RLG00000015910

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008018
Physical Location & Seq
Forward (+)
2385379 .. 2387426
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015910

Sequence Viewer

Length: 1272 bp
ATGCACCAAAACGGCGTCGTTGTTCTCGACAACGGCGGCGGCCTAATCAAAGCCGGGTACGGCGGCGAGCGCGACCCCGCCGCCATAATCCCCAACTGCGTCTACCGCCCTCTCTCCTCCAAAAAATGGCTCACCCCTTCCCCCACCGACCCCATCGACCTCACCTCCGCCGCCGTCCGCCGCCCCATCGACCGCGGCTACCTTATCAACCCGGACCTCCAGCGAGAGATCTGGGCCCACCTCTTCTCCCAAGTCCTCCGGGTCAACCCGGCCCAATCCTCCCTCCTCCTAACCGAGCCCCTCTTCACTCTCCCTTCCATCCAACGCGCCGCCGACGAGCTCGTCTTCGAGGACTTCAACTTCGCCGCCCTCTACGTGGCCCACGCGCCCTCCCTGGCCCACCTCTACGAGGCGAGCCGCCGGTCCGTTAAGGCCCAATGCAGCCTCGTCGTCGATTGTGGATTCTCGTTCACCCACGCCGTGCCGGTGTTCCAGAACTTTCCGATCAACTACGCCGCGAAGAGAATTGACCTGGGGGGAAAGGCGTTGACCAATTACCTCAAGGAGCTCGTTTCGTACCGCTCTGTGAATGTTATGGACGAAACGTTTCTTATTGACGATGTGAAGGAGAAGCTCTGCTTTGTCTCCATGAATGTGACTCGTGACCTCCAGATTGCAAAGAACCATGGAAAGGACAATGCTTTCAGGTGCACTTATGTGTTGCCTGATGGTGTCACGCATACACAAGGGTATGTTAAAGACCCAGAAGAGGCAAAGAGGTACTTGGCCATAGGAGACGGTGATTCGCTTCAAGAAGTGACCAAGAAGATGGATGTGAATCGGGTTGAAGTTGTGGACAAGTTGGAGGAGCGAAAGAAGATTGATTTGAGCAAGAATGAATTCGACTTGACAAATGAAAGGTTCCTTGTGCCGGAGATGATCTTCCATCCTGCTGATTTGGGAATGAATCAGGCTGGACTAGCGGAATGCATTGTTCGAGCTGTCAGTTCATGCCACCCTCATCTTCACCCTGTACTTTATGAAAGCATCATTTTAACTGGCGGAAGCACATTGTTTCCTCGACTTGCTGATAGACTGTATGCACTGATCTCCTTTCCATATAAGTTTATAACTCCCCAAGAAGACCCCATACTAGGTGTTTGGCGAGGGGGATCAGTTTTGGCATCAAGTCCTGATTTTGAAGCAATGTGTGTTACCAAGGCTGAGTATGAGGAGCTTGGATCTGCTCGATGTCGGAAGAGATTCTTTTAA

Protein Analysis

424

Amino Acids

47.33

Weight (kDa)

6.15

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 3 - 423 4.3e-87 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1130
AasI GACNNNNNNGTC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 126
AccBSI CCGCTC 1 cut(s) 582
AccI GTMKAC 1 cut(s) 102
AccII CGCG 5 cut(s) 72, 195, 327, 386, 518
AclI AACGTT 1 cut(s) 605
AclWI GGATC 2 cut(s) 1180, 1249
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 1 cut(s) 899
AcyI GRCGYC 1 cut(s) 15
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 4 cut(s) 59, 578, 782, 1035
AfiI CCNNNNNNNGG 7 cut(s) 126, 376, 409, 691, 769, 931, 1154
AgsI TTSAA 4 cut(s) 358, 812, 848, 1202
AjnI CCWGG 2 cut(s) 393, 531
AleI CACNNNNGTG 1 cut(s) 716
AluBI AGCT 5 cut(s) 340, 568, 634, 1001, 1237
AluI AGCT 5 cut(s) 340, 568, 634, 1001, 1237
Alw21I GWGCWC 3 cut(s) 342, 570, 713
Alw26I GTCTC 2 cut(s) 649, 789
Alw44I GTGCAC 1 cut(s) 709
AlwI GGATC 2 cut(s) 1180, 1249
AoxI GGCC 7 cut(s) 40, 234, 270, 378, 396, 432, 786
ApaI GGGCCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 709
ApeKI GCWGC 1 cut(s) 441
ApoI RAATTY 1 cut(s) 899
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
Asp700I GAANNNNTTC 3 cut(s) 606, 899, 1262
AspLEI GCGC 3 cut(s) 72, 329, 388
AspS9I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
AsuC2I CCSGG 4 cut(s) 55, 212, 260, 269
AsuHPI GGTGA 5 cut(s) 124, 154, 463, 812, 1019
AvaII GGWCC 2 cut(s) 214, 423
BaeGI GKGCMC 2 cut(s) 238, 713
BalI TGGCCA 1 cut(s) 788
BanII GRGCYC 4 cut(s) 238, 300, 342, 570
BarI GAAGNNNNNNTAC 2 cut(s) 1134, 1166
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 337, 1149
Bbv12I GWGCWC 3 cut(s) 342, 570, 713
BbvI GCAGC 1 cut(s) 453
BccI CCATC 6 cut(s) 161, 194, 326, 722, 823, 954
BceAI ACGGC 5 cut(s) 28, 49, 76, 158, 464
BcgI CGANNNNNNTGC 2 cut(s) 430, 464
BciT130I CCWGG 2 cut(s) 395, 533
BcnI CCSGG 4 cut(s) 55, 212, 260, 269
BcoDI GTCTC 2 cut(s) 649, 789
BfaI CTAG 2 cut(s) 980, 1154
BglII AGATCT 1 cut(s) 228
Bme1390I CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
Bme18I GGWCC 2 cut(s) 214, 423
BmgT120I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
BmiI GGNNCC 2 cut(s) 236, 923
BmrFI CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
BmsI GCATC 2 cut(s) 1056, 1193
BpiI GAAGAC 2 cut(s) 337, 1149
BpmI CTGGAG 2 cut(s) 203, 653
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 4 cut(s) 55, 212, 260, 269
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 1 cut(s) 837
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 5 cut(s) 193, 393, 532, 685, 1218
BsaXI ACNNNNNCTCC 6 cut(s) 149, 179, 230, 260, 374, 404
Bsc4I CCNNNNNNNGG 7 cut(s) 126, 376, 409, 691, 769, 931, 1154
Bse118I RCCGGY 2 cut(s) 420, 484
Bse1I ACTGG 1 cut(s) 1063
Bse3DI GCAATG 1 cut(s) 1212
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 2 cut(s) 395, 533
BseDI CCNNGG 5 cut(s) 193, 393, 532, 685, 1218
BseGI GGATG 3 cut(s) 318, 838, 946
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 7 cut(s) 126, 376, 409, 691, 769, 931, 1154
BseMI GCAATG 1 cut(s) 1212
BseMII CTCAG 1 cut(s) 1215
BseNI ACTGG 1 cut(s) 1063
BseRI GAGGAG 4 cut(s) 106, 275, 881, 1247
BseSI GKGCMC 2 cut(s) 238, 713
BseXI GCAGC 1 cut(s) 453
Bsh1236I CGCG 5 cut(s) 72, 195, 327, 386, 518
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 7 cut(s) 42, 236, 272, 380, 398, 434, 788
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 3 cut(s) 342, 570, 713
BsiSI CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
BslI CCNNNNNNNGG 7 cut(s) 126, 376, 409, 691, 769, 931, 1154
BsmAI GTCTC 2 cut(s) 649, 789
BsmBI CGTCTC 1 cut(s) 789
BsmI GAATGC 1 cut(s) 992
BsnI GGCC 7 cut(s) 42, 236, 272, 380, 398, 434, 788
Bsp120I GGGCCC 1 cut(s) 234
Bsp1286I GDGCHC 5 cut(s) 238, 300, 342, 570, 713
Bsp143I GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
Bsp19I CCATGG 1 cut(s) 685
BspANI GGCC 7 cut(s) 42, 236, 272, 380, 398, 434, 788
BspCNI CTCAG 1 cut(s) 1216
BspFNI CGCG 5 cut(s) 72, 195, 327, 386, 518
BspLI GGNNCC 2 cut(s) 236, 923
BspPI GGATC 2 cut(s) 1180, 1249
BsrBI CCGCTC 1 cut(s) 582
BsrDI GCAATG 1 cut(s) 1212
BsrFI RCCGGY 2 cut(s) 420, 484
BsrI ACTGG 1 cut(s) 1063
BssAI RCCGGY 2 cut(s) 420, 484
BssECI CCNNGG 5 cut(s) 193, 393, 532, 685, 1218
BssMI GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
BssNI GRCGYC 1 cut(s) 15
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 2 cut(s) 685, 1218
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 395, 533
Bst4CI ACNGT 2 cut(s) 800, 1098
Bst6I CTCTTC 5 cut(s) 248, 308, 515, 762, 1253
BstACI GRCGYC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 68, 415
BstDEI CTNAG 1 cut(s) 1224
BstDSI CCRYGG 2 cut(s) 193, 685
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 3 cut(s) 318, 838, 946
BstFNI CGCG 5 cut(s) 72, 195, 327, 386, 518
BstHHI GCGC 3 cut(s) 72, 329, 388
BstKTI GATC 6 cut(s) 231, 507, 942, 1110, 1175, 1244
BstMAI GTCTC 2 cut(s) 649, 789
BstMBI GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 69, 105, 980
BstNI CCWGG 2 cut(s) 395, 533
BstSCI CCNGG 6 cut(s) 53, 210, 258, 267, 393, 531
BstSLI GKGCMC 2 cut(s) 238, 713
BstUI CGCG 5 cut(s) 72, 195, 327, 386, 518
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 2 cut(s) 337, 1149
BstX2I RGATCY 2 cut(s) 228, 1241
BstXI CCANNNNNNTGG 1 cut(s) 829
BstYI RGATCY 2 cut(s) 228, 1241
BsuRI GGCC 7 cut(s) 42, 236, 272, 380, 398, 434, 788
BtgI CCRYGG 2 cut(s) 193, 685
BtsCI GGATG 3 cut(s) 318, 838, 946
BtsIMutI CAGTG 1 cut(s) 1103
Cac8I GCNNGC 2 cut(s) 68, 415
CfoI GCGC 3 cut(s) 72, 329, 388
Cfr10I RCCGGY 2 cut(s) 420, 484
Cfr13I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
Cfr42I CCGCGG 1 cut(s) 196
CpoI CGGWCCG 1 cut(s) 423
CseI GACGC 2 cut(s) 4, 88
Csp6I GTAC 4 cut(s) 58, 577, 781, 1034
CspI CGGWCCG 1 cut(s) 423
CviAII CATG 3 cut(s) 649, 686, 1011
CviQI GTAC 4 cut(s) 58, 577, 781, 1034
DdeI CTNAG 1 cut(s) 1224
DpnI GATC 6 cut(s) 230, 506, 941, 1109, 1174, 1243
DpnII GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
DraIII CACNNNGTG 1 cut(s) 481
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 1 cut(s) 786
Eam1104I CTCTTC 5 cut(s) 248, 308, 515, 762, 1253
EarI CTCTTC 5 cut(s) 248, 308, 515, 762, 1253
EciI GGCGGA 3 cut(s) 157, 167, 1077
Ecl136II GAGCTC 2 cut(s) 340, 568
Eco130I CCWWGG 2 cut(s) 685, 1218
Eco24I GRGCYC 4 cut(s) 238, 300, 342, 570
Eco47I GGWCC 2 cut(s) 214, 423
Eco53kI GAGCTC 2 cut(s) 340, 568
EcoICRI GAGCTC 2 cut(s) 340, 568
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 899
EcoRII CCWGG 2 cut(s) 393, 531
EcoT14I CCWWGG 2 cut(s) 685, 1218
EcoT22I ATGCAT 1 cut(s) 992
EcoT38I GRGCYC 4 cut(s) 238, 300, 342, 570
ErhI CCWWGG 2 cut(s) 685, 1218
Esp3I CGTCTC 1 cut(s) 789
FaeI CATG 3 cut(s) 652, 689, 1014
FalI AAGNNNNNCTT 5 cut(s) 623, 655, 767, 799, 1250
FatI CATG 3 cut(s) 648, 685, 1010
FauI CCCGC 1 cut(s) 85
FblI GTMKAC 1 cut(s) 102
FokI GGATG 3 cut(s) 305, 845, 933
FriOI GRGCYC 4 cut(s) 238, 300, 342, 570
FspBI CTAG 2 cut(s) 980, 1154
GlaI GCGC 3 cut(s) 71, 328, 387
GsuI CTGGAG 2 cut(s) 203, 653
HaeIII GGCC 7 cut(s) 42, 236, 272, 380, 398, 434, 788
HapII CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
HgaI GACGC 2 cut(s) 4, 88
HhaI GCGC 3 cut(s) 72, 329, 388
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 3 cut(s) 652, 689, 1014
Hin6I GCGC 3 cut(s) 70, 327, 386
HinP1I GCGC 3 cut(s) 70, 327, 386
HincII GTYRAC 2 cut(s) 265, 549
HindII GTYRAC 2 cut(s) 265, 549
HinfI GANTC 6 cut(s) 462, 658, 803, 838, 967, 1263
HpaII CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
HphI GGTGA 5 cut(s) 124, 154, 463, 812, 1019
Hpy166II GTNNAC 6 cut(s) 103, 265, 471, 549, 711, 856
Hpy188I TCNGA 2 cut(s) 504, 1257
Hpy188III TCNNGA 6 cut(s) 26, 493, 662, 670, 812, 1193
Hpy8I GTNNAC 6 cut(s) 103, 265, 471, 549, 711, 856
Hpy99I CGWCG 4 cut(s) 20, 338, 452, 455
HpyAV CCTTC 3 cut(s) 147, 324, 619
HpyCH4III ACNGT 2 cut(s) 800, 1098
HpyCH4IV ACGT 2 cut(s) 375, 605
HpyCH4V TGCA 6 cut(s) 4, 441, 677, 711, 990, 1103
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 105, 980
HpyF3I CTNAG 1 cut(s) 1224
HpySE526I ACGT 2 cut(s) 375, 605
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 3 cut(s) 652, 689, 1014
HspAI GCGC 3 cut(s) 70, 327, 386
KspI CCGCGG 1 cut(s) 196
Kzo9I GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
LmnI GCTCC 3 cut(s) 565, 868, 1234
Lsp1109I GCAGC 1 cut(s) 453
LweI GCATC 2 cut(s) 1056, 1193
MaeI CTAG 2 cut(s) 980, 1154
MaeII ACGT 2 cut(s) 375, 605
MaeIII GTNAC 5 cut(s) 655, 662, 733, 817, 1213
MalI GATC 6 cut(s) 230, 506, 941, 1109, 1174, 1243
MbiI CCGCTC 1 cut(s) 582
MboI GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
MflI RGATCY 2 cut(s) 228, 1241
MhlI GDGCHC 5 cut(s) 238, 300, 342, 570, 713
MlsI TGGCCA 1 cut(s) 788
MluCI AATT 3 cut(s) 525, 553, 899
MluNI TGGCCA 1 cut(s) 788
MlyI GAGTC 1 cut(s) 652
MmeI TCCRAC 3 cut(s) 346, 843, 1235
Mox20I TGGCCA 1 cut(s) 788
Mph1103I ATGCAT 1 cut(s) 992
MroXI GAANNNNTTC 3 cut(s) 606, 899, 1262
MscI TGGCCA 1 cut(s) 788
MseI TTAA 4 cut(s) 429, 756, 1055, 1270
MslI CAYNNNNRTG 2 cut(s) 653, 716
Msp20I TGGCCA 1 cut(s) 788
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
MspR9I CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
Mva1269I GAATGC 1 cut(s) 992
MvaI CCWGG 2 cut(s) 395, 533
MvnI CGCG 5 cut(s) 72, 195, 327, 386, 518
MwoI GCNNNNNNNGC 3 cut(s) 69, 105, 980
NciI CCSGG 4 cut(s) 55, 212, 260, 269
NcoI CCATGG 1 cut(s) 685
NdeII GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
NlaIII CATG 3 cut(s) 652, 689, 1014
NlaIV GGNNCC 2 cut(s) 236, 923
NmuCI GTSAC 4 cut(s) 655, 662, 733, 817
NsiI ATGCAT 1 cut(s) 992
OliI CACNNNNGTG 1 cut(s) 716
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 1 cut(s) 992
PdmI GAANNNNTTC 3 cut(s) 606, 899, 1262
PfeI GAWTC 5 cut(s) 462, 803, 838, 967, 1263
PflMI CCANNNNNTGG 1 cut(s) 126
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 376
PsiI TTATAA 1 cut(s) 1130
Psp124BI GAGCTC 2 cut(s) 342, 570
Psp1406I AACGTT 1 cut(s) 605
Psp6I CCWGG 2 cut(s) 393, 531
PspGI CCWGG 2 cut(s) 393, 531
PspN4I GGNNCC 2 cut(s) 236, 923
PspOMI GGGCCC 1 cut(s) 234
PspPI GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
PsuI RGATCY 2 cut(s) 228, 1241
RsaI GTAC 4 cut(s) 59, 578, 782, 1035
RsaNI GTAC 4 cut(s) 58, 577, 781, 1034
RseI CAYNNNNRTG 2 cut(s) 653, 716
Rsr2I CGGWCCG 1 cut(s) 423
RsrII CGGWCCG 1 cut(s) 423
SacI GAGCTC 2 cut(s) 342, 570
SacII CCGCGG 1 cut(s) 196
SaqAI TTAA 4 cut(s) 429, 756, 1055, 1270
Sau3AI GATC 6 cut(s) 228, 504, 939, 1107, 1172, 1241
Sau96I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
SchI GAGTC 1 cut(s) 652
ScrFI CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
SduI GDGCHC 5 cut(s) 238, 300, 342, 570, 713
SfaNI GCATC 2 cut(s) 1056, 1193
Sfr303I CCGCGG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 196
SinI GGWCC 2 cut(s) 214, 423
SmiMI CAYNNNNRTG 2 cut(s) 653, 716
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 3 cut(s) 525, 553, 899
SspMI CTAG 2 cut(s) 980, 1154
SstI GAGCTC 2 cut(s) 342, 570
StyD4I CCNGG 6 cut(s) 53, 210, 258, 267, 393, 531
StyI CCWWGG 2 cut(s) 685, 1218
TaaI ACNGT 2 cut(s) 800, 1098
TaiI ACGT 2 cut(s) 378, 608
TaqI TCGA 9 cut(s) 27, 156, 189, 348, 453, 903, 997, 1081, 1249
TasI AATT 3 cut(s) 525, 553, 899
TatI WGTACW 1 cut(s) 1033
TfiI GAWTC 5 cut(s) 462, 803, 838, 967, 1263
Tru1I TTAA 4 cut(s) 429, 756, 1055, 1270
Tru9I TTAA 4 cut(s) 429, 756, 1055, 1270
TscAI CASTG 1 cut(s) 1110
TseFI GTSAC 4 cut(s) 655, 662, 733, 817
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 4 cut(s) 655, 662, 733, 817
TspDTI ATGAA 6 cut(s) 665, 912, 930, 980, 999, 1056
TspGWI ACGGA 1 cut(s) 415
TspRI CASTG 1 cut(s) 1110
Van91I CCANNNNNTGG 1 cut(s) 126
VneI GTGCAC 1 cut(s) 709
VpaK11BI GGWCC 2 cut(s) 214, 423
XagI CCTNNNNNAGG 1 cut(s) 407
XapI RAATTY 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 3 cut(s) 606, 899, 1262
XspI CTAG 2 cut(s) 980, 1154
Zsp2I ATGCAT 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.