AT1G23750

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003500
Physical Location & Seq
Forward (+)
8400056 .. 8401550
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23750.1

Sequence Viewer

Length: 414 bp
ATGGCAGATTCCACCAAAGCAGGGTTGAAGAAGCCAGCTTTCACTAAGGTTGATCAGCTGCGTCCTGGGACTAGCGGACACAATGTCAATGTGAAGATTGTCAGCACGAAAATGGTGTTACAGAAAGGTCGTGCTGATGGTCCTCAAGCTCGCCAGTTGCGGATTTCTGAATGCATTGTTGGTGACGAGACAGGAGTGATCGTCTTTACCGCAAGAAATGACCAAGTGGACTTAATGAAAGAGGGATCAACTGTAACTCTACGCAATGCGAAAATCGACATGTATAAAGGATCAATGAGGCTAGCTGTAGACAAGTGGGGTCGTGTTGAAGTCACAGAGCCTGCGAGCTTCAAAGTGAAAGAAGACACCAACATGTCCCTTATCGAGTATGAGCTCGTAAACGTCGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.13

Weight (kDa)

9.04

Isoelectric Point (pI)

27.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 20 - 111 4.5e-44 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AciI CCGC 3 cut(s) 75, 160, 210
AclWI GGATC 2 cut(s) 253, 298
AfiI CCNNNNNNNGG 1 cut(s) 21
AflIII ACRYGT 2 cut(s) 279, 372
AgsI TTSAA 4 cut(s) 28, 329, 352, 410
AhdI GACNNNNNGTC 1 cut(s) 83
AjnI CCWGG 1 cut(s) 64
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 6 cut(s) 38, 58, 149, 305, 348, 394
AluI AGCT 6 cut(s) 38, 58, 149, 305, 348, 394
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 2 cut(s) 253, 298
AlwNI CAGNNNCTG 1 cut(s) 341
ApeKI GCWGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 194
AsuNHI GCTAGC 1 cut(s) 301
AvaII GGWCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 45
BccI CCATC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 66
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 2 cut(s) 72, 302
BfmI CTRYAG 1 cut(s) 306
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 1 cut(s) 140
BmeRI GACNNNNNGTC 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 66
BmtI GCTAGC 1 cut(s) 305
BpiI GAAGAC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 65
BseLI CCNNNNNNNGG 1 cut(s) 21
BseMI GCAATG 1 cut(s) 271
BseNI ACTGG 1 cut(s) 154
BseXI GCAGC 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 396
BslFI GGGAC 2 cut(s) 82, 361
BslI CCNNNNNNNGG 1 cut(s) 21
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 82, 361
BsmI GAATGC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 4 cut(s) 52, 198, 245, 290
BspACI CCGC 3 cut(s) 75, 160, 210
BspOI GCTAGC 1 cut(s) 305
BspPI GGATC 2 cut(s) 253, 298
BsrDI GCAATG 1 cut(s) 271
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 4 cut(s) 52, 198, 245, 290
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 253
BstC8I GCNNGC 5 cut(s) 36, 151, 303, 342, 346
BstDEI CTNAG 1 cut(s) 45
BstKTI GATC 4 cut(s) 55, 201, 248, 293
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 4 cut(s) 52, 198, 245, 290
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 2 cut(s) 283, 376
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 369
Cac8I GCNNGC 5 cut(s) 36, 151, 303, 342, 346
CaiI CAGNNNCTG 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 140
CseI GACGC 1 cut(s) 50
CviAII CATG 2 cut(s) 280, 373
CviJI RGCY 9 cut(s) 34, 38, 58, 149, 301, 305, 340, 348, 394
CviKI_1 RGCY 9 cut(s) 34, 38, 58, 149, 301, 305, 340, 348, 394
DdeI CTNAG 1 cut(s) 45
DpnI GATC 4 cut(s) 54, 200, 247, 292
DpnII GATC 4 cut(s) 52, 198, 245, 290
DriI GACNNNNNGTC 1 cut(s) 83
Eam1105I GACNNNNNGTC 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 140
Eco53kI GAGCTC 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 396
FaeI CATG 2 cut(s) 283, 376
FaiI YATR 4 cut(s) 281, 285, 374, 390
FaqI GGGAC 2 cut(s) 82, 361
FatI CATG 2 cut(s) 279, 372
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 1 cut(s) 59
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 2 cut(s) 72, 302
GluI GCNGC 1 cut(s) 59
HgaI GACGC 1 cut(s) 50
Hin1II CATG 2 cut(s) 283, 376
HinfI GANTC 1 cut(s) 8
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 3 cut(s) 229, 310, 400
Hpy188I TCNGA 1 cut(s) 169
Hpy8I GTNNAC 3 cut(s) 229, 310, 400
Hpy99I CGWCG 1 cut(s) 407
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 1 cut(s) 174
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 2 cut(s) 283, 376
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 198, 245, 290
LpnPI CCDG 7 cut(s) 6, 48, 51, 78, 167, 177, 354
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 2 cut(s) 72, 302
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 4 cut(s) 117, 182, 253, 331
MalI GATC 4 cut(s) 54, 200, 247, 292
MboI GATC 4 cut(s) 52, 198, 245, 290
MboII GAAGA 3 cut(s) 40, 106, 374
MhlI GDGCHC 1 cut(s) 396
MnlI CCTC 3 cut(s) 153, 235, 291
Mph1103I ATGCAT 1 cut(s) 176
MseI TTAA 1 cut(s) 233
MslI CAYNNNNRTG 2 cut(s) 110, 371
MspA1I CMGCKG 1 cut(s) 58
MspR9I CCNGG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 66
NdeII GATC 4 cut(s) 52, 198, 245, 290
NheI GCTAGC 1 cut(s) 301
NlaIII CATG 2 cut(s) 283, 376
NmuCI GTSAC 2 cut(s) 182, 331
NsiI ATGCAT 1 cut(s) 176
NspI RCATGY 2 cut(s) 283, 376
PciI ACATGT 2 cut(s) 279, 372
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 1 cut(s) 60
PscI ACATGT 2 cut(s) 279, 372
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspPI GGNCC 1 cut(s) 140
PstNI CAGNNNCTG 1 cut(s) 341
PvuII CAGCTG 1 cut(s) 58
RseI CAYNNNNRTG 2 cut(s) 110, 371
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 4 cut(s) 52, 198, 245, 290
Sau96I GGNCC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 1 cut(s) 396
SetI ASST 9 cut(s) 40, 51, 60, 130, 151, 307, 350, 396, 405
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 110, 371
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
SsiI CCGC 3 cut(s) 75, 160, 210
SspMI CTAG 2 cut(s) 72, 302
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 1 cut(s) 405
TaqI TCGA 2 cut(s) 276, 384
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TseFI GTSAC 2 cut(s) 182, 331
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 2 cut(s) 182, 331
TspDTI ATGAA 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 140
XceI RCATGY 2 cut(s) 283, 376
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 2 cut(s) 72, 302
Zsp2I ATGCAT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.