RLG00000012725

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0019055
Physical Location & Seq
Reverse (-)
21665066 .. 21666193
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012725

Sequence Viewer

Length: 435 bp
ATGTCAATCACCGGTCCAGGTGAGAGGCACTACGACGAGGAGACTGAGCCCGGTGTTGTTTCTGGCGCGCCGTCCAAGGTCTCGTGTGCGCGATCCCTCAGTGTGGCTTCTAACATCTACGACAACACCGCTGCAATCGCCAGTCCGAGTTCGACTCCGACAATCGCCGATCTGAGGGTGTTCACCTTCTCCAAGCTCAAATCAGTGAATCGTCGATTCAGTCGAGCCTTGCTGATCCGAGACTTCAAAGCGCCATCGTCTCGGATTGTTGTTGTTTGCTCCAGCAACGTCCTCTTGAGCGTCATCGTCGTTGTCTCGGATTGCTCACACATGTTTGAGCGGCGGAATAGTCATCGAGTCCCAGGCAGCTGGGGATGCAGAAACGGTGTCATTCTCTCTACTGCTCCTGATCATCAGCTTATGTTTGAAATTTGA

Protein Analysis

145

Amino Acids

15.65

Weight (kDa)

9.13

Isoelectric Point (pI)

57.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019055)

Species Orthologous Gene IDs
rosa_laevigata RLG00000006222 RLG00000006795 RLG00000010090 RLG00000012725 RLG00000024751
rosa_multiflora Rmu_sc0000929.1_g000040 Rmu_sc0001762.1_g000043
rosa_roxburghii Rroxscaffold_5G00374360
rosa_samantha Rh3DG370200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 2 cut(s) 68, 91
AciI CCGC 3 cut(s) 129, 340, 343
AclWI GGATC 2 cut(s) 87, 229
AcsI RAATTY 1 cut(s) 429
AfiI CCNNNNNNNGG 2 cut(s) 103, 174
AflIII ACRYGT 1 cut(s) 330
AgeI ACCGGT 1 cut(s) 11
AgsI TTSAA 2 cut(s) 247, 428
AjnI CCWGG 2 cut(s) 16, 361
AluBI AGCT 3 cut(s) 196, 369, 418
AluI AGCT 3 cut(s) 196, 369, 418
Alw26I GTCTC 5 cut(s) 35, 85, 234, 264, 319
AlwI GGATC 2 cut(s) 87, 229
ApeKI GCWGC 2 cut(s) 131, 366
ApoI RAATTY 1 cut(s) 429
AscI GGCGCGCC 1 cut(s) 66
AsiGI ACCGGT 1 cut(s) 11
AspLEI GCGC 4 cut(s) 68, 70, 91, 253
AspS9I GGNCC 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 32, 175
AvaII GGWCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 51
BauI CACGAG 1 cut(s) 82
BbvI GCAGC 2 cut(s) 118, 378
BccI CCATC 1 cut(s) 262
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 2 cut(s) 18, 363
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 51
BcoDI GTCTC 5 cut(s) 35, 85, 234, 264, 319
BfoI RGCGCY 1 cut(s) 254
BisI GCNGC 3 cut(s) 132, 341, 367
BlsI GCNGC 3 cut(s) 133, 342, 368
Bme1390I CCNGG 3 cut(s) 18, 51, 363
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BmrFI CCNGG 3 cut(s) 18, 51, 363
BmsI GCATC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 139, 171
BpmI CTGGAG 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 316
BpuMI CCSGG 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 75, 361
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 174
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 1 cut(s) 141
BseBI CCWGG 2 cut(s) 18, 363
BseDI CCNNGG 2 cut(s) 75, 361
BseGI GGATG 1 cut(s) 380
BseLI CCNNNNNNNGG 2 cut(s) 103, 174
BseMII CTCAG 3 cut(s) 36, 112, 164
BseNI ACTGG 1 cut(s) 141
BsePI GCGCGC 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 2 cut(s) 118, 378
BseYI CCCAGC 1 cut(s) 369
Bsh1236I CGCG 2 cut(s) 68, 91
BshTI ACCGGT 1 cut(s) 11
BsiSI CCGG 2 cut(s) 12, 51
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 2 cut(s) 103, 174
BsmAI GTCTC 5 cut(s) 35, 85, 234, 264, 319
BsmBI CGTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 344
Bso31I GGTCTC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 4 cut(s) 92, 169, 234, 409
BspACI CCGC 3 cut(s) 129, 340, 343
BspCNI CTCAG 3 cut(s) 37, 111, 165
BspFNI CGCG 2 cut(s) 68, 91
BspPI GGATC 2 cut(s) 87, 229
BspTNI GGTCTC 1 cut(s) 85
BsrBI CCGCTC 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 1 cut(s) 141
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 75, 361
BssHII GCGCGC 1 cut(s) 66
BssMI GATC 4 cut(s) 92, 169, 234, 409
BssSI CACGAG 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 18, 363
Bst4CI ACNGT 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 3 cut(s) 45, 98, 173
BstF5I GGATG 1 cut(s) 380
BstFNI CGCG 2 cut(s) 68, 91
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 4 cut(s) 68, 70, 91, 253
BstKTI GATC 4 cut(s) 95, 172, 237, 412
BstMAI GTCTC 5 cut(s) 35, 85, 234, 264, 319
BstMBI GATC 4 cut(s) 92, 169, 234, 409
BstMWI GCNNNNNNNGC 2 cut(s) 137, 375
BstNI CCWGG 2 cut(s) 18, 363
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 16, 49, 361
BstUI CGCG 2 cut(s) 68, 91
BstV1I GCAGC 2 cut(s) 118, 378
BstXI CCANNNNNNTGG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 380
BtsIMutI CAGTG 2 cut(s) 106, 210
Cac8I GCNNGC 1 cut(s) 68
CfoI GCGC 4 cut(s) 68, 70, 91, 253
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 14
CseI GACGC 1 cut(s) 289
CspAI ACCGGT 1 cut(s) 11
CviAII CATG 1 cut(s) 331
CviJI RGCY 6 cut(s) 49, 107, 196, 227, 369, 418
CviKI_1 RGCY 6 cut(s) 49, 107, 196, 227, 369, 418
DdeI CTNAG 3 cut(s) 45, 98, 173
DpnI GATC 4 cut(s) 94, 171, 236, 411
DpnII GATC 4 cut(s) 92, 169, 234, 409
EciI GGCGGA 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 51
Eco31I GGTCTC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 14
EcoRII CCWGG 2 cut(s) 16, 361
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 1 cut(s) 334
FaiI YATR 2 cut(s) 332, 422
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 330
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 132, 341, 367
FokI GGATG 1 cut(s) 387
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 3 cut(s) 132, 341, 367
GlaI GCGC 4 cut(s) 67, 69, 90, 252
GluI GCNGC 3 cut(s) 132, 341, 367
GsaI CCCAGC 1 cut(s) 373
GsuI CTGGAG 1 cut(s) 265
HaeII RGCGCY 1 cut(s) 254
HapII CCGG 2 cut(s) 12, 51
HgaI GACGC 1 cut(s) 289
HhaI GCGC 4 cut(s) 68, 70, 91, 253
Hin1II CATG 1 cut(s) 334
Hin6I GCGC 4 cut(s) 66, 68, 89, 251
HinP1I GCGC 4 cut(s) 66, 68, 89, 251
HinfI GANTC 4 cut(s) 154, 208, 216, 357
HpaII CCGG 2 cut(s) 12, 51
HphI GGTGA 2 cut(s) 32, 175
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 6 cut(s) 147, 159, 174, 239, 264, 319
Hpy188III TCNNGA 2 cut(s) 295, 407
Hpy8I GTNNAC 1 cut(s) 183
Hpy99I CGWCG 3 cut(s) 38, 216, 311
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 2 cut(s) 134, 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 375
HpyF3I CTNAG 3 cut(s) 45, 98, 173
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 1 cut(s) 334
HspAI GCGC 4 cut(s) 66, 68, 89, 251
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 4 cut(s) 92, 169, 234, 409
LmnI GCTCC 2 cut(s) 284, 409
Lsp1109I GCAGC 2 cut(s) 118, 378
LweI GCATC 1 cut(s) 365
MaeII ACGT 1 cut(s) 288
MalI GATC 4 cut(s) 94, 171, 236, 411
MbiI CCGCTC 1 cut(s) 340
MboI GATC 4 cut(s) 92, 169, 234, 409
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 1 cut(s) 429
MlyI GAGTC 2 cut(s) 148, 366
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 5 cut(s) 18, 31, 107, 168, 302
MspA1I CMGCKG 2 cut(s) 131, 369
MspI CCGG 2 cut(s) 12, 51
MspR9I CCNGG 3 cut(s) 18, 51, 363
MvaI CCWGG 2 cut(s) 18, 363
MvnI CGCG 2 cut(s) 68, 91
MwoI GCNNNNNNNGC 2 cut(s) 137, 375
NciI CCSGG 1 cut(s) 51
NdeII GATC 4 cut(s) 92, 169, 234, 409
NlaIII CATG 1 cut(s) 334
NspI RCATGY 1 cut(s) 334
PalAI GGCGCGCC 1 cut(s) 66
PauI GCGCGC 1 cut(s) 66
PciI ACATGT 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PfeI GAWTC 2 cut(s) 208, 216
PinAI ACCGGT 1 cut(s) 11
PkrI GCNGC 3 cut(s) 133, 342, 368
PleI GAGTC 2 cut(s) 148, 365
PpsI GAGTC 2 cut(s) 148, 365
PscI ACATGT 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 16, 361
PspFI CCCAGC 1 cut(s) 369
PspGI CCWGG 2 cut(s) 16, 361
PspPI GGNCC 1 cut(s) 14
PteI GCGCGC 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 369
SatI GCNGC 3 cut(s) 132, 341, 367
Sau3AI GATC 4 cut(s) 92, 169, 234, 409
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 2 cut(s) 148, 366
ScrFI CCNGG 3 cut(s) 18, 51, 363
SduI GDGCHC 1 cut(s) 51
SetI ASST 7 cut(s) 22, 81, 188, 198, 291, 371, 420
SfaNI GCATC 1 cut(s) 365
SgsI GGCGCGCC 1 cut(s) 66
SinI GGWCC 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
Sse9I AATT 1 cut(s) 429
SsiI CCGC 3 cut(s) 129, 340, 343
StyD4I CCNGG 3 cut(s) 16, 49, 361
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 1 cut(s) 291
TaqI TCGA 4 cut(s) 152, 214, 223, 355
TasI AATT 1 cut(s) 429
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 2 cut(s) 208, 216
TscAI CASTG 2 cut(s) 106, 210
TseI GCWGC 2 cut(s) 131, 366
TspRI CASTG 2 cut(s) 106, 210
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 1 cut(s) 429
XceI RCATGY 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.