RLG00000006017

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003500
Physical Location & Seq
Forward (+)
3546419 .. 3547332
914 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006017

Sequence Viewer

Length: 408 bp
ATGGCAGACTCGAAACAAGCACCGAGCAAACCAACCTTCACAAAGATTATGCAGCTCCGCCCAATGACCTCTGGTGTCACTCTCACTGTAAAGGTTGTTAGTACTAAGATAGTATGCCCGAAGGGACGTGCTGGTGGTCCTCGAGGCCAAATGCTAATTGCTGATTGCTTGGTTGGCGATGAAACCGCAATGATAATCTTCACAGCTAGAAACGATCAAGTGGACTTGATGAAAGTGGGTTCGACTGTAACCCTGCGAAATGCCAAAATTGACATGTTTAAAGGATCAATGAGGCTTGCTGTGGACAAAAGTGGCCGTATTGAAGTTGCTGAGCCTGCCAAGTTTACCGTGATGGAAAATAACAACCTCTCACTGATTGAATTTGAACGTGTCGATGTCGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

14.72

Weight (kDa)

9.47

Isoelectric Point (pI)

18.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 109 2.9e-39 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 141
AciI CCGC 2 cut(s) 58, 186
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 1 cut(s) 380
AfaI GTAC 1 cut(s) 103
AflIII ACRYGT 2 cut(s) 273, 388
AgsI TTSAA 3 cut(s) 323, 380, 386
AjiI CACGTC 1 cut(s) 128
AluBI AGCT 2 cut(s) 55, 206
AluI AGCT 2 cut(s) 55, 206
AlwI GGATC 1 cut(s) 292
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 2 cut(s) 145, 313
ApeKI GCWGC 1 cut(s) 52
ApoI RAATTY 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 141
AvaII GGWCC 1 cut(s) 137
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 346
BceAI ACGGC 1 cut(s) 300
BfaI CTAG 1 cut(s) 207
BisI GCNGC 1 cut(s) 53
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 1 cut(s) 54
BmcAI AGTACT 1 cut(s) 103
Bme18I GGWCC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 141
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 137
Bpu1102I GCTNAGC 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 195
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 64
BshFI GGCC 2 cut(s) 147, 315
BsiHKCI CYCGRG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 2 cut(s) 147, 315
BsoBI CYCGRG 1 cut(s) 141
Bsp143I GATC 2 cut(s) 214, 284
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 2 cut(s) 58, 186
BspANI GGCC 2 cut(s) 147, 315
BspCNI CTCAG 1 cut(s) 322
BspPI GGATC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 195
BssMI GATC 2 cut(s) 214, 284
Bst4CI ACNGT 3 cut(s) 88, 247, 349
BstC8I GCNNGC 2 cut(s) 297, 336
BstDEI CTNAG 2 cut(s) 105, 330
BstKTI GATC 2 cut(s) 217, 287
BstMBI GATC 2 cut(s) 214, 284
BstMWI GCNNNNNNNGC 2 cut(s) 174, 335
BstNSI RCATGY 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 64
BsuRI GGCC 2 cut(s) 147, 315
BtgZI GCGATG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 128
BtsIMutI CAGTG 2 cut(s) 84, 371
Cac8I GCNNGC 2 cut(s) 297, 336
Cfr13I GGNCC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 102
CviAII CATG 1 cut(s) 274
CviJI RGCY 6 cut(s) 55, 147, 206, 295, 315, 334
CviKI_1 RGCY 6 cut(s) 55, 147, 206, 295, 315, 334
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 2 cut(s) 105, 330
DpnI GATC 2 cut(s) 216, 286
DpnII GATC 2 cut(s) 214, 284
DraI TTTAAA 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 313
EciI GGCGGA 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 141
FaeI CATG 1 cut(s) 277
FaiI YATR 5 cut(s) 50, 115, 275, 404, 406
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 53
FspBI CTAG 1 cut(s) 207
GluI GCNGC 1 cut(s) 53
HaeIII GGCC 2 cut(s) 147, 315
Hin1II CATG 1 cut(s) 277
HinfI GANTC 1 cut(s) 8
Hpy166II GTNNAC 3 cut(s) 223, 304, 345
Hpy8I GTNNAC 3 cut(s) 223, 304, 345
HpyAV CCTTC 2 cut(s) 46, 115
HpyCH4III ACNGT 3 cut(s) 88, 247, 349
HpyCH4IV ACGT 2 cut(s) 127, 388
HpyCH4V TGCA 1 cut(s) 52
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 335
HpyF3I CTNAG 2 cut(s) 105, 330
HpySE526I ACGT 2 cut(s) 127, 388
Hsp92II CATG 1 cut(s) 277
Kzo9I GATC 2 cut(s) 214, 284
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 4 cut(s) 57, 117, 266, 348
Lsp1109I GCAGC 1 cut(s) 64
MaeI CTAG 1 cut(s) 207
MaeII ACGT 2 cut(s) 127, 388
MaeIII GTNAC 2 cut(s) 76, 247
MalI GATC 2 cut(s) 216, 286
MboI GATC 2 cut(s) 214, 284
MboII GAAGA 1 cut(s) 190
MluCI AATT 3 cut(s) 156, 267, 380
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 5 cut(s) 79, 137, 150, 285, 377
MseI TTAA 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 174, 335
NdeII GATC 2 cut(s) 214, 284
NlaIII CATG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 277
PaeR7I CTCGAG 1 cut(s) 141
PciI ACATGT 1 cut(s) 273
PkrI GCNGC 1 cut(s) 54
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PscI ACATGT 1 cut(s) 273
PspPI GGNCC 1 cut(s) 137
PspXI VCTCGAGB 1 cut(s) 141
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 1 cut(s) 53
Sau3AI GATC 2 cut(s) 214, 284
Sau96I GGNCC 1 cut(s) 137
ScaI AGTACT 1 cut(s) 103
SchI GAGTC 1 cut(s) 2
SetI ASST 8 cut(s) 38, 57, 71, 96, 130, 208, 369, 391
Sfr274I CTCGAG 1 cut(s) 141
SinI GGWCC 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 3 cut(s) 156, 267, 380
SsiI CCGC 2 cut(s) 58, 186
SspMI CTAG 1 cut(s) 207
TaaI ACNGT 3 cut(s) 88, 247, 349
TaiI ACGT 2 cut(s) 130, 391
TaqI TCGA 4 cut(s) 11, 142, 242, 393
TasI AATT 3 cut(s) 156, 267, 380
TatI WGTACW 1 cut(s) 101
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 91, 378
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 195, 245
TspRI CASTG 2 cut(s) 91, 378
VpaK11BI GGWCC 1 cut(s) 137
XapI RAATTY 1 cut(s) 380
XceI RCATGY 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 141
XspI CTAG 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.