Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0011221

Physical Location & Seq

Genomic Coordinates Reverse (-)

7307456 .. 7311474

Gene Structure 4019 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011170

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGGGGAAATCTAACTCCTGCTTCAAAATTATCACATGCGGCAACGATTCAGAGGACAAAGACGATCTCGAAGCTTCTGAGAGCAAGGGTTCAGGTGACAAACGTGGCTGGAGTTTCCGTAAGAGGTCTGCACGGCATCGAGTGCTAAGCAACACTGTGATCACCGAAACCCCCACTTCAGAGAATAAGGAGAGCCCAGAATCTGCCCCTCTTACTTTCCAATCACCAGCTAGCACGACTGTTCCAGAGAAATTATCTGTAATACAATGCACCGATGAGAAACCACAGTTGGCGACTCCTGAGAACCCAAAAGTTTCTACAACTGAAGATGCCTCCGAAGATCAAAGCAAGGTTGAGGACAAACTGGACGAGTCTGCTGTTGTTGTTGTCCAGACTGCTGTCAGAGGATTGTTGGCTCAAAGATCATTGCTGGAGCTGAAAAATGTAGTCAAGTTGCAAGCTGCTGTCCGTGGCCATTTGGTGCGTAGGCATGCTGTTGGAACTTTGCGTTGTGTTCAAGCCATTGTCAAAATGCAAGCTCTTGTTCGTGCTCGTCAATCTCTTCAGAAGGAAAACCATAGTTCAAAAACCTCGAAGAAGGCAAATGTAACCTACATTTCCATTGAAAAGCTACTTAGCAATAGCTTTGCCCGCCAGCTCTTAGAATCAACACCGAAGAACCAACCTATTCATGTTAAGTGTGATCCTTCTAAGCCTGATTCTGCTTGGAAATGGTTGGAAATGTGGATGTCAGTCTCAACGAAGGATACTCCACATTTAAACGAGGCAGTAGCAGTGGTAGAGCAACAGGAAACAGAAAAGGAAGAAATTCCCGAGTCTCCACGTGAATCTAAAACTCAATATTGTGAGATGGATGAATCAAATTCCAGCATAAGGGAGATTGCGCTTTCTGAAAGTGAAGAAAGTCTGATTACTTATGATGCCGACAACTTGAACTTTCAGGCAAGCTATTCTACCTCTCATACAGTGAGAGACAATAATGAGCAGCCTCAGTTGGAGAACACCACTACATCTGCTGTGAAAGAGTCCCCAGTAGAAATAAATCTATCAGATGCAGATTGTAACATGGAGTTCAAAGCTTTATCTGGGAAACCTTCAAAATTTGCAGAGCTGACTTCAACAACCAATTCAGCTAGGTCGATCAGTTCATCTCCTCAAGATGTTTCTGGAGTTGAACCACAAAGGGACACATTCTCATCTGAGGTGGATACTGAAGTCAGAGCAAAGGAGCTAAGTGTAGCAGAGAATCCAATCCCCCATGGATCACAAGTTCAAGTGGGTGGTTCTGAGTGTGGCACCGAAATCTCTATTTCTTCCACTCTTGATTCGCCTGATAGATCTGATATTGGAGCCATGGAGCGTGACCATGACCATGATGCGAAAGTTTCGGAGGAAAGGATTTGCAGTCCAAAAAATACAAGAGACATTGATGTTGAAACTACGGATTTCCTGGAAACCCCAGTCTCCAACTTATGTAACGCTGTTGCAGAACCAGAGAAAATTGGTGCTGTCAATGGTGAAACCATAAATTCTGAGGCTGATCAAGAACCAAGAGATAGCAGCAGCAGTAGTTCTATTCCTCATTTCATGCAAGCCACAGAGTCTGCTAGAGCAAAGCTTCAAGCAACTGCCTCTCCCAGATCGAGTCCAGATGTGCAAGACAGGGAGATTTACATCAAGAAGAGACATTCCTTACCTGGTGTGAATGGAAGGCAGGGATCTCCTCGAGTCCAGCGGTCCATGTCTCAGGCTCAGACACAGCAGGGTGCAAAGGGCACCGAGAGAAAATGGCAGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005875 GO:0005886 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009719 GO:0009725 GO:0009737 GO:0009941 GO:0010033 GO:0015630 GO:0016020 GO:0031967 GO:0031975 GO:0032991 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4005 IQ

Protein Analysis

607

Amino Acids

66.66

Weight (kDa)

5.26

Isoelectric Point (pI)

54.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4005	PF13178	520 - 594	6.4e-09	Protein of unknown function (DUF4005)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19870 AT1G19870
fragaria_vesca	FvH4_2g38820 FvH4_2g38820 FvH4_2g38820
malus_domestica	MD08G1015800.v1.1 MD15G1014800.v1.1
prunus_persica	Prupe.1G369000_v2.0.a1
pyrus_communis	pycom08g01380
rosa_chinensis	RchiOBHm_Chr6g0302451
rosa_laevigata	RLG00000011170
rosa_multiflora	Rmu_sc0011021.1_g000014
rosa_roxburghii	Rroxscaffold_7G00165760
rosa_rugosa	Rorug06G0314400 Rorug06G0314400
rosa_samantha	Rh6AG427400 Rh6BG475300 Rh6CG439500 Rh6DG426500
rosa_wichuraiana	Rw6G036970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1316, 1796
AciI	CCGC	3 cut(s)	39, 652, 1756
AclWI	GGATC	3 cut(s)	698, 1291, 1747
AcoI	YGGCCR	1 cut(s)	472
AcsI	RAATTY	4 cut(s)	828, 883, 1121, 1549
AcuI	CTGAAG	4 cut(s)	162, 345, 548, 1254
AcvI	CACGTG	1 cut(s)	845
AfiI	CCNNNNNNNGG	1 cut(s)	894
AjnI	CCWGG	2 cut(s)	1470, 1717
AjuI	GAANNNNNNNTTGG	2 cut(s)	272, 304
AloI	GAACNNNNNNTCC	2 cut(s)	1275, 1307
Alw21I	GWGCWC	1 cut(s)	553
Alw26I	GTCTC	7 cut(s)	760, 843, 987, 1437, 1489, 1699, 1770
AlwI	GGATC	3 cut(s)	698, 1291, 1747
AlwNI	CAGNNNCTG	2 cut(s)	203, 1625
Ama87I	CYCGRG	2 cut(s)	833, 1746
AoxI	GGCC	1 cut(s)	472
ApeKI	GCWGC	4 cut(s)	461, 1006, 1581, 1584
ApoI	RAATTY	4 cut(s)	828, 883, 1121, 1549
Asp700I	GAANNNNTTC	1 cut(s)	828
AspLEI	GCGC	1 cut(s)	907
AspS9I	GGNCC	1 cut(s)	1758
AsuHPI	GGTGA	4 cut(s)	107, 154, 216, 1550
AsuNHI	GCTAGC	1 cut(s)	230
AvaI	CYCGRG	2 cut(s)	833, 1746
AvaII	GGWCC	1 cut(s)	1758
BaeGI	GKGCMC	1 cut(s)	1799
BaeI	ACNNNNGTAYC	2 cut(s)	1221, 1254
BalI	TGGCCA	1 cut(s)	474
BanI	GGYRCC	2 cut(s)	1316, 1796
BanII	GRGCYC	1 cut(s)	197
BbrPI	CACGTG	1 cut(s)	845
Bbv12I	GWGCWC	1 cut(s)	553
BbvI	GCAGC	4 cut(s)	448, 1018, 1593, 1596
BccI	CCATC	1 cut(s)	865
BceAI	ACGGC	1 cut(s)	149
BciT130I	CCWGG	2 cut(s)	1472, 1719
BciVI	GTATCC	2 cut(s)	760, 1222
BclI	TGATCA	2 cut(s)	159, 1561
BcoDI	GTCTC	7 cut(s)	760, 843, 987, 1437, 1489, 1699, 1770
BfaI	CTAG	3 cut(s)	231, 1155, 1629
BfuI	GTATCC	2 cut(s)	760, 1222
BglII	AGATCT	1 cut(s)	1358
BisI	GCNGC	5 cut(s)	40, 462, 1007, 1582, 1585
BlpI	GCTNAGC	1 cut(s)	146
BlsI	GCNGC	5 cut(s)	41, 463, 1008, 1583, 1586
Bme1390I	CCNGG	2 cut(s)	1472, 1719
Bme18I	GGWCC	1 cut(s)	1758
BmeT110I	CYCGRG	2 cut(s)	833, 1746
BmgT120I	GGNCC	1 cut(s)	1758
BmiI	GGNNCC	3 cut(s)	1318, 1372, 1798
BmrFI	CCNGG	2 cut(s)	1472, 1719
BmrI	ACTGGG	2 cut(s)	1046, 1475
BmsI	GCATC	5 cut(s)	145, 319, 931, 1063, 1387
BmtI	GCTAGC	1 cut(s)	234
BmuI	ACTGGG	2 cut(s)	1046, 1475
BoxI	GACNNNNGTC	1 cut(s)	398
BpmI	CTGGAG	3 cut(s)	130, 452, 1209
Bpu1102I	GCTNAGC	1 cut(s)	146
BpuEI	CTTGAG	1 cut(s)	1161
BsaAI	YACGTR	1 cut(s)	845
BsaBI	GATNNNNATC	1 cut(s)	1694
BsaJI	CCNNGG	3 cut(s)	469, 1279, 1374
BsaXI	ACNNNNNCTCC	4 cut(s)	103, 133, 1639, 1669
Bsc4I	CCNNNNNNNGG	1 cut(s)	894
Bse1I	ACTGG	3 cut(s)	369, 1052, 1481
Bse3DI	GCAATG	1 cut(s)	425
Bse8I	GATNNNNATC	1 cut(s)	1694
BseBI	CCWGG	2 cut(s)	1472, 1719
BseDI	CCNNGG	3 cut(s)	469, 1279, 1374
BseGI	GGATG	2 cut(s)	753, 880
BseJI	GATNNNNATC	1 cut(s)	1694
BseLI	CCNNNNNNNGG	1 cut(s)	894
BseMI	GCAATG	1 cut(s)	425
BseMII	CTCAG	8 cut(s)	69, 291, 1025, 1212, 1299, 1545, 1781, 1787
BseNI	ACTGG	3 cut(s)	369, 1052, 1481
BseRI	GAGGAG	2 cut(s)	1164, 1734
BseSI	GKGCMC	1 cut(s)	1799
BseXI	GCAGC	4 cut(s)	448, 1018, 1593, 1596
BsgI	GTGCAG	1 cut(s)	114
BshFI	GGCC	1 cut(s)	474
BshNI	GGYRCC	2 cut(s)	1316, 1796
BsiHKAI	GWGCWC	1 cut(s)	553
BsiHKCI	CYCGRG	2 cut(s)	833, 1746
BslFI	GGGAC	2 cut(s)	1033, 1220
BslI	CCNNNNNNNGG	1 cut(s)	894
BsmAI	GTCTC	7 cut(s)	760, 843, 987, 1437, 1489, 1699, 1770
BsmFI	GGGAC	2 cut(s)	1033, 1220
BsnI	GGCC	1 cut(s)	474
BsoBI	CYCGRG	2 cut(s)	833, 1746
Bsp1286I	GDGCHC	3 cut(s)	197, 553, 1799
Bsp1720I	GCTNAGC	1 cut(s)	146
Bsp19I	CCATGG	2 cut(s)	1279, 1374
BspACI	CCGC	3 cut(s)	39, 652, 1756
BspANI	GGCC	1 cut(s)	474
BspCNI	CTCAG	8 cut(s)	70, 292, 1024, 1213, 1300, 1546, 1780, 1786
BspLI	GGNNCC	3 cut(s)	1318, 1372, 1798
BspOI	GCTAGC	1 cut(s)	234
BspPI	GGATC	3 cut(s)	698, 1291, 1747
BspT107I	GGYRCC	2 cut(s)	1316, 1796
BsrDI	GCAATG	1 cut(s)	425
BsrI	ACTGG	3 cut(s)	369, 1052, 1481
BssECI	CCNNGG	3 cut(s)	469, 1279, 1374
BssT1I	CCWWGG	2 cut(s)	1279, 1374
Bst2UI	CCWGG	2 cut(s)	1472, 1719
Bst4CI	ACNGT	4 cut(s)	157, 241, 288, 988
Bst6I	CTCTTC	2 cut(s)	567, 1697
BstAPI	GCANNNNNTGC	1 cut(s)	142
BstBAI	YACGTR	1 cut(s)	845
BstC8I	GCNNGC	8 cut(s)	232, 459, 492, 537, 652, 656, 967, 1614
BstDSI	CCRYGG	3 cut(s)	469, 1279, 1374
BstF5I	GGATG	2 cut(s)	753, 880
BstHHI	GCGC	1 cut(s)	907
BstMAI	GTCTC	7 cut(s)	760, 843, 987, 1437, 1489, 1699, 1770
BstMWI	GCNNNNNNNGC	2 cut(s)	142, 651
BstNI	CCWGG	2 cut(s)	1472, 1719
BstNSI	RCATGY	2 cut(s)	39, 494
BstPAI	GACNNNNGTC	1 cut(s)	398
BstSCI	CCNGG	2 cut(s)	1470, 1717
BstSLI	GKGCMC	1 cut(s)	1799
BstV1I	GCAGC	4 cut(s)	448, 1018, 1593, 1596
BstX2I	RGATCY	2 cut(s)	1358, 1739
BstYI	RGATCY	2 cut(s)	1358, 1739
BsuI	GTATCC	2 cut(s)	760, 1222
BsuRI	GGCC	1 cut(s)	474
BtgI	CCRYGG	3 cut(s)	469, 1279, 1374
BtsCI	GGATG	2 cut(s)	753, 880
BtsI	GCAGTG	1 cut(s)	801
BtsIMutI	CAGTG	3 cut(s)	153, 801, 993
Cac8I	GCNNGC	8 cut(s)	232, 459, 492, 537, 652, 656, 967, 1614
CaiI	CAGNNNCTG	2 cut(s)	203, 1625
CfoI	GCGC	1 cut(s)	907
Cfr13I	GGNCC	1 cut(s)	1758
CsiI	ACCWGGT	1 cut(s)	1717
DraI	TTTAAA	1 cut(s)	780
EaeI	YGGCCR	1 cut(s)	472
Eam1104I	CTCTTC	2 cut(s)	567, 1697
EarI	CTCTTC	2 cut(s)	567, 1697
Eco130I	CCWWGG	2 cut(s)	1279, 1374
Eco24I	GRGCYC	1 cut(s)	197
Eco47I	GGWCC	1 cut(s)	1758
Eco57I	CTGAAG	4 cut(s)	162, 345, 548, 1254
Eco72I	CACGTG	1 cut(s)	845
Eco88I	CYCGRG	2 cut(s)	833, 1746
EcoRII	CCWGG	2 cut(s)	1470, 1717
EcoT14I	CCWWGG	2 cut(s)	1279, 1374
EcoT38I	GRGCYC	1 cut(s)	197
ErhI	CCWWGG	2 cut(s)	1279, 1374
FaqI	GGGAC	2 cut(s)	1033, 1220
FauI	CCCGC	1 cut(s)	659
FbaI	TGATCA	2 cut(s)	159, 1561
Fnu4HI	GCNGC	5 cut(s)	40, 462, 1007, 1582, 1585
FokI	GGATG	2 cut(s)	760, 887
FriOI	GRGCYC	1 cut(s)	197
Fsp4HI	GCNGC	5 cut(s)	40, 462, 1007, 1582, 1585
FspBI	CTAG	3 cut(s)	231, 1155, 1629
GlaI	GCGC	1 cut(s)	906
GluI	GCNGC	5 cut(s)	40, 462, 1007, 1582, 1585
GsuI	CTGGAG	3 cut(s)	130, 452, 1209
HaeIII	GGCC	1 cut(s)	474
HhaI	GCGC	1 cut(s)	907
Hin6I	GCGC	1 cut(s)	905
HinP1I	GCGC	1 cut(s)	905
HindIII	AAGCTT	3 cut(s)	72, 1098, 1637
HphI	GGTGA	4 cut(s)	107, 154, 216, 1550
HpyAV	CCTTC	6 cut(s)	562, 592, 717, 757, 1125, 1725
HpyCH4III	ACNGT	4 cut(s)	157, 241, 288, 988
HpyCH4IV	ACGT	2 cut(s)	103, 844
HpyF10VI	GCNNNNNNNGC	2 cut(s)	142, 651
HpySE526I	ACGT	2 cut(s)	103, 844
HspAI	GCGC	1 cut(s)	905
Ksp22I	TGATCA	2 cut(s)	159, 1561
LmnI	GCTCC	4 cut(s)	433, 1249, 1370, 1378
Lsp1109I	GCAGC	4 cut(s)	448, 1018, 1593, 1596
LweI	GCATC	5 cut(s)	145, 319, 931, 1063, 1387
MabI	ACCWGGT	1 cut(s)	1717
MaeI	CTAG	3 cut(s)	231, 1155, 1629
MaeII	ACGT	2 cut(s)	103, 844
MaeIII	GTNAC	5 cut(s)	95, 607, 1082, 1382, 1496
MboII	GAAGA	9 cut(s)	338, 350, 554, 607, 688, 836, 932, 1326, 1714
MflI	RGATCY	2 cut(s)	1358, 1739
MhlI	GDGCHC	3 cut(s)	197, 553, 1799
MlsI	TGGCCA	1 cut(s)	474
MluCI	AATT	8 cut(s)	27, 251, 828, 883, 1121, 1147, 1521, 1549
MluNI	TGGCCA	1 cut(s)	474
MlyI	GAGTC	7 cut(s)	289, 380, 845, 1055, 1631, 1675, 1758
MmeI	TCCRAC	4 cut(s)	478, 717, 996, 1512
Mox20I	TGGCCA	1 cut(s)	474
MroXI	GAANNNNTTC	1 cut(s)	828
MscI	TGGCCA	1 cut(s)	474
MseI	TTAA	2 cut(s)	696, 779
MslI	CAYNNNNRTG	1 cut(s)	1392
Msp20I	TGGCCA	1 cut(s)	474
MspA1I	CMGCKG	1 cut(s)	1756
MspR9I	CCNGG	2 cut(s)	1472, 1719
MvaI	CCWGG	2 cut(s)	1472, 1719
MwoI	GCNNNNNNNGC	2 cut(s)	142, 651
NcoI	CCATGG	2 cut(s)	1279, 1374
NheI	GCTAGC	1 cut(s)	230
NlaIV	GGNNCC	3 cut(s)	1318, 1372, 1798
NmuCI	GTSAC	2 cut(s)	95, 1382
NspI	RCATGY	2 cut(s)	39, 494
PaeI	GCATGC	1 cut(s)	494
PaeR7I	CTCGAG	1 cut(s)	1746
PdmI	GAANNNNTTC	1 cut(s)	828
PfeI	GAWTC	8 cut(s)	47, 200, 665, 719, 848, 878, 1267, 1346
PfoI	TCCNGGA	1 cut(s)	1470
PkrI	GCNGC	5 cut(s)	41, 463, 1008, 1583, 1586
PleI	GAGTC	7 cut(s)	289, 379, 844, 1054, 1630, 1674, 1757
PmaCI	CACGTG	1 cut(s)	845
PmlI	CACGTG	1 cut(s)	845
PpsI	GAGTC	7 cut(s)	289, 379, 844, 1054, 1630, 1674, 1757
Ppu21I	YACGTR	1 cut(s)	845
PshAI	GACNNNNGTC	1 cut(s)	398
Psp6I	CCWGG	2 cut(s)	1470, 1717
PspCI	CACGTG	1 cut(s)	845
PspGI	CCWGG	2 cut(s)	1470, 1717
PspN4I	GGNNCC	3 cut(s)	1318, 1372, 1798
PspPI	GGNCC	1 cut(s)	1758
PspXI	VCTCGAGB	1 cut(s)	1746
PstNI	CAGNNNCTG	2 cut(s)	203, 1625
PsuI	RGATCY	2 cut(s)	1358, 1739
RseI	CAYNNNNRTG	1 cut(s)	1392
SaqAI	TTAA	2 cut(s)	696, 779
SatI	GCNGC	5 cut(s)	40, 462, 1007, 1582, 1585
Sau96I	GGNCC	1 cut(s)	1758
SchI	GAGTC	7 cut(s)	289, 380, 845, 1055, 1631, 1675, 1758
ScrFI	CCNGG	2 cut(s)	1472, 1719
SduI	GDGCHC	3 cut(s)	197, 553, 1799
SexAI	ACCWGGT	1 cut(s)	1717
SfaNI	GCATC	5 cut(s)	145, 319, 931, 1063, 1387
Sfr274I	CTCGAG	1 cut(s)	1746
SinI	GGWCC	1 cut(s)	1758
SlaI	CTCGAG	1 cut(s)	1746
SmiMI	CAYNNNNRTG	1 cut(s)	1392
SmlI	CTYRAG	2 cut(s)	1176, 1746
SmoI	CTYRAG	2 cut(s)	1176, 1746
SphI	GCATGC	1 cut(s)	494
Sse9I	AATT	8 cut(s)	27, 251, 828, 883, 1121, 1147, 1521, 1549
SsiI	CCGC	3 cut(s)	39, 652, 1756
SspI	AATATT	1 cut(s)	863
SspMI	CTAG	3 cut(s)	231, 1155, 1629
StyD4I	CCNGG	2 cut(s)	1470, 1717
StyI	CCWWGG	2 cut(s)	1279, 1374
TaaI	ACNGT	4 cut(s)	157, 241, 288, 988
TaiI	ACGT	2 cut(s)	106, 847
TaqI	TCGA	6 cut(s)	69, 139, 593, 1160, 1664, 1747
TasI	AATT	8 cut(s)	27, 251, 828, 883, 1121, 1147, 1521, 1549
TauI	GCSGC	1 cut(s)	42
TfiI	GAWTC	8 cut(s)	47, 200, 665, 719, 848, 878, 1267, 1346
Tru1I	TTAA	2 cut(s)	696, 779
Tru9I	TTAA	2 cut(s)	696, 779
TscAI	CASTG	3 cut(s)	160, 801, 993
TseFI	GTSAC	2 cut(s)	95, 1382
TseI	GCWGC	4 cut(s)	461, 1006, 1581, 1584
Tsp45I	GTSAC	2 cut(s)	95, 1382
TspDTI	ATGAA	4 cut(s)	680, 891, 1158, 1597
TspGWI	ACGGA	3 cut(s)	107, 458, 1478
TspRI	CASTG	3 cut(s)	160, 801, 993
VpaK11BI	GGWCC	1 cut(s)	1758
XapI	RAATTY	4 cut(s)	828, 883, 1121, 1549
XceI	RCATGY	2 cut(s)	39, 494
XhoI	CTCGAG	1 cut(s)	1746
XmnI	GAANNNNTTC	1 cut(s)	828
XspI	CTAG	3 cut(s)	231, 1155, 1629

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.