RLG00000010586

Staphylococcal nuclease domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018417
Physical Location & Seq
Reverse (-)
2799258 .. 2801903
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010586

Sequence Viewer

Length: 2646 bp
ATGGCATCACAATGGTATAGAGGCAGAGTCAAGGCTGTTCCTTCAGGCGATTCCCTTGTCATCCAGCTTCTTCAGTCAAACAAGACCAGATTCCAACTGGAAATTACTTTGTTATCAGTTCGTGCTCCCAAACTGGCCGGTAAAGATCGCAGTACTGATGAGCCTTTTGCATGGGACAGCAGAGAGTGTCTGAGGAAGCTCTGCCTCGGAAGGGAGGTGGTTTTCAGCACTGATAACATTGAAGTAGCGAAGGGCGTCGGCTCTGTTTTCCTTGAAAACAATAATGTTGCAGCCCTTGTTGTTCAAAAAGGGTGGGCAGCTGTCACGTCACCGAGCACAACTCCTTACAATTGTGATCTGCAGTTTCACATGGATCAAGCCTTTAATAAGGGTCTTGGTCGCTGGAGCACGGTTCCAGGTGCCGCAGAGGCATCCATTAGGACTCTACCTCCCCAACTCAACTTCGAGACCATGAAGACTCTGTTGGCTGCAAAGAAGGGCAAGCCCATAGAAGCTATTGTCGAGCAGGTTGTTGATGGCAGTTTTGTTCGGGCTCACTTGCTTCCGGAGTTTCAGTTTGTCGAAGTCATTGTTGCAGGAACTCAGGCGCCCTACATTGACCCTAATGCTTATCCTTTCGCACTAGAAGCTAAGTTTTTCACAGAGTCTCGTGTGTTGAATAGAGATGTGCATATTGTTCTGGAAGGTGTTCATAAGTTCAACAAGTTCAGCCGTCTAATTGGCTCTGTGTATTACCCCGATGGAGACTCCGAAAAGGACTTGGGCCTGGAGCTTGTGGAGAATGGTTATGCTAAATATGTACCATGGAGCGCGAAAGTGATGGATGAAGATGCAAGCCGGCGGCTCAAGACTGCAGAGCTTGAAGCTAAGAAAAACAAGCTGAGGATTTGGACACACTTTGTACCCCCAGCTACAAATTCCATGCCAATCCATGACCAGAATTTCACAGGAACAGTTGTGGAGGTTGTAAGTGGGGAATGTGTCATTGTGGCTGATGATTCTGGAGAGCGGAGAGTCAATCTTTCAAGTATTTGGTGTCCGAAGATGGGAAATCCTCGTAGAGGTGATGAGCCAGCGGCATATGCACGTGAAGCCAAGGAGTTGCTGAGGACACGCCTTATTGGACGCCAAGTGAATGTTCAAATGGAGTATTCAAGGAAGGTCGGCCAAGCAGATGCAGATTCTTCCAGAGTAATAGATTTCGGATCAGTCTTTATTAAGGCTGAGGGGGAAGATAAAAGCAACCAGGCTGAGGAAAACGTTGCTGAGCTTGTGGTTGCATGTGGCTTCGGCACTGTTATCAAACACCGAGATTTTGAGGAGAGATCAAGATACTATGATGTCCTTCTTGCTGCCGAATCAGGTGCTATTGCTGGAAGGAAAGGACTACATTGTTCCAAGGAGGCCCCCGTTGTGCACATGACAGACTTGACAATGGCAACGGCCAAGAAAGCAAGAGACGTCTTGCCATCCTTGCAGCGAAGTAGGAGAGTTTCTGCAGTTGTGGAATATGTCCTCAGCGGTCATCGTTTTAAACTAAACATTCCCAAGGAGACATGCAGCATTTCCTTCTCTCTCTCCGGTGTGAGATGTCCCGGTCGCGATGAGCCCTATTCTGATGAAGCAATTGCAGTAATGAGGCGAAGATTAATGCAGAGAGATGTTGAGATTGAAGTGGAGACTGTTGATAGAAACGGAACTTTCTTGGGAACTCTATGGGAGTCGGGGACCAATGTGTCAATTGCCCTCGTTGAAGCTGGCCTTGCCAAATATATTCCCTTTGGTGGCAGTGACAAGGTCCAAGATGGCCACCTTCTTGAACGAGCTATGAAAAATGCAAAAACGAAGAAGCTGAAAATTTGGGAAAATTTTGAAGGAAAACCCAACAAACAGAAAGAACTAAAGGTAGTGATTACAGAAGTTTTGGACAGAGGAAAATTTTATGTGCAGACAATTGAGGACGAGAAGAGTGCCTCTATTCAGCAACAGCTTATTTCTTTGAACCTTGAAGAAGCTCCTTCTGGAAGTGATGCTTTTAATCCTAGGGAGGGTGACATTGTCCTTGCTCAGTTTAGTGCAGATAATTCTTGGAACCGAGCTAGGATTGTCAATGTGCGAAAAGACAAGTTTGAAGTGTTCTACATTGACTATGGTAATCAAGAGGTTGTCCCTTACAGTCAGTTACGACCTCTTAACTCTTCTGCTGCCTCTGCACCTGGTCTTGCTCAGCTATGCAGCCTTGCACATGTTACTGTCCCGAGCTTGGAGGAGGAGTTTGGTCAAGAAGCAGGAGAGTATTTGAGTGAGTGCACATTGGACAAAGAGTTCACAGCCGTGATTGAGGGAAGGGATATTTCCGGAGGAAAATTTGCTAAAGGCCAGGGAACCGGACCAGCTCTTATGGTAACTCTCTTCGCGGTAGATAATGCTGACAGTATAAATGCTGCCATGCTTCAGAATGGACTTGCTAGTCTAGATAACCAGAAGAGGTCTTGGAAGTCTCGTACCTTGAGAGAGTTCCAGGAAAAAGCACAAACCTTCCGCCAGGGGATGTGGCATGATTATTACGAGTCTGATCATCATGAGAACAGAGCTCCTCCTGCAAGAAAGGCTGCTGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000932 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009266 GO:0009408 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010371 GO:0010372 GO:0010467 GO:0010468 GO:0010494 GO:0010565 GO:0010605 GO:0010629 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016070 GO:0016071 GO:0016101 GO:0016102 GO:0016114 GO:0016787 GO:0016788 GO:0019216 GO:0019222 GO:0019439 GO:0019747 GO:0019752 GO:0030054 GO:0030312 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0043455 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046394 GO:0046483 GO:0046686 GO:0046700 GO:0046885 GO:0046886 GO:0046889 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060255 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090305 GO:0090351 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05169 ko05203 map05169 map05203
Pfam Domains
Protein Families
SNase TUDOR

Protein Analysis

882

Amino Acids

98.0

Weight (kDa)

6.62

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNase PF00565 35 - 136 1.6e-06 Staphylococcal nuclease homologue
SNase PF00565 209 - 305 1.2e-11 Staphylococcal nuclease homologue
SNase PF00565 531 - 630 7.5e-15 Staphylococcal nuclease homologue
TUDOR PF00567 637 - 756 5.7e-24 Tudor domain
SNase PF00565 762 - 861 1.3e-07 Staphylococcal nuclease homologue
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018417)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g14720
rosa_chinensis RchiOBHm_Chr6g0309151
rosa_laevigata RLG00000010586
rosa_multiflora Rmu_sc0020102.1_g000004
rosa_rugosa Rorug06G0370100
rosa_samantha Rh6AG482900 Rh6BG492000 Rh6CG496700 Rh6DG483600
rosa_wichuraiana Rw6G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1756, 2490
AatII GACGTC 1 cut(s) 1485
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 2 cut(s) 419, 607
AccBSI CCGCTC 1 cut(s) 1030
AccII CGCG 3 cut(s) 833, 1623, 2438
AccIII TCCGGA 2 cut(s) 565, 2378
AciI CCGC 7 cut(s) 423, 862, 1030, 1097, 1542, 2438, 2563
AclI AACGTT 1 cut(s) 1281
AclWI GGATC 2 cut(s) 381, 1234
AcoI YGGCCR 4 cut(s) 135, 1186, 1464, 1828
AcsI RAATTY 6 cut(s) 937, 961, 1878, 1888, 1958, 2387
AcuI CTGAAG 3 cut(s) 27, 56, 2459
AcvI CACGTG 1 cut(s) 1109
AcyI GRCGYC 4 cut(s) 255, 608, 1147, 1482
AfaI GTAC 4 cut(s) 154, 822, 924, 2527
AfiI CCNNNNNNNGG 7 cut(s) 211, 1067, 1082, 1273, 1805, 2069, 2284
AflIII ACRYGT 1 cut(s) 2266
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 7 cut(s) 415, 786, 1266, 2236, 2400, 2541, 2565
AjuI GAANNNNNNNTTGG 4 cut(s) 467, 499, 1143, 1175
AleI CACNNNNGTG 1 cut(s) 2354
AloI GAACNNNNNNTCC 2 cut(s) 2330, 2362
Alw21I GWGCWC 6 cut(s) 127, 338, 410, 1440, 2333, 2617
Alw26I GTCTC 7 cut(s) 461, 672, 759, 1473, 1568, 1694, 2526
Alw44I GTGCAC 2 cut(s) 1436, 2329
AlwI GGATC 2 cut(s) 381, 1234
Ama87I CYCGRG 1 cut(s) 2278
Aor13HI TCCGGA 2 cut(s) 565, 2378
AoxI GGCC 8 cut(s) 135, 784, 1186, 1425, 1464, 1780, 1828, 2398
ApaLI GTGCAC 2 cut(s) 1436, 2329
ApoI RAATTY 6 cut(s) 937, 961, 1878, 1888, 1958, 2387
AseI ATTAAT 1 cut(s) 1670
Asp700I GAANNNNTTC 1 cut(s) 708
AspA2I CCTAGG 1 cut(s) 2063
AspLEI GCGC 2 cut(s) 610, 833
AspS9I GGNCC 5 cut(s) 784, 1426, 1749, 1819, 2411
AsuC2I CCSGG 1 cut(s) 1617
AsuHPI GGTGA 3 cut(s) 321, 1097, 2084
AvaI CYCGRG 1 cut(s) 2278
AvaII GGWCC 3 cut(s) 1749, 1819, 2411
AvrII CCTAGG 1 cut(s) 2063
BaeGI GKGCMC 2 cut(s) 1440, 2333
BalI TGGCCA 1 cut(s) 1830
BanI GGYRCC 2 cut(s) 419, 607
BanII GRGCYC 3 cut(s) 556, 1632, 2617
BauI CACGAG 1 cut(s) 669
BbrPI CACGTG 1 cut(s) 1109
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 6 cut(s) 127, 338, 410, 1440, 2333, 2617
BbvCI CCTCAGC 5 cut(s) 902, 1127, 1245, 1272, 1538
BccI CCATC 6 cut(s) 530, 755, 835, 1060, 1498, 1820
BceAI ACGGC 3 cut(s) 717, 1479, 2339
BcgI CGANNNNNNTGC 4 cut(s) 1367, 1401, 1973, 2007
BciT130I CCWGG 7 cut(s) 417, 788, 1268, 2238, 2402, 2543, 2567
BclI TGATCA 1 cut(s) 2596
BcnI CCSGG 1 cut(s) 1617
BcoDI GTCTC 7 cut(s) 461, 672, 759, 1473, 1568, 1694, 2526
BfaI CTAG 5 cut(s) 644, 2064, 2121, 2490, 2495
BfmI CTRYAG 3 cut(s) 359, 873, 1518
BfoI RGCGCY 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 517
BglI GCCNNNNNGGC 1 cut(s) 428
BlnI CCTAGG 1 cut(s) 2063
BlpI GCTNAGC 2 cut(s) 1287, 2247
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 8 cut(s) 417, 788, 1268, 1617, 2238, 2402, 2543, 2567
Bme18I GGWCC 3 cut(s) 1749, 1819, 2411
BmeT110I CYCGRG 1 cut(s) 2278
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 5 cut(s) 784, 1426, 1749, 1819, 2411
BmiI GGNNCC 7 cut(s) 414, 421, 609, 1428, 1750, 2114, 2407
BmrFI CCNGG 8 cut(s) 417, 788, 1268, 1617, 2238, 2402, 2543, 2567
BmsI GCATC 5 cut(s) 14, 440, 841, 1186, 2041
BpiI GAAGAC 1 cut(s) 482
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 3 cut(s) 424, 809, 1044
Bpu10I CCTNAGC 5 cut(s) 902, 1127, 1245, 1272, 1538
Bpu1102I GCTNAGC 2 cut(s) 1287, 2247
BpuEI CTTGAG 2 cut(s) 851, 2551
BpuMI CCSGG 1 cut(s) 1617
BsaAI YACGTR 1 cut(s) 1109
BsaHI GRCGYC 4 cut(s) 255, 608, 1147, 1482
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 8 cut(s) 205, 824, 1116, 1419, 1569, 2063, 2401, 2566
BsaWI WCCGGW 4 cut(s) 565, 1601, 2378, 2408
BsaXI ACNNNNNCTCC 6 cut(s) 433, 463, 560, 590, 2279, 2309
Bsc4I CCNNNNNNNGG 7 cut(s) 211, 1067, 1082, 1273, 1805, 2069, 2284
Bse118I RCCGGY 2 cut(s) 137, 858
Bse1I ACTGG 2 cut(s) 102, 138
BseAI TCCGGA 2 cut(s) 565, 2378
BseBI CCWGG 7 cut(s) 417, 788, 1268, 2238, 2402, 2543, 2567
BseDI CCNNGG 8 cut(s) 205, 824, 1116, 1419, 1569, 2063, 2401, 2566
BseGI GGATG 5 cut(s) 60, 431, 850, 1490, 2577
BseLI CCNNNNNNNGG 7 cut(s) 211, 1067, 1082, 1273, 1805, 2069, 2284
BseNI ACTGG 2 cut(s) 102, 138
BseRI GAGGAG 4 cut(s) 1355, 2303, 2306, 2607
BseSI GKGCMC 2 cut(s) 1440, 2333
BseYI CCCAGC 1 cut(s) 928
BsgI GTGCAG 3 cut(s) 1988, 2118, 2217
Bsh1236I CGCG 3 cut(s) 833, 1623, 2438
Bsh1285I CGRYCG 1 cut(s) 1621
BshFI GGCC 8 cut(s) 137, 786, 1188, 1427, 1466, 1782, 1830, 2400
BshNI GGYRCC 2 cut(s) 419, 607
BsiEI CGRYCG 1 cut(s) 1621
BsiHKAI GWGCWC 6 cut(s) 127, 338, 410, 1440, 2333, 2617
BsiHKCI CYCGRG 1 cut(s) 2278
BsiSI CCGG 7 cut(s) 138, 566, 859, 1602, 1617, 2379, 2409
BslFI GGGAC 5 cut(s) 188, 1599, 1762, 2174, 2261
BslI CCNNNNNNNGG 7 cut(s) 211, 1067, 1082, 1273, 1805, 2069, 2284
BsmAI GTCTC 7 cut(s) 461, 672, 759, 1473, 1568, 1694, 2526
BsmBI CGTCTC 1 cut(s) 1473
BsmFI GGGAC 5 cut(s) 188, 1599, 1762, 2174, 2261
BsnI GGCC 8 cut(s) 137, 786, 1188, 1427, 1466, 1782, 1830, 2400
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 2278
Bsp1286I GDGCHC 8 cut(s) 127, 338, 410, 556, 1440, 1632, 2333, 2617
Bsp13I TCCGGA 2 cut(s) 565, 2378
Bsp143I GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
Bsp1720I GCTNAGC 2 cut(s) 1287, 2247
Bsp19I CCATGG 1 cut(s) 824
Bsp68I TCGCGA 1 cut(s) 1623
BspACI CCGC 7 cut(s) 423, 862, 1030, 1097, 1542, 2438, 2563
BspANI GGCC 8 cut(s) 137, 786, 1188, 1427, 1466, 1782, 1830, 2400
BspEI TCCGGA 2 cut(s) 565, 2378
BspFNI CGCG 3 cut(s) 833, 1623, 2438
BspHI TCATGA 1 cut(s) 2602
BspLI GGNNCC 7 cut(s) 414, 421, 609, 1428, 1750, 2114, 2407
BspMAI CTGCAG 3 cut(s) 363, 877, 1522
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 2 cut(s) 381, 1234
BspT107I GGYRCC 2 cut(s) 419, 607
BspTNI GGTCTC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 1030
BsrFI RCCGGY 2 cut(s) 137, 858
BsrI ACTGG 2 cut(s) 102, 138
BssAI RCCGGY 2 cut(s) 137, 858
BssECI CCNNGG 8 cut(s) 205, 824, 1116, 1419, 1569, 2063, 2401, 2566
BssMI GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
BssNI GRCGYC 4 cut(s) 255, 608, 1147, 1482
BssSI CACGAG 1 cut(s) 669
BssT1I CCWWGG 5 cut(s) 824, 1116, 1419, 1569, 2063
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 7 cut(s) 417, 788, 1268, 2238, 2402, 2543, 2567
Bst4CI ACNGT 7 cut(s) 412, 976, 1318, 1705, 2198, 2275, 2456
Bst6I CTCTTC 4 cut(s) 1982, 2224, 2438, 2501
BstACI GRCGYC 4 cut(s) 255, 608, 1147, 1482
BstBAI YACGTR 1 cut(s) 1109
BstC8I GCNNGC 6 cut(s) 503, 856, 860, 1095, 1780, 2638
BstDSI CCRYGG 1 cut(s) 824
BstENI CCTNNNNNAGG 1 cut(s) 1080
BstF5I GGATG 5 cut(s) 60, 431, 850, 1490, 2577
BstFNI CGCG 3 cut(s) 833, 1623, 2438
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 2 cut(s) 610, 833
BstKTI GATC 6 cut(s) 148, 358, 376, 1229, 1349, 2599
BstMAI GTCTC 7 cut(s) 461, 672, 759, 1473, 1568, 1694, 2526
BstMBI GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
BstMCI CGRYCG 1 cut(s) 1621
BstNI CCWGG 7 cut(s) 417, 788, 1268, 2238, 2402, 2543, 2567
BstNSI RCATGY 3 cut(s) 1305, 1581, 2270
BstSCI CCNGG 8 cut(s) 415, 786, 1266, 1615, 2236, 2400, 2541, 2565
BstSFI CTRYAG 3 cut(s) 359, 873, 1518
BstSLI GKGCMC 2 cut(s) 1440, 2333
BstUI CGCG 3 cut(s) 833, 1623, 2438
BstV2I GAAGAC 1 cut(s) 482
BsuRI GGCC 8 cut(s) 137, 786, 1188, 1427, 1466, 1782, 1830, 2400
BtgI CCRYGG 1 cut(s) 824
BtgZI GCGATG 1 cut(s) 1638
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 5 cut(s) 60, 431, 850, 1490, 2577
BtsI GCAGTG 1 cut(s) 1816
BtsIMutI CAGTG 3 cut(s) 228, 1314, 1816
BtuMI TCGCGA 1 cut(s) 1623
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 6 cut(s) 503, 856, 860, 1095, 1780, 2638
CciI TCATGA 1 cut(s) 2602
CfoI GCGC 2 cut(s) 610, 833
Cfr10I RCCGGY 2 cut(s) 137, 858
Cfr13I GGNCC 5 cut(s) 784, 1426, 1749, 1819, 2411
CseI GACGC 2 cut(s) 244, 1155
CsiI ACCWGGT 1 cut(s) 2236
Csp6I GTAC 4 cut(s) 153, 821, 923, 2526
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
CviQI GTAC 4 cut(s) 153, 821, 923, 2526
DinI GGCGCC 1 cut(s) 609
DpnI GATC 6 cut(s) 147, 357, 375, 1228, 1348, 2598
DpnII GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
DraI TTTAAA 1 cut(s) 1555
DrdI GACNNNNNNGTC 2 cut(s) 1756, 2490
DseDI GACNNNNNNGTC 2 cut(s) 1756, 2490
EaeI YGGCCR 4 cut(s) 135, 1186, 1464, 1828
Eam1104I CTCTTC 4 cut(s) 1982, 2224, 2438, 2501
EarI CTCTTC 4 cut(s) 1982, 2224, 2438, 2501
EciI GGCGGA 1 cut(s) 2552
Ecl136II GAGCTC 1 cut(s) 2615
Eco130I CCWWGG 5 cut(s) 824, 1116, 1419, 1569, 2063
Eco24I GRGCYC 3 cut(s) 556, 1632, 2617
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 3 cut(s) 1749, 1819, 2411
Eco53kI GAGCTC 1 cut(s) 2615
Eco57I CTGAAG 3 cut(s) 27, 56, 2459
Eco72I CACGTG 1 cut(s) 1109
Eco88I CYCGRG 1 cut(s) 2278
EcoICRI GAGCTC 1 cut(s) 2615
EcoNI CCTNNNNNAGG 1 cut(s) 1080
EcoO109I RGGNCCY 1 cut(s) 1426
EcoRII CCWGG 7 cut(s) 415, 786, 1266, 2236, 2400, 2541, 2565
EcoT14I CCWWGG 5 cut(s) 824, 1116, 1419, 1569, 2063
EcoT38I GRGCYC 3 cut(s) 556, 1632, 2617
EgeI GGCGCC 1 cut(s) 609
EheI GGCGCC 1 cut(s) 609
ErhI CCWWGG 5 cut(s) 824, 1116, 1419, 1569, 2063
Esp3I CGTCTC 1 cut(s) 1473
FalI AAGNNNNNCTT 4 cut(s) 1767, 1799, 2038, 2070
FaqI GGGAC 5 cut(s) 188, 1599, 1762, 2174, 2261
FauNDI CATATG 1 cut(s) 1102
FbaI TGATCA 1 cut(s) 2596
FokI GGATG 5 cut(s) 47, 418, 857, 1477, 2584
FriOI GRGCYC 3 cut(s) 556, 1632, 2617
FspBI CTAG 5 cut(s) 644, 2064, 2121, 2490, 2495
GlaI GCGC 2 cut(s) 609, 832
GsaI CCCAGC 1 cut(s) 932
GsuI CTGGAG 3 cut(s) 424, 809, 1044
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 8 cut(s) 137, 786, 1188, 1427, 1466, 1782, 1830, 2400
HapII CCGG 7 cut(s) 138, 566, 859, 1602, 1617, 2379, 2409
HgaI GACGC 2 cut(s) 244, 1155
HhaI GCGC 2 cut(s) 610, 833
Hin1I GRCGYC 4 cut(s) 255, 608, 1147, 1482
Hin6I GCGC 2 cut(s) 608, 831
HinP1I GCGC 2 cut(s) 608, 831
HpaII CCGG 7 cut(s) 138, 566, 859, 1602, 1617, 2379, 2409
HphI GGTGA 3 cut(s) 321, 1097, 2084
Hpy166II GTNNAC 3 cut(s) 1438, 2331, 2349
Hpy188I TCNGA 8 cut(s) 192, 209, 772, 1062, 1226, 1639, 2478, 2596
Hpy8I GTNNAC 3 cut(s) 1438, 2331, 2349
Hpy99I CGWCG 1 cut(s) 260
HpyCH4III ACNGT 7 cut(s) 412, 976, 1318, 1705, 2198, 2275, 2456
HpyCH4IV ACGT 4 cut(s) 326, 1108, 1281, 1482
HpySE526I ACGT 4 cut(s) 326, 1108, 1281, 1482
Hsp92I GRCGYC 4 cut(s) 255, 608, 1147, 1482
HspAI GCGC 2 cut(s) 608, 831
KasI GGCGCC 1 cut(s) 607
Kpn2I TCCGGA 2 cut(s) 565, 2378
KroI GCCGGC 1 cut(s) 858
KroNI GCCGGC 1 cut(s) 860
Ksp22I TGATCA 1 cut(s) 2596
Kzo9I GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
LmnI GCTCC 6 cut(s) 130, 405, 790, 828, 2041, 2620
LweI GCATC 5 cut(s) 14, 440, 841, 1186, 2041
MabI ACCWGGT 1 cut(s) 2236
MaeI CTAG 5 cut(s) 644, 2064, 2121, 2490, 2495
MaeII ACGT 4 cut(s) 326, 1108, 1281, 1482
MaeIII GTNAC 7 cut(s) 322, 327, 1811, 2072, 2202, 2269, 2425
MalI GATC 6 cut(s) 147, 357, 375, 1228, 1348, 2598
MbiI CCGCTC 1 cut(s) 1030
MboI GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
MfeI CAATTG 4 cut(s) 349, 1647, 1761, 1974
MhlI GDGCHC 8 cut(s) 127, 338, 410, 556, 1440, 1632, 2333, 2617
MlsI TGGCCA 1 cut(s) 1830
MluNI TGGCCA 1 cut(s) 1830
Mly113I GGCGCC 1 cut(s) 608
MlyI GAGTC 8 cut(s) 36, 436, 472, 674, 761, 1044, 1751, 2600
MmeI TCCRAC 1 cut(s) 118
Mox20I TGGCCA 1 cut(s) 1830
MroI TCCGGA 2 cut(s) 565, 2378
MroNI GCCGGC 1 cut(s) 858
MroXI GAANNNNTTC 1 cut(s) 708
MscI TGGCCA 1 cut(s) 1830
MseI TTAA 6 cut(s) 384, 1239, 1554, 1670, 2058, 2214
MslI CAYNNNNRTG 2 cut(s) 10, 2354
Msp20I TGGCCA 1 cut(s) 1830
MspA1I CMGCKG 3 cut(s) 320, 1097, 1542
MspI CCGG 7 cut(s) 138, 566, 859, 1602, 1617, 2379, 2409
MspR9I CCNGG 8 cut(s) 417, 788, 1268, 1617, 2238, 2402, 2543, 2567
MunI CAATTG 4 cut(s) 349, 1647, 1761, 1974
MvaI CCWGG 7 cut(s) 417, 788, 1268, 2238, 2402, 2543, 2567
MvnI CGCG 3 cut(s) 833, 1623, 2438
NaeI GCCGGC 1 cut(s) 860
NarI GGCGCC 1 cut(s) 608
NciI CCSGG 1 cut(s) 1617
NcoI CCATGG 1 cut(s) 824
NdeI CATATG 1 cut(s) 1102
NdeII GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
NgoMIV GCCGGC 1 cut(s) 858
NlaIV GGNNCC 7 cut(s) 414, 421, 609, 1428, 1750, 2114, 2407
NmuCI GTSAC 4 cut(s) 322, 327, 1811, 2072
NruI TCGCGA 1 cut(s) 1623
NspI RCATGY 3 cut(s) 1305, 1581, 2270
OliI CACNNNNGTG 1 cut(s) 2354
PagI TCATGA 1 cut(s) 2602
PciI ACATGT 1 cut(s) 2266
PdiI GCCGGC 1 cut(s) 860
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 5 cut(s) 50, 90, 1019, 1202, 1379
PflFI GACNNNGTC 2 cut(s) 1817, 2078
PfoI TCCNGGA 1 cut(s) 2541
PleI GAGTC 8 cut(s) 35, 436, 472, 673, 761, 1043, 1750, 2599
PluTI GGCGCC 1 cut(s) 611
PmaCI CACGTG 1 cut(s) 1109
PmlI CACGTG 1 cut(s) 1109
PpsI GAGTC 8 cut(s) 35, 436, 472, 673, 761, 1043, 1750, 2599
Ppu21I YACGTR 1 cut(s) 1109
PscI ACATGT 1 cut(s) 2266
PshBI ATTAAT 1 cut(s) 1670
Psp124BI GAGCTC 1 cut(s) 2617
Psp1406I AACGTT 1 cut(s) 1281
Psp6I CCWGG 7 cut(s) 415, 786, 1266, 2236, 2400, 2541, 2565
PspCI CACGTG 1 cut(s) 1109
PspFI CCCAGC 1 cut(s) 928
PspGI CCWGG 7 cut(s) 415, 786, 1266, 2236, 2400, 2541, 2565
PspN4I GGNNCC 7 cut(s) 414, 421, 609, 1428, 1750, 2114, 2407
PspPI GGNCC 5 cut(s) 784, 1426, 1749, 1819, 2411
PstI CTGCAG 3 cut(s) 363, 877, 1522
PsyI GACNNNGTC 2 cut(s) 1817, 2078
PvuII CAGCTG 1 cut(s) 320
RruI TCGCGA 1 cut(s) 1623
RsaI GTAC 4 cut(s) 154, 822, 924, 2527
RsaNI GTAC 4 cut(s) 153, 821, 923, 2526
RseI CAYNNNNRTG 2 cut(s) 10, 2354
SacI GAGCTC 1 cut(s) 2617
SaqAI TTAA 6 cut(s) 384, 1239, 1554, 1670, 2058, 2214
Sau3AI GATC 6 cut(s) 145, 355, 373, 1226, 1346, 2596
Sau96I GGNCC 5 cut(s) 784, 1426, 1749, 1819, 2411
ScaI AGTACT 1 cut(s) 154
SchI GAGTC 8 cut(s) 36, 436, 472, 674, 761, 1044, 1751, 2600
ScrFI CCNGG 8 cut(s) 417, 788, 1268, 1617, 2238, 2402, 2543, 2567
SduI GDGCHC 8 cut(s) 127, 338, 410, 556, 1440, 1632, 2333, 2617
SexAI ACCWGGT 1 cut(s) 2236
SfaNI GCATC 5 cut(s) 14, 440, 841, 1186, 2041
SfcI CTRYAG 3 cut(s) 359, 873, 1518
SfoI GGCGCC 1 cut(s) 609
SinI GGWCC 3 cut(s) 1749, 1819, 2411
SmiMI CAYNNNNRTG 2 cut(s) 10, 2354
SmlI CTYRAG 2 cut(s) 866, 2530
SmoI CTYRAG 2 cut(s) 866, 2530
SsiI CCGC 7 cut(s) 423, 862, 1030, 1097, 1542, 2438, 2563
SspDI GGCGCC 1 cut(s) 607
SspMI CTAG 5 cut(s) 644, 2064, 2121, 2490, 2495
SstI GAGCTC 1 cut(s) 2617
StyD4I CCNGG 8 cut(s) 415, 786, 1266, 1615, 2236, 2400, 2541, 2565
StyI CCWWGG 5 cut(s) 824, 1116, 1419, 1569, 2063
TaaI ACNGT 7 cut(s) 412, 976, 1318, 1705, 2198, 2275, 2456
TaiI ACGT 4 cut(s) 329, 1111, 1284, 1485
TaqI TCGA 3 cut(s) 465, 522, 582
TatI WGTACW 1 cut(s) 152
TauI GCSGC 3 cut(s) 425, 865, 1100
TfiI GAWTC 5 cut(s) 50, 90, 1019, 1202, 1379
Tru1I TTAA 6 cut(s) 384, 1239, 1554, 1670, 2058, 2214
Tru9I TTAA 6 cut(s) 384, 1239, 1554, 1670, 2058, 2214
TscAI CASTG 3 cut(s) 235, 1321, 1816
TseFI GTSAC 4 cut(s) 322, 327, 1811, 2072
Tsp45I GTSAC 4 cut(s) 322, 327, 1811, 2072
TspDTI ATGAA 5 cut(s) 488, 701, 861, 1656, 1865
TspGWI ACGGA 1 cut(s) 1731
TspRI CASTG 3 cut(s) 235, 1321, 1816
Tth111I GACNNNGTC 2 cut(s) 1817, 2078
VneI GTGCAC 2 cut(s) 1436, 2329
VpaK11BI GGWCC 3 cut(s) 1749, 1819, 2411
VspI ATTAAT 1 cut(s) 1670
XagI CCTNNNNNAGG 1 cut(s) 1080
XapI RAATTY 6 cut(s) 937, 961, 1878, 1888, 1958, 2387
XbaI TCTAGA 1 cut(s) 2494
XceI RCATGY 3 cut(s) 1305, 1581, 2270
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmaJI CCTAGG 1 cut(s) 2063
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 5 cut(s) 644, 2064, 2121, 2490, 2495
ZraI GACGTC 1 cut(s) 1483
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.