RLG00000009455

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018654
Physical Location & Seq
Forward (+)
53129917 .. 53132067
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009455

Sequence Viewer

Length: 573 bp
ATGGGGAGCCTCCTCGACGAAGAGGAACTTCGGCGAGGTGGGGAGCTGGTAAAGGTTTCGAATTGGATCTTGGAGATCCTGCTACGGGTTATAGAGGGTGTTTTTCCCAACTTAAAAAGGCTGGTGGTAAGTGACCTTCATGGTTTGAGATTCTTATTATCATCTTCCGTGGCTAGAAGTCTTGTACAAGTCACACATCTTCAGGTATCTGGATGTCAAACCATGGAAGAAATAGTATCAACAGAGGAATCTGATGAAAAGCATATGAATGACATGTTTTCTAAGCTACAAAATCTGGGGCTAAAAGATCTTCCAATCCTCACAAGATTCTGCTCAAGAAGTTATACTGGATTTCCATCTTTGGAGATTTCGCAACTAAAGAACTACAATGAAATTGAAGAAAAGGACCCAAGAGAAAACCTTAGGAACGCTTTCTTTGACAAAAAGGTAGTATTTCAAAGCTTGAACAGACTGTTCATCGATGGCTTGACCAAGTTAATGACATTATGGCACGGCCAACTTTCTCCAGAGAGTTTCAAAAATCTAGAGTTTGTAGTTATTAATTCGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

191

Amino Acids

21.83

Weight (kDa)

5.64

Isoelectric Point (pI)

59.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 32 - 123 9.6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 70, 74
AcoI YGGCCR 1 cut(s) 514
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 4 cut(s) 398, 458, 466, 538
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 3 cut(s) 46, 286, 462
AluI AGCT 3 cut(s) 46, 286, 462
AlwI GGATC 2 cut(s) 70, 74
AoxI GGCC 1 cut(s) 514
AseI ATTAAT 1 cut(s) 561
Asp700I GAANNNNTTC 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 406
AsuII TTCGAA 1 cut(s) 59
AvaII GGWCC 1 cut(s) 406
AxyI CCTNAGG 1 cut(s) 422
BarI GAAGNNNNNNTAC 2 cut(s) 120, 152
BccI CCATC 2 cut(s) 364, 476
BceAI ACGGC 1 cut(s) 529
BfaI CTAG 2 cut(s) 174, 545
BglII AGATCT 1 cut(s) 307
Bme18I GGWCC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 8, 408
BpmI CTGGAG 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 319
Bsa29I ATCGAT 1 cut(s) 480
BsaBI GATNNNNATC 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 168, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 352
Bse21I CCTNAGG 1 cut(s) 422
Bse8I GATNNNNATC 1 cut(s) 355
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 168, 222
BseGI GGATG 1 cut(s) 218
BseJI GATNNNNATC 1 cut(s) 355
BseLI CCNNNNNNNGG 1 cut(s) 85
BseNI ACTGG 1 cut(s) 352
BshFI GGCC 1 cut(s) 516
BshVI ATCGAT 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 85
BsnI GGCC 1 cut(s) 516
Bsp119I TTCGAA 1 cut(s) 59
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 3 cut(s) 66, 75, 307
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 1 cut(s) 516
BspDI ATCGAT 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 8, 408
BspPI GGATC 2 cut(s) 70, 74
BspT104I TTCGAA 1 cut(s) 59
BsrGI TGTACA 1 cut(s) 184
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 168, 222
BssMI GATC 3 cut(s) 66, 75, 307
BssT1I CCWWGG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 474
Bst6I CTCTTC 1 cut(s) 15
BstAUI TGTACA 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 59
BstDEI CTNAG 2 cut(s) 282, 422
BstDSI CCRYGG 2 cut(s) 168, 222
BstF5I GGATG 1 cut(s) 218
BstKTI GATC 3 cut(s) 69, 78, 310
BstMBI GATC 3 cut(s) 66, 75, 307
BstNSI RCATGY 1 cut(s) 277
BstX2I RGATCY 3 cut(s) 66, 75, 307
BstYI RGATCY 3 cut(s) 66, 75, 307
Bsu15I ATCGAT 1 cut(s) 480
Bsu36I CCTNAGG 1 cut(s) 422
BsuRI GGCC 1 cut(s) 516
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 2 cut(s) 168, 222
BtsCI GGATG 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 480
Csp6I GTAC 1 cut(s) 185
CviAII CATG 3 cut(s) 140, 223, 274
CviJI RGCY 9 cut(s) 9, 46, 121, 173, 286, 301, 462, 486, 516
CviKI_1 RGCY 9 cut(s) 9, 46, 121, 173, 286, 301, 462, 486, 516
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 2 cut(s) 282, 422
DpnI GATC 3 cut(s) 68, 77, 309
DpnII GATC 3 cut(s) 66, 75, 307
EaeI YGGCCR 1 cut(s) 514
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 406
Eco57I CTGAAG 1 cut(s) 185
Eco81I CCTNAGG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 3 cut(s) 143, 226, 277
FaiI YATR 8 cut(s) 92, 141, 224, 264, 266, 275, 345, 508
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FatI CATG 3 cut(s) 139, 222, 273
FauNDI CATATG 1 cut(s) 264
FokI GGATG 1 cut(s) 225
FspBI CTAG 2 cut(s) 174, 545
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 1 cut(s) 516
Hin1II CATG 3 cut(s) 143, 226, 277
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 3 cut(s) 150, 248, 327
Hpy188I TCNGA 1 cut(s) 253
Hpy188III TCNNGA 4 cut(s) 210, 336, 527, 545
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 146
HpyCH4III ACNGT 1 cut(s) 474
HpyF3I CTNAG 2 cut(s) 282, 422
Hsp92II CATG 3 cut(s) 143, 226, 277
Kzo9I GATC 3 cut(s) 66, 75, 307
LmnI GCTCC 2 cut(s) 6, 43
LpnPI CCDG 8 cut(s) 32, 92, 107, 188, 195, 281, 333, 540
MaeI CTAG 2 cut(s) 174, 545
MaeIII GTNAC 2 cut(s) 131, 190
MalI GATC 3 cut(s) 68, 77, 309
MboI GATC 3 cut(s) 66, 75, 307
MboII GAAGA 6 cut(s) 32, 156, 191, 239, 302, 410
MflI RGATCY 3 cut(s) 66, 75, 307
MluCI AATT 3 cut(s) 61, 393, 562
MnlI CCTC 7 cut(s) 16, 20, 23, 29, 88, 238, 329
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 3 cut(s) 113, 497, 561
MslI CAYNNNNRTG 1 cut(s) 267
NcoI CCATGG 1 cut(s) 222
NdeI CATATG 1 cut(s) 264
NdeII GATC 3 cut(s) 66, 75, 307
NlaIII CATG 3 cut(s) 143, 226, 277
NlaIV GGNNCC 2 cut(s) 8, 408
NmuCI GTSAC 2 cut(s) 131, 190
NspI RCATGY 1 cut(s) 277
NspV TTCGAA 1 cut(s) 59
PciI ACATGT 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 3 cut(s) 150, 248, 327
PpuMI RGGWCCY 1 cut(s) 406
PscI ACATGT 1 cut(s) 273
PshBI ATTAAT 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 406
PspN4I GGNNCC 2 cut(s) 8, 408
PspPI GGNCC 1 cut(s) 406
PspPPI RGGWCCY 1 cut(s) 406
PsuI RGATCY 3 cut(s) 66, 75, 307
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 3 cut(s) 113, 497, 561
Sau3AI GATC 3 cut(s) 66, 75, 307
Sau96I GGNCC 1 cut(s) 406
SetI ASST 9 cut(s) 40, 48, 57, 138, 207, 288, 423, 450, 464
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 334
SmoI CTYRAG 1 cut(s) 334
Sse9I AATT 3 cut(s) 61, 393, 562
SspMI CTAG 2 cut(s) 174, 545
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 474
TaqI TCGA 3 cut(s) 15, 59, 480
TasI AATT 3 cut(s) 61, 393, 562
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 3 cut(s) 150, 248, 327
Tru1I TTAA 3 cut(s) 113, 497, 561
Tru9I TTAA 3 cut(s) 113, 497, 561
TseFI GTSAC 2 cut(s) 131, 190
Tsp45I GTSAC 2 cut(s) 131, 190
TspDTI ATGAA 5 cut(s) 128, 270, 281, 405, 466
TspGWI ACGGA 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 406
VspI ATTAAT 1 cut(s) 561
XbaI TCTAGA 1 cut(s) 544
XceI RCATGY 1 cut(s) 277
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 2 cut(s) 174, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.