Rh2DG033600

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008018
Physical Location & Seq
Forward (+)
2172516 .. 2174549
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG033600.1

Sequence Viewer

Length: 1287 bp
ATGCACCAAAACGGCGTCGTTGTTCTCGACAATGGCGGCGGCCTAATCAAAGCCGGGTACGGCGGCGAGCGCGACCCCGCCGCCATAATCCCCAACTGCGTCTACCGCCCTCTCTCCTCCAAGAAATGGCTCACCCCTTCCCCCACCGACCCCATCGACCTCACCTCCGCCGCCGTCCGCCGCCCCATCGACCGCGGCTACCTTATCAACCCGGACCTCCAGCGCGAGATCTGGGCCCACCTCTTCTCCCAAGTCCTCCGGGTCAACCCGGCCCAATCCTCCCTCCTCCTGACCGAGCCCCTCTTCACCCTCCCCTCCATCCAACGCGCCGCCGACGAGCTCGTCTTCGAGGACTTCAACTTCGCCGCCCTCTACGTGGCCCACGCGCCGTCGCTGGCCCACCTCTACGAGGCCAGCCGCCGGCCCGTTAAGGCCCAATGCAGCCTCGTCGTCGATTGTGGATTCTCGTTCACCCACGCCGTGCCGGTGTTCCAGAACTTTCCGATCAACTACGCCGCGAAGAGAATTGACCTCGGGGGAAAGGCGTTGACCAATTACCTCAAGGAGCTCGTTTCGTACCGCACTGTGAATGTTATGGACGAAACGTTTCTTATTGACGATGTGAAGGAGAAGCTCTGCTTTGTCTCCATGAATGTGACTCGTGACCTCCAGATTGCAAAGAAGCATGGAAAGGACAATGCTTTCAGGTGCACATATGTGTTGCCTGATGGTGTTACGCATACACAAGGGTATGTTAAAGACCCAGAAGAGGCAAAGAGGTACTTGGCCATGGGAGACAGTGATTCGCTTCAAGAAGTGGCCAAGAAGATGGATGTGAATCGGGTTGAAGTTGTGGACAAGTTGGAGGAGCGAAAGAAGATTGATTTGAGCAAGAATGAATTCGACTTGACAAATGAAAGGTTCCTTGTGCCGGAGATGATCTTCCATCCTGCTGATTTGGGTATGAATCAGGCTGGACTAGCGGAATGCATTGTTCGAGCTGTCAGTTCATGCCATCCTCATCTTCACCCTGTACTTTATGAAAGCATCATTTTAACTGGCGGAAGCACATTGTTTCCTCGACTTGCTGATAGACTAGAGAGAGAGCTGAGGCCTCTTGTTCCTGATGACTATCAAGTGAAGATAACTCCCCAAGAAGACCCCATACTAGGTGTTTGGCGAGGGGGATCAGTTTTAGCATCAAGTCCTGATTTTGAAGCAATGTGTGTTACCAAGGCTGAGTATGAGGAGCTTGGATCTGCTCGATGTCGGAAGAGATTCTTTTAA

Protein Analysis

428

Amino Acids

47.99

Weight (kDa)

6.04

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 3 - 428 1.4e-94 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 126
AccI GTMKAC 1 cut(s) 102
AccII CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
AclI AACGTT 1 cut(s) 605
AclWI GGATC 2 cut(s) 1195, 1264
AcoI YGGCCR 2 cut(s) 786, 819
AcsI RAATTY 1 cut(s) 899
AcyI GRCGYC 1 cut(s) 15
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 4 cut(s) 59, 578, 782, 1035
AfiI CCNNNNNNNGG 7 cut(s) 126, 376, 409, 420, 769, 931, 1169
AgsI TTSAA 4 cut(s) 358, 812, 848, 1217
AleI CACNNNNGTG 1 cut(s) 716
AluBI AGCT 6 cut(s) 340, 568, 634, 1001, 1108, 1252
AluI AGCT 6 cut(s) 340, 568, 634, 1001, 1108, 1252
Alw21I GWGCWC 3 cut(s) 342, 570, 713
Alw26I GTCTC 2 cut(s) 649, 789
Alw44I GTGCAC 1 cut(s) 709
AlwI GGATC 2 cut(s) 1195, 1264
Ama87I CYCGRG 1 cut(s) 533
ApaI GGGCCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 709
ApeKI GCWGC 1 cut(s) 441
ApoI RAATTY 1 cut(s) 899
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
Asp700I GAANNNNTTC 3 cut(s) 606, 899, 1277
AspLEI GCGC 4 cut(s) 72, 225, 329, 388
AspS9I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
AsuC2I CCSGG 4 cut(s) 55, 212, 260, 269
AsuHPI GGTGA 5 cut(s) 124, 154, 298, 463, 1019
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 1 cut(s) 214
BaeGI GKGCMC 2 cut(s) 238, 713
BalI TGGCCA 2 cut(s) 788, 821
BanII GRGCYC 4 cut(s) 238, 300, 342, 570
BarI GAAGNNNNNNTAC 2 cut(s) 1149, 1181
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 337, 1164
Bbv12I GWGCWC 3 cut(s) 342, 570, 713
BbvCI CCTCAGC 1 cut(s) 1109
BbvI GCAGC 1 cut(s) 453
BccI CCATC 7 cut(s) 161, 194, 326, 722, 823, 954, 1023
BceAI ACGGC 5 cut(s) 28, 76, 158, 373, 464
BcgI CGANNNNNNTGC 2 cut(s) 430, 464
BcnI CCSGG 4 cut(s) 55, 212, 260, 269
BcoDI GTCTC 2 cut(s) 649, 789
BfaI CTAG 3 cut(s) 980, 1097, 1169
BglII AGATCT 1 cut(s) 228
Bme1390I CCNGG 4 cut(s) 55, 212, 260, 269
Bme18I GGWCC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
BmiI GGNNCC 2 cut(s) 236, 923
BmrFI CCNGG 4 cut(s) 55, 212, 260, 269
BmsI GCATC 2 cut(s) 1056, 1208
BpiI GAAGAC 2 cut(s) 337, 1164
BpmI CTGGAG 2 cut(s) 203, 653
Bpu10I CCTNAGC 1 cut(s) 1109
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 4 cut(s) 55, 212, 260, 269
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 2 cut(s) 837, 1131
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 4 cut(s) 193, 532, 789, 1233
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 230, 260
Bsc4I CCNNNNNNNGG 7 cut(s) 126, 376, 409, 420, 769, 931, 1169
Bse118I RCCGGY 2 cut(s) 420, 484
Bse1I ACTGG 1 cut(s) 1063
Bse3DI GCAATG 1 cut(s) 1227
Bse8I GATNNNNATC 2 cut(s) 837, 1131
BseDI CCNNGG 4 cut(s) 193, 532, 789, 1233
BseGI GGATG 4 cut(s) 318, 838, 946, 1015
BseJI GATNNNNATC 2 cut(s) 837, 1131
BseLI CCNNNNNNNGG 7 cut(s) 126, 376, 409, 420, 769, 931, 1169
BseMI GCAATG 1 cut(s) 1227
BseMII CTCAG 2 cut(s) 1100, 1230
BseNI ACTGG 1 cut(s) 1063
BseRI GAGGAG 4 cut(s) 106, 275, 881, 1262
BseSI GKGCMC 2 cut(s) 238, 713
BseXI GCAGC 1 cut(s) 453
Bsh1236I CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
Bsh1285I CGRYCG 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 3 cut(s) 342, 570, 713
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
BslI CCNNNNNNNGG 7 cut(s) 126, 376, 409, 420, 769, 931, 1169
BsmAI GTCTC 2 cut(s) 649, 789
BsmI GAATGC 1 cut(s) 992
BsoBI CYCGRG 1 cut(s) 533
Bsp120I GGGCCC 1 cut(s) 234
Bsp1286I GDGCHC 5 cut(s) 238, 300, 342, 570, 713
Bsp143I GATC 5 cut(s) 228, 504, 939, 1187, 1256
Bsp19I CCATGG 1 cut(s) 789
BspCNI CTCAG 2 cut(s) 1101, 1231
BspFNI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
BspLI GGNNCC 2 cut(s) 236, 923
BspPI GGATC 2 cut(s) 1195, 1264
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 2 cut(s) 420, 484
BsrI ACTGG 1 cut(s) 1063
BssAI RCCGGY 2 cut(s) 420, 484
BssECI CCNNGG 4 cut(s) 193, 532, 789, 1233
BssMI GATC 5 cut(s) 228, 504, 939, 1187, 1256
BssNI GRCGYC 1 cut(s) 15
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 2 cut(s) 789, 1233
Bst2BI CACGAG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 586, 800
Bst6I CTCTTC 5 cut(s) 248, 308, 515, 762, 1268
BstACI GRCGYC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 4 cut(s) 68, 396, 415, 422
BstDEI CTNAG 2 cut(s) 1109, 1239
BstDSI CCRYGG 2 cut(s) 193, 789
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 4 cut(s) 318, 838, 946, 1015
BstFNI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
BstHHI GCGC 4 cut(s) 72, 225, 329, 388
BstKTI GATC 5 cut(s) 231, 507, 942, 1190, 1259
BstMAI GTCTC 2 cut(s) 649, 789
BstMBI GATC 5 cut(s) 228, 504, 939, 1187, 1256
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 69, 105, 980
BstSCI CCNGG 4 cut(s) 53, 210, 258, 267
BstSLI GKGCMC 2 cut(s) 238, 713
BstUI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 2 cut(s) 337, 1164
BstX2I RGATCY 2 cut(s) 228, 1256
BstXI CCANNNNNNTGG 1 cut(s) 829
BstYI RGATCY 2 cut(s) 228, 1256
BtgI CCRYGG 2 cut(s) 193, 789
BtsCI GGATG 4 cut(s) 318, 838, 946, 1015
BtsIMutI CAGTG 2 cut(s) 582, 805
Cac8I GCNNGC 4 cut(s) 68, 396, 415, 422
CfoI GCGC 4 cut(s) 72, 225, 329, 388
Cfr10I RCCGGY 2 cut(s) 420, 484
Cfr13I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
Cfr42I CCGCGG 1 cut(s) 196
CseI GACGC 2 cut(s) 4, 88
Csp6I GTAC 4 cut(s) 58, 577, 781, 1034
CviAII CATG 4 cut(s) 649, 686, 790, 1011
CviQI GTAC 4 cut(s) 58, 577, 781, 1034
DdeI CTNAG 2 cut(s) 1109, 1239
DpnI GATC 5 cut(s) 230, 506, 941, 1189, 1258
DpnII GATC 5 cut(s) 228, 504, 939, 1187, 1256
DraIII CACNNNGTG 1 cut(s) 481
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 2 cut(s) 786, 819
Eam1104I CTCTTC 5 cut(s) 248, 308, 515, 762, 1268
EarI CTCTTC 5 cut(s) 248, 308, 515, 762, 1268
EciI GGCGGA 3 cut(s) 157, 167, 1077
Ecl136II GAGCTC 2 cut(s) 340, 568
Eco130I CCWWGG 2 cut(s) 789, 1233
Eco147I AGGCCT 1 cut(s) 1114
Eco24I GRGCYC 4 cut(s) 238, 300, 342, 570
Eco47I GGWCC 1 cut(s) 214
Eco53kI GAGCTC 2 cut(s) 340, 568
Eco88I CYCGRG 1 cut(s) 533
EcoICRI GAGCTC 2 cut(s) 340, 568
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 899
EcoT14I CCWWGG 2 cut(s) 789, 1233
EcoT22I ATGCAT 1 cut(s) 992
EcoT38I GRGCYC 4 cut(s) 238, 300, 342, 570
ErhI CCWWGG 2 cut(s) 789, 1233
FaeI CATG 4 cut(s) 652, 689, 793, 1014
FalI AAGNNNNNCTT 5 cut(s) 623, 655, 767, 799, 1265
FatI CATG 4 cut(s) 648, 685, 789, 1010
FauI CCCGC 1 cut(s) 85
FauNDI CATATG 1 cut(s) 715
FblI GTMKAC 1 cut(s) 102
FokI GGATG 4 cut(s) 305, 845, 933, 1002
FriOI GRGCYC 4 cut(s) 238, 300, 342, 570
FspBI CTAG 3 cut(s) 980, 1097, 1169
GlaI GCGC 4 cut(s) 71, 224, 328, 387
GsuI CTGGAG 2 cut(s) 203, 653
HapII CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
HgaI GACGC 2 cut(s) 4, 88
HhaI GCGC 4 cut(s) 72, 225, 329, 388
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 4 cut(s) 652, 689, 793, 1014
Hin6I GCGC 4 cut(s) 70, 223, 327, 386
HinP1I GCGC 4 cut(s) 70, 223, 327, 386
HincII GTYRAC 2 cut(s) 265, 549
HindII GTYRAC 2 cut(s) 265, 549
HinfI GANTC 6 cut(s) 462, 658, 803, 838, 967, 1278
HpaII CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
HphI GGTGA 5 cut(s) 124, 154, 298, 463, 1019
Hpy166II GTNNAC 6 cut(s) 103, 265, 471, 549, 711, 856
Hpy188I TCNGA 2 cut(s) 504, 1272
Hpy188III TCNNGA 8 cut(s) 26, 289, 493, 662, 670, 812, 1124, 1208
Hpy8I GTNNAC 6 cut(s) 103, 265, 471, 549, 711, 856
Hpy99I CGWCG 5 cut(s) 20, 338, 394, 452, 455
HpyAV CCTTC 2 cut(s) 147, 619
HpyCH4III ACNGT 2 cut(s) 586, 800
HpyCH4IV ACGT 2 cut(s) 375, 605
HpyCH4V TGCA 5 cut(s) 4, 441, 677, 711, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 105, 980
HpyF3I CTNAG 2 cut(s) 1109, 1239
HpySE526I ACGT 2 cut(s) 375, 605
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 4 cut(s) 652, 689, 793, 1014
HspAI GCGC 4 cut(s) 70, 223, 327, 386
KroI GCCGGC 1 cut(s) 420
KroNI GCCGGC 1 cut(s) 422
KspI CCGCGG 1 cut(s) 196
Kzo9I GATC 5 cut(s) 228, 504, 939, 1187, 1256
LmnI GCTCC 3 cut(s) 565, 868, 1249
Lsp1109I GCAGC 1 cut(s) 453
LweI GCATC 2 cut(s) 1056, 1208
MaeI CTAG 3 cut(s) 980, 1097, 1169
MaeII ACGT 2 cut(s) 375, 605
MaeIII GTNAC 4 cut(s) 655, 662, 733, 1228
MalI GATC 5 cut(s) 230, 506, 941, 1189, 1258
MboI GATC 5 cut(s) 228, 504, 939, 1187, 1256
MflI RGATCY 2 cut(s) 228, 1256
MhlI GDGCHC 5 cut(s) 238, 300, 342, 570, 713
MlsI TGGCCA 2 cut(s) 788, 821
MluCI AATT 3 cut(s) 525, 553, 899
MluNI TGGCCA 2 cut(s) 788, 821
MlyI GAGTC 1 cut(s) 652
MmeI TCCRAC 3 cut(s) 346, 843, 1250
Mox20I TGGCCA 2 cut(s) 788, 821
Mph1103I ATGCAT 1 cut(s) 992
MroNI GCCGGC 1 cut(s) 420
MroXI GAANNNNTTC 3 cut(s) 606, 899, 1277
MscI TGGCCA 2 cut(s) 788, 821
MseI TTAA 4 cut(s) 429, 756, 1055, 1285
MslI CAYNNNNRTG 2 cut(s) 653, 716
Msp20I TGGCCA 2 cut(s) 788, 821
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
MspR9I CCNGG 4 cut(s) 55, 212, 260, 269
Mva1269I GAATGC 1 cut(s) 992
MvnI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
MwoI GCNNNNNNNGC 3 cut(s) 69, 105, 980
NaeI GCCGGC 1 cut(s) 422
NciI CCSGG 4 cut(s) 55, 212, 260, 269
NcoI CCATGG 1 cut(s) 789
NdeI CATATG 1 cut(s) 715
NdeII GATC 5 cut(s) 228, 504, 939, 1187, 1256
NgoMIV GCCGGC 1 cut(s) 420
NlaIII CATG 4 cut(s) 652, 689, 793, 1014
NlaIV GGNNCC 2 cut(s) 236, 923
NmuCI GTSAC 2 cut(s) 655, 662
NsiI ATGCAT 1 cut(s) 992
OliI CACNNNNGTG 1 cut(s) 716
PceI AGGCCT 1 cut(s) 1114
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 1 cut(s) 992
PdiI GCCGGC 1 cut(s) 422
PdmI GAANNNNTTC 3 cut(s) 606, 899, 1277
PfeI GAWTC 5 cut(s) 462, 803, 838, 967, 1278
PflMI CCANNNNNTGG 1 cut(s) 126
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 376
Psp124BI GAGCTC 2 cut(s) 342, 570
Psp1406I AACGTT 1 cut(s) 605
PspN4I GGNNCC 2 cut(s) 236, 923
PspOMI GGGCCC 1 cut(s) 234
PspPI GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
PsuI RGATCY 2 cut(s) 228, 1256
RsaI GTAC 4 cut(s) 59, 578, 782, 1035
RsaNI GTAC 4 cut(s) 58, 577, 781, 1034
RseI CAYNNNNRTG 2 cut(s) 653, 716
SacI GAGCTC 2 cut(s) 342, 570
SacII CCGCGG 1 cut(s) 196
SaqAI TTAA 4 cut(s) 429, 756, 1055, 1285
Sau3AI GATC 5 cut(s) 228, 504, 939, 1187, 1256
Sau96I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
SchI GAGTC 1 cut(s) 652
ScrFI CCNGG 4 cut(s) 55, 212, 260, 269
SduI GDGCHC 5 cut(s) 238, 300, 342, 570, 713
SfaNI GCATC 2 cut(s) 1056, 1208
Sfr303I CCGCGG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 196
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 2 cut(s) 653, 716
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 3 cut(s) 525, 553, 899
SseBI AGGCCT 1 cut(s) 1114
SspMI CTAG 3 cut(s) 980, 1097, 1169
SstI GAGCTC 2 cut(s) 342, 570
StuI AGGCCT 1 cut(s) 1114
StyD4I CCNGG 4 cut(s) 53, 210, 258, 267
StyI CCWWGG 2 cut(s) 789, 1233
TaaI ACNGT 2 cut(s) 586, 800
TaiI ACGT 2 cut(s) 378, 608
TaqI TCGA 9 cut(s) 27, 156, 189, 348, 453, 903, 997, 1081, 1264
TaqII GACCGA 1 cut(s) 308
TasI AATT 3 cut(s) 525, 553, 899
TatI WGTACW 1 cut(s) 1033
TfiI GAWTC 5 cut(s) 462, 803, 838, 967, 1278
Tru1I TTAA 4 cut(s) 429, 756, 1055, 1285
Tru9I TTAA 4 cut(s) 429, 756, 1055, 1285
TscAI CASTG 2 cut(s) 589, 805
TseFI GTSAC 2 cut(s) 655, 662
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 2 cut(s) 655, 662
TspDTI ATGAA 6 cut(s) 665, 912, 930, 980, 999, 1056
TspRI CASTG 2 cut(s) 589, 805
Van91I CCANNNNNTGG 1 cut(s) 126
VneI GTGCAC 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 214
XagI CCTNNNNNAGG 1 cut(s) 407
XapI RAATTY 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 3 cut(s) 606, 899, 1277
XspI CTAG 3 cut(s) 980, 1097, 1169
Zsp2I ATGCAT 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.