RLG00000012746

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018522
Physical Location & Seq
Forward (+)
21960520 .. 21961559
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012746

Sequence Viewer

Length: 960 bp
ATGGCTGAAGAAAACTTTACCGGAATCTGTGCATCTCCACTCCTTATGTTCTCATACTCGGACCCGGATAGTCCTAGTTCTCAAGCCAATATCCGATGCTTGATGACTATATCTGAAGACTACAAGGTTAATTTGTTGAAACCTTTGGATCATCATCGTGCTCAAATCAAGCTACCAGATATTAACATGGTTAAAACTAGTGTTGGAGGGATTAACTCCGGAGTGACGGGCTATGACCATATGATTACCAAGTTTGTCTTATCATCGAACCCTTTTTCGTCACCGGAATGCTCAGTTCTGGTAAGTTACGAGACTGCAAGTGGTGGTTGGGGGTTTTGCAGACTAGGAGAAGATACATGGACGTACGTAGCCTGGACTCTAATACTCGATTTAATTTATCACAACGGGCAGTTTTATGCGATGGACTGTGATGGCAAAATCTTGGTTTGTGATGTTCAAGACCCTGAGCAACGAAACACGACTATTTTGCTGTCAAAGAAGATACCAATGGTACCTATGTATGGGCTGACTACTAGTCAAAGCTTGTACATGGTAGAATCAGCAGGGGCCTTGTTGGTGGTTCTGCAATCTAGGCCAAGAATTGGATCAATTGAAACCACCAGGTTTAGGGTATTTGAGGTGCCGTTTGGTAGAGACCACGGGTGGGAGTGGTCAGATTTGGAGGTAAAGAATTTGGAGAATAGAAGTCTACTCTTGGGATGTGAAAGTTCTTCATTCTCTGTTGATGGCTCAGACTACTACTCTGCAGCATGCAAAGCCAATTGCATATACTTCAACACTAAATGGTGGGAGTGGCTTTCACTTCCAAATACAAACTTTGCTCCGGCTGTTTTTATCTTTAACATGGAAGATGAAAAGGTGGAGCAACTTTCAGTACCTAATATTTTACAAGCCCCACTACATACTTCATGGATACAACCACAACCACCAAGTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

320

Amino Acids

35.82

Weight (kDa)

4.86

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 35 - 266 3.5e-39 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018522)

Species Orthologous Gene IDs
prunus_persica Prupe.6G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0284411 RchiOBHm_Chr6g0284431
rosa_laevigata RLG00000012746 RLG00000024920
rosa_multiflora Rmu_sc0000614.1_g000003 Rmu_sc0004274.1_g000001
rosa_roxburghii Rroxscaffold_1G00014080 Rroxscaffold_6G00418520
rosa_rugosa Rorug03G0051900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 511
AccB1I GGYRCC 2 cut(s) 511, 640
AccB7I CCANNNNNTGG 1 cut(s) 602
AccI GTMKAC 1 cut(s) 709
AccIII TCCGGA 1 cut(s) 218
AclWI GGATC 2 cut(s) 156, 613
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 2 cut(s) 27, 135
AfaI GTAC 4 cut(s) 365, 513, 548, 897
AfiI CCNNNNNNNGG 4 cut(s) 521, 602, 627, 664
AgsI TTSAA 4 cut(s) 139, 458, 614, 796
AhdI GACNNNNNGTC 1 cut(s) 534
AhlI ACTAGT 2 cut(s) 197, 533
AjnI CCWGG 2 cut(s) 371, 620
AluBI AGCT 2 cut(s) 172, 543
AluI AGCT 2 cut(s) 172, 543
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 2 cut(s) 305, 648
AlwI GGATC 2 cut(s) 156, 613
Aor13HI TCCGGA 1 cut(s) 218
AoxI GGCC 2 cut(s) 567, 593
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 1 cut(s) 691
Asp718I GGTACC 1 cut(s) 511
AspS9I GGNCC 2 cut(s) 61, 567
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 273
AvaII GGWCC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 503, 536
BanI GGYRCC 2 cut(s) 511, 640
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 779
BccI CCATC 3 cut(s) 415, 425, 740
BceAI ACGGC 1 cut(s) 628
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 2 cut(s) 373, 622
BciVI GTATCC 1 cut(s) 927
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 305, 648
BcuI ACTAGT 2 cut(s) 197, 533
BfaI CTAG 5 cut(s) 75, 198, 344, 534, 591
BfmI CTRYAG 1 cut(s) 765
BfuI GTATCC 1 cut(s) 927
BisI GCNGC 1 cut(s) 768
BlsI GCNGC 1 cut(s) 769
Bme1390I CCNGG 3 cut(s) 65, 373, 622
Bme18I GGWCC 1 cut(s) 61
BmeRI GACNNNNNGTC 1 cut(s) 534
BmgT120I GGNCC 2 cut(s) 61, 567
BmiI GGNNCC 4 cut(s) 63, 513, 568, 642
BmrFI CCNGG 3 cut(s) 65, 373, 622
BmsI GCATC 2 cut(s) 41, 86
BpiI GAAGAC 1 cut(s) 123
Bpu10I CCTNAGC 1 cut(s) 465
BpuEI CTTGAG 1 cut(s) 66
BpuMI CCSGG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 367
BsaBI GATNNNNATC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 1 cut(s) 658
BsaWI WCCGGW 3 cut(s) 20, 218, 283
Bsc4I CCNNNNNNNGG 4 cut(s) 521, 602, 627, 664
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 218
BseBI CCWGG 2 cut(s) 373, 622
BseDI CCNNGG 1 cut(s) 658
BseGI GGATG 1 cut(s) 725
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 4 cut(s) 521, 602, 627, 664
BseMII CTCAG 3 cut(s) 306, 456, 765
BseXI GCAGC 1 cut(s) 779
BshFI GGCC 2 cut(s) 569, 595
BshNI GGYRCC 2 cut(s) 511, 640
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 5 cut(s) 21, 65, 219, 284, 845
BsiWI CGTACG 1 cut(s) 363
BslI CCNNNNNNNGG 4 cut(s) 521, 602, 627, 664
BsmAI GTCTC 2 cut(s) 305, 648
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 2 cut(s) 569, 595
Bso31I GGTCTC 1 cut(s) 648
Bsp1286I GDGCHC 1 cut(s) 163
Bsp13I TCCGGA 1 cut(s) 218
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 2 cut(s) 148, 605
BspANI GGCC 2 cut(s) 569, 595
BspCNI CTCAG 3 cut(s) 305, 457, 764
BspEI TCCGGA 1 cut(s) 218
BspLI GGNNCC 4 cut(s) 63, 513, 568, 642
BspMAI CTGCAG 1 cut(s) 769
BspPI GGATC 2 cut(s) 156, 613
BspT107I GGYRCC 2 cut(s) 511, 640
BspTNI GGTCTC 1 cut(s) 648
BsrGI TGTACA 1 cut(s) 546
BssECI CCNNGG 1 cut(s) 658
BssMI GATC 2 cut(s) 148, 605
Bst2UI CCWGG 2 cut(s) 373, 622
Bst4CI ACNGT 1 cut(s) 428
BstAUI TGTACA 1 cut(s) 546
BstBAI YACGTR 1 cut(s) 367
BstC8I GCNNGC 1 cut(s) 772
BstDEI CTNAG 3 cut(s) 292, 465, 751
BstDSI CCRYGG 1 cut(s) 658
BstF5I GGATG 1 cut(s) 725
BstKTI GATC 2 cut(s) 151, 608
BstMAI GTCTC 2 cut(s) 305, 648
BstMBI GATC 2 cut(s) 148, 605
BstMWI GCNNNNNNNGC 2 cut(s) 592, 776
BstNI CCWGG 2 cut(s) 373, 622
BstNSI RCATGY 1 cut(s) 774
BstSCI CCNGG 3 cut(s) 63, 371, 620
BstSFI CTRYAG 1 cut(s) 765
BstSNI TACGTA 1 cut(s) 367
BstV1I GCAGC 1 cut(s) 779
BstV2I GAAGAC 1 cut(s) 123
BsuI GTATCC 1 cut(s) 927
BsuRI GGCC 2 cut(s) 569, 595
BtgI CCRYGG 1 cut(s) 658
BtgZI GCGATG 1 cut(s) 434
BtsCI GGATG 1 cut(s) 725
Cac8I GCNNGC 1 cut(s) 772
Cfr13I GGNCC 2 cut(s) 61, 567
CsiI ACCWGGT 1 cut(s) 620
Csp6I GTAC 4 cut(s) 364, 512, 547, 896
CviAII CATG 6 cut(s) 187, 357, 550, 771, 865, 930
CviQI GTAC 4 cut(s) 364, 512, 547, 896
DdeI CTNAG 3 cut(s) 292, 465, 751
DpnI GATC 2 cut(s) 150, 607
DpnII GATC 2 cut(s) 148, 605
DriI GACNNNNNGTC 1 cut(s) 534
Eam1105I GACNNNNNGTC 1 cut(s) 534
Eco105I TACGTA 1 cut(s) 367
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 27, 135
EcoO109I RGGNCCY 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 371, 620
FaeI CATG 6 cut(s) 190, 360, 553, 774, 868, 933
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FatI CATG 6 cut(s) 186, 356, 549, 770, 864, 929
FauNDI CATATG 1 cut(s) 240
FblI GTMKAC 1 cut(s) 709
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 1 cut(s) 732
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 5 cut(s) 75, 198, 344, 534, 591
GluI GCNGC 1 cut(s) 768
HaeIII GGCC 2 cut(s) 569, 595
HapII CCGG 5 cut(s) 21, 65, 219, 284, 845
Hin1II CATG 6 cut(s) 190, 360, 553, 774, 868, 933
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 3 cut(s) 24, 376, 557
HpaII CCGG 5 cut(s) 21, 65, 219, 284, 845
HphI GGTGA 1 cut(s) 273
Hpy166II GTNNAC 1 cut(s) 710
Hpy188I TCNGA 5 cut(s) 61, 95, 115, 676, 754
Hpy188III TCNNGA 2 cut(s) 219, 458
Hpy8I GTNNAC 1 cut(s) 710
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4IV ACGT 2 cut(s) 362, 366
HpyCH4V TGCA 7 cut(s) 32, 317, 339, 586, 767, 774, 786
HpyF10VI GCNNNNNNNGC 2 cut(s) 592, 776
HpyF3I CTNAG 3 cut(s) 292, 465, 751
HpySE526I ACGT 2 cut(s) 362, 366
Hsp92II CATG 6 cut(s) 190, 360, 553, 774, 868, 933
Kpn2I TCCGGA 1 cut(s) 218
KpnI GGTACC 1 cut(s) 515
Kzo9I GATC 2 cut(s) 148, 605
LmnI GCTCC 2 cut(s) 847, 883
Lsp1109I GCAGC 1 cut(s) 779
LweI GCATC 2 cut(s) 41, 86
MabI ACCWGGT 1 cut(s) 620
MaeI CTAG 5 cut(s) 75, 198, 344, 534, 591
MaeII ACGT 2 cut(s) 362, 366
MaeIII GTNAC 3 cut(s) 223, 279, 305
MalI GATC 2 cut(s) 150, 607
MboI GATC 2 cut(s) 148, 605
MboII GAAGA 6 cut(s) 20, 128, 362, 511, 723, 881
MfeI CAATTG 2 cut(s) 609, 781
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 6 cut(s) 130, 393, 600, 609, 691, 781
MlyI GAGTC 1 cut(s) 370
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 3 cut(s) 200, 631, 676
MroI TCCGGA 1 cut(s) 218
MseI TTAA 6 cut(s) 129, 183, 192, 213, 392, 861
MslI CAYNNNNRTG 2 cut(s) 156, 286
MspI CCGG 5 cut(s) 21, 65, 219, 284, 845
MspR9I CCNGG 3 cut(s) 65, 373, 622
MunI CAATTG 2 cut(s) 609, 781
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 2 cut(s) 373, 622
MwoI GCNNNNNNNGC 2 cut(s) 592, 776
NciI CCSGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 240
NdeII GATC 2 cut(s) 148, 605
NlaIII CATG 6 cut(s) 190, 360, 553, 774, 868, 933
NlaIV GGNNCC 4 cut(s) 63, 513, 568, 642
NmuCI GTSAC 2 cut(s) 223, 279
NspI RCATGY 1 cut(s) 774
PaeI GCATGC 1 cut(s) 774
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 24, 557
Pfl23II CGTACG 1 cut(s) 363
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 1 cut(s) 769
PleI GAGTC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 370
Ppu21I YACGTR 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 371, 620
PspGI CCWGG 2 cut(s) 371, 620
PspLI CGTACG 1 cut(s) 363
PspN4I GGNNCC 4 cut(s) 63, 513, 568, 642
PspPI GGNCC 2 cut(s) 61, 567
PstI CTGCAG 1 cut(s) 769
RsaI GTAC 4 cut(s) 365, 513, 548, 897
RsaNI GTAC 4 cut(s) 364, 512, 547, 896
RseI CAYNNNNRTG 2 cut(s) 156, 286
SaqAI TTAA 6 cut(s) 129, 183, 192, 213, 392, 861
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 2 cut(s) 148, 605
Sau96I GGNCC 2 cut(s) 61, 567
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 3 cut(s) 65, 373, 622
SduI GDGCHC 1 cut(s) 163
SexAI ACCWGGT 1 cut(s) 620
SfaNI GCATC 2 cut(s) 41, 86
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 156, 286
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
SnaBI TACGTA 1 cut(s) 367
SpeI ACTAGT 2 cut(s) 197, 533
SphI GCATGC 1 cut(s) 774
Sse9I AATT 6 cut(s) 130, 393, 600, 609, 691, 781
SspI AATATT 1 cut(s) 904
SspMI CTAG 5 cut(s) 75, 198, 344, 534, 591
StyD4I CCNGG 3 cut(s) 63, 371, 620
TaaI ACNGT 1 cut(s) 428
TaiI ACGT 2 cut(s) 365, 369
TaqI TCGA 2 cut(s) 266, 387
TasI AATT 6 cut(s) 130, 393, 600, 609, 691, 781
TatI WGTACW 1 cut(s) 546
TfiI GAWTC 2 cut(s) 24, 557
Tru1I TTAA 6 cut(s) 129, 183, 192, 213, 392, 861
Tru9I TTAA 6 cut(s) 129, 183, 192, 213, 392, 861
TseFI GTSAC 2 cut(s) 223, 279
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 2 cut(s) 223, 279
TspDTI ATGAA 3 cut(s) 723, 888, 918
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 691
XceI RCATGY 1 cut(s) 774
XmiI GTMKAC 1 cut(s) 709
XspI CTAG 5 cut(s) 75, 198, 344, 534, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.