Rroxscaffold_5G00383190

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003500
Physical Location & Seq
Reverse (-)
62869366 .. 62870231
866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383190.1

Sequence Viewer

Length: 360 bp
ATGCAGCTCCGCCCAATGACCTCTGGTGTCACTCTCACTGTAAAGGTTGTTAGTACTAAGATAGTATGCCCGAAGGGACGTGCTGGTGGTCCTCGAGGCCAAATGCAAATTGCTGATTGCTTGGTTGGCGATGAAACGGCAATGATAATCTTCACAGCTAGAAACGATCAAGTGGACTTGATGAAAGAGGGTTCGACTGTAACCCTGCGAAATGCCAAAATTGACATGTTTAAAGGATCAATGAGGCTTGCTGTGGACAAAAGTGGCCGTATTGAAGTTGCTGAGCCTGCCAAGTTTACCGTGATGGAAACTAACAACCTCTCACTCATTGAATTTGAACGTGTCGATGTCGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.02

Weight (kDa)

8.68

Isoelectric Point (pI)

12.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 3 - 93 2.4e-40 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 93
AciI CCGC 1 cut(s) 10
AclWI GGATC 1 cut(s) 244
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 1 cut(s) 332
AfaI GTAC 1 cut(s) 55
AflIII ACRYGT 2 cut(s) 225, 340
AgsI TTSAA 3 cut(s) 275, 332, 338
AjiI CACGTC 1 cut(s) 80
AluBI AGCT 2 cut(s) 7, 158
AluI AGCT 2 cut(s) 7, 158
AlwI GGATC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 2 cut(s) 97, 265
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 1 cut(s) 89
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 298
BceAI ACGGC 2 cut(s) 153, 252
BfaI CTAG 1 cut(s) 159
BisI GCNGC 1 cut(s) 5
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 55
Bme18I GGWCC 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 93
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 89
Bpu1102I GCTNAGC 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 273
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 2 cut(s) 99, 267
BsiHKCI CYCGRG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 90
BsnI GGCC 2 cut(s) 99, 267
BsoBI CYCGRG 1 cut(s) 93
Bsp143I GATC 2 cut(s) 166, 236
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 1 cut(s) 10
BspANI GGCC 2 cut(s) 99, 267
BspCNI CTCAG 1 cut(s) 274
BspPI GGATC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 147
BssMI GATC 2 cut(s) 166, 236
Bst4CI ACNGT 3 cut(s) 40, 199, 301
BstC8I GCNNGC 2 cut(s) 249, 288
BstDEI CTNAG 2 cut(s) 57, 282
BstKTI GATC 2 cut(s) 169, 239
BstMBI GATC 2 cut(s) 166, 236
BstMWI GCNNNNNNNGC 2 cut(s) 126, 287
BstNSI RCATGY 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 16
BsuRI GGCC 2 cut(s) 99, 267
BtgZI GCGATG 1 cut(s) 144
BtrI CACGTC 1 cut(s) 80
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 249, 288
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 1 cut(s) 54
CviAII CATG 1 cut(s) 226
CviJI RGCY 6 cut(s) 7, 99, 158, 247, 267, 286
CviKI_1 RGCY 6 cut(s) 7, 99, 158, 247, 267, 286
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 2 cut(s) 57, 282
DpnI GATC 2 cut(s) 168, 238
DpnII GATC 2 cut(s) 166, 236
DraI TTTAAA 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 89
Eco88I CYCGRG 1 cut(s) 93
FaeI CATG 1 cut(s) 229
FaiI YATR 4 cut(s) 67, 227, 356, 358
FaqI GGGAC 1 cut(s) 90
FatI CATG 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 5
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 1 cut(s) 159
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 2 cut(s) 99, 267
Hin1II CATG 1 cut(s) 229
Hpy166II GTNNAC 3 cut(s) 175, 256, 297
Hpy8I GTNNAC 3 cut(s) 175, 256, 297
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 3 cut(s) 40, 199, 301
HpyCH4IV ACGT 2 cut(s) 79, 340
HpyCH4V TGCA 2 cut(s) 4, 106
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 287
HpyF3I CTNAG 2 cut(s) 57, 282
HpySE526I ACGT 2 cut(s) 79, 340
Hsp92II CATG 1 cut(s) 229
Kzo9I GATC 2 cut(s) 166, 236
LmnI GCTCC 1 cut(s) 12
LpnPI CCDG 4 cut(s) 9, 69, 218, 300
Lsp1109I GCAGC 1 cut(s) 16
MaeI CTAG 1 cut(s) 159
MaeII ACGT 2 cut(s) 79, 340
MaeIII GTNAC 2 cut(s) 28, 199
MalI GATC 2 cut(s) 168, 238
MboI GATC 2 cut(s) 166, 236
MboII GAAGA 1 cut(s) 142
MluCI AATT 3 cut(s) 108, 219, 332
MnlI CCTC 6 cut(s) 31, 89, 102, 181, 237, 329
MseI TTAA 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 126, 287
NdeII GATC 2 cut(s) 166, 236
NlaIII CATG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 28
NspI RCATGY 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 93
PciI ACATGT 1 cut(s) 225
PkrI GCNGC 1 cut(s) 6
PscI ACATGT 1 cut(s) 225
PspPI GGNCC 1 cut(s) 89
PspXI VCTCGAGB 1 cut(s) 93
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 2 cut(s) 166, 236
Sau96I GGNCC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 55
SetI ASST 7 cut(s) 9, 23, 48, 82, 160, 321, 343
Sfr274I CTCGAG 1 cut(s) 93
SinI GGWCC 1 cut(s) 89
SlaI CTCGAG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
Sse9I AATT 3 cut(s) 108, 219, 332
SsiI CCGC 1 cut(s) 10
SspMI CTAG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 40, 199, 301
TaiI ACGT 2 cut(s) 82, 343
TaqI TCGA 3 cut(s) 94, 194, 345
TasI AATT 3 cut(s) 108, 219, 332
TatI WGTACW 1 cut(s) 53
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 147, 197
TspRI CASTG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 1 cut(s) 332
XceI RCATGY 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 93
XspI CTAG 1 cut(s) 159
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.