Rh4CG433500

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003500
Physical Location & Seq
Reverse (-)
67286380 .. 67290527
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG433500.1

Sequence Viewer

Length: 567 bp
ATGGACATATGGAACTCAGCAACGATAGCTAAACCCTTTTTCACTCAGCAGTGCGTGCTCGACGACTTTGGGTTTTTCATAGTCTACGAAGATCTCTCTCTCTCTCTCTCCCCCCAGTTGTGTTTGAGCCAAGCTCGTAAGGTCTTTTCTCAAATGGCAGACTCGAAACAAGCACTGAGGAAACCAACCTTCACAAAGGTCGGGCAGCTCCGTCCAAGCACCTCTGGTCACACTCTCACTGTAAAGGTTGTTAGTACTAAGATGGTATTGCAGAAGGGACGTGCTGATGGCCCTCAAGTACGCCAAATGCGAATTGCTGAATGCTTGGTTGGTGATGAAACGGGAATGATAATCTTCACAGCTCGAAACGATCAAGTGGACTTGATGAAAGAGGGTTCGACTGTAACCCTGCGGAATGCCAAAATTGACATGTTCAAAGGATCAATGAGGCTTGCTGTGGACAAATGGGGCCGAGTTGAAGTTGCAGAGCCTGCCAGTTTTACCGTGATGGAAGATAACAACCTCTCACTCATTGAATATGAACTTGTCAATGTTGTTGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.06

Weight (kDa)

5.94

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 70 - 161 1.1e-45 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 84
AciI CCGC 1 cut(s) 412
AclWI GGATC 1 cut(s) 448
AfaI GTAC 2 cut(s) 256, 300
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 4 cut(s) 436, 479, 536, 560
AjiI CACGTC 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AluBI AGCT 4 cut(s) 29, 134, 208, 362
AluI AGCT 4 cut(s) 29, 134, 208, 362
Alw21I GWGCWC 1 cut(s) 60
AlwI GGATC 1 cut(s) 448
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 2 cut(s) 289, 469
ApeKI GCWGC 1 cut(s) 205
AspS9I GGNCC 2 cut(s) 290, 469
AsuHPI GGTGA 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 217
BccI CCATC 3 cut(s) 256, 281, 502
BglII AGATCT 1 cut(s) 91
BisI GCNGC 1 cut(s) 206
BlsI GCNGC 1 cut(s) 207
BmcAI AGTACT 1 cut(s) 256
BmgBI CACGTC 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 290, 469
BmiI GGNNCC 1 cut(s) 470
BmrI ACTGGG 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 109
BplI GAGNNNNNCTC 2 cut(s) 118, 150
BpuEI CTTGAG 1 cut(s) 279
Bse1I ACTGG 2 cut(s) 115, 495
BseMII CTCAG 3 cut(s) 30, 59, 167
BseNI ACTGG 2 cut(s) 115, 495
BseXI GCAGC 1 cut(s) 217
BshFI GGCC 2 cut(s) 291, 471
BsiHKAI GWGCWC 1 cut(s) 60
BslFI GGGAC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 291
BsmI GAATGC 2 cut(s) 326, 421
BsnI GGCC 2 cut(s) 291, 471
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 3 cut(s) 91, 370, 440
BspACI CCGC 1 cut(s) 412
BspANI GGCC 2 cut(s) 291, 471
BspCNI CTCAG 3 cut(s) 29, 58, 168
BspLI GGNNCC 1 cut(s) 470
BspPI GGATC 1 cut(s) 448
BsrI ACTGG 2 cut(s) 115, 495
BssMI GATC 3 cut(s) 91, 370, 440
Bst4CI ACNGT 3 cut(s) 241, 403, 505
Bst6I CTCTTC 1 cut(s) 555
BstAPI GCANNNNNTGC 2 cut(s) 55, 491
BstC8I GCNNGC 3 cut(s) 56, 453, 492
BstDEI CTNAG 4 cut(s) 16, 45, 176, 258
BstKTI GATC 3 cut(s) 94, 373, 443
BstMBI GATC 3 cut(s) 91, 370, 440
BstMWI GCNNNNNNNGC 3 cut(s) 26, 55, 491
BstNSI RCATGY 1 cut(s) 433
BstV1I GCAGC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuRI GGCC 2 cut(s) 291, 471
BtrI CACGTC 1 cut(s) 281
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 3 cut(s) 56, 173, 237
Cac8I GCNNGC 3 cut(s) 56, 453, 492
CaiI CAGNNNCTG 1 cut(s) 491
Cfr13I GGNCC 2 cut(s) 290, 469
Csp6I GTAC 2 cut(s) 255, 299
CviAII CATG 1 cut(s) 430
CviJI RGCY 9 cut(s) 29, 129, 134, 208, 291, 362, 451, 471, 490
CviKI_1 RGCY 9 cut(s) 29, 129, 134, 208, 291, 362, 451, 471, 490
CviQI GTAC 2 cut(s) 255, 299
DdeI CTNAG 4 cut(s) 16, 45, 176, 258
DpnI GATC 3 cut(s) 93, 372, 442
DpnII GATC 3 cut(s) 91, 370, 440
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
FaeI CATG 1 cut(s) 433
FaiI YATR 5 cut(s) 8, 10, 80, 431, 540
FaqI GGGAC 1 cut(s) 291
FatI CATG 1 cut(s) 429
FauNDI CATATG 1 cut(s) 8
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 206
GluI GCNGC 1 cut(s) 206
HaeIII GGCC 2 cut(s) 291, 471
Hin1II CATG 1 cut(s) 433
HinfI GANTC 1 cut(s) 161
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 85, 379, 460
Hpy8I GTNNAC 3 cut(s) 85, 379, 460
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 199, 268
HpyCH4III ACNGT 3 cut(s) 241, 403, 505
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 2 cut(s) 271, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 55, 491
HpyF3I CTNAG 4 cut(s) 16, 45, 176, 258
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 1 cut(s) 433
Kzo9I GATC 3 cut(s) 91, 370, 440
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 5 cut(s) 128, 210, 422, 504, 508
Lsp1109I GCAGC 1 cut(s) 217
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 2 cut(s) 227, 403
MalI GATC 3 cut(s) 93, 372, 442
MboI GATC 3 cut(s) 91, 370, 440
MboII GAAGA 3 cut(s) 101, 346, 524
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 2 cut(s) 312, 423
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 6 cut(s) 171, 232, 303, 385, 441, 533
Mva1269I GAATGC 2 cut(s) 326, 421
MwoI GCNNNNNNNGC 3 cut(s) 26, 55, 491
NdeI CATATG 1 cut(s) 8
NdeII GATC 3 cut(s) 91, 370, 440
NlaIII CATG 1 cut(s) 433
NlaIV GGNNCC 1 cut(s) 470
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 1 cut(s) 227
NspI RCATGY 1 cut(s) 433
PciI ACATGT 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 307
PctI GAATGC 2 cut(s) 326, 421
PkrI GCNGC 1 cut(s) 207
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PscI ACATGT 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 470
PspPI GGNCC 2 cut(s) 290, 469
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 2 cut(s) 256, 300
RsaNI GTAC 2 cut(s) 255, 299
SatI GCNGC 1 cut(s) 206
Sau3AI GATC 3 cut(s) 91, 370, 440
Sau96I GGNCC 2 cut(s) 290, 469
ScaI AGTACT 1 cut(s) 256
SchI GAGTC 1 cut(s) 155
SduI GDGCHC 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
Sse9I AATT 2 cut(s) 312, 423
SsiI CCGC 1 cut(s) 412
TaaI ACNGT 3 cut(s) 241, 403, 505
TaiI ACGT 1 cut(s) 283
TaqI TCGA 4 cut(s) 60, 164, 364, 398
TasI AATT 2 cut(s) 312, 423
TatI WGTACW 1 cut(s) 254
TscAI CASTG 3 cut(s) 56, 180, 244
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 4 cut(s) 67, 351, 401, 555
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 3 cut(s) 56, 180, 244
XceI RCATGY 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.