FvH4_4g33300

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003500
Physical Location & Seq
Forward (+)
31574182 .. 31575019
838 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33300.t1

Sequence Viewer

Length: 384 bp
ATGGCAGACTCGAAACAAGCACCAGGCAAACCAATCTTCACAAAGGTTATGCAGCTCCGCCCAACGACCTCTGGTGTTACTCTGACCGTAAAGGTTGTTAGTACTAAGATGATAGTGCCGAAGGGACGTGCTGGTGGCCCTCGAAGCCAAATGCAAGTTGTTGAATGCTTGGTTGGCGATGAAACGGCAATGATAATCTTCAGGGCTAGAAACGAACAAGGTGAATCAGAGATCATAACTTTGTCCGACATGTTTAAAGGATCAATGAGGCTTGCTGTGGACAAAAGTGGCCGTGTTGAAGTTTCTGAGCCTGCTAGTTTTACTGTGATGGAGGATAACAACCTCTCGCTCATTGAATTTGAACGTCTCGATTTCGCTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

13.94

Weight (kDa)

6.31

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 101 7.8e-23 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 58
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 184
AfaI GTAC 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 4 cut(s) 164, 299, 356, 362
AjiI CACGTC 1 cut(s) 128
AjnI CCWGG 1 cut(s) 22
AjuI GAANNNNNNNTTGG 2 cut(s) 156, 188
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 1 cut(s) 268
AoxI GGCC 2 cut(s) 136, 289
ApeKI GCWGC 1 cut(s) 52
ApoI RAATTY 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 233
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 322
BceAI ACGGC 2 cut(s) 201, 276
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 207, 315
BisI GCNGC 1 cut(s) 53
BlsI GCNGC 1 cut(s) 54
BmcAI AGTACT 1 cut(s) 103
Bme1390I CCNGG 1 cut(s) 24
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 24
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 24
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 297
BseXI GCAGC 1 cut(s) 64
BshFI GGCC 2 cut(s) 138, 291
BslFI GGGAC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 2 cut(s) 138, 291
Bsp143I GATC 2 cut(s) 231, 260
BspACI CCGC 1 cut(s) 58
BspANI GGCC 2 cut(s) 138, 291
BspCNI CTCAG 1 cut(s) 298
BspPI GGATC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 195
BssMI GATC 2 cut(s) 231, 260
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 88, 325
BstC8I GCNNGC 2 cut(s) 273, 312
BstDEI CTNAG 2 cut(s) 105, 306
BstKTI GATC 2 cut(s) 234, 263
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 2 cut(s) 231, 260
BstMWI GCNNNNNNNGC 2 cut(s) 144, 174
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 64
BsuRI GGCC 2 cut(s) 138, 291
BtgZI GCGATG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 273, 312
Cfr13I GGNCC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 102
CviAII CATG 1 cut(s) 250
CviJI RGCY 7 cut(s) 55, 138, 147, 206, 271, 291, 310
CviKI_1 RGCY 7 cut(s) 55, 138, 147, 206, 271, 291, 310
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 2 cut(s) 105, 306
DpnI GATC 2 cut(s) 233, 262
DpnII GATC 2 cut(s) 231, 260
DraI TTTAAA 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 289
EciI GGCGGA 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 22
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 1 cut(s) 253
FaiI YATR 4 cut(s) 50, 236, 251, 380
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 249
Fnu4HI GCNGC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 53
FspBI CTAG 2 cut(s) 207, 315
GluI GCNGC 1 cut(s) 53
HaeIII GGCC 2 cut(s) 138, 291
Hin1II CATG 1 cut(s) 253
HinfI GANTC 2 cut(s) 8, 224
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 4 cut(s) 84, 229, 247, 307
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 2 cut(s) 88, 325
HpyCH4IV ACGT 2 cut(s) 127, 364
HpyCH4V TGCA 2 cut(s) 52, 154
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 174
HpyF3I CTNAG 2 cut(s) 105, 306
HpySE526I ACGT 2 cut(s) 127, 364
Hsp92II CATG 1 cut(s) 253
Kzo9I GATC 2 cut(s) 231, 260
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 6 cut(s) 9, 36, 57, 117, 187, 324
Lsp1109I GCAGC 1 cut(s) 64
MaeI CTAG 2 cut(s) 207, 315
MaeII ACGT 2 cut(s) 127, 364
MaeIII GTNAC 1 cut(s) 76
MalI GATC 2 cut(s) 233, 262
MboI GATC 2 cut(s) 231, 260
MboII GAAGA 2 cut(s) 28, 190
MluCI AATT 1 cut(s) 356
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 5 cut(s) 79, 150, 261, 325, 353
MseI TTAA 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 144, 174
NdeII GATC 2 cut(s) 231, 260
NlaIII CATG 1 cut(s) 253
NspI RCATGY 1 cut(s) 253
PciI ACATGT 1 cut(s) 249
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 54
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PscI ACATGT 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspPI GGNCC 1 cut(s) 137
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 53
Sau3AI GATC 2 cut(s) 231, 260
Sau96I GGNCC 1 cut(s) 137
ScaI AGTACT 1 cut(s) 103
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 24
SetI ASST 8 cut(s) 48, 57, 71, 96, 130, 223, 345, 367
Sse9I AATT 1 cut(s) 356
SsiI CCGC 1 cut(s) 58
SspMI CTAG 2 cut(s) 207, 315
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 88, 325
TaiI ACGT 2 cut(s) 130, 367
TaqI TCGA 3 cut(s) 11, 142, 369
TasI AATT 1 cut(s) 356
TatI WGTACW 1 cut(s) 101
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseI GCWGC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 195
XapI RAATTY 1 cut(s) 356
XceI RCATGY 1 cut(s) 253
XspI CTAG 2 cut(s) 207, 315
ZrmI AGTACT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.