MD13G1092100.v1.1

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003500
Physical Location & Seq
Reverse (-)
6497101 .. 6500196
3096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1092100.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGCTGAAGCGAAACCAGGATTGAGGAAACCAGTATTCACCAAGGTTGCCCAGCTCCGCCCAGGTACTTCTGGGCACACTCTGACTGTAAAGGTTGTTCATACTAAGATGGTGTTACAGAAGGGTCGCGCCGATGACCGTCAAGCACGCCAAACGCGAATTGCTGAATGCTTGGTTGGTGATGAAACCGGATTGATTATCTTCACAGCTAGAAACGATCAAGTGGACTTGATGCAAGAAGGTTCTACCATAACCCTGCGGAATGCCAAGATTGACATGTTTAAAGGATCAATGAGACTTGCTGTGGACAAGTGGGGCCGTGTCGAAGTTGCTGAACCTGCCAGTTTCACAGTTAAGGAAGATAACAACCTCTCCCTGGTTGAATATGAACTTGTCCAAGTTTTTGAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.32

Weight (kDa)

8.79

Isoelectric Point (pI)

22.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 110 1.2e-45 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 346
AccII CGCG 2 cut(s) 129, 157
AciI CCGC 2 cut(s) 58, 259
AclWI GGATC 1 cut(s) 295
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 376
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 383, 407
AjnI CCWGG 3 cut(s) 16, 61, 375
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 2 cut(s) 55, 209
AluI AGCT 2 cut(s) 55, 209
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 295
AoxI GGCC 1 cut(s) 316
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 2 cut(s) 31, 191
BaeGI GKGCMC 1 cut(s) 78
BccI CCATC 1 cut(s) 103
BceAI ACGGC 1 cut(s) 303
BciT130I CCWGG 3 cut(s) 18, 63, 377
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 346
Bme1390I CCNGG 3 cut(s) 18, 63, 377
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 317
BmrFI CCNGG 3 cut(s) 18, 63, 377
BmsI GCATC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 42, 61, 375
BsaWI WCCGGW 1 cut(s) 188
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse1I ACTGG 2 cut(s) 32, 342
BseBI CCWGG 3 cut(s) 18, 63, 377
BseDI CCNNGG 3 cut(s) 42, 61, 375
BseLI CCNNNNNNNGG 1 cut(s) 376
BseNI ACTGG 2 cut(s) 32, 342
BseSI GKGCMC 1 cut(s) 78
BseYI CCCAGC 1 cut(s) 51
Bsh1236I CGCG 2 cut(s) 129, 157
BshFI GGCC 1 cut(s) 318
BsiSI CCGG 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 376
BsmAI GTCTC 1 cut(s) 289
BsmI GAATGC 2 cut(s) 173, 268
BsnI GGCC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 2 cut(s) 217, 287
BspACI CCGC 2 cut(s) 58, 259
BspANI GGCC 1 cut(s) 318
BspFNI CGCG 2 cut(s) 129, 157
BspLI GGNNCC 1 cut(s) 317
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 1 cut(s) 295
BsrI ACTGG 2 cut(s) 32, 342
BssECI CCNNGG 3 cut(s) 42, 61, 375
BssMI GATC 2 cut(s) 217, 287
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 3 cut(s) 18, 63, 377
Bst4CI ACNGT 4 cut(s) 88, 140, 352, 411
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 1 cut(s) 105
BstFNI CGCG 2 cut(s) 129, 157
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 220, 290
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 2 cut(s) 217, 287
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 3 cut(s) 18, 63, 377
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 3 cut(s) 16, 61, 375
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 2 cut(s) 129, 157
BsuRI GGCC 1 cut(s) 318
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 148
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 316
Csp6I GTAC 1 cut(s) 66
CviAII CATG 1 cut(s) 277
CviJI RGCY 4 cut(s) 5, 55, 209, 318
CviKI_1 RGCY 4 cut(s) 5, 55, 209, 318
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 1 cut(s) 105
DpnI GATC 2 cut(s) 219, 289
DpnII GATC 2 cut(s) 217, 287
DraI TTTAAA 1 cut(s) 283
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 27
EcoRII CCWGG 3 cut(s) 16, 61, 375
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 280
FaiI YATR 4 cut(s) 102, 251, 278, 387
FatI CATG 1 cut(s) 276
FspBI CTAG 1 cut(s) 210
GlaI GCGC 1 cut(s) 130
GsaI CCCAGC 1 cut(s) 55
HaeIII GGCC 1 cut(s) 318
HapII CCGG 1 cut(s) 189
HhaI GCGC 1 cut(s) 131
Hin1II CATG 1 cut(s) 280
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HpaII CCGG 1 cut(s) 189
HphI GGTGA 2 cut(s) 31, 191
Hpy166II GTNNAC 2 cut(s) 226, 307
Hpy188I TCNGA 1 cut(s) 84
Hpy8I GTNNAC 2 cut(s) 226, 307
HpyAV CCTTC 2 cut(s) 115, 233
HpyCH4III ACNGT 4 cut(s) 88, 140, 352, 411
HpyCH4V TGCA 1 cut(s) 235
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 1 cut(s) 105
Hsp92II CATG 1 cut(s) 280
HspAI GCGC 1 cut(s) 129
Kzo9I GATC 2 cut(s) 217, 287
LmnI GCTCC 1 cut(s) 60
LweI GCATC 1 cut(s) 222
MaeI CTAG 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 114
MalI GATC 2 cut(s) 219, 289
MboI GATC 2 cut(s) 217, 287
MboII GAAGA 2 cut(s) 193, 371
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 1 cut(s) 159
MnlI CCTC 2 cut(s) 18, 380
MseI TTAA 2 cut(s) 282, 354
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 3 cut(s) 18, 63, 377
Mva1269I GAATGC 2 cut(s) 173, 268
MvaI CCWGG 3 cut(s) 18, 63, 377
MvnI CGCG 2 cut(s) 129, 157
MwoI GCNNNNNNNGC 1 cut(s) 338
NdeII GATC 2 cut(s) 217, 287
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 317
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PctI GAATGC 2 cut(s) 173, 268
PscI ACATGT 1 cut(s) 276
Psp6I CCWGG 3 cut(s) 16, 61, 375
PspFI CCCAGC 1 cut(s) 51
PspGI CCWGG 3 cut(s) 16, 61, 375
PspN4I GGNNCC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 316
PsrI GAACNNNNNNTAC 2 cut(s) 81, 113
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
SaqAI TTAA 2 cut(s) 282, 354
Sau3AI GATC 2 cut(s) 217, 287
Sau96I GGNCC 1 cut(s) 316
ScrFI CCNGG 3 cut(s) 18, 63, 377
SduI GDGCHC 1 cut(s) 78
SetI ASST 8 cut(s) 48, 57, 67, 96, 211, 244, 340, 372
SfaNI GCATC 1 cut(s) 222
Sse9I AATT 1 cut(s) 159
SsiI CCGC 2 cut(s) 58, 259
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 16, 61, 375
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 4 cut(s) 88, 140, 352, 411
TaqI TCGA 1 cut(s) 324
TasI AATT 1 cut(s) 159
Tru1I TTAA 2 cut(s) 282, 354
Tru9I TTAA 2 cut(s) 282, 354
TspDTI ATGAA 3 cut(s) 89, 198, 402
XceI RCATGY 1 cut(s) 280
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.