RLG00000024920

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018522
Physical Location & Seq
Reverse (-)
40342051 .. 40343014
964 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024920

Sequence Viewer

Length: 687 bp
ATGGCTGATTGGTCGGGTCTTCATCAAGACCTTGTTATCTGTATTGCCAAACAGATAGTCTTCTTGAAAGATTTCCATGCTTTTGGTGCAGTTTGTAAACCATGGAGATCCGCCGCCACCAAAGATAACTTCACAGGTCCATCTCCTCTCCTTATGGTCTCAGGGTGGGACGGAAGTAGTACTGAAGCCAATATCCGCAAACTTGTCCACTTAAAAGATCAGCCAGAGATATATATGAATTATAAGAAGCAGCGTTGTTGTTATTATTCTTCCCTAGGATGGTTGATGAGTATATCTGAAGATTTCAAGGTTAGTTTGTTGAACCCTTTTGATCACCATGCTCGAATCAAGCTGCCGAATATTAACATGCTTAAAGCTAGTGTTGGAGAGGTAAAGTTGCCGCGGGTTCTTCACTATGACCGTATGATTAGCAAGTTTGTCTTATCATCGTGCCCCTTCTCATCATCGGATTGCATTGTCGTGCTTTACGCGGTGGACATCAAAGGCAACATCTTGATTTGTGATATCCAAGACCTTGAGCAACCAAAAACGAGCGTTCTTCTATCAAAGGGGATACCAATGGAACCTATATACGGGGGTAGTCGTCAAAGAATCTACATAGTAGAATCAGCAGGAGTCTTGTTGGTGGTGTTTGAGCCCAAAAGCAATTTTGGCAAGATCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

229

Amino Acids

25.64

Weight (kDa)

8.77

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 75 - 162 7.5e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018522)

Species Orthologous Gene IDs
prunus_persica Prupe.6G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0284411 RchiOBHm_Chr6g0284431
rosa_laevigata RLG00000012746 RLG00000024920
rosa_multiflora Rmu_sc0000614.1_g000003 Rmu_sc0004274.1_g000001
rosa_roxburghii Rroxscaffold_1G00014080 Rroxscaffold_6G00418520
rosa_rugosa Rorug03G0051900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 243
AccII CGCG 2 cut(s) 403, 491
AciI CCGC 6 cut(s) 111, 114, 196, 401, 403, 491
AclWI GGATC 2 cut(s) 102, 673
AcuI CTGAAG 2 cut(s) 204, 318
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 2 cut(s) 279, 593
AgsI TTSAA 3 cut(s) 67, 307, 322
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 2 cut(s) 352, 377
AluI AGCT 2 cut(s) 352, 377
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 2 cut(s) 102, 673
ApeKI GCWGC 2 cut(s) 250, 352
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
Asp700I GAANNNNTTC 1 cut(s) 71
AspA2I CCTAGG 1 cut(s) 274
AspS9I GGNCC 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 137
AvrII CCTAGG 1 cut(s) 274
BaeGI GKGCMC 1 cut(s) 455
BanII GRGCYC 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 11, 52
BbvI GCAGC 2 cut(s) 262, 339
BccI CCATC 2 cut(s) 148, 273
BciVI GTATCC 1 cut(s) 567
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 2 cut(s) 275, 378
BfuI GTATCC 1 cut(s) 567
BisI GCNGC 4 cut(s) 114, 251, 353, 401
BlnI CCTAGG 1 cut(s) 274
BlsI GCNGC 4 cut(s) 115, 252, 354, 402
BmcAI AGTACT 1 cut(s) 181
Bme18I GGWCC 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 585
BpiI GAAGAC 2 cut(s) 11, 52
BpuEI CTTGAG 1 cut(s) 557
BsaI GGTCTC 1 cut(s) 163
BsaJI CCNNGG 3 cut(s) 101, 274, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 593
BseDI CCNNGG 3 cut(s) 101, 274, 401
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 279, 593
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 135
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 2 cut(s) 262, 339
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 2 cut(s) 403, 491
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 279, 593
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 182
Bso31I GGTCTC 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 455, 660
Bsp143I GATC 4 cut(s) 107, 217, 331, 678
Bsp19I CCATGG 1 cut(s) 101
BspACI CCGC 6 cut(s) 111, 114, 196, 401, 403, 491
BspCNI CTCAG 1 cut(s) 173
BspFNI CGCG 2 cut(s) 403, 491
BspLI GGNNCC 1 cut(s) 585
BspPI GGATC 2 cut(s) 102, 673
BspTNI GGTCTC 1 cut(s) 163
BssECI CCNNGG 3 cut(s) 101, 274, 401
BssMI GATC 4 cut(s) 107, 217, 331, 678
BssT1I CCWWGG 2 cut(s) 101, 274
Bst4CI ACNGT 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 160
BstDSI CCRYGG 2 cut(s) 101, 401
BstF5I GGATG 1 cut(s) 284
BstFNI CGCG 2 cut(s) 403, 491
BstKTI GATC 4 cut(s) 110, 220, 334, 681
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 4 cut(s) 107, 217, 331, 678
BstMWI GCNNNNNNNGC 2 cut(s) 86, 672
BstNSI RCATGY 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 455
BstUI CGCG 2 cut(s) 403, 491
BstV1I GCAGC 2 cut(s) 262, 339
BstV2I GAAGAC 2 cut(s) 11, 52
BstX2I RGATCY 2 cut(s) 107, 678
BstXI CCANNNNNNTGG 1 cut(s) 83
BstYI RGATCY 2 cut(s) 107, 678
BsuI GTATCC 1 cut(s) 567
BtgI CCRYGG 2 cut(s) 101, 401
BtsCI GGATG 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 137
Cfr42I CCGCGG 1 cut(s) 404
Csp6I GTAC 1 cut(s) 180
CviAII CATG 4 cut(s) 77, 102, 338, 367
CviJI RGCY 6 cut(s) 5, 188, 223, 352, 377, 658
CviKI_1 RGCY 6 cut(s) 5, 188, 223, 352, 377, 658
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 1 cut(s) 160
DpnI GATC 4 cut(s) 109, 219, 333, 680
DpnII GATC 4 cut(s) 107, 217, 331, 678
EciI GGCGGA 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 101, 274
Eco24I GRGCYC 1 cut(s) 660
Eco31I GGTCTC 1 cut(s) 163
Eco32I GATATC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 204, 318
EcoRV GATATC 1 cut(s) 526
EcoT14I CCWWGG 2 cut(s) 101, 274
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 2 cut(s) 101, 274
FaeI CATG 4 cut(s) 80, 105, 341, 370
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 182
FatI CATG 4 cut(s) 76, 101, 337, 366
FauI CCCGC 1 cut(s) 396
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 4 cut(s) 114, 251, 353, 401
FokI GGATG 1 cut(s) 291
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 4 cut(s) 114, 251, 353, 401
FspBI CTAG 2 cut(s) 275, 378
GluI GCNGC 4 cut(s) 114, 251, 353, 401
Hin1II CATG 4 cut(s) 80, 105, 341, 370
HinfI GANTC 4 cut(s) 345, 612, 626, 636
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 98, 208, 496
Hpy188I TCNGA 2 cut(s) 298, 469
Hpy188III TCNNGA 3 cut(s) 26, 64, 514
Hpy8I GTNNAC 3 cut(s) 98, 208, 496
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4V TGCA 2 cut(s) 89, 474
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 672
HpyF3I CTNAG 1 cut(s) 160
Hsp92II CATG 4 cut(s) 80, 105, 341, 370
Ksp22I TGATCA 1 cut(s) 331
KspI CCGCGG 1 cut(s) 404
Kzo9I GATC 4 cut(s) 107, 217, 331, 678
LpnPI CCDG 4 cut(s) 120, 147, 237, 618
Lsp1109I GCAGC 2 cut(s) 262, 339
MaeI CTAG 2 cut(s) 275, 378
MalI GATC 4 cut(s) 109, 219, 333, 680
MboI GATC 4 cut(s) 107, 217, 331, 678
MboII GAAGA 6 cut(s) 11, 52, 261, 311, 401, 551
MflI RGATCY 2 cut(s) 107, 678
MhlI GDGCHC 2 cut(s) 455, 660
MluCI AATT 2 cut(s) 238, 667
MlyI GAGTC 1 cut(s) 645
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 2 cut(s) 156, 382
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 3 cut(s) 212, 363, 372
MslI CAYNNNNRTG 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 403
MvnI CGCG 2 cut(s) 403, 491
MwoI GCNNNNNNNGC 2 cut(s) 86, 672
NcoI CCATGG 1 cut(s) 101
NdeII GATC 4 cut(s) 107, 217, 331, 678
NlaIII CATG 4 cut(s) 80, 105, 341, 370
NlaIV GGNNCC 1 cut(s) 585
NspI RCATGY 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 345, 612, 626
PkrI GCNGC 4 cut(s) 115, 252, 354, 402
PleI GAGTC 1 cut(s) 644
PpsI GAGTC 1 cut(s) 644
PsiI TTATAA 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 585
PspPI GGNCC 1 cut(s) 137
PsuI RGATCY 2 cut(s) 107, 678
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
RseI CAYNNNNRTG 1 cut(s) 479
SacII CCGCGG 1 cut(s) 404
SaqAI TTAA 3 cut(s) 212, 363, 372
SatI GCNGC 4 cut(s) 114, 251, 353, 401
Sau3AI GATC 4 cut(s) 107, 217, 331, 678
Sau96I GGNCC 1 cut(s) 137
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 1 cut(s) 645
SduI GDGCHC 2 cut(s) 455, 660
SetI ASST 8 cut(s) 33, 139, 312, 354, 379, 393, 537, 589
Sfr303I CCGCGG 1 cut(s) 404
SgrBI CCGCGG 1 cut(s) 404
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 2 cut(s) 238, 667
SsiI CCGC 6 cut(s) 111, 114, 196, 401, 403, 491
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 275, 378
StyI CCWWGG 2 cut(s) 101, 274
TaaI ACNGT 1 cut(s) 422
TaqI TCGA 1 cut(s) 343
TasI AATT 2 cut(s) 238, 667
TatI WGTACW 1 cut(s) 179
TauI GCSGC 2 cut(s) 116, 403
TfiI GAWTC 3 cut(s) 345, 612, 626
Tru1I TTAA 3 cut(s) 212, 363, 372
Tru9I TTAA 3 cut(s) 212, 363, 372
TseI GCWGC 2 cut(s) 250, 352
TspDTI ATGAA 2 cut(s) 11, 251
TspGWI ACGGA 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 137
XceI RCATGY 1 cut(s) 370
XmaJI CCTAGG 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 2 cut(s) 275, 378
ZrmI AGTACT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.