Rh4CG420700

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003500
Physical Location & Seq
Reverse (-)
66477750 .. 66480261
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG420700.1

Sequence Viewer

Length: 498 bp
ATGTATCTACTGATAGTTTTTAAATTTATTACTCTGTGTCCCATCCATCTCCCCAGTTGTTTGAGCCAGACTCATAAGGTCTTTTCTCAAATGGCAGACTCGAAACAAGCACCGAGCAAACCAACCTTCACAAAGGTTATGCAGCTCCGCCCAATGACCTCTGGTGTCACTCTCACTGTAAAGGTTGTTAATACTAAGATAGTATGTCCGAAGGGACGTGCTGGTGGGCCTCGAGGCCAAATGCGAATTGCTGATTGCTTGGTTGGCGATGAAACGGCAATGATAATCTTCACGGCTAGAAACGATCAAGTGGACTTGATGAAAGAGGGTTCGACTGTAACCCTGCGAAATGCCAAAATTGACATGTTTAAAGGATCAATGAGGCTTGCTGTGGACAAAAGTGGCCGTATTGAAGTTGCTGAGCCTGCCAATTTTACCGTGATGGAAAATAACAACCTCTCACTCATTGAATTTGAACGTGTCGATGTCGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.28

Weight (kDa)

9.33

Isoelectric Point (pI)

26.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 49 - 139 4.2e-40 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 231
AciI CCGC 1 cut(s) 148
AclWI GGATC 1 cut(s) 382
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 23, 470
AflIII ACRYGT 2 cut(s) 363, 478
AgsI TTSAA 3 cut(s) 413, 470, 476
AjiI CACGTC 1 cut(s) 218
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
AlwI GGATC 1 cut(s) 382
Ama87I CYCGRG 1 cut(s) 231
AoxI GGCC 3 cut(s) 227, 235, 403
ApeKI GCWGC 1 cut(s) 142
ApoI RAATTY 2 cut(s) 23, 470
AspS9I GGNCC 1 cut(s) 227
AvaI CYCGRG 1 cut(s) 231
BbvI GCAGC 1 cut(s) 154
BccI CCATC 3 cut(s) 50, 54, 436
BceAI ACGGC 3 cut(s) 291, 309, 390
BfaI CTAG 1 cut(s) 297
BisI GCNGC 1 cut(s) 143
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 144
BmeT110I CYCGRG 1 cut(s) 231
BmgBI CACGTC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 48
BmuI ACTGGG 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 55, 87
Bpu1102I GCTNAGC 1 cut(s) 420
Bse1I ACTGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 285
BseGI GGATG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 154
BshFI GGCC 3 cut(s) 229, 237, 405
BsiHKCI CYCGRG 1 cut(s) 231
BslFI GGGAC 2 cut(s) 24, 228
BsmFI GGGAC 2 cut(s) 24, 228
BsnI GGCC 3 cut(s) 229, 237, 405
BsoBI CYCGRG 1 cut(s) 231
Bsp143I GATC 2 cut(s) 304, 374
Bsp1720I GCTNAGC 1 cut(s) 420
BspACI CCGC 1 cut(s) 148
BspANI GGCC 3 cut(s) 229, 237, 405
BspCNI CTCAG 1 cut(s) 412
BspPI GGATC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 1 cut(s) 54
BssMI GATC 2 cut(s) 304, 374
Bst4CI ACNGT 3 cut(s) 178, 337, 439
BstC8I GCNNGC 2 cut(s) 387, 426
BstDEI CTNAG 2 cut(s) 195, 420
BstF5I GGATG 1 cut(s) 42
BstKTI GATC 2 cut(s) 307, 377
BstMBI GATC 2 cut(s) 304, 374
BstMWI GCNNNNNNNGC 2 cut(s) 264, 425
BstNSI RCATGY 1 cut(s) 367
BstV1I GCAGC 1 cut(s) 154
BsuRI GGCC 3 cut(s) 229, 237, 405
BtgZI GCGATG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 218
BtsCI GGATG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 387, 426
Cfr13I GGNCC 1 cut(s) 227
CviAII CATG 1 cut(s) 364
CviJI RGCY 8 cut(s) 66, 145, 229, 237, 296, 385, 405, 424
CviKI_1 RGCY 8 cut(s) 66, 145, 229, 237, 296, 385, 405, 424
DdeI CTNAG 2 cut(s) 195, 420
DpnI GATC 2 cut(s) 306, 376
DpnII GATC 2 cut(s) 304, 374
DraI TTTAAA 2 cut(s) 22, 370
EaeI YGGCCR 1 cut(s) 403
EciI GGCGGA 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 231
FaeI CATG 1 cut(s) 367
FaiI YATR 6 cut(s) 75, 140, 205, 365, 494, 496
FaqI GGGAC 2 cut(s) 24, 228
FatI CATG 1 cut(s) 363
Fnu4HI GCNGC 1 cut(s) 143
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 143
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 143
HaeIII GGCC 3 cut(s) 229, 237, 405
Hin1II CATG 1 cut(s) 367
HinfI GANTC 2 cut(s) 70, 98
Hpy166II GTNNAC 2 cut(s) 313, 394
Hpy188I TCNGA 1 cut(s) 210
Hpy8I GTNNAC 2 cut(s) 313, 394
HpyAV CCTTC 2 cut(s) 136, 205
HpyCH4III ACNGT 3 cut(s) 178, 337, 439
HpyCH4IV ACGT 2 cut(s) 217, 478
HpyCH4V TGCA 1 cut(s) 142
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 425
HpyF3I CTNAG 2 cut(s) 195, 420
HpySE526I ACGT 2 cut(s) 217, 478
Hsp92II CATG 1 cut(s) 367
Kzo9I GATC 2 cut(s) 304, 374
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 6 cut(s) 67, 80, 147, 207, 356, 438
Lsp1109I GCAGC 1 cut(s) 154
MaeI CTAG 1 cut(s) 297
MaeII ACGT 2 cut(s) 217, 478
MaeIII GTNAC 2 cut(s) 166, 337
MalI GATC 2 cut(s) 306, 376
MboI GATC 2 cut(s) 304, 374
MboII GAAGA 1 cut(s) 280
MluCI AATT 5 cut(s) 23, 246, 357, 430, 470
MlyI GAGTC 2 cut(s) 64, 92
MnlI CCTC 6 cut(s) 169, 227, 240, 319, 375, 467
MseI TTAA 3 cut(s) 21, 189, 369
MwoI GCNNNNNNNGC 2 cut(s) 264, 425
NdeII GATC 2 cut(s) 304, 374
NlaIII CATG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 231
PciI ACATGT 1 cut(s) 363
PkrI GCNGC 1 cut(s) 144
PleI GAGTC 2 cut(s) 64, 92
PpsI GAGTC 2 cut(s) 64, 92
PscI ACATGT 1 cut(s) 363
PspPI GGNCC 1 cut(s) 227
PspXI VCTCGAGB 1 cut(s) 231
SaqAI TTAA 3 cut(s) 21, 189, 369
SatI GCNGC 1 cut(s) 143
Sau3AI GATC 2 cut(s) 304, 374
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 2 cut(s) 64, 92
SetI ASST 9 cut(s) 81, 128, 138, 147, 161, 186, 220, 459, 481
Sfr274I CTCGAG 1 cut(s) 231
SlaI CTCGAG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
Sse9I AATT 5 cut(s) 23, 246, 357, 430, 470
SsiI CCGC 1 cut(s) 148
SspMI CTAG 1 cut(s) 297
TaaI ACNGT 3 cut(s) 178, 337, 439
TaiI ACGT 2 cut(s) 220, 481
TaqI TCGA 4 cut(s) 101, 232, 332, 483
TasI AATT 5 cut(s) 23, 246, 357, 430, 470
Tru1I TTAA 3 cut(s) 21, 189, 369
Tru9I TTAA 3 cut(s) 21, 189, 369
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 285, 335
TspRI CASTG 1 cut(s) 181
XapI RAATTY 2 cut(s) 23, 470
XceI RCATGY 1 cut(s) 367
XhoI CTCGAG 1 cut(s) 231
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.