120, 336
FatI CATG 2 cut(s) 138, 282
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 348
HaeIII GGCC 1 cut(s) 75
HgaI GACGC 1 cut(s) 397
Hin1II CATG 2 cut(s) 142, 286
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 2 cut(s) 198, 259
HphI GGTGA 3 cut(s) 124, 197, 349
Hpy166II GTNNAC 2 cut(s) 39, 100
Hpy188I TCNGA 2 cut(s) 84, 159
Hpy8I GTNNAC 2 cut(s) 39, 100
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 2 cut(s) 239, 358
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 3 cut(s) 14, 154, 325
HpyCH4V TGCA 4 cut(s) 118, 215, 308, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 3 cut(s) 6, 147, 178
HpySE526I ACGT 3 cut(s) 14, 154, 325
Hsp92II CATG 2 cut(s) 142, 286
Kzo9I GATC 2 cut(s) 276, 387
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 4 cut(s) 45, 48, 75, 180
LweI GCATC 2 cut(s) 105, 150
MaeII ACGT 3 cut(s) 14, 154, 325
MaeIII GTNAC 2 cut(s) 150, 256
MalI GATC 2 cut(s) 278, 389
MboI GATC 2 cut(s) 276, 387
MboII GAAGA 2 cut(s) 118, 286
MhlI GDGCHC 1 cut(s) 348
MlsI TGGCCA 1 cut(s) 75
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 7 cut(s) 18, 116, 119, 122, 156, 294, 334
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 2 cut(s) 107, 359
Msp20I TGGCCA 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 1 cut(s) 17
NcoI CCATGG 1 cut(s) 138
NdeII GATC 2 cut(s) 276, 387
NlaIII CATG 2 cut(s) 142, 286
NlaIV GGNNCC 3 cut(s) 67, 144, 345
NmuCI GTSAC 2 cut(s) 150, 256
NspI RCATGY 1 cut(s) 286
PciI ACATGT 1 cut(s) 282
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 1 cut(s) 198
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PpuMI RGGWCCY 1 cut(s) 143
PscI ACATGT 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 3 cut(s) 67, 144, 345
PspPI GGNCC 1 cut(s) 143
PspPPI RGGWCCY 1 cut(s) 143
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 2 cut(s) 107, 359
SaqAI TTAA 1 cut(s) 48
Sau3AI GATC 2 cut(s) 276, 387
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 1 cut(s) 348
SetI ASST 6 cut(s) 17, 48, 133, 157, 328, 402
SfaNI GCATC 2 cut(s) 105, 150
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 2 cut(s) 107, 359
SsiI CCGC 1 cut(s) 163
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 3 cut(s) 17, 157, 328
TaqI TCGA 3 cut(s) 279, 390, 411
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 2 cut(s) 150, 256
Tsp45I GTSAC 2 cut(s) 150, 256
TspDTI ATGAA 1 cut(s) 254
TspRI CASTG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 143
XceI RCATGY 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.