RLG00000005882

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003500
Physical Location & Seq
Forward (+)
2530405 .. 2533277
2873 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005882

Sequence Viewer

Length: 555 bp
ATGCTAAAGCCAGTTAAATATGCAATTCTCAGACATAGGTGGACGACTGGACGTCTACGAAGAACTCCTCTCTCTCTCTCTCCCTCTCCTCCCCCATCTCCCCAGTTGTGTTTGAGCCAAGCTCGTAAGGTCTTTTCTCAAATGGCAGACTCGAAACAAGCACTGAGGAAACCAACCTTCACAAAGGTCGGGCAGCTCCGTCCAAGCACCTCTGGTCACACTCTCACTGTAAAGGTTGTTAGTACTAAGATGGTATTGCAGAAGGGACGTGCTGATGGCCCTCAAGTACGCCAAATGCGAATTGCTGAATGCTTGGTTGGCGATGAAACGGGAATGATAATCTTCACAGCTAGAAACGATCAAGTGGACTTGATGAAAGAGGGTTCGACTGTAACCCTGCGGAATGCCAAAATTGACATGTTCAAAGGATCAATGAGGCTTGCTGTGGACAAATGGGGCCGAGTTGAAGTTGCTGAGCCTGCCAGTTTTACTGTGATGGAAGATAACAACCTTTCACTCATTGAATATGAACTTGTCAATGTTGTTGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.61

Weight (kDa)

9.95

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 66 - 157 1e-45 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 55
AccI GTMKAC 1 cut(s) 55
AciI CCGC 1 cut(s) 400
AclWI GGATC 1 cut(s) 436
AcyI GRCGYC 1 cut(s) 52
AfaI GTAC 2 cut(s) 244, 288
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 4 cut(s) 424, 467, 524, 548
AhdI GACNNNNNGTC 1 cut(s) 51
AjiI CACGTC 1 cut(s) 269
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AluBI AGCT 3 cut(s) 122, 196, 350
AluI AGCT 3 cut(s) 122, 196, 350
AlwI GGATC 1 cut(s) 436
AoxI GGCC 2 cut(s) 277, 457
ApeKI GCWGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 278, 457
BbvI GCAGC 1 cut(s) 205
BccI CCATC 4 cut(s) 103, 244, 269, 490
BfaI CTAG 1 cut(s) 351
BisI GCNGC 1 cut(s) 194
BlpI GCTNAGC 1 cut(s) 474
BlsI GCNGC 1 cut(s) 195
BmcAI AGTACT 1 cut(s) 244
BmeRI GACNNNNNGTC 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 2 cut(s) 278, 457
BmiI GGNNCC 1 cut(s) 458
BmrI ACTGGG 1 cut(s) 97
BmuI ACTGGG 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 106, 138
Bpu1102I GCTNAGC 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 52
Bse1I ACTGG 4 cut(s) 11, 52, 103, 483
BseMII CTCAG 3 cut(s) 43, 155, 465
BseNI ACTGG 4 cut(s) 11, 52, 103, 483
BseRI GAGGAG 2 cut(s) 57, 78
BseXI GCAGC 1 cut(s) 205
BshFI GGCC 2 cut(s) 279, 459
BslFI GGGAC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 2 cut(s) 314, 409
BsnI GGCC 2 cut(s) 279, 459
Bsp143I GATC 2 cut(s) 358, 428
Bsp1720I GCTNAGC 1 cut(s) 474
BspACI CCGC 1 cut(s) 400
BspANI GGCC 2 cut(s) 279, 459
BspCNI CTCAG 3 cut(s) 42, 156, 466
BspLI GGNNCC 1 cut(s) 458
BspPI GGATC 1 cut(s) 436
BsrI ACTGG 4 cut(s) 11, 52, 103, 483
BssMI GATC 2 cut(s) 358, 428
BssNI GRCGYC 1 cut(s) 52
Bst4CI ACNGT 3 cut(s) 229, 391, 493
Bst6I CTCTTC 1 cut(s) 543
BstACI GRCGYC 1 cut(s) 52
BstC8I GCNNGC 2 cut(s) 441, 480
BstDEI CTNAG 4 cut(s) 29, 164, 246, 474
BstKTI GATC 2 cut(s) 361, 431
BstMBI GATC 2 cut(s) 358, 428
BstMWI GCNNNNNNNGC 2 cut(s) 318, 479
BstNSI RCATGY 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 205
BsuRI GGCC 2 cut(s) 279, 459
BtgZI GCGATG 1 cut(s) 336
BtrI CACGTC 1 cut(s) 269
BtsIMutI CAGTG 2 cut(s) 161, 225
Cac8I GCNNGC 2 cut(s) 441, 480
Cfr13I GGNCC 2 cut(s) 278, 457
Csp6I GTAC 2 cut(s) 243, 287
CviAII CATG 1 cut(s) 418
CviJI RGCY 9 cut(s) 10, 117, 122, 196, 279, 350, 439, 459, 478
CviKI_1 RGCY 9 cut(s) 10, 117, 122, 196, 279, 350, 439, 459, 478
CviQI GTAC 2 cut(s) 243, 287
DdeI CTNAG 4 cut(s) 29, 164, 246, 474
DpnI GATC 2 cut(s) 360, 430
DpnII GATC 2 cut(s) 358, 428
DriI GACNNNNNGTC 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 543
Eam1105I GACNNNNNGTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 543
FaeI CATG 1 cut(s) 421
FaiI YATR 4 cut(s) 21, 36, 419, 528
FaqI GGGAC 1 cut(s) 279
FatI CATG 1 cut(s) 417
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 194
Fsp4HI GCNGC 1 cut(s) 194
FspBI CTAG 1 cut(s) 351
GluI GCNGC 1 cut(s) 194
HaeIII GGCC 2 cut(s) 279, 459
Hin1I GRCGYC 1 cut(s) 52
Hin1II CATG 1 cut(s) 421
HinfI GANTC 1 cut(s) 149
Hpy166II GTNNAC 4 cut(s) 42, 56, 367, 448
Hpy188I TCNGA 1 cut(s) 32
Hpy8I GTNNAC 4 cut(s) 42, 56, 367, 448
HpyAV CCTTC 2 cut(s) 187, 256
HpyCH4III ACNGT 3 cut(s) 229, 391, 493
HpyCH4IV ACGT 2 cut(s) 52, 268
HpyCH4V TGCA 2 cut(s) 23, 259
HpyF10VI GCNNNNNNNGC 2 cut(s) 318, 479
HpyF3I CTNAG 4 cut(s) 29, 164, 246, 474
HpySE526I ACGT 2 cut(s) 52, 268
Hsp92I GRCGYC 1 cut(s) 52
Hsp92II CATG 1 cut(s) 421
Kzo9I GATC 2 cut(s) 358, 428
LmnI GCTCC 1 cut(s) 201
LpnPI CCDG 7 cut(s) 24, 33, 116, 198, 410, 492, 496
Lsp1109I GCAGC 1 cut(s) 205
MaeI CTAG 1 cut(s) 351
MaeII ACGT 2 cut(s) 52, 268
MaeIII GTNAC 2 cut(s) 215, 391
MalI GATC 2 cut(s) 360, 430
MboI GATC 2 cut(s) 358, 428
MboII GAAGA 3 cut(s) 72, 334, 512
MluCI AATT 3 cut(s) 24, 300, 411
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 8 cut(s) 78, 94, 99, 159, 220, 291, 373, 429
MseI TTAA 1 cut(s) 15
Mva1269I GAATGC 2 cut(s) 314, 409
MwoI GCNNNNNNNGC 2 cut(s) 318, 479
NdeII GATC 2 cut(s) 358, 428
NlaIII CATG 1 cut(s) 421
NlaIV GGNNCC 1 cut(s) 458
NmeAIII GCCGAG 1 cut(s) 485
NmuCI GTSAC 1 cut(s) 215
NspI RCATGY 1 cut(s) 421
PciI ACATGT 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 295
PctI GAATGC 2 cut(s) 314, 409
PkrI GCNGC 1 cut(s) 195
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PscI ACATGT 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 458
PspPI GGNCC 2 cut(s) 278, 457
RsaI GTAC 2 cut(s) 244, 288
RsaNI GTAC 2 cut(s) 243, 287
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 1 cut(s) 194
Sau3AI GATC 2 cut(s) 358, 428
Sau96I GGNCC 2 cut(s) 278, 457
ScaI AGTACT 1 cut(s) 244
SchI GAGTC 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 3 cut(s) 24, 300, 411
SsiI CCGC 1 cut(s) 400
SspMI CTAG 1 cut(s) 351
TaaI ACNGT 3 cut(s) 229, 391, 493
TaiI ACGT 2 cut(s) 55, 271
TaqI TCGA 2 cut(s) 152, 386
TasI AATT 3 cut(s) 24, 300, 411
TatI WGTACW 1 cut(s) 242
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 168, 232
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 3 cut(s) 339, 389, 543
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 2 cut(s) 168, 232
XceI RCATGY 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 55
XspI CTAG 1 cut(s) 351
ZraI GACGTC 1 cut(s) 53
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.