RLG00000014563

cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004973
Physical Location & Seq
Forward (+)
54778113 .. 54779822
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014563

Sequence Viewer

Length: 1392 bp
ATGTCACCAAATTGTCTCCACAGTAAAAGTTTTAGCATCACCGGAGGCAGGTGGCACCAGGACCAGAGCTCGAGCTCGATCTTCTGTTTTGTTCCGATTCTGGATCTCGCCAAGGCCTTCGCTCGGGTCAAACTTAAACTCAGCCGAGCCGATCGAAGCCGATTCATGATGGATGGATCGTGGACTGCAACTGTGATGCGGCGAATTCACGAGCGCGTAGTTGGATCCAGCCGTACTGCTGCTATCTCCTCCACCAGTCATGTGTGGCTTCTCGGCGTGTGCTATAAGTTCGATGAGTCCGAGTCCTCTCCACTTGCATTCGAACACGATTTTTCGTCGAGAATCTTGATGACGTATCGAAGAGGATTCGATTCGATTGAGGGTGGGGATTCCAAGTGTTTTAGCAGCGATGTGAATTGGGGTTGTATGATTCGGAGCAGTCAGATGCTTGTGGCTCAGGCATTGCTTTTCCACAGGTTGGGAAGATCTTGGAGGCGGCCTCTCGACAAGTCTTGCAGCTTGGATCAAGAGTATGTTGAGATCTTGCGTCAATTTGGTGATTCCGAAGCTGCGGCCTTCTCCATCCACAATCTGATTGAGGCTGGCAAGGCTTATAACCTTGCTGCCGGTTCCTGGGTTGGCCCTTATGCTATGTGCCGCTCCTGGGAGACTCTCGTGTGCAAGCAGAGGGAGGCCGAGGCGGCGGCAGCTCAGGAGCTTGATGAACAGCCACTGCCGATGGCTGTTTATATTGTTTCTGGAGACGAAAATGGGGAGAGAGGCGGTGCTCCGGTTGTGTGTGTTGAAGATGCATTTAGACATTGTTCAGAGTTTTCGAGAGGTCGGGTTGATTGGACGCCGATTCTTTTGCTGGTTCCTCTGGTTCTTGGACTTGAAGAACTCAATCCCAGGTACATTCCATCCTTGCGAGCTACGTTTACCTTTCCTCAGAGTGTTGGCATCATGGGTGGCAGACCTGGTGTATCAACTTACATTATTGGAGTGCAAGATGAAAATGCAATTTACCTTGATCCACATCTAGTTCAGCCTGTAGTCAATTTGAGTAGTAATGATTTAGATAGTGATGCTACTTCATCTTACCACTGCAATGTTATTCGCCACATTCCTTTAGATACAATTGATCCATCCCTGGCAATTGGATTCTATTGCCGTGACAAAGATGATTTTGATGATTTTTGTCTACGAGCTTCGAAGCTGGCAGATGAATCAAATGGTGCTCCGCTGTTTACTGTGAGTGCTGAAACACATAATTTGTCCAAGCCACCTAGCCAGAGTGATGATGGTTTGGGTGATAATGGTGGCGATCAAAACAATGAAGATGCAGATGGAGATGGAAGCGCACATGAAGAAGATGACTGGCAACTCCTCTGA

Protein Analysis

464

Amino Acids

51.23

Weight (kDa)

4.97

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C54 PF03416 106 - 400 5.6e-90 Peptidase C54 catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AarI CACCTGC 1 cut(s) 39
Acc36I ACCTGC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 54
AccB7I CCANNNNNTGG 1 cut(s) 478
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 1 cut(s) 1201
AccII CGCG 1 cut(s) 216
AciI CCGC 8 cut(s) 199, 496, 572, 658, 701, 704, 783, 1241
AclWI GGATC 7 cut(s) 111, 184, 219, 232, 531, 1025, 1136
AcsI RAATTY 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 857
AfaI GTAC 2 cut(s) 235, 914
AfiI CCNNNNNNNGG 5 cut(s) 48, 123, 478, 633, 664
AgsI TTSAA 2 cut(s) 806, 896
AjnI CCWGG 6 cut(s) 57, 632, 662, 908, 976, 1149
AluBI AGCT 9 cut(s) 69, 75, 519, 569, 710, 718, 932, 1208, 1216
AluI AGCT 9 cut(s) 69, 75, 519, 569, 710, 718, 932, 1208, 1216
Alw21I GWGCWC 4 cut(s) 71, 77, 790, 1240
Alw26I GTCTC 3 cut(s) 20, 662, 756
AlwI GGATC 7 cut(s) 111, 184, 219, 232, 531, 1025, 1136
AlwNI CAGNNNCTG 1 cut(s) 733
Ama87I CYCGRG 2 cut(s) 70, 123
AoxI GGCC 5 cut(s) 114, 497, 573, 640, 693
ApeKI GCWGC 6 cut(s) 239, 405, 516, 569, 623, 707
ApoI RAATTY 1 cut(s) 204
AspLEI GCGC 2 cut(s) 216, 1361
AspS9I GGNCC 2 cut(s) 61, 641
AsuHPI GGTGA 3 cut(s) 31, 569, 1322
AsuII TTCGAA 2 cut(s) 321, 1211
AvaI CYCGRG 2 cut(s) 70, 123
AvaII GGWCC 1 cut(s) 61
BamHI GGATCC 1 cut(s) 224
BanI GGYRCC 1 cut(s) 54
BanII GRGCYC 2 cut(s) 71, 77
BauI CACGAG 2 cut(s) 209, 674
Bbv12I GWGCWC 4 cut(s) 71, 77, 790, 1240
BbvI GCAGC 6 cut(s) 226, 417, 528, 556, 610, 719
BccI CCATC 9 cut(s) 163, 167, 590, 733, 928, 1153, 1295, 1340, 1346
BceAI ACGGC 2 cut(s) 216, 1155
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BciT130I CCWGG 6 cut(s) 59, 634, 664, 910, 978, 1151
BcoDI GTCTC 3 cut(s) 20, 662, 756
BfaI CTAG 2 cut(s) 1040, 1287
BfmI CTRYAG 1 cut(s) 1050
BfuAI ACCTGC 1 cut(s) 39
BglI GCCNNNNNGGC 1 cut(s) 701
BglII AGATCT 2 cut(s) 485, 540
Bme1390I CCNGG 6 cut(s) 59, 634, 664, 910, 978, 1151
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 2 cut(s) 70, 123
BmgT120I GGNCC 2 cut(s) 61, 641
BmiI GGNNCC 4 cut(s) 56, 226, 631, 876
BmrFI CCNGG 6 cut(s) 59, 634, 664, 910, 978, 1151
BmsI GCATC 7 cut(s) 45, 186, 435, 799, 969, 1075, 1330
BplI GAGNNNNNCTC 2 cut(s) 484, 516
BpmI CTGGAG 1 cut(s) 780
Bpu10I CCTNAGC 2 cut(s) 456, 711
Bpu14I TTCGAA 2 cut(s) 321, 1211
BsaBI GATNNNNATC 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 857
BsaJI CCNNGG 6 cut(s) 111, 633, 663, 696, 908, 1149
BsaWI WCCGGW 2 cut(s) 41, 790
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 123, 478, 633, 664
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 2 cut(s) 255, 1382
Bse3DI GCAATG 2 cut(s) 461, 1114
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 6 cut(s) 59, 634, 664, 910, 978, 1151
BseDI CCNNGG 6 cut(s) 111, 633, 663, 696, 908, 1149
BseGI GGATG 4 cut(s) 178, 582, 920, 1145
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 5 cut(s) 48, 123, 478, 633, 664
BseMI GCAATG 2 cut(s) 461, 1114
BseMII CTCAG 4 cut(s) 154, 470, 725, 962
BseNI ACTGG 2 cut(s) 255, 1382
BseRI GAGGAG 2 cut(s) 238, 1376
BseXI GCAGC 6 cut(s) 226, 417, 528, 556, 610, 719
Bsh1236I CGCG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 5 cut(s) 116, 499, 575, 642, 695
BshNI GGYRCC 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 4 cut(s) 71, 77, 790, 1240
BsiHKCI CYCGRG 2 cut(s) 70, 123
BsiSI CCGG 3 cut(s) 42, 627, 791
BslI CCNNNNNNNGG 5 cut(s) 48, 123, 478, 633, 664
BsmAI GTCTC 3 cut(s) 20, 662, 756
BsmBI CGTCTC 1 cut(s) 756
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 5 cut(s) 116, 499, 575, 642, 695
BsoBI CYCGRG 2 cut(s) 70, 123
Bsp119I TTCGAA 2 cut(s) 321, 1211
Bsp1286I GDGCHC 4 cut(s) 71, 77, 790, 1240
BspACI CCGC 8 cut(s) 199, 496, 572, 658, 701, 704, 783, 1241
BspANI GGCC 5 cut(s) 116, 499, 575, 642, 695
BspCNI CTCAG 4 cut(s) 153, 469, 724, 961
BspFNI CGCG 1 cut(s) 216
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 4 cut(s) 56, 226, 631, 876
BspMI ACCTGC 1 cut(s) 39
BspPI GGATC 7 cut(s) 111, 184, 219, 232, 531, 1025, 1136
BspT104I TTCGAA 2 cut(s) 321, 1211
BspT107I GGYRCC 1 cut(s) 54
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 2 cut(s) 461, 1114
BsrFI RCCGGY 1 cut(s) 626
BsrI ACTGG 2 cut(s) 255, 1382
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 6 cut(s) 111, 633, 663, 696, 908, 1149
BssNI GRCGYC 1 cut(s) 857
BssSI CACGAG 2 cut(s) 209, 674
BssT1I CCWWGG 1 cut(s) 111
Bst2BI CACGAG 2 cut(s) 209, 674
Bst2UI CCWGG 6 cut(s) 59, 634, 664, 910, 978, 1151
Bst4CI ACNGT 3 cut(s) 23, 193, 1252
Bst6I CTCTTC 1 cut(s) 355
BstACI GRCGYC 1 cut(s) 857
BstBI TTCGAA 2 cut(s) 321, 1211
BstC8I GCNNGC 4 cut(s) 604, 683, 930, 1218
BstDEI CTNAG 4 cut(s) 140, 456, 711, 948
BstF5I GGATG 4 cut(s) 178, 582, 920, 1145
BstFNI CGCG 1 cut(s) 216
BstHHI GCGC 2 cut(s) 216, 1361
BstMAI GTCTC 3 cut(s) 20, 662, 756
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 608, 701, 707
BstNI CCWGG 6 cut(s) 59, 634, 664, 910, 978, 1151
BstSCI CCNGG 6 cut(s) 57, 632, 662, 908, 976, 1149
BstSFI CTRYAG 1 cut(s) 1050
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 6 cut(s) 226, 417, 528, 556, 610, 719
BstX2I RGATCY 4 cut(s) 103, 224, 485, 540
BstYI RGATCY 4 cut(s) 103, 224, 485, 540
BsuRI GGCC 5 cut(s) 116, 499, 575, 642, 695
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 4 cut(s) 178, 582, 920, 1145
BtsI GCAGTG 2 cut(s) 731, 1102
BtsIMutI CAGTG 2 cut(s) 731, 1102
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 4 cut(s) 604, 683, 930, 1218
CaiI CAGNNNCTG 1 cut(s) 733
CciI TCATGA 1 cut(s) 165
CfoI GCGC 2 cut(s) 216, 1361
Cfr10I RCCGGY 1 cut(s) 626
Cfr13I GGNCC 2 cut(s) 61, 641
CseI GACGC 2 cut(s) 536, 865
CsiI ACCWGGT 1 cut(s) 976
Csp6I GTAC 2 cut(s) 234, 913
CviAII CATG 4 cut(s) 166, 260, 964, 1364
CviQI GTAC 2 cut(s) 234, 913
DdeI CTNAG 4 cut(s) 140, 456, 711, 948
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
Ecl136II GAGCTC 2 cut(s) 69, 75
Eco130I CCWWGG 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 116
Eco24I GRGCYC 2 cut(s) 71, 77
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 2 cut(s) 69, 75
Eco88I CYCGRG 2 cut(s) 70, 123
EcoICRI GAGCTC 2 cut(s) 69, 75
EcoRI GAATTC 1 cut(s) 204
EcoRII CCWGG 6 cut(s) 57, 632, 662, 908, 976, 1149
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 814
EcoT38I GRGCYC 2 cut(s) 71, 77
ErhI CCWWGG 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 756
FaeI CATG 4 cut(s) 169, 263, 967, 1367
FatI CATG 4 cut(s) 165, 259, 963, 1363
FblI GTMKAC 1 cut(s) 1201
FokI GGATG 4 cut(s) 185, 569, 907, 1132
FriOI GRGCYC 2 cut(s) 71, 77
FspBI CTAG 2 cut(s) 1040, 1287
GlaI GCGC 2 cut(s) 215, 1360
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 5 cut(s) 116, 499, 575, 642, 695
HapII CCGG 3 cut(s) 42, 627, 791
HgaI GACGC 2 cut(s) 536, 865
HhaI GCGC 2 cut(s) 216, 1361
Hin1I GRCGYC 1 cut(s) 857
Hin1II CATG 4 cut(s) 169, 263, 967, 1367
Hin6I GCGC 2 cut(s) 214, 1359
HinP1I GCGC 2 cut(s) 214, 1359
HpaII CCGG 3 cut(s) 42, 627, 791
HphI GGTGA 3 cut(s) 31, 569, 1322
Hpy166II GTNNAC 4 cut(s) 183, 939, 1202, 1248
Hpy188I TCNGA 9 cut(s) 96, 301, 435, 444, 565, 594, 829, 951, 1391
Hpy8I GTNNAC 4 cut(s) 183, 939, 1202, 1248
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 2 cut(s) 127, 586
HpyCH4III ACNGT 3 cut(s) 23, 193, 1252
HpyCH4IV ACGT 2 cut(s) 353, 935
HpyCH4V TGCA 9 cut(s) 188, 317, 516, 681, 812, 1006, 1019, 1107, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 701, 707
HpyF3I CTNAG 4 cut(s) 140, 456, 711, 948
HpySE526I ACGT 2 cut(s) 353, 935
Hsp92I GRCGYC 1 cut(s) 857
Hsp92II CATG 4 cut(s) 169, 263, 967, 1367
HspAI GCGC 2 cut(s) 214, 1359
LmnI GCTCC 5 cut(s) 435, 665, 715, 793, 1243
Lsp1109I GCAGC 6 cut(s) 226, 417, 528, 556, 610, 719
LweI GCATC 7 cut(s) 45, 186, 435, 799, 969, 1075, 1330
MabI ACCWGGT 1 cut(s) 976
MaeI CTAG 2 cut(s) 1040, 1287
MaeII ACGT 2 cut(s) 353, 935
MaeIII GTNAC 2 cut(s) 3, 1172
MbiI CCGCTC 1 cut(s) 660
MboII GAAGA 8 cut(s) 73, 372, 495, 818, 908, 1349, 1379, 1382
MfeI CAATTG 2 cut(s) 1137, 1155
MflI RGATCY 4 cut(s) 103, 224, 485, 540
MhlI GDGCHC 4 cut(s) 71, 77, 790, 1240
MluCI AATT 9 cut(s) 10, 204, 415, 551, 1020, 1057, 1137, 1155, 1270
MlyI GAGTC 3 cut(s) 305, 311, 664
MmeI TCCRAC 1 cut(s) 202
Mph1103I ATGCAT 1 cut(s) 814
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 1 cut(s) 1107
MspA1I CMGCKG 1 cut(s) 1243
MspI CCGG 3 cut(s) 42, 627, 791
MspR9I CCNGG 6 cut(s) 59, 634, 664, 910, 978, 1151
MunI CAATTG 2 cut(s) 1137, 1155
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 6 cut(s) 59, 634, 664, 910, 978, 1151
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 3 cut(s) 608, 701, 707
NlaIII CATG 4 cut(s) 169, 263, 967, 1367
NlaIV GGNNCC 4 cut(s) 56, 226, 631, 876
NmeAIII GCCGAG 3 cut(s) 170, 252, 721
NmuCI GTSAC 2 cut(s) 3, 1172
NsiI ATGCAT 1 cut(s) 814
NspV TTCGAA 2 cut(s) 321, 1211
PaeR7I CTCGAG 1 cut(s) 70
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 39
PceI AGGCCT 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 317
PflMI CCANNNNNTGG 1 cut(s) 478
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 3 cut(s) 304, 310, 664
PpsI GAGTC 3 cut(s) 304, 310, 664
PsiI TTATAA 1 cut(s) 615
Psp124BI GAGCTC 2 cut(s) 71, 77
Psp6I CCWGG 6 cut(s) 57, 632, 662, 908, 976, 1149
PspGI CCWGG 6 cut(s) 57, 632, 662, 908, 976, 1149
PspN4I GGNNCC 4 cut(s) 56, 226, 631, 876
PspPI GGNCC 2 cut(s) 61, 641
PspXI VCTCGAGB 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 733
PsuI RGATCY 4 cut(s) 103, 224, 485, 540
PvuI CGATCG 1 cut(s) 154
RsaI GTAC 2 cut(s) 235, 914
RsaNI GTAC 2 cut(s) 234, 913
RseI CAYNNNNRTG 1 cut(s) 1107
SacI GAGCTC 2 cut(s) 71, 77
SaqAI TTAA 1 cut(s) 135
Sau96I GGNCC 2 cut(s) 61, 641
SchI GAGTC 3 cut(s) 305, 311, 664
ScrFI CCNGG 6 cut(s) 59, 634, 664, 910, 978, 1151
SduI GDGCHC 4 cut(s) 71, 77, 790, 1240
SexAI ACCWGGT 1 cut(s) 976
SfaNI GCATC 7 cut(s) 45, 186, 435, 799, 969, 1075, 1330
SfcI CTRYAG 1 cut(s) 1050
Sfr274I CTCGAG 1 cut(s) 70
SfuI TTCGAA 2 cut(s) 321, 1211
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 1107
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
Sse9I AATT 9 cut(s) 10, 204, 415, 551, 1020, 1057, 1137, 1155, 1270
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 8 cut(s) 199, 496, 572, 658, 701, 704, 783, 1241
SspMI CTAG 2 cut(s) 1040, 1287
SstI GAGCTC 2 cut(s) 71, 77
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 6 cut(s) 57, 632, 662, 908, 976, 1149
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 3 cut(s) 23, 193, 1252
TaiI ACGT 2 cut(s) 356, 938
TasI AATT 9 cut(s) 10, 204, 415, 551, 1020, 1057, 1137, 1155, 1270
TauI GCSGC 6 cut(s) 202, 499, 575, 660, 704, 707
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 2 cut(s) 738, 1109
TseFI GTSAC 2 cut(s) 3, 1172
TseI GCWGC 6 cut(s) 239, 405, 516, 569, 623, 707
Tsp45I GTSAC 2 cut(s) 3, 1172
TspDTI ATGAA 7 cut(s) 154, 738, 1026, 1083, 1239, 1350, 1380
TspRI CASTG 2 cut(s) 738, 1109
Van91I CCANNNNNTGG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 204
XcmI CCANNNNNNNNNTGG 1 cut(s) 1298
XhoI CTCGAG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 1201
XspI CTAG 2 cut(s) 1040, 1287
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.