Rroxscaffold_1G00014080

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018522
Physical Location & Seq
Reverse (-)
17420571 .. 17423626
3056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014080.1

Sequence Viewer

Length: 531 bp
ATGCCACTCGCTATTACCGGTTTGAAACTTGACTCGGCGATCCAAGCTCAATGGCATTCGGCATCGGTGGGAGGAGTGTTGCCGGGGAGGAGTTCGATGGAGTCGGCGTCGGTGGAGCTCTACCGGAAGACGGTCGCTCCCCGCCTTGCTTCAACTTCTCAAGGTCCTTGGACCGGACAAGAACAATGGGTATTTGAGGTGTCGTTTGGTAAAGACCACGAGTGGGAGTGGTCAAAGTTGGAGGTAAATAATTTGGAGAATAGAAATGTATTCTTGGGATGTGAAAATTCTTCATTCTCTGTTGATGGCTTAGACTACTACTCTGGATGCAAAGCCAACTGCGTGTATAGATACTTCATGGTTAAATGGTTTGAGCGGCTCTCACTTCCGAATACAAACTTGGATCCGGCTATTTTTTTCTTTAACATGGAAGATGAAAAGGTGGAGCTACTTTCGGTACCTAACGTTTTAGAAGCACCACATGCTTCATGGATACAATTAACCACAACCATGTACCAAGTTTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

176

Amino Acids

19.97

Weight (kDa)

5.06

Isoelectric Point (pI)

21.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 63 - 116 2.4e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018522)

Species Orthologous Gene IDs
prunus_persica Prupe.6G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0284411 RchiOBHm_Chr6g0284431
rosa_laevigata RLG00000012746 RLG00000024920
rosa_multiflora Rmu_sc0000614.1_g000003 Rmu_sc0004274.1_g000001
rosa_roxburghii Rroxscaffold_1G00014080 Rroxscaffold_6G00418520
rosa_rugosa Rorug03G0051900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 1 cut(s) 457
AccBSI CCGCTC 1 cut(s) 376
AciI CCGC 2 cut(s) 142, 376
AclI AACGTT 1 cut(s) 465
AclWI GGATC 3 cut(s) 34, 398, 411
AcsI RAATTY 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 2 cut(s) 459, 515
AfiI CCNNNNNNNGG 3 cut(s) 130, 173, 223
AgeI ACCGGT 1 cut(s) 17
AgsI TTSAA 2 cut(s) 25, 153
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 3 cut(s) 47, 118, 448
AluI AGCT 3 cut(s) 47, 118, 448
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 3 cut(s) 34, 398, 411
ApoI RAATTY 1 cut(s) 286
AsiGI ACCGGT 1 cut(s) 17
Asp718I GGTACC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 164, 171
AsuC2I CCSGG 1 cut(s) 84
AvaII GGWCC 2 cut(s) 164, 171
BamHI GGATCC 1 cut(s) 403
BanI GGYRCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 338, 370
BauI CACGAG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 120
BccI CCATC 2 cut(s) 91, 299
BciVI GTATCC 1 cut(s) 486
BcnI CCSGG 1 cut(s) 84
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 1 cut(s) 377
BlsI GCNGC 1 cut(s) 378
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 2 cut(s) 164, 171
BmgT120I GGNCC 2 cut(s) 164, 171
BmiI GGNNCC 2 cut(s) 405, 459
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 2 cut(s) 71, 317
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 365, 397
BpuEI CTTGAG 1 cut(s) 144
BpuMI CCSGG 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 83, 167
BsaWI WCCGGW 3 cut(s) 17, 123, 173
BsaXI ACNNNNNCTCC 4 cut(s) 92, 121, 122, 151
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 173, 223
Bse118I RCCGGY 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 83, 167
BseGI GGATG 2 cut(s) 284, 332
BseLI CCNNNNNNNGG 3 cut(s) 130, 173, 223
BseRI GAGGAG 2 cut(s) 87, 103
Bsh1285I CGRYCG 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 457
BshTI ACCGGT 1 cut(s) 17
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 5 cut(s) 18, 83, 124, 174, 407
BslI CCNNNNNNNGG 3 cut(s) 130, 173, 223
BsmI GAATGC 1 cut(s) 55
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 2 cut(s) 39, 403
BspACI CCGC 2 cut(s) 142, 376
BspLI GGNNCC 2 cut(s) 405, 459
BspPI GGATC 3 cut(s) 34, 398, 411
BspT107I GGYRCC 1 cut(s) 457
BsrBI CCGCTC 1 cut(s) 376
BsrFI RCCGGY 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 2 cut(s) 83, 167
BssMI GATC 2 cut(s) 39, 403
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 218
BssT1I CCWWGG 1 cut(s) 167
Bst2BI CACGAG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 133
BstACI GRCGYC 1 cut(s) 107
BstAPI GCANNNNNTGC 1 cut(s) 482
BstDEI CTNAG 1 cut(s) 310
BstF5I GGATG 2 cut(s) 284, 332
BstKTI GATC 2 cut(s) 42, 406
BstMBI GATC 2 cut(s) 39, 403
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 2 cut(s) 44, 482
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 82
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuI GTATCC 1 cut(s) 486
BtsCI GGATG 2 cut(s) 284, 332
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 164, 171
CseI GACGC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 458, 514
CspAI ACCGGT 1 cut(s) 17
CviAII CATG 5 cut(s) 358, 427, 482, 489, 511
CviJI RGCY 7 cut(s) 47, 118, 309, 335, 379, 410, 448
CviKI_1 RGCY 7 cut(s) 47, 118, 309, 335, 379, 410, 448
CviQI GTAC 2 cut(s) 458, 514
DdeI CTNAG 1 cut(s) 310
DpnI GATC 2 cut(s) 41, 405
DpnII GATC 2 cut(s) 39, 403
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 2 cut(s) 164, 171
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 5 cut(s) 361, 430, 485, 492, 514
FaiI YATR 6 cut(s) 348, 359, 428, 483, 490, 512
FatI CATG 5 cut(s) 357, 426, 481, 488, 510
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 1 cut(s) 377
FokI GGATG 2 cut(s) 291, 339
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 377
GluI GCNGC 1 cut(s) 377
HapII CCGG 5 cut(s) 18, 83, 124, 174, 407
HgaI GACGC 1 cut(s) 96
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 5 cut(s) 361, 430, 485, 492, 514
HinfI GANTC 2 cut(s) 32, 101
HpaII CCGG 5 cut(s) 18, 83, 124, 174, 407
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 1 cut(s) 324
Hpy99I CGWCG 1 cut(s) 112
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 482
HpyF3I CTNAG 1 cut(s) 310
HpySE526I ACGT 1 cut(s) 465
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 5 cut(s) 361, 430, 485, 492, 514
KpnI GGTACC 1 cut(s) 461
Kzo9I GATC 2 cut(s) 39, 403
LmnI GCTCC 3 cut(s) 115, 142, 445
LpnPI CCDG 6 cut(s) 31, 96, 137, 187, 309, 420
LweI GCATC 2 cut(s) 71, 317
MaeII ACGT 1 cut(s) 465
MalI GATC 2 cut(s) 41, 405
MbiI CCGCTC 1 cut(s) 376
MboI GATC 2 cut(s) 39, 403
MboII GAAGA 3 cut(s) 139, 282, 443
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 3 cut(s) 250, 286, 497
MlyI GAGTC 2 cut(s) 26, 110
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 4 cut(s) 65, 81, 190, 235
MseI TTAA 3 cut(s) 363, 423, 500
MslI CAYNNNNRTG 1 cut(s) 509
MspI CCGG 5 cut(s) 18, 83, 124, 174, 407
MspR9I CCNGG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 44, 482
NciI CCSGG 1 cut(s) 84
NdeII GATC 2 cut(s) 39, 403
NlaIII CATG 5 cut(s) 361, 430, 485, 492, 514
NlaIV GGNNCC 2 cut(s) 405, 459
NmeAIII GCCGAG 1 cut(s) 14
NspI RCATGY 1 cut(s) 485
PctI GAATGC 1 cut(s) 55
PinAI ACCGGT 1 cut(s) 17
PkrI GCNGC 1 cut(s) 378
PleI GAGTC 2 cut(s) 26, 109
PpsI GAGTC 2 cut(s) 26, 109
PpuMI RGGWCCY 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 120
Psp1406I AACGTT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 405, 459
PspPI GGNCC 2 cut(s) 164, 171
PspPPI RGGWCCY 1 cut(s) 164
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 2 cut(s) 459, 515
RsaNI GTAC 2 cut(s) 458, 514
RseI CAYNNNNRTG 1 cut(s) 509
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 363, 423, 500
SatI GCNGC 1 cut(s) 377
Sau3AI GATC 2 cut(s) 39, 403
Sau96I GGNCC 2 cut(s) 164, 171
SchI GAGTC 2 cut(s) 26, 110
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 1 cut(s) 120
SetI ASST 9 cut(s) 49, 120, 166, 201, 246, 444, 450, 463, 468
SfaNI GCATC 2 cut(s) 71, 317
SinI GGWCC 2 cut(s) 164, 171
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
Sse9I AATT 3 cut(s) 250, 286, 497
SsiI CCGC 2 cut(s) 142, 376
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 468
TaqI TCGA 1 cut(s) 95
TasI AATT 3 cut(s) 250, 286, 497
TauI GCSGC 1 cut(s) 379
Tru1I TTAA 3 cut(s) 363, 423, 500
Tru9I TTAA 3 cut(s) 363, 423, 500
TspDTI ATGAA 4 cut(s) 282, 346, 450, 477
VpaK11BI GGWCC 2 cut(s) 164, 171
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.