Rh6DG483600

Staphylococcal nuclease domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0018417
Physical Location & Seq
Forward (+)
64660743 .. 64663430
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG483600.1

Sequence Viewer

Length: 2688 bp
ATGGCATCACAATGGTATAGGGGCAGAGTCAAGGCTGTTCCTTCAGGCGATTCCCTTGTCATCCAGGCCCTTCAGTCAAACAAGACCAGAATCCCACCGGAAAAAAGTCAAAAAACTATTACTTTGTTATCAGTTCGTGCTCCCAAACTGGCCGGTGAAGATCGCAGTACTGATGAGCCTTTTGCATGGGACAGCAGAGAGTATCTGAGGAAGCTCTGCCTCGGAAAGGAGGTGGTTTTCAGCACTGATAACTTTGCAGAACCGCAGGGCGTCGGCTCTGTTTTCCTTGAAAACAATAATGTTGCAGCCCTTGTTGTTCAAAAAGGGTGGGCAGCTGTCACGTCACCGAGCACAACTCCTTACAATTGTGAGCTGCAGTTTCACATGGATCAAGCCTTTAATAAAGGTCTTGGTCGCTGGAGCACGGTTCCAGGTGCCGCGGAGGCATCCATTAGGACTCTACCTCCCCAACTCAACTTAGAGACCATGAAGACTCTGTTGGCTGCAAACAAGGGCAAGCCCATAGAAGCTATTGTCGAGGAGGTTGTTGATGGCAGTATTGTTCTGGCTCACTTGCTTCCGGAGTTTCAGTTTGTCCCAGTCATTGTTGCAGGAACTCAGGCGCCCTACATTGGACCTGATGCTTATCCTTTGATTCCTTTCGCACTAGAAGCTAAGTTTTTCACAGAGTCTCGTGTGTTGAATAGAGATGTGCATATTGTTCTGGAAGGTGTTGATGAGCTGGAAGGTGTTGATGAGTTCAGCAAGTTCAGCCGTCTGATTGGGTCTCTGTATTACCCGGATGGAGACTCCGAAAAGGACTTAGGGCTGGAGCTTGTGGAGAATGGTTATGCTAAATATGTACCATGGAGTGCGAAAGTGATGGGTGAAGCTGCAAGGCAGCGGCTCAAGACTGCAGAGTTTGAAGCTAAGAAAAACAAGCTGAGGATTTGGACACACTTTGTACCCCCAGCTACAAATTCCAAGCCAATTCATGACCAGAATTTCACGGGAACAGTTGTGGAGGTTGTAAGTGGGGAATGTGTCATTGTGGCTAATGATTCTGGAGAGCGGAGAGTCAATCTTTCGAGTATTTGGTGTCCGAAGATGGGAAATCCTCGTAGAGGTGATAAGCCAGCTGCATATGCACGTGAAGCCAAGGAGTTGCTGAGGACACGCCTTATTGGATGCCAAGTGAATGTTCAAATGGAGTATTCAAGGAAGGTAGGCCAAGCAGATGCAGATTCTTCCAGAGTAATAGATTTTGGATCAGTCTTTATTAAGGCTGAGGGGGAAGATAAAAGCAACCAGGCTGAGGAAAACGTTGCTGAGCTTGTGGTTGCATGTGGCTTCGGCAATGTTATCAAACACCGAGATTTTGAGGAGAGATCAAGATACTATGATGCCCTTCTTGCTGCCGAATCAGGCGCTATTGCTGGAAAGAAAGGACTACATTCTTCCAAGGAGGCCCCCGTTGTGCACATGACAGACTTGACAATGATAACGGCCAAGAAAGCAAGAGACGTCTTGCCATCCTTGCAGCGAAGTAGGAGAGTTTCTGCAGTTGTGGAATATGTCCTCAGCGGTCATCGTTTTAAACTATACATTCCCAAGGAGACATGCAGCATTTCCTTCTCTCTCTCCGGTGTGAGATGTCCCGGTCGCGATGAGCCCTATTCGGATGAAGCAATTGCAGTAATGAGGCGAAGATTAATGCAGAGAGATGTTGAGATTGAAGTGGAGACTGTTGATAGAAACGGAACTTTCTTGGGAACTCTATGGGAGTCAGGGACCAATGTGTCAATTGCCCTCGTTGAAGCTGGCCTTGCCAAATATATTCCCTTTGTTGGCAGTGACAAGATCCAAGATGGCCACCTTCTTGAACGAGCTATGAAAAATGCAAAAACGAAGAAGCTGAAAATTTGGGAAAATTTTGAAGGAAAACCCAACAAACAGAAAGAATTAAAGGTAGTGATTACAGAAGTTTTGGACAGAGGTAAATTTTATGTGCAGACAGTTGGGGACGAGAAGAGTGCCTCTATTCAGCAACAGCTTGCTTCTTTGAACCTTGAAGAATCTCCTTCTGCAAGTGATGCTTTTAATCCTAGGGAGGGTAACATTGTCCTTGCTCAGTTTAGTGCAGATAATTCTTGGAACCGAGCTAGGATTGTCAATGCGCGAAAAGACAAGTTTGAAGTGTTCTACATTGACTATGGTAATCAAGAGGTTGTCCCTTACAGTCAGTTACGGCCTCTTAACTCTTCTGCTGCCTCTGCACCTGGTCTTGCTCAGCTATGCAGCCTTGCACATGTTACTGTCCCAAGCTTGGAGGAGGAGTTTGGTCAAGAAGCAGGAGAGTATTTGAGTGAGTGCACATTGGACAAAGAGTTCACGGCCGTGATTGAGGGAAGGGATATTTCCGGAGGAAAATTTGCTAAAGGCCAGGGAACCGGACCAGCTCTTATGGTAACTCTCTTCGCAGTAGATAATGCTGACAGTATAAATGCTGCCATGCTTCAGAATGGACTTGCTAGTCTAGATAACCAGAAGAGGTCTTGGAAGTCTCGTACCTTGAGAGAGTTCCAGGAGAAAGCACAAACCTTCCGCCAGGGGATGTGGCATGATTATTACGAGTCTGATTATCATGAGAACAGAGCTCCTCCTGCAAGAAAGGCTGCTGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000932 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009266 GO:0009408 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010371 GO:0010372 GO:0010467 GO:0010468 GO:0010494 GO:0010565 GO:0010605 GO:0010629 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016070 GO:0016071 GO:0016101 GO:0016102 GO:0016114 GO:0016787 GO:0016788 GO:0019216 GO:0019222 GO:0019439 GO:0019747 GO:0019752 GO:0030054 GO:0030312 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0043455 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046394 GO:0046483 GO:0046686 GO:0046700 GO:0046885 GO:0046886 GO:0046889 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060255 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090305 GO:0090351 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05169 ko05203 map05169 map05203
Pfam Domains
Protein Families
SNase TUDOR

Protein Analysis

895

Amino Acids

99.15

Weight (kDa)

6.33

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNase PF00565 39 - 141 1.6e-06 Staphylococcal nuclease homologue
SNase PF00565 217 - 319 3e-10 Staphylococcal nuclease homologue
SNase PF00565 545 - 644 4.2e-15 Staphylococcal nuclease homologue
TUDOR PF00567 651 - 770 5.3e-23 Tudor domain
SNase PF00565 776 - 875 1.3e-07 Staphylococcal nuclease homologue
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018417)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g14720
rosa_chinensis RchiOBHm_Chr6g0309151
rosa_laevigata RLG00000010586
rosa_multiflora Rmu_sc0020102.1_g000004
rosa_rugosa Rorug06G0370100
rosa_samantha Rh6AG482900 Rh6BG492000 Rh6CG496700 Rh6DG483600
rosa_wichuraiana Rw6G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1798, 2532
AatII GACGTC 1 cut(s) 1527
AccB1I GGYRCC 2 cut(s) 434, 622
AccBSI CCGCTC 1 cut(s) 1072
AccII CGCG 3 cut(s) 440, 1665, 2179
AccIII TCCGGA 2 cut(s) 580, 2420
AciI CCGC 7 cut(s) 263, 438, 440, 904, 1072, 1584, 2605
AclI AACGTT 1 cut(s) 1323
AclWI GGATC 3 cut(s) 396, 1276, 1855
AcoI YGGCCR 4 cut(s) 150, 1506, 1870, 2394
AcsI RAATTY 6 cut(s) 979, 1003, 1920, 1930, 2000, 2429
AcuI CTGAAG 3 cut(s) 27, 56, 2501
AcvI CACGTG 1 cut(s) 1151
AcyI GRCGYC 3 cut(s) 270, 623, 1524
AfaI GTAC 4 cut(s) 169, 864, 966, 2569
AfiI CCNNNNNNNGG 8 cut(s) 226, 632, 1109, 1124, 1315, 1847, 2111, 2326
AflIII ACRYGT 1 cut(s) 2308
AjiI CACGTC 1 cut(s) 342
AjnI CCWGG 7 cut(s) 63, 430, 1308, 2278, 2442, 2583, 2607
AjuI GAANNNNNNNTTGG 4 cut(s) 482, 514, 1185, 1217
AleI CACNNNNGTG 1 cut(s) 2396
AloI GAACNNNNNNTCC 2 cut(s) 2372, 2404
Alw21I GWGCWC 6 cut(s) 142, 353, 425, 1482, 2375, 2659
Alw26I GTCTC 8 cut(s) 476, 696, 792, 801, 1515, 1610, 1736, 2568
Alw44I GTGCAC 2 cut(s) 1478, 2371
AlwI GGATC 3 cut(s) 396, 1276, 1855
Aor13HI TCCGGA 2 cut(s) 580, 2420
ApaLI GTGCAC 2 cut(s) 1478, 2371
ApoI RAATTY 6 cut(s) 979, 1003, 1920, 1930, 2000, 2429
AseI ATTAAT 1 cut(s) 1712
AspA2I CCTAGG 1 cut(s) 2105
AspLEI GCGC 3 cut(s) 625, 1430, 2179
AspS9I GGNCC 5 cut(s) 67, 635, 1468, 1791, 2453
AsuC2I CCSGG 2 cut(s) 800, 1659
AsuHPI GGTGA 4 cut(s) 167, 336, 899, 1139
AvaII GGWCC 3 cut(s) 635, 1791, 2453
AvrII CCTAGG 1 cut(s) 2105
BaeGI GKGCMC 2 cut(s) 1482, 2375
BalI TGGCCA 1 cut(s) 1872
BanI GGYRCC 2 cut(s) 434, 622
BanII GRGCYC 2 cut(s) 1674, 2659
BauI CACGAG 1 cut(s) 693
BbrPI CACGTG 1 cut(s) 1151
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 6 cut(s) 142, 353, 425, 1482, 2375, 2659
BbvCI CCTCAGC 5 cut(s) 944, 1169, 1287, 1314, 1580
BccI CCATC 6 cut(s) 545, 797, 877, 1102, 1540, 1862
BceAI ACGGC 5 cut(s) 759, 1521, 2264, 2381, 2409
BcgI CGANNNNNNTGC 2 cut(s) 2015, 2049
BciT130I CCWGG 7 cut(s) 65, 432, 1310, 2280, 2444, 2585, 2609
BcnI CCSGG 2 cut(s) 800, 1659
BcoDI GTCTC 8 cut(s) 476, 696, 792, 801, 1515, 1610, 1736, 2568
BfaI CTAG 5 cut(s) 668, 2106, 2163, 2532, 2537
BfmI CTRYAG 3 cut(s) 374, 915, 1560
BfoI RGCGCY 2 cut(s) 626, 1431
BglI GCCNNNNNGGC 1 cut(s) 443
BlnI CCTAGG 1 cut(s) 2105
BlpI GCTNAGC 2 cut(s) 1329, 2289
BmcAI AGTACT 1 cut(s) 169
Bme1390I CCNGG 9 cut(s) 65, 432, 800, 1310, 1659, 2280, 2444, 2585, 2609
Bme18I GGWCC 3 cut(s) 635, 1791, 2453
BmgBI CACGTC 1 cut(s) 342
BmgT120I GGNCC 5 cut(s) 67, 635, 1468, 1791, 2453
BmiI GGNNCC 7 cut(s) 429, 436, 624, 1470, 1792, 2156, 2449
BmrFI CCNGG 9 cut(s) 65, 432, 800, 1310, 1659, 2280, 2444, 2585, 2609
BmrI ACTGGG 1 cut(s) 593
BmsI GCATC 7 cut(s) 14, 455, 631, 1178, 1228, 1393, 2083
BmuI ACTGGG 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 497
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 3 cut(s) 439, 851, 1086
Bpu10I CCTNAGC 5 cut(s) 944, 1169, 1287, 1314, 1580
Bpu1102I GCTNAGC 2 cut(s) 1329, 2289
BpuEI CTTGAG 2 cut(s) 893, 2593
BpuMI CCSGG 2 cut(s) 800, 1659
BsaAI YACGTR 1 cut(s) 1151
BsaBI GATNNNNATC 1 cut(s) 645
BsaHI GRCGYC 3 cut(s) 270, 623, 1524
BsaI GGTCTC 2 cut(s) 476, 792
BsaJI CCNNGG 9 cut(s) 220, 438, 866, 1158, 1461, 1611, 2105, 2443, 2608
BsaWI WCCGGW 5 cut(s) 97, 580, 1643, 2420, 2450
BsaXI ACNNNNNCTCC 6 cut(s) 448, 478, 575, 605, 2321, 2351
Bsc4I CCNNNNNNNGG 8 cut(s) 226, 632, 1109, 1124, 1315, 1847, 2111, 2326
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 153, 599
Bse3DI GCAATG 1 cut(s) 1363
Bse8I GATNNNNATC 1 cut(s) 645
BseAI TCCGGA 2 cut(s) 580, 2420
BseBI CCWGG 7 cut(s) 65, 432, 1310, 2280, 2444, 2585, 2609
BseDI CCNNGG 9 cut(s) 220, 438, 866, 1158, 1461, 1611, 2105, 2443, 2608
BseGI GGATG 7 cut(s) 60, 446, 808, 1193, 1532, 1687, 2619
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 8 cut(s) 226, 632, 1109, 1124, 1315, 1847, 2111, 2326
BseMI GCAATG 1 cut(s) 1363
BseNI ACTGG 2 cut(s) 153, 599
BseRI GAGGAG 5 cut(s) 554, 1397, 2345, 2348, 2649
BseSI GKGCMC 2 cut(s) 1482, 2375
BseX3I CGGCCG 1 cut(s) 2394
BseYI CCCAGC 1 cut(s) 970
BsgI GTGCAG 3 cut(s) 2030, 2160, 2259
Bsh1236I CGCG 3 cut(s) 440, 1665, 2179
Bsh1285I CGRYCG 2 cut(s) 1663, 2397
BshNI GGYRCC 2 cut(s) 434, 622
BsiEI CGRYCG 2 cut(s) 1663, 2397
BsiHKAI GWGCWC 6 cut(s) 142, 353, 425, 1482, 2375, 2659
BsiSI CCGG 8 cut(s) 98, 153, 581, 800, 1644, 1659, 2421, 2451
BslFI GGGAC 7 cut(s) 203, 581, 1641, 1804, 2036, 2216, 2303
BslI CCNNNNNNNGG 8 cut(s) 226, 632, 1109, 1124, 1315, 1847, 2111, 2326
BsmAI GTCTC 8 cut(s) 476, 696, 792, 801, 1515, 1610, 1736, 2568
BsmBI CGTCTC 1 cut(s) 1515
BsmFI GGGAC 7 cut(s) 203, 581, 1641, 1804, 2036, 2216, 2303
Bso31I GGTCTC 2 cut(s) 476, 792
Bsp1286I GDGCHC 7 cut(s) 142, 353, 425, 1482, 1674, 2375, 2659
Bsp13I TCCGGA 2 cut(s) 580, 2420
Bsp143I GATC 5 cut(s) 160, 388, 1268, 1388, 1860
Bsp1720I GCTNAGC 2 cut(s) 1329, 2289
Bsp19I CCATGG 1 cut(s) 866
Bsp68I TCGCGA 1 cut(s) 1665
BspACI CCGC 7 cut(s) 263, 438, 440, 904, 1072, 1584, 2605
BspEI TCCGGA 2 cut(s) 580, 2420
BspFNI CGCG 3 cut(s) 440, 1665, 2179
BspHI TCATGA 2 cut(s) 994, 2644
BspLI GGNNCC 7 cut(s) 429, 436, 624, 1470, 1792, 2156, 2449
BspMAI CTGCAG 3 cut(s) 378, 919, 1564
BspPI GGATC 3 cut(s) 396, 1276, 1855
BspT107I GGYRCC 2 cut(s) 434, 622
BspTNI GGTCTC 2 cut(s) 476, 792
BsrBI CCGCTC 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 1363
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 153, 599
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 9 cut(s) 220, 438, 866, 1158, 1461, 1611, 2105, 2443, 2608
BssMI GATC 5 cut(s) 160, 388, 1268, 1388, 1860
BssNI GRCGYC 3 cut(s) 270, 623, 1524
BssSI CACGAG 1 cut(s) 693
BssT1I CCWWGG 5 cut(s) 866, 1158, 1461, 1611, 2105
Bst2BI CACGAG 1 cut(s) 693
Bst2UI CCWGG 7 cut(s) 65, 432, 1310, 2280, 2444, 2585, 2609
Bst4CI ACNGT 7 cut(s) 427, 1018, 1747, 2017, 2240, 2317, 2498
Bst6I CTCTTC 4 cut(s) 2024, 2266, 2480, 2543
BstACI GRCGYC 3 cut(s) 270, 623, 1524
BstAPI GCANNNNNTGC 1 cut(s) 2093
BstBAI YACGTR 1 cut(s) 1151
BstC8I GCNNGC 5 cut(s) 518, 1137, 1822, 2055, 2680
BstDSI CCRYGG 2 cut(s) 438, 866
BstENI CCTNNNNNAGG 2 cut(s) 224, 1122
BstF5I GGATG 7 cut(s) 60, 446, 808, 1193, 1532, 1687, 2619
BstFNI CGCG 3 cut(s) 440, 1665, 2179
BstH2I RGCGCY 2 cut(s) 626, 1431
BstHHI GCGC 3 cut(s) 625, 1430, 2179
BstKTI GATC 5 cut(s) 163, 391, 1271, 1391, 1863
BstMAI GTCTC 8 cut(s) 476, 696, 792, 801, 1515, 1610, 1736, 2568
BstMBI GATC 5 cut(s) 160, 388, 1268, 1388, 1860
BstMCI CGRYCG 2 cut(s) 1663, 2397
BstNI CCWGG 7 cut(s) 65, 432, 1310, 2280, 2444, 2585, 2609
BstNSI RCATGY 3 cut(s) 1347, 1623, 2312
BstSCI CCNGG 9 cut(s) 63, 430, 798, 1308, 1657, 2278, 2442, 2583, 2607
BstSFI CTRYAG 3 cut(s) 374, 915, 1560
BstSLI GKGCMC 2 cut(s) 1482, 2375
BstUI CGCG 3 cut(s) 440, 1665, 2179
BstV2I GAAGAC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 1860
BstYI RGATCY 1 cut(s) 1860
BstZI CGGCCG 1 cut(s) 2394
BtgI CCRYGG 2 cut(s) 438, 866
BtgZI GCGATG 1 cut(s) 1680
BtrI CACGTC 1 cut(s) 342
BtsCI GGATG 7 cut(s) 60, 446, 808, 1193, 1532, 1687, 2619
BtsI GCAGTG 1 cut(s) 1858
BtsIMutI CAGTG 2 cut(s) 243, 1858
BtuMI TCGCGA 1 cut(s) 1665
Cac8I GCNNGC 5 cut(s) 518, 1137, 1822, 2055, 2680
CciI TCATGA 2 cut(s) 994, 2644
CfoI GCGC 3 cut(s) 625, 1430, 2179
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 5 cut(s) 67, 635, 1468, 1791, 2453
Cfr42I CCGCGG 1 cut(s) 441
CseI GACGC 1 cut(s) 259
CsiI ACCWGGT 1 cut(s) 2278
Csp6I GTAC 4 cut(s) 168, 863, 965, 2568
CspCI CAANNNNNGTGG 2 cut(s) 308, 343
CviQI GTAC 4 cut(s) 168, 863, 965, 2568
DinI GGCGCC 1 cut(s) 624
DpnI GATC 5 cut(s) 162, 390, 1270, 1390, 1862
DpnII GATC 5 cut(s) 160, 388, 1268, 1388, 1860
DraI TTTAAA 1 cut(s) 1597
DrdI GACNNNNNNGTC 2 cut(s) 1798, 2532
DseDI GACNNNNNNGTC 2 cut(s) 1798, 2532
EaeI YGGCCR 4 cut(s) 150, 1506, 1870, 2394
EagI CGGCCG 1 cut(s) 2394
Eam1104I CTCTTC 4 cut(s) 2024, 2266, 2480, 2543
EarI CTCTTC 4 cut(s) 2024, 2266, 2480, 2543
EciI GGCGGA 1 cut(s) 2594
Ecl136II GAGCTC 1 cut(s) 2657
EclXI CGGCCG 1 cut(s) 2394
Eco130I CCWWGG 5 cut(s) 866, 1158, 1461, 1611, 2105
Eco24I GRGCYC 2 cut(s) 1674, 2659
Eco31I GGTCTC 2 cut(s) 476, 792
Eco47I GGWCC 3 cut(s) 635, 1791, 2453
Eco52I CGGCCG 1 cut(s) 2394
Eco53kI GAGCTC 1 cut(s) 2657
Eco57I CTGAAG 3 cut(s) 27, 56, 2501
Eco72I CACGTG 1 cut(s) 1151
EcoICRI GAGCTC 1 cut(s) 2657
EcoNI CCTNNNNNAGG 2 cut(s) 224, 1122
EcoO109I RGGNCCY 2 cut(s) 67, 1468
EcoRII CCWGG 7 cut(s) 63, 430, 1308, 2278, 2442, 2583, 2607
EcoT14I CCWWGG 5 cut(s) 866, 1158, 1461, 1611, 2105
EcoT38I GRGCYC 2 cut(s) 1674, 2659
EgeI GGCGCC 1 cut(s) 624
EheI GGCGCC 1 cut(s) 624
ErhI CCWWGG 5 cut(s) 866, 1158, 1461, 1611, 2105
Esp3I CGTCTC 1 cut(s) 1515
FalI AAGNNNNNCTT 4 cut(s) 1809, 1841, 2080, 2112
FaqI GGGAC 7 cut(s) 203, 581, 1641, 1804, 2036, 2216, 2303
FauNDI CATATG 1 cut(s) 1144
FokI GGATG 7 cut(s) 47, 433, 815, 1200, 1519, 1694, 2626
FriOI GRGCYC 2 cut(s) 1674, 2659
FspBI CTAG 5 cut(s) 668, 2106, 2163, 2532, 2537
GlaI GCGC 3 cut(s) 624, 1429, 2178
GsaI CCCAGC 1 cut(s) 974
GsuI CTGGAG 3 cut(s) 439, 851, 1086
HaeII RGCGCY 2 cut(s) 626, 1431
HapII CCGG 8 cut(s) 98, 153, 581, 800, 1644, 1659, 2421, 2451
HgaI GACGC 1 cut(s) 259
HhaI GCGC 3 cut(s) 625, 1430, 2179
Hin1I GRCGYC 3 cut(s) 270, 623, 1524
Hin6I GCGC 3 cut(s) 623, 1428, 2177
HinP1I GCGC 3 cut(s) 623, 1428, 2177
HindIII AAGCTT 1 cut(s) 2323
HpaII CCGG 8 cut(s) 98, 153, 581, 800, 1644, 1659, 2421, 2451
HphI GGTGA 4 cut(s) 167, 336, 899, 1139
Hpy166II GTNNAC 3 cut(s) 1480, 2373, 2391
Hpy188I TCNGA 8 cut(s) 207, 224, 780, 814, 1104, 1681, 2520, 2638
Hpy8I GTNNAC 3 cut(s) 1480, 2373, 2391
Hpy99I CGWCG 1 cut(s) 275
HpyCH4III ACNGT 7 cut(s) 427, 1018, 1747, 2017, 2240, 2317, 2498
HpyCH4IV ACGT 4 cut(s) 341, 1150, 1323, 1524
HpySE526I ACGT 4 cut(s) 341, 1150, 1323, 1524
Hsp92I GRCGYC 3 cut(s) 270, 623, 1524
HspAI GCGC 3 cut(s) 623, 1428, 2177
KasI GGCGCC 1 cut(s) 622
Kpn2I TCCGGA 2 cut(s) 580, 2420
KspI CCGCGG 1 cut(s) 441
Kzo9I GATC 5 cut(s) 160, 388, 1268, 1388, 1860
LmnI GCTCC 4 cut(s) 145, 420, 832, 2662
LweI GCATC 7 cut(s) 14, 455, 631, 1178, 1228, 1393, 2083
MabI ACCWGGT 1 cut(s) 2278
MaeI CTAG 5 cut(s) 668, 2106, 2163, 2532, 2537
MaeII ACGT 4 cut(s) 341, 1150, 1323, 1524
MaeIII GTNAC 7 cut(s) 337, 342, 1853, 2114, 2244, 2311, 2467
MalI GATC 5 cut(s) 162, 390, 1270, 1390, 1862
MbiI CCGCTC 1 cut(s) 1072
MboI GATC 5 cut(s) 160, 388, 1268, 1388, 1860
MfeI CAATTG 3 cut(s) 364, 1689, 1803
MflI RGATCY 1 cut(s) 1860
MhlI GDGCHC 7 cut(s) 142, 353, 425, 1482, 1674, 2375, 2659
MlsI TGGCCA 1 cut(s) 1872
MluNI TGGCCA 1 cut(s) 1872
Mly113I GGCGCC 1 cut(s) 623
MlyI GAGTC 8 cut(s) 36, 451, 487, 698, 803, 1086, 1793, 2642
Mox20I TGGCCA 1 cut(s) 1872
MroI TCCGGA 2 cut(s) 580, 2420
MscI TGGCCA 1 cut(s) 1872
MseI TTAA 7 cut(s) 399, 1281, 1596, 1712, 1964, 2100, 2256
MslI CAYNNNNRTG 2 cut(s) 10, 2396
Msp20I TGGCCA 1 cut(s) 1872
MspA1I CMGCKG 5 cut(s) 335, 440, 904, 1139, 1584
MspI CCGG 8 cut(s) 98, 153, 581, 800, 1644, 1659, 2421, 2451
MspR9I CCNGG 9 cut(s) 65, 432, 800, 1310, 1659, 2280, 2444, 2585, 2609
MunI CAATTG 3 cut(s) 364, 1689, 1803
MvaI CCWGG 7 cut(s) 65, 432, 1310, 2280, 2444, 2585, 2609
MvnI CGCG 3 cut(s) 440, 1665, 2179
NarI GGCGCC 1 cut(s) 623
NciI CCSGG 2 cut(s) 800, 1659
NcoI CCATGG 1 cut(s) 866
NdeI CATATG 1 cut(s) 1144
NdeII GATC 5 cut(s) 160, 388, 1268, 1388, 1860
NlaIV GGNNCC 7 cut(s) 429, 436, 624, 1470, 1792, 2156, 2449
NmuCI GTSAC 3 cut(s) 337, 342, 1853
NruI TCGCGA 1 cut(s) 1665
NspI RCATGY 3 cut(s) 1347, 1623, 2312
OliI CACNNNNGTG 1 cut(s) 2396
PagI TCATGA 2 cut(s) 994, 2644
PciI ACATGT 1 cut(s) 2308
PfeI GAWTC 7 cut(s) 50, 90, 655, 1061, 1244, 1421, 2075
PfoI TCCNGGA 1 cut(s) 2583
PleI GAGTC 8 cut(s) 35, 451, 487, 697, 803, 1085, 1792, 2641
PluTI GGCGCC 1 cut(s) 626
PmaCI CACGTG 1 cut(s) 1151
PmlI CACGTG 1 cut(s) 1151
PpsI GAGTC 8 cut(s) 35, 451, 487, 697, 803, 1085, 1792, 2641
Ppu21I YACGTR 1 cut(s) 1151
PscI ACATGT 1 cut(s) 2308
PshBI ATTAAT 1 cut(s) 1712
Psp124BI GAGCTC 1 cut(s) 2659
Psp1406I AACGTT 1 cut(s) 1323
Psp6I CCWGG 7 cut(s) 63, 430, 1308, 2278, 2442, 2583, 2607
PspCI CACGTG 1 cut(s) 1151
PspFI CCCAGC 1 cut(s) 970
PspGI CCWGG 7 cut(s) 63, 430, 1308, 2278, 2442, 2583, 2607
PspN4I GGNNCC 7 cut(s) 429, 436, 624, 1470, 1792, 2156, 2449
PspPI GGNCC 5 cut(s) 67, 635, 1468, 1791, 2453
PstI CTGCAG 3 cut(s) 378, 919, 1564
PsuI RGATCY 1 cut(s) 1860
PvuII CAGCTG 2 cut(s) 335, 1139
RruI TCGCGA 1 cut(s) 1665
RsaI GTAC 4 cut(s) 169, 864, 966, 2569
RsaNI GTAC 4 cut(s) 168, 863, 965, 2568
RseI CAYNNNNRTG 2 cut(s) 10, 2396
SacI GAGCTC 1 cut(s) 2659
SacII CCGCGG 1 cut(s) 441
SaqAI TTAA 7 cut(s) 399, 1281, 1596, 1712, 1964, 2100, 2256
Sau3AI GATC 5 cut(s) 160, 388, 1268, 1388, 1860
Sau96I GGNCC 5 cut(s) 67, 635, 1468, 1791, 2453
ScaI AGTACT 1 cut(s) 169
SchI GAGTC 8 cut(s) 36, 451, 487, 698, 803, 1086, 1793, 2642
ScrFI CCNGG 9 cut(s) 65, 432, 800, 1310, 1659, 2280, 2444, 2585, 2609
SduI GDGCHC 7 cut(s) 142, 353, 425, 1482, 1674, 2375, 2659
SexAI ACCWGGT 1 cut(s) 2278
SfaNI GCATC 7 cut(s) 14, 455, 631, 1178, 1228, 1393, 2083
SfcI CTRYAG 3 cut(s) 374, 915, 1560
SfoI GGCGCC 1 cut(s) 624
Sfr303I CCGCGG 1 cut(s) 441
SgrBI CCGCGG 1 cut(s) 441
SinI GGWCC 3 cut(s) 635, 1791, 2453
SmiMI CAYNNNNRTG 2 cut(s) 10, 2396
SmlI CTYRAG 2 cut(s) 908, 2572
SmoI CTYRAG 2 cut(s) 908, 2572
SsiI CCGC 7 cut(s) 263, 438, 440, 904, 1072, 1584, 2605
SspDI GGCGCC 1 cut(s) 622
SspMI CTAG 5 cut(s) 668, 2106, 2163, 2532, 2537
SstI GAGCTC 1 cut(s) 2659
StyD4I CCNGG 9 cut(s) 63, 430, 798, 1308, 1657, 2278, 2442, 2583, 2607
StyI CCWWGG 5 cut(s) 866, 1158, 1461, 1611, 2105
TaaI ACNGT 7 cut(s) 427, 1018, 1747, 2017, 2240, 2317, 2498
TaiI ACGT 4 cut(s) 344, 1153, 1326, 1527
TaqI TCGA 2 cut(s) 537, 1088
TatI WGTACW 1 cut(s) 167
TauI GCSGC 2 cut(s) 440, 907
TfiI GAWTC 7 cut(s) 50, 90, 655, 1061, 1244, 1421, 2075
Tru1I TTAA 7 cut(s) 399, 1281, 1596, 1712, 1964, 2100, 2256
Tru9I TTAA 7 cut(s) 399, 1281, 1596, 1712, 1964, 2100, 2256
TscAI CASTG 2 cut(s) 250, 1858
TseFI GTSAC 3 cut(s) 337, 342, 1853
Tsp45I GTSAC 3 cut(s) 337, 342, 1853
TspDTI ATGAA 4 cut(s) 503, 983, 1698, 1907
TspGWI ACGGA 1 cut(s) 1773
TspRI CASTG 2 cut(s) 250, 1858
VneI GTGCAC 2 cut(s) 1478, 2371
VpaK11BI GGWCC 3 cut(s) 635, 1791, 2453
VspI ATTAAT 1 cut(s) 1712
XagI CCTNNNNNAGG 2 cut(s) 224, 1122
XapI RAATTY 6 cut(s) 979, 1003, 1920, 1930, 2000, 2429
XbaI TCTAGA 1 cut(s) 2536
XceI RCATGY 3 cut(s) 1347, 1623, 2312
XmaJI CCTAGG 1 cut(s) 2105
XspI CTAG 5 cut(s) 668, 2106, 2163, 2532, 2537
ZraI GACGTC 1 cut(s) 1525
ZrmI AGTACT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.