Rh4AG392200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018178
Physical Location & Seq
Reverse (-)
69849971 .. 69852778
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG392200.1

Sequence Viewer

Length: 1122 bp
ATGGCGAAGCAGCCACCCATAGGGCCAAAGCGTCCGGACCAGGCCCAGAACAAGAAGGGCCAGCCTTCCAATTGGGTCTCCGGGACAGATCCCTCAGACGCGTCGCGAAGATGGATGGGAGTGGGCCCATTCGATGTTTGCTCCTACGTCAGCCAAATCCCAAGCGGGGGGAGCTTCTTCACCAACGAGGTCCAACTTCATGTGGGTCTGCGAAACGACGACCAGATTGGAAATGCTCATGGCGGTGTTGTTGCCACAAACCCAATTCAAGCATTGGTTGGAAATGCTCATGGAGGAGGCTCCGGCGCTACAAACCCAATTCAAGCATCGGTTGGAAATGCTCATGGCGGTGTTGCTGCTACAAACCCAATTCAAGAATCGTTTGGAAAATTGGTCAACAAAGACAATGCCACAAAGGTTGAAAAGCTCCGCCCAGACATGTCGGATGTCACTCTCACTGTAAAGGTTGTTAGTACTACGATAGTAGGTCGGAAGGGACGTCCTGGTGGCCCTCGAAGCCACATGCAATTTGCTGACTGCTTGGTTGGCGATGAAACTGGAGTGATAATCTTCAGAGCTACAAACGATCAAGTGGACTTGATGAAAGTGGGTTTGACTGTAACTCTGCAAAATGCCAAAGTTGACATGTTCAAAGATTCAATGAGGCTTGTTGTCGACAAAAGTGGCAGTGTTCTAGTTGCTGAGCCGGCCAGTTTTACTGTGAAGGAAGATAGCAACCTCTCACTCATTCAAGCATCGATTGGAAATGTCAACAAAGATAATGCCATAAAGGTTGAAAAGCTCCGCCCAGACATGTCGGATGTCACTCTCACTGTAAAGGTTGTTAGTACTAAGATAGTAGGTATTCAATTTGCTGAGTGCTTGGTTGGCGATGAAACTGGAATGATAGTCTTCAGAGCTACAAACGATCAAGTGGACTTGATGAAAGAGGGTTTGACTGTAACTCTGCAAAATGCCAAAGTTGACATGTCCGAAGATTCAATGAGGCTTGTTGTCGACGAAAGTGGCAGTGTTGAAGTTGCTGAGCCTGCCGGTTTTACTGTGAAGGAAGATAACAACCTCTCACTCATTGAATTTGAACGTGTCGATGTCGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

373

Amino Acids

39.72

Weight (kDa)

5.18

Isoelectric Point (pI)

24.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 143 - 231 3.5e-31 Single-stranded DNA binding protein Ssb-like, OB fold
OB_Ssb-like PF21473 268 - 347 3.6e-24 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018178)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0442351 RchiOBHm_Chr7g0219251
rosa_laevigata RLG00000006016
rosa_multiflora Rmu_co8418355.1_g000001 Rmu_sc0000124.1_g000049
rosa_roxburghii Rroxscaffold_5G00383220
rosa_rugosa Rorug04G0340500
rosa_samantha Rh4AG392200 Rh4BG405700 Rh4CG420800 Rh4DG398900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 502
AccI GTMKAC 2 cut(s) 675, 1017
AccII CGCG 2 cut(s) 101, 106
AccIII TCCGGA 1 cut(s) 34
AciI CCGC 5 cut(s) 165, 243, 348, 430, 805
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 1 cut(s) 1094
AcuI CTGAAG 2 cut(s) 556, 898
AcyI GRCGYC 1 cut(s) 499
AfaI GTAC 2 cut(s) 475, 850
AfiI CCNNNNNNNGG 3 cut(s) 20, 166, 167
AflIII ACRYGT 6 cut(s) 99, 438, 645, 813, 987, 1102
AjnI CCWGG 2 cut(s) 39, 502
AluBI AGCT 5 cut(s) 174, 427, 578, 802, 920
AluI AGCT 5 cut(s) 174, 427, 578, 802, 920
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 34
AoxI GGCC 6 cut(s) 23, 42, 58, 124, 508, 708
ApaI GGGCCC 1 cut(s) 128
ApeKI GCWGC 2 cut(s) 10, 356
ApoI RAATTY 1 cut(s) 1094
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 8 cut(s) 23, 37, 43, 58, 124, 125, 190, 509
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 172
AvaII GGWCC 2 cut(s) 37, 190
BaeGI GKGCMC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 904
BbvI GCAGC 2 cut(s) 22, 343
BccI CCATC 2 cut(s) 105, 109
BciT130I CCWGG 2 cut(s) 41, 504
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 695
BfoI RGCGCY 1 cut(s) 309
BisI GCNGC 2 cut(s) 11, 357
BlpI GCTNAGC 2 cut(s) 702, 1044
BlsI GCNGC 2 cut(s) 12, 358
BmcAI AGTACT 2 cut(s) 475, 850
Bme1390I CCNGG 3 cut(s) 41, 82, 504
Bme18I GGWCC 2 cut(s) 37, 190
BmgT120I GGNCC 8 cut(s) 23, 37, 43, 58, 124, 125, 190, 509
BmiI GGNNCC 2 cut(s) 126, 301
BmrFI CCNGG 3 cut(s) 41, 82, 504
BmsI GCATC 2 cut(s) 335, 764
BpiI GAAGAC 1 cut(s) 904
BpmI CTGGAG 1 cut(s) 579
Bpu1102I GCTNAGC 2 cut(s) 702, 1044
BpuMI CCSGG 1 cut(s) 82
Bsa29I ATCGAT 1 cut(s) 758
BsaHI GRCGYC 1 cut(s) 499
BsaI GGTCTC 1 cut(s) 82
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 166, 167
Bse118I RCCGGY 2 cut(s) 706, 1052
Bse1I ACTGG 3 cut(s) 562, 711, 904
BseAI TCCGGA 1 cut(s) 34
BseBI CCWGG 2 cut(s) 41, 504
BseCI ATCGAT 1 cut(s) 758
BseGI GGATG 3 cut(s) 120, 451, 826
BseLI CCNNNNNNNGG 3 cut(s) 20, 166, 167
BseMII CTCAG 4 cut(s) 108, 693, 867, 1035
BseNI ACTGG 3 cut(s) 562, 711, 904
BseRI GAGGAG 1 cut(s) 309
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 2 cut(s) 22, 343
Bsh1236I CGCG 2 cut(s) 101, 106
BshFI GGCC 6 cut(s) 25, 44, 60, 126, 510, 710
BshVI ATCGAT 1 cut(s) 758
BsiSI CCGG 5 cut(s) 35, 81, 303, 707, 1053
BslFI GGGAC 2 cut(s) 97, 510
BslI CCNNNNNNNGG 3 cut(s) 20, 166, 167
BsmAI GTCTC 1 cut(s) 82
BsmFI GGGAC 2 cut(s) 97, 510
BsnI GGCC 6 cut(s) 25, 44, 60, 126, 510, 710
Bso31I GGTCTC 1 cut(s) 82
Bsp120I GGGCCC 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 128
Bsp13I TCCGGA 1 cut(s) 34
Bsp143I GATC 3 cut(s) 88, 586, 928
Bsp1720I GCTNAGC 2 cut(s) 702, 1044
Bsp68I TCGCGA 1 cut(s) 106
BspACI CCGC 5 cut(s) 165, 243, 348, 430, 805
BspANI GGCC 6 cut(s) 25, 44, 60, 126, 510, 710
BspCNI CTCAG 4 cut(s) 107, 694, 868, 1036
BspDI ATCGAT 1 cut(s) 758
BspEI TCCGGA 1 cut(s) 34
BspFNI CGCG 2 cut(s) 101, 106
BspLI GGNNCC 2 cut(s) 126, 301
BspPI GGATC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 82
BsrFI RCCGGY 2 cut(s) 706, 1052
BsrI ACTGG 3 cut(s) 562, 711, 904
BssAI RCCGGY 2 cut(s) 706, 1052
BssMI GATC 3 cut(s) 88, 586, 928
BssNI GRCGYC 1 cut(s) 499
Bst2UI CCWGG 2 cut(s) 41, 504
Bst4CI ACNGT 6 cut(s) 460, 619, 721, 835, 961, 1063
BstACI GRCGYC 1 cut(s) 499
BstC8I GCNNGC 3 cut(s) 62, 708, 1050
BstDEI CTNAG 5 cut(s) 94, 702, 852, 876, 1044
BstF5I GGATG 3 cut(s) 120, 451, 826
BstFNI CGCG 2 cut(s) 101, 106
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 3 cut(s) 91, 589, 931
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 3 cut(s) 88, 586, 928
BstMWI GCNNNNNNNGC 6 cut(s) 171, 516, 546, 707, 888, 1049
BstNI CCWGG 2 cut(s) 41, 504
BstNSI RCATGY 5 cut(s) 442, 526, 649, 817, 991
BstSCI CCNGG 3 cut(s) 39, 80, 502
BstSLI GKGCMC 1 cut(s) 128
BstUI CGCG 2 cut(s) 101, 106
BstV1I GCAGC 2 cut(s) 22, 343
BstV2I GAAGAC 1 cut(s) 904
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 758
BsuRI GGCC 6 cut(s) 25, 44, 60, 126, 510, 710
BsuTUI ATCGAT 1 cut(s) 758
BtgZI GCGATG 2 cut(s) 564, 906
BtsCI GGATG 3 cut(s) 120, 451, 826
BtsI GCAGTG 2 cut(s) 694, 1036
BtsIMutI CAGTG 4 cut(s) 456, 694, 831, 1036
BtuMI TCGCGA 1 cut(s) 106
Cac8I GCNNGC 3 cut(s) 62, 708, 1050
CfoI GCGC 1 cut(s) 308
Cfr10I RCCGGY 2 cut(s) 706, 1052
Cfr13I GGNCC 8 cut(s) 23, 37, 43, 58, 124, 125, 190, 509
ClaI ATCGAT 1 cut(s) 758
CseI GACGC 3 cut(s) 20, 90, 107
Csp6I GTAC 2 cut(s) 474, 849
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviAII CATG 9 cut(s) 200, 239, 290, 344, 439, 523, 646, 814, 988
CviQI GTAC 2 cut(s) 474, 849
DdeI CTNAG 5 cut(s) 94, 702, 852, 876, 1044
DpnI GATC 3 cut(s) 90, 588, 930
DpnII GATC 3 cut(s) 88, 586, 928
EaeI YGGCCR 1 cut(s) 708
EciI GGCGGA 2 cut(s) 419, 794
Eco24I GRGCYC 1 cut(s) 128
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 2 cut(s) 37, 190
Eco57I CTGAAG 2 cut(s) 556, 898
EcoRII CCWGG 2 cut(s) 39, 502
EcoT38I GRGCYC 1 cut(s) 128
FaeI CATG 9 cut(s) 203, 242, 293, 347, 442, 526, 649, 817, 991
FaqI GGGAC 2 cut(s) 97, 510
FatI CATG 9 cut(s) 199, 238, 289, 343, 438, 522, 645, 813, 987
FauI CCCGC 1 cut(s) 158
FblI GTMKAC 2 cut(s) 675, 1017
Fnu4HI GCNGC 2 cut(s) 11, 357
FokI GGATG 3 cut(s) 127, 458, 833
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 2 cut(s) 11, 357
FspBI CTAG 1 cut(s) 695
GlaI GCGC 1 cut(s) 307
GluI GCNGC 2 cut(s) 11, 357
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 309
HaeIII GGCC 6 cut(s) 25, 44, 60, 126, 510, 710
HapII CCGG 5 cut(s) 35, 81, 303, 707, 1053
HgaI GACGC 3 cut(s) 20, 90, 107
HhaI GCGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 499
Hin1II CATG 9 cut(s) 203, 242, 293, 347, 442, 526, 649, 817, 991
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HincII GTYRAC 6 cut(s) 397, 643, 676, 772, 985, 1018
HindII GTYRAC 6 cut(s) 397, 643, 676, 772, 985, 1018
HinfI GANTC 3 cut(s) 377, 656, 998
HpaII CCGG 5 cut(s) 35, 81, 303, 707, 1053
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 8 cut(s) 397, 595, 643, 676, 772, 937, 985, 1018
Hpy188I TCNGA 7 cut(s) 97, 445, 492, 575, 820, 917, 994
Hpy188III TCNNGA 3 cut(s) 35, 105, 374
Hpy8I GTNNAC 8 cut(s) 397, 595, 643, 676, 772, 937, 985, 1018
Hpy99I CGWCG 3 cut(s) 106, 221, 1022
HpyAV CCTTC 5 cut(s) 49, 75, 487, 718, 1060
HpyCH4III ACNGT 6 cut(s) 460, 619, 721, 835, 961, 1063
HpyCH4IV ACGT 3 cut(s) 147, 499, 1102
HpyCH4V TGCA 3 cut(s) 526, 628, 970
HpyF10VI GCNNNNNNNGC 6 cut(s) 171, 516, 546, 707, 888, 1049
HpyF3I CTNAG 5 cut(s) 94, 702, 852, 876, 1044
HpySE526I ACGT 3 cut(s) 147, 499, 1102
Hsp92I GRCGYC 1 cut(s) 499
Hsp92II CATG 9 cut(s) 203, 242, 293, 347, 442, 526, 649, 817, 991
HspAI GCGC 1 cut(s) 306
Kpn2I TCCGGA 1 cut(s) 34
KroI GCCGGC 1 cut(s) 706
KroNI GCCGGC 1 cut(s) 708
Kzo9I GATC 3 cut(s) 88, 586, 928
LmnI GCTCC 5 cut(s) 146, 171, 305, 432, 807
Lsp1109I GCAGC 2 cut(s) 22, 343
LweI GCATC 2 cut(s) 335, 764
MaeI CTAG 1 cut(s) 695
MaeII ACGT 3 cut(s) 147, 499, 1102
MaeIII GTNAC 4 cut(s) 448, 619, 823, 961
MalI GATC 3 cut(s) 90, 588, 930
MboI GATC 3 cut(s) 88, 586, 928
MboII GAAGA 7 cut(s) 120, 169, 562, 740, 904, 1007, 1082
MfeI CAATTG 1 cut(s) 70
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 8 cut(s) 70, 264, 318, 369, 389, 527, 869, 1094
MluI ACGCGT 1 cut(s) 99
MmeI TCCRAC 6 cut(s) 217, 259, 313, 423, 470, 798
MroI TCCGGA 1 cut(s) 34
MroNI GCCGGC 1 cut(s) 706
MslI CAYNNNNRTG 2 cut(s) 243, 348
MspI CCGG 5 cut(s) 35, 81, 303, 707, 1053
MspR9I CCNGG 3 cut(s) 41, 82, 504
MunI CAATTG 1 cut(s) 70
MvaI CCWGG 2 cut(s) 41, 504
MvnI CGCG 2 cut(s) 101, 106
MwoI GCNNNNNNNGC 6 cut(s) 171, 516, 546, 707, 888, 1049
NaeI GCCGGC 1 cut(s) 708
NciI CCSGG 1 cut(s) 82
NdeII GATC 3 cut(s) 88, 586, 928
NgoMIV GCCGGC 1 cut(s) 706
NlaIII CATG 9 cut(s) 203, 242, 293, 347, 442, 526, 649, 817, 991
NlaIV GGNNCC 2 cut(s) 126, 301
NmuCI GTSAC 2 cut(s) 448, 823
NruI TCGCGA 1 cut(s) 106
NspI RCATGY 5 cut(s) 442, 526, 649, 817, 991
PciI ACATGT 4 cut(s) 438, 645, 813, 987
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PdiI GCCGGC 1 cut(s) 708
PfeI GAWTC 3 cut(s) 377, 656, 998
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 2 cut(s) 12, 358
PscI ACATGT 4 cut(s) 438, 645, 813, 987
Psp6I CCWGG 2 cut(s) 39, 502
PspGI CCWGG 2 cut(s) 39, 502
PspN4I GGNNCC 2 cut(s) 126, 301
PspOMI GGGCCC 1 cut(s) 124
PspPI GGNCC 8 cut(s) 23, 37, 43, 58, 124, 125, 190, 509
PsuI RGATCY 1 cut(s) 88
RruI TCGCGA 1 cut(s) 106
RsaI GTAC 2 cut(s) 475, 850
RsaNI GTAC 2 cut(s) 474, 849
RseI CAYNNNNRTG 2 cut(s) 243, 348
SalI GTCGAC 2 cut(s) 674, 1016
SatI GCNGC 2 cut(s) 11, 357
Sau3AI GATC 3 cut(s) 88, 586, 928
Sau96I GGNCC 8 cut(s) 23, 37, 43, 58, 124, 125, 190, 509
ScaI AGTACT 2 cut(s) 475, 850
ScrFI CCNGG 3 cut(s) 41, 82, 504
SduI GDGCHC 1 cut(s) 128
SfaNI GCATC 2 cut(s) 335, 764
SinI GGWCC 2 cut(s) 37, 190
SmiMI CAYNNNNRTG 2 cut(s) 243, 348
Sse9I AATT 8 cut(s) 70, 264, 318, 369, 389, 527, 869, 1094
SsiI CCGC 5 cut(s) 165, 243, 348, 430, 805
SspMI CTAG 1 cut(s) 695
StyD4I CCNGG 3 cut(s) 39, 80, 502
TaaI ACNGT 6 cut(s) 460, 619, 721, 835, 961, 1063
TaiI ACGT 3 cut(s) 150, 502, 1105
TaqI TCGA 7 cut(s) 132, 514, 675, 758, 1017, 1107, 1113
TasI AATT 8 cut(s) 70, 264, 318, 369, 389, 527, 869, 1094
TatI WGTACW 2 cut(s) 473, 848
TfiI GAWTC 3 cut(s) 377, 656, 998
TscAI CASTG 4 cut(s) 463, 694, 838, 1036
TseFI GTSAC 2 cut(s) 448, 823
TseI GCWGC 2 cut(s) 10, 356
Tsp45I GTSAC 2 cut(s) 448, 823
TspDTI ATGAA 5 cut(s) 188, 567, 617, 909, 959
TspRI CASTG 4 cut(s) 463, 694, 838, 1036
VpaK11BI GGWCC 2 cut(s) 37, 190
XapI RAATTY 1 cut(s) 1094
XceI RCATGY 5 cut(s) 442, 526, 649, 817, 991
XmiI GTMKAC 2 cut(s) 675, 1017
XspI CTAG 1 cut(s) 695
ZraI GACGTC 1 cut(s) 500
ZrmI AGTACT 2 cut(s) 475, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.