Rmu_sc0020102.1_g000004

Staphylococcal nuclease domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020102.1
OG0018417
Physical Location & Seq
Forward (+)
22741 .. 25368
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020102.1_g000004.1.cds

Sequence Viewer

Length: 2628 bp
atggcatcacaatggtataggggcagagtcaaggctgttccttcaggcgattcccttgtcatccaggcccttcagtcagacaagaccggaatcctacaggaaagaattatcactttgttatcagttcgtgctcccaaactggccggtaaagatcccagtactgatgagccttttgcatgggacagcagagagtatctgaggaagctctgcatcggaaaggaggtggtgttcagcattgagcagggcgtcggctctgttttccttgaaaacaataatgttgcagcccttgttgttcaaaaagggtgggcagctgtcacgtcaccgagcacaactccttacaattgtgagctgcagtttcacatggatcaagcctttaataaaggtcttggtcgctggagcacggttccaggtgcggcggaggcatccattaggactctacctccccaactcaacttagagaccatgaagactctgttggctgcaaacaagggcaagcccatagaagctattgtcgagcaggttgatgagggcagtattgttcaggcttacttgcttccggagtttcagtttgtccgtgtcattgttgcaggaactcaggcgccctacattggacctaatgcttatcctttcgcactagaagccaagtttttcacggagtctcgtgtgttgaatagagatgtgcatattgttctggaaggtgttgataagttcagccgtctgattggctctgtgtattaccccgatggagactccgaaaaggacttgggcctggagcttgtggagaatggctatgctaaatatgtaccatggagcgcgaaagtgatggatgaagatgcaagccggcggctcaagactgcagagcttgaagctaagaaaaacaagctggggatttggacacactttgtacccccagctacaaattccaagccaattcatgaccagaatttcacaggaatagttgtggaggttgtaagtggggaatgtgtcattgtggctaatgattctggagagcggagagtcaatctttcgagtatttggtgtccgaagatgggaaatcctcgtagaggtgatgagcctgctgcatatgcacgtgaagccaaggagttgctgaggacacgccttattggacgccaagtgaatgttcaaatggagtattcaaggaaggtcggccaagcagatgcagattcttccagagtaatagatttcggatcagtctttattaaggctgagggggaagataaaagcaaccaggctgaggaaaacgttgctgagcttgtggttgcatgtggcttcggcactgttatcaaacaccgagattttgaggagagatcaagatactatgatgtccttcttgctgccgaatcaggtgctatttctggaaagaaaggactacattgttccaaggaggcccccattgtgcacatgactgacttgacaatggcaacggccaagaaagcaagagacgtcttgccatccttgcagcgaagtaggagagtttctgcagttgtggaatatgtcctcagcggtcatcgttttaaactatacattcccaacgagacatgcagcatttccttctctctctccggtctgagatgtcccagtcgcgatgagccctattcggatgaagcaattgcagtaatgaggcaaagattaatgcagagagatgttgagattgaagtggagactgttgatagaaacggaactttcttgggaactctatgggagtcggggaccaatgtgtcaattgccctcgttgaagctggccttgccaaatatattccctttggtggcagtgacaaggtccaagatggccaccttcttgtacgagctatgaaaaatgcaaaaacgaagaagctgaaaatttgggaaaattttgaaggaaaacccaacaaacagaaagaactgcaggtagtgattacagaagttttggacagaggtaaattttatgtgcagacagttggggacgagaagagtgcctctattcagcaacagcttacttctttgaaccttgaagaagctccttctggaagtgacgcttttaatcctagggagggtgacattgtccttgctcagtttagtgcagataattcttggaaccgagctaggattgtcaatgcgcgaaaagacaagtttgaagtgttctacattgactatggtaatcaagaggttgttccttacagtcagttacggcctcttaactcttctgctgcctctgcacctggtcttgctcagctatgcagccttgcacatgttactgtcccaagcttggaggaggagtttggtcaagaagcaggagagtatttgagtgagtgcacattggacaaagagttcacggccgtgattgagggaagggatatttccggaggaaaatttgctaaaggccagggaaccggaccagctcttatggtaactctcttcgcggtagataatgctgacagtataaatgctgccatgcttcagaatggacttgctagtctagatcaccagaagaggtcttggaagtctcgtaccttgagagagttccaggaaaaggcacaaaccttccgccaggggatgtggcatgattattacgagtctgatcatcatgagaacagagctcctcctgcaagaaaggctgctggcaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000932 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009266 GO:0009408 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010371 GO:0010372 GO:0010467 GO:0010468 GO:0010494 GO:0010565 GO:0010605 GO:0010629 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016070 GO:0016071 GO:0016101 GO:0016102 GO:0016114 GO:0016787 GO:0016788 GO:0019216 GO:0019222 GO:0019439 GO:0019747 GO:0019752 GO:0030054 GO:0030312 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0043455 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046394 GO:0046483 GO:0046686 GO:0046700 GO:0046885 GO:0046886 GO:0046889 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060255 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090305 GO:0090351 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05169 ko05203 map05169 map05203
Pfam Domains
Protein Families
SNase TUDOR

Protein Analysis

875

Amino Acids

96.91

Weight (kDa)

6.17

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018417)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g14720
rosa_chinensis RchiOBHm_Chr6g0309151
rosa_laevigata RLG00000010586
rosa_multiflora Rmu_sc0020102.1_g000004
rosa_rugosa Rorug06G0370100
rosa_samantha Rh6AG482900 Rh6BG492000 Rh6CG496700 Rh6DG483600
rosa_wichuraiana Rw6G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1738, 2472
AatII GACGTC 1 cut(s) 1467
Acc36I ACCTGC 2 cut(s) 508, 1897
AccB1I GGYRCC 1 cut(s) 598
AccBSI CCGCTC 1 cut(s) 1012
AccII CGCG 4 cut(s) 815, 1605, 2119, 2420
AccIII TCCGGA 2 cut(s) 556, 2360
AciI CCGC 7 cut(s) 413, 416, 844, 1012, 1524, 2420, 2545
AclI AACGTT 1 cut(s) 1263
AclWI GGATC 3 cut(s) 146, 372, 1216
AcoI YGGCCR 5 cut(s) 141, 1168, 1446, 1810, 2334
AcsI RAATTY 6 cut(s) 919, 943, 1860, 1870, 1940, 2369
AcuI CTGAAG 3 cut(s) 27, 56, 2441
AcvI CACGTG 1 cut(s) 1091
AcyI GRCGYC 4 cut(s) 246, 599, 1129, 1464
AfaI GTAC 5 cut(s) 160, 804, 906, 1824, 2509
AfiI CCNNNNNNNGG 8 cut(s) 608, 1049, 1064, 1255, 1787, 2051, 2266, 2530
AflIII ACRYGT 1 cut(s) 2248
AjiI CACGTC 1 cut(s) 318
AjnI CCWGG 8 cut(s) 63, 406, 768, 1248, 2218, 2382, 2523, 2547
AjuI GAANNNNNNNTTGG 4 cut(s) 458, 490, 1125, 1157
AleI CACNNNNGTG 1 cut(s) 2336
AloI GAACNNNNNNTCC 4 cut(s) 212, 244, 2312, 2344
Alw21I GWGCWC 6 cut(s) 133, 329, 401, 1422, 2315, 2599
Alw26I GTCTC 7 cut(s) 452, 663, 741, 1455, 1550, 1676, 2508
Alw44I GTGCAC 2 cut(s) 1418, 2311
AlwI GGATC 3 cut(s) 146, 372, 1216
Aor13HI TCCGGA 2 cut(s) 556, 2360
ApaLI GTGCAC 2 cut(s) 1418, 2311
ApoI RAATTY 6 cut(s) 919, 943, 1860, 1870, 1940, 2369
AseI ATTAAT 1 cut(s) 1652
AspA2I CCTAGG 1 cut(s) 2045
AspLEI GCGC 3 cut(s) 601, 815, 2119
AspS9I GGNCC 7 cut(s) 67, 611, 766, 1408, 1731, 1801, 2393
AsuHPI GGTGA 4 cut(s) 312, 1079, 2066, 2474
AvaII GGWCC 4 cut(s) 611, 1731, 1801, 2393
AvrII CCTAGG 1 cut(s) 2045
BaeGI GKGCMC 2 cut(s) 1422, 2315
BalI TGGCCA 1 cut(s) 1812
BanI GGYRCC 1 cut(s) 598
BanII GRGCYC 2 cut(s) 1614, 2599
BauI CACGAG 1 cut(s) 660
BbrPI CACGTG 1 cut(s) 1091
BbsI GAAGAC 1 cut(s) 473
Bbv12I GWGCWC 6 cut(s) 133, 329, 401, 1422, 2315, 2599
BbvCI CCTCAGC 4 cut(s) 1109, 1227, 1254, 1520
BccI CCATC 5 cut(s) 737, 817, 1042, 1480, 1802
BceAI ACGGC 5 cut(s) 699, 1461, 2204, 2321, 2349
BcgI CGANNNNNNTGC 4 cut(s) 1349, 1383, 1955, 1989
BciT130I CCWGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
BclI TGATCA 1 cut(s) 2578
BcoDI GTCTC 7 cut(s) 452, 663, 741, 1455, 1550, 1676, 2508
BfaI CTAG 5 cut(s) 635, 2046, 2103, 2472, 2477
BfmI CTRYAG 5 cut(s) 95, 350, 855, 1500, 1904
BfoI RGCGCY 1 cut(s) 602
BfuAI ACCTGC 2 cut(s) 508, 1897
BlnI CCTAGG 1 cut(s) 2045
BlpI GCTNAGC 2 cut(s) 1269, 2229
BmcAI AGTACT 1 cut(s) 160
Bme1390I CCNGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
Bme18I GGWCC 4 cut(s) 611, 1731, 1801, 2393
BmgBI CACGTC 1 cut(s) 318
BmgT120I GGNCC 7 cut(s) 67, 611, 766, 1408, 1731, 1801, 2393
BmiI GGNNCC 6 cut(s) 405, 600, 1410, 1732, 2096, 2389
BmrFI CCNGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
BmrI ACTGGG 2 cut(s) 150, 1593
BmsI GCATC 5 cut(s) 14, 219, 431, 823, 1168
BmuI ACTGGG 2 cut(s) 150, 1593
BpiI GAAGAC 1 cut(s) 473
BplI GAGNNNNNCTC 2 cut(s) 316, 348
BpmI CTGGAG 3 cut(s) 415, 791, 1026
Bpu10I CCTNAGC 4 cut(s) 1109, 1227, 1254, 1520
Bpu1102I GCTNAGC 2 cut(s) 1269, 2229
BpuEI CTTGAG 2 cut(s) 833, 2533
BsaAI YACGTR 1 cut(s) 1091
BsaHI GRCGYC 4 cut(s) 246, 599, 1129, 1464
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 6 cut(s) 806, 1098, 1401, 2045, 2383, 2548
BsaWI WCCGGW 5 cut(s) 86, 556, 1583, 2360, 2390
BsaXI ACNNNNNCTCC 8 cut(s) 212, 242, 424, 454, 551, 581, 2261, 2291
Bsc4I CCNNNNNNNGG 8 cut(s) 608, 1049, 1064, 1255, 1787, 2051, 2266, 2530
Bse118I RCCGGY 2 cut(s) 143, 840
Bse1I ACTGG 3 cut(s) 144, 156, 1599
BseAI TCCGGA 2 cut(s) 556, 2360
BseBI CCWGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
BseDI CCNNGG 6 cut(s) 806, 1098, 1401, 2045, 2383, 2548
BseGI GGATG 6 cut(s) 60, 422, 832, 1472, 1627, 2559
BseLI CCNNNNNNNGG 8 cut(s) 608, 1049, 1064, 1255, 1787, 2051, 2266, 2530
BseNI ACTGG 3 cut(s) 144, 156, 1599
BseRI GAGGAG 4 cut(s) 1337, 2285, 2288, 2589
BseSI GKGCMC 2 cut(s) 1422, 2315
BseX3I CGGCCG 1 cut(s) 2334
BseYI CCCAGC 2 cut(s) 883, 910
BsgI GTGCAG 3 cut(s) 1970, 2100, 2199
Bsh1236I CGCG 4 cut(s) 815, 1605, 2119, 2420
Bsh1285I CGRYCG 1 cut(s) 2337
BshNI GGYRCC 1 cut(s) 598
BsiEI CGRYCG 1 cut(s) 2337
BsiHKAI GWGCWC 6 cut(s) 133, 329, 401, 1422, 2315, 2599
BsiSI CCGG 7 cut(s) 87, 144, 557, 841, 1584, 2361, 2391
BslFI GGGAC 5 cut(s) 194, 1581, 1744, 1976, 2243
BslI CCNNNNNNNGG 8 cut(s) 608, 1049, 1064, 1255, 1787, 2051, 2266, 2530
BsmAI GTCTC 7 cut(s) 452, 663, 741, 1455, 1550, 1676, 2508
BsmBI CGTCTC 1 cut(s) 1455
BsmFI GGGAC 5 cut(s) 194, 1581, 1744, 1976, 2243
Bso31I GGTCTC 1 cut(s) 452
Bsp1286I GDGCHC 7 cut(s) 133, 329, 401, 1422, 1614, 2315, 2599
Bsp13I TCCGGA 2 cut(s) 556, 2360
Bsp143I GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
Bsp1720I GCTNAGC 2 cut(s) 1269, 2229
Bsp19I CCATGG 1 cut(s) 806
Bsp68I TCGCGA 1 cut(s) 1605
BspACI CCGC 7 cut(s) 413, 416, 844, 1012, 1524, 2420, 2545
BspEI TCCGGA 2 cut(s) 556, 2360
BspFNI CGCG 4 cut(s) 815, 1605, 2119, 2420
BspHI TCATGA 2 cut(s) 934, 2584
BspLI GGNNCC 6 cut(s) 405, 600, 1410, 1732, 2096, 2389
BspMAI CTGCAG 4 cut(s) 354, 859, 1504, 1908
BspMI ACCTGC 2 cut(s) 508, 1897
BspPI GGATC 3 cut(s) 146, 372, 1216
BspT107I GGYRCC 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 452
BsrBI CCGCTC 1 cut(s) 1012
BsrFI RCCGGY 2 cut(s) 143, 840
BsrI ACTGG 3 cut(s) 144, 156, 1599
BssAI RCCGGY 2 cut(s) 143, 840
BssECI CCNNGG 6 cut(s) 806, 1098, 1401, 2045, 2383, 2548
BssMI GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
BssNI GRCGYC 4 cut(s) 246, 599, 1129, 1464
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 4 cut(s) 806, 1098, 1401, 2045
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
Bst4CI ACNGT 7 cut(s) 403, 1300, 1687, 1957, 2180, 2257, 2438
Bst6I CTCTTC 4 cut(s) 1964, 2206, 2420, 2483
BstACI GRCGYC 4 cut(s) 246, 599, 1129, 1464
BstBAI YACGTR 1 cut(s) 1091
BstC8I GCNNGC 6 cut(s) 494, 838, 842, 1077, 1762, 2620
BstDSI CCRYGG 1 cut(s) 806
BstENI CCTNNNNNAGG 1 cut(s) 1062
BstF5I GGATG 6 cut(s) 60, 422, 832, 1472, 1627, 2559
BstFNI CGCG 4 cut(s) 815, 1605, 2119, 2420
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 3 cut(s) 601, 815, 2119
BstKTI GATC 6 cut(s) 154, 367, 1211, 1331, 2482, 2581
BstMAI GTCTC 7 cut(s) 452, 663, 741, 1455, 1550, 1676, 2508
BstMBI GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
BstMCI CGRYCG 1 cut(s) 2337
BstNI CCWGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
BstNSI RCATGY 3 cut(s) 1287, 1563, 2252
BstSCI CCNGG 8 cut(s) 63, 406, 768, 1248, 2218, 2382, 2523, 2547
BstSFI CTRYAG 5 cut(s) 95, 350, 855, 1500, 1904
BstSLI GKGCMC 2 cut(s) 1422, 2315
BstUI CGCG 4 cut(s) 815, 1605, 2119, 2420
BstV2I GAAGAC 1 cut(s) 473
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BstZI CGGCCG 1 cut(s) 2334
BtgI CCRYGG 1 cut(s) 806
BtgZI GCGATG 1 cut(s) 1620
BtrI CACGTC 1 cut(s) 318
BtsCI GGATG 6 cut(s) 60, 422, 832, 1472, 1627, 2559
BtsI GCAGTG 1 cut(s) 1798
BtsIMutI CAGTG 2 cut(s) 1296, 1798
BtuMI TCGCGA 1 cut(s) 1605
BveI ACCTGC 2 cut(s) 508, 1897
Cac8I GCNNGC 6 cut(s) 494, 838, 842, 1077, 1762, 2620
CciI TCATGA 2 cut(s) 934, 2584
CfoI GCGC 3 cut(s) 601, 815, 2119
Cfr10I RCCGGY 2 cut(s) 143, 840
Cfr13I GGNCC 7 cut(s) 67, 611, 766, 1408, 1731, 1801, 2393
CseI GACGC 3 cut(s) 235, 1137, 2042
CsiI ACCWGGT 1 cut(s) 2218
Csp6I GTAC 5 cut(s) 159, 803, 905, 1823, 2508
CspCI CAANNNNNGTGG 2 cut(s) 284, 319
CviQI GTAC 5 cut(s) 159, 803, 905, 1823, 2508
DinI GGCGCC 1 cut(s) 600
DpnI GATC 6 cut(s) 153, 366, 1210, 1330, 2481, 2580
DpnII GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
DraI TTTAAA 1 cut(s) 1537
DrdI GACNNNNNNGTC 2 cut(s) 1738, 2472
DseDI GACNNNNNNGTC 2 cut(s) 1738, 2472
EaeI YGGCCR 5 cut(s) 141, 1168, 1446, 1810, 2334
EagI CGGCCG 1 cut(s) 2334
Eam1104I CTCTTC 4 cut(s) 1964, 2206, 2420, 2483
EarI CTCTTC 4 cut(s) 1964, 2206, 2420, 2483
EciI GGCGGA 2 cut(s) 431, 2534
Ecl136II GAGCTC 1 cut(s) 2597
EclXI CGGCCG 1 cut(s) 2334
Eco130I CCWWGG 4 cut(s) 806, 1098, 1401, 2045
Eco24I GRGCYC 2 cut(s) 1614, 2599
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 4 cut(s) 611, 1731, 1801, 2393
Eco52I CGGCCG 1 cut(s) 2334
Eco53kI GAGCTC 1 cut(s) 2597
Eco57I CTGAAG 3 cut(s) 27, 56, 2441
Eco72I CACGTG 1 cut(s) 1091
EcoICRI GAGCTC 1 cut(s) 2597
EcoNI CCTNNNNNAGG 1 cut(s) 1062
EcoO109I RGGNCCY 2 cut(s) 67, 1408
EcoRII CCWGG 8 cut(s) 63, 406, 768, 1248, 2218, 2382, 2523, 2547
EcoT14I CCWWGG 4 cut(s) 806, 1098, 1401, 2045
EcoT38I GRGCYC 2 cut(s) 1614, 2599
EgeI GGCGCC 1 cut(s) 600
EheI GGCGCC 1 cut(s) 600
ErhI CCWWGG 4 cut(s) 806, 1098, 1401, 2045
Esp3I CGTCTC 1 cut(s) 1455
FalI AAGNNNNNCTT 4 cut(s) 1749, 1781, 2020, 2052
FaqI GGGAC 5 cut(s) 194, 1581, 1744, 1976, 2243
FauNDI CATATG 1 cut(s) 1084
FbaI TGATCA 1 cut(s) 2578
FokI GGATG 6 cut(s) 47, 409, 839, 1459, 1634, 2566
FriOI GRGCYC 2 cut(s) 1614, 2599
FspBI CTAG 5 cut(s) 635, 2046, 2103, 2472, 2477
GlaI GCGC 3 cut(s) 600, 814, 2118
GsaI CCCAGC 2 cut(s) 887, 914
GsuI CTGGAG 3 cut(s) 415, 791, 1026
HaeII RGCGCY 1 cut(s) 602
HapII CCGG 7 cut(s) 87, 144, 557, 841, 1584, 2361, 2391
HgaI GACGC 3 cut(s) 235, 1137, 2042
HhaI GCGC 3 cut(s) 601, 815, 2119
Hin1I GRCGYC 4 cut(s) 246, 599, 1129, 1464
Hin6I GCGC 3 cut(s) 599, 813, 2117
HinP1I GCGC 3 cut(s) 599, 813, 2117
HindIII AAGCTT 1 cut(s) 2263
HpaII CCGG 7 cut(s) 87, 144, 557, 841, 1584, 2361, 2391
HphI GGTGA 4 cut(s) 312, 1079, 2066, 2474
Hpy166II GTNNAC 3 cut(s) 1420, 2313, 2331
Hpy8I GTNNAC 3 cut(s) 1420, 2313, 2331
Hpy99I CGWCG 1 cut(s) 251
HpyCH4III ACNGT 7 cut(s) 403, 1300, 1687, 1957, 2180, 2257, 2438
HpyCH4IV ACGT 4 cut(s) 317, 1090, 1263, 1464
HpySE526I ACGT 4 cut(s) 317, 1090, 1263, 1464
Hsp92I GRCGYC 4 cut(s) 246, 599, 1129, 1464
HspAI GCGC 3 cut(s) 599, 813, 2117
KasI GGCGCC 1 cut(s) 598
Kpn2I TCCGGA 2 cut(s) 556, 2360
KroI GCCGGC 1 cut(s) 840
KroNI GCCGGC 1 cut(s) 842
Ksp22I TGATCA 1 cut(s) 2578
Kzo9I GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
LmnI GCTCC 6 cut(s) 136, 396, 772, 810, 2023, 2602
LweI GCATC 5 cut(s) 14, 219, 431, 823, 1168
MabI ACCWGGT 1 cut(s) 2218
MaeI CTAG 5 cut(s) 635, 2046, 2103, 2472, 2477
MaeII ACGT 4 cut(s) 317, 1090, 1263, 1464
MaeIII GTNAC 8 cut(s) 313, 318, 1793, 2030, 2054, 2184, 2251, 2407
MalI GATC 6 cut(s) 153, 366, 1210, 1330, 2481, 2580
MbiI CCGCTC 1 cut(s) 1012
MboI GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
MfeI CAATTG 3 cut(s) 340, 1629, 1743
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 7 cut(s) 133, 329, 401, 1422, 1614, 2315, 2599
MlsI TGGCCA 1 cut(s) 1812
MluNI TGGCCA 1 cut(s) 1812
Mly113I GGCGCC 1 cut(s) 599
MlyI GAGTC 8 cut(s) 36, 427, 463, 665, 743, 1026, 1733, 2582
Mox20I TGGCCA 1 cut(s) 1812
MroI TCCGGA 2 cut(s) 556, 2360
MroNI GCCGGC 1 cut(s) 840
MscI TGGCCA 1 cut(s) 1812
MseI TTAA 6 cut(s) 375, 1221, 1536, 1652, 2040, 2196
MslI CAYNNNNRTG 2 cut(s) 10, 2336
Msp20I TGGCCA 1 cut(s) 1812
MspA1I CMGCKG 2 cut(s) 311, 1524
MspI CCGG 7 cut(s) 87, 144, 557, 841, 1584, 2361, 2391
MspR9I CCNGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
MunI CAATTG 3 cut(s) 340, 1629, 1743
MvaI CCWGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
MvnI CGCG 4 cut(s) 815, 1605, 2119, 2420
NaeI GCCGGC 1 cut(s) 842
NarI GGCGCC 1 cut(s) 599
NcoI CCATGG 1 cut(s) 806
NdeI CATATG 1 cut(s) 1084
NdeII GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
NgoMIV GCCGGC 1 cut(s) 840
NlaIV GGNNCC 6 cut(s) 405, 600, 1410, 1732, 2096, 2389
NmuCI GTSAC 5 cut(s) 313, 318, 1793, 2030, 2054
NruI TCGCGA 1 cut(s) 1605
NspI RCATGY 3 cut(s) 1287, 1563, 2252
OliI CACNNNNGTG 1 cut(s) 2336
PagI TCATGA 2 cut(s) 934, 2584
PciI ACATGT 1 cut(s) 2248
PdiI GCCGGC 1 cut(s) 842
PfeI GAWTC 5 cut(s) 50, 90, 1001, 1184, 1361
PflFI GACNNNGTC 2 cut(s) 1799, 2060
PfoI TCCNGGA 1 cut(s) 2523
PleI GAGTC 8 cut(s) 35, 427, 463, 664, 743, 1025, 1732, 2581
PluTI GGCGCC 1 cut(s) 602
PmaCI CACGTG 1 cut(s) 1091
PmlI CACGTG 1 cut(s) 1091
PpsI GAGTC 8 cut(s) 35, 427, 463, 664, 743, 1025, 1732, 2581
Ppu21I YACGTR 1 cut(s) 1091
PscI ACATGT 1 cut(s) 2248
PshBI ATTAAT 1 cut(s) 1652
Psp124BI GAGCTC 1 cut(s) 2599
Psp1406I AACGTT 1 cut(s) 1263
Psp6I CCWGG 8 cut(s) 63, 406, 768, 1248, 2218, 2382, 2523, 2547
PspCI CACGTG 1 cut(s) 1091
PspFI CCCAGC 2 cut(s) 883, 910
PspGI CCWGG 8 cut(s) 63, 406, 768, 1248, 2218, 2382, 2523, 2547
PspN4I GGNNCC 6 cut(s) 405, 600, 1410, 1732, 2096, 2389
PspPI GGNCC 7 cut(s) 67, 611, 766, 1408, 1731, 1801, 2393
PstI CTGCAG 4 cut(s) 354, 859, 1504, 1908
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 2 cut(s) 1799, 2060
PvuII CAGCTG 1 cut(s) 311
RruI TCGCGA 1 cut(s) 1605
RsaI GTAC 5 cut(s) 160, 804, 906, 1824, 2509
RsaNI GTAC 5 cut(s) 159, 803, 905, 1823, 2508
RseI CAYNNNNRTG 2 cut(s) 10, 2336
SacI GAGCTC 1 cut(s) 2599
SaqAI TTAA 6 cut(s) 375, 1221, 1536, 1652, 2040, 2196
Sau3AI GATC 6 cut(s) 151, 364, 1208, 1328, 2479, 2578
Sau96I GGNCC 7 cut(s) 67, 611, 766, 1408, 1731, 1801, 2393
ScaI AGTACT 1 cut(s) 160
SchI GAGTC 8 cut(s) 36, 427, 463, 665, 743, 1026, 1733, 2582
ScrFI CCNGG 8 cut(s) 65, 408, 770, 1250, 2220, 2384, 2525, 2549
SduI GDGCHC 7 cut(s) 133, 329, 401, 1422, 1614, 2315, 2599
SexAI ACCWGGT 1 cut(s) 2218
SfaNI GCATC 5 cut(s) 14, 219, 431, 823, 1168
SfcI CTRYAG 5 cut(s) 95, 350, 855, 1500, 1904
SfoI GGCGCC 1 cut(s) 600
SinI GGWCC 4 cut(s) 611, 1731, 1801, 2393
SmiMI CAYNNNNRTG 2 cut(s) 10, 2336
SmlI CTYRAG 2 cut(s) 848, 2512
SmoI CTYRAG 2 cut(s) 848, 2512
SsiI CCGC 7 cut(s) 413, 416, 844, 1012, 1524, 2420, 2545
SspDI GGCGCC 1 cut(s) 598
SspMI CTAG 5 cut(s) 635, 2046, 2103, 2472, 2477
SstI GAGCTC 1 cut(s) 2599
StyD4I CCNGG 8 cut(s) 63, 406, 768, 1248, 2218, 2382, 2523, 2547
StyI CCWWGG 4 cut(s) 806, 1098, 1401, 2045
TaaI ACNGT 7 cut(s) 403, 1300, 1687, 1957, 2180, 2257, 2438
TaiI ACGT 4 cut(s) 320, 1093, 1266, 1467
TaqI TCGA 2 cut(s) 513, 1028
TatI WGTACW 1 cut(s) 158
TauI GCSGC 2 cut(s) 416, 847
TfiI GAWTC 5 cut(s) 50, 90, 1001, 1184, 1361
Tru1I TTAA 6 cut(s) 375, 1221, 1536, 1652, 2040, 2196
Tru9I TTAA 6 cut(s) 375, 1221, 1536, 1652, 2040, 2196
TscAI CASTG 2 cut(s) 1303, 1798
TseFI GTSAC 5 cut(s) 313, 318, 1793, 2030, 2054
Tsp45I GTSAC 5 cut(s) 313, 318, 1793, 2030, 2054
TspDTI ATGAA 5 cut(s) 479, 843, 923, 1638, 1847
TspGWI ACGGA 3 cut(s) 563, 668, 1713
TspRI CASTG 2 cut(s) 1303, 1798
Tth111I GACNNNGTC 2 cut(s) 1799, 2060
VneI GTGCAC 2 cut(s) 1418, 2311
VpaK11BI GGWCC 4 cut(s) 611, 1731, 1801, 2393
VspI ATTAAT 1 cut(s) 1652
XagI CCTNNNNNAGG 1 cut(s) 1062
XapI RAATTY 6 cut(s) 919, 943, 1860, 1870, 1940, 2369
XbaI TCTAGA 1 cut(s) 2476
XceI RCATGY 3 cut(s) 1287, 1563, 2252
XmaJI CCTAGG 1 cut(s) 2045
XspI CTAG 5 cut(s) 635, 2046, 2103, 2472, 2477
ZraI GACGTC 1 cut(s) 1465
ZrmI AGTACT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.