RLG00000016038

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017553
Physical Location & Seq
Reverse (-)
3360994 .. 3363384
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016038

Sequence Viewer

Length: 1668 bp
ATGCTCCTTCTTGGACTACACCAATGCAGAGCCACCCTCAACAAGCTTATGGTCTTCTGCACAGACCCAACTCTTGGAGACTTGCATTACGCCGAAAAAATCTTCGATCACATCCAAACCCCTGGTTTGTTCATCTACAATGTAATGATCAAAGCTTTAGCCAAAAGGAGAAGATTCAAAAGCGTCATTGAGCTCTTTCGGTTGTTGAGGGAAGAGGGTTTGTGGCCCGATAGTTTCACGTACCCATTTGCTTGTAAAGCCATTGGAGGCTTGGGAGAGGCTCACGAAGGCGCAAAGGTTCATGCGTTTGCGGTAAAGACCGGACTTCAGTTCGATTCGTATGTGTGTAACTCACTCATTGACATGTATGCGGAATTGGGTCTAGTTGATAATGTGAAGCAGGTGTTTGATGAGATGCCTGAGAGAGATGTTGTTTCTTGGAATGTTACGATTTCAGGGTGTGTTAGGTGTATGAGGTTTAGGGAGGCAGCGAGTGTGTTCCGGCGGATGAGAAAAGAGAGCAATGAGAAGCCGGATGAATCTACTGTTGTGAGCACGCTTTCGGCTTGCGCAGCACTGAAAGATGTGGAGCTTGGTAGAGAAATTCATGACTATGTTAGAGCTGAGCTTATGTTCACTAGTATAATCGGAAATGCGGTGTTGGATATGTATGCTAAGTGTGGTTGTTTGAGTGAAGCAAGGAAAGTTTTCGATGAAACTCCGTTGAAAAATGTCATGTGTTGGACTAGTATGGTGTGTGGGTATGTGAATTGTGGTATGCTGGATGAGGCTAGAGAGTTGTTTGATAAGAGTCCTACTAAGGATGTTGTTCTATGGACAGCAATGATGAATGGGTATGTACAATATAACCAATTTGATGAGGCAGTGGCTCTATTCCAAGAGATGCAGTTTAGAAGAGTTCAAGTGGATAAATTCACAGTTGTTGCGCTCCTCACAGGCTGTGCTCAGTTGGGAGCATTAGAGCAAGGGAAATGGATTCACGGATACATAGACGAAAATGGAATCAAAATTGATGCAGTTGTTGGTACTGCTCTTATAGAAATGTATGCAAAATGTGGGTGCATAGACAAGTCTCTGGAGATTTTCAATGCATTGAATGAAAAAGATGCAGCCACTTGGACTTCAATAGTCTGCGCGCTTGCAATGAATGGCAAAACAAGCAAAGCACTCGAGTTGTTCTCCAAAATGAAGCAGGTTAGGGTCAACCCTGATGATATCACCTTCATTGGTGTCTTGAGTGCTTGTAGTCATGGAGGATTGGTTGACGAAGGACGAAAGTTGTTCAACTCCATGAGAAAGGACTATGGGATCGAGCCAAAGCTAGAACATTATGGGTGTCTGATTGACCTTCTTGGTCGGGCTGGACTCCTAGACGAGGCAGAGGAGCTGATAGAAAGTATTCCCAGTGAAAACAATAAGATTTTAGTTCCACTTTACAGTTCTTTACTCAGTGCTTGCAGAATCCATGGCAATGTTGACATGAGCGAAAGAGTGGCTAAAAGGCTATCGGATATTGAGTCCAGCGGCTCGAGTGCTCACACGCTTCTGGCCAACATCTACGCAGATGCTGAGAGATGGGAAGATGTAACAAAACGGGAGCGAAATCGTAATCTGCATCATCATCATCGTCCCCGTCCCCTTCAGTGA

Protein Analysis

556

Amino Acids

62.44

Weight (kDa)

5.98

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 41 - 81 1.2e-06 PPR repeat family
PPR_2 PF13041 142 - 191 8.3e-07 PPR repeat family
PPR_2 PF13041 274 - 322 2.9e-12 PPR repeat family
PPR PF01535 277 - 305 1.7e-06 PPR repeat
PPR_2 PF13041 376 - 423 6.4e-09 PPR repeat family
E_motif PF20431 496 - 538 3.7e-08 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017553)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31430 AT1G31430
fragaria_vesca FvH4_1g04051
malus_domestica MD04G1011600.v1.1
prunus_persica Prupe.7G236400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0089541
rosa_laevigata RLG00000016038
rosa_multiflora Rmu_sc0006193.1_g000007
rosa_rugosa Rorug02G0001500
rosa_samantha Rh2BG045500 Rh2BG045600 Rh2DG046800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc16I TGCGCA 1 cut(s) 571
Acc36I ACCTGC 2 cut(s) 391, 1204
AccB7I CCANNNNNTGG 1 cut(s) 74
AccII CGCG 1 cut(s) 1157
AciI CCGC 5 cut(s) 311, 371, 505, 656, 1545
AclWI GGATC 1 cut(s) 1337
AcoI YGGCCR 1 cut(s) 1569
AcsI RAATTY 2 cut(s) 603, 932
AcuI CTGAAG 2 cut(s) 311, 1646
AfaI GTAC 3 cut(s) 242, 861, 1048
AfiI CCNNNNNNNGG 2 cut(s) 74, 1396
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 7 cut(s) 178, 727, 923, 1108, 1117, 1146, 1306
AhlI ACTAGT 2 cut(s) 638, 746
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 8 cut(s) 46, 155, 193, 592, 623, 628, 1342, 1408
AluI AGCT 8 cut(s) 46, 155, 193, 592, 623, 628, 1342, 1408
Alw21I GWGCWC 4 cut(s) 195, 557, 967, 1558
Alw26I GTCTC 2 cut(s) 72, 1098
AlwI GGATC 1 cut(s) 1337
AlwNI CAGNNNCTG 1 cut(s) 1589
Ama87I CYCGRG 2 cut(s) 1190, 1549
AoxI GGCC 2 cut(s) 224, 1569
ApeKI GCWGC 3 cut(s) 488, 572, 1130
ApoI RAATTY 2 cut(s) 603, 932
Asp700I GAANNNNTTC 2 cut(s) 707, 1419
AspLEI GCGC 5 cut(s) 293, 572, 949, 1157, 1159
AspS9I GGNCC 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 1231
AvaI CYCGRG 2 cut(s) 1190, 1549
BalI TGGCCA 1 cut(s) 1571
BanII GRGCYC 1 cut(s) 195
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 4 cut(s) 195, 557, 967, 1558
BbvI GCAGC 3 cut(s) 500, 584, 1142
BccI CCATC 1 cut(s) 1590
BcgI CGANNNNNNTGC 2 cut(s) 1171, 1205
BciT130I CCWGG 1 cut(s) 123
BciVI GTATCC 1 cut(s) 998
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 72, 1098
BcuI ACTAGT 2 cut(s) 638, 746
BfaI CTAG 6 cut(s) 383, 639, 747, 792, 1343, 1391
BfuAI ACCTGC 2 cut(s) 391, 1204
BfuI GTATCC 1 cut(s) 998
BisI GCNGC 4 cut(s) 489, 573, 1131, 1546
BlpI GCTNAGC 1 cut(s) 624
BlsI GCNGC 4 cut(s) 490, 574, 1132, 1547
Bme1390I CCNGG 1 cut(s) 123
BmeT110I CYCGRG 2 cut(s) 1190, 1549
BmgT120I GGNCC 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 1419
BmsI GCATC 6 cut(s) 405, 894, 1024, 1117, 1576, 1645
BmuI ACTGGG 1 cut(s) 1419
BpiI GAAGAC 1 cut(s) 46
BplI GAGNNNNNCTC 4 cut(s) 21, 53, 1184, 1216
BpmI CTGGAG 1 cut(s) 1118
Bpu1102I GCTNAGC 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 1276
BsaAI YACGTR 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 121, 1486
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 2 cut(s) 1266, 1296
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 1396
Bse1I ACTGG 1 cut(s) 1425
Bse3DI GCAATG 4 cut(s) 529, 849, 1170, 1498
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 121, 1486
BseGI GGATG 5 cut(s) 111, 513, 541, 790, 829
BseLI CCNNNNNNNGG 2 cut(s) 74, 1396
BseMI GCAATG 4 cut(s) 529, 849, 1170, 1498
BseMII CTCAG 5 cut(s) 411, 615, 980, 1483, 1581
BseNI ACTGG 1 cut(s) 1425
BsePI GCGCGC 1 cut(s) 1155
BseRI GAGGAG 2 cut(s) 941, 1418
BseXI GCAGC 3 cut(s) 500, 584, 1142
BsgI GTGCAG 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 1157
BshFI GGCC 2 cut(s) 226, 1571
BsiHKAI GWGCWC 4 cut(s) 195, 557, 967, 1558
BsiHKCI CYCGRG 2 cut(s) 1190, 1549
BsiSI CCGG 3 cut(s) 321, 502, 533
BslFI GGGAC 2 cut(s) 1635, 1641
BslI CCNNNNNNNGG 2 cut(s) 74, 1396
BsmAI GTCTC 2 cut(s) 72, 1098
BsmFI GGGAC 2 cut(s) 1635, 1641
BsnI GGCC 2 cut(s) 226, 1571
BsoBI CYCGRG 2 cut(s) 1190, 1549
Bsp1286I GDGCHC 4 cut(s) 195, 557, 967, 1558
Bsp1407I TGTACA 1 cut(s) 859
Bsp143I GATC 3 cut(s) 106, 147, 1329
Bsp1720I GCTNAGC 1 cut(s) 624
Bsp19I CCATGG 1 cut(s) 1486
BspACI CCGC 5 cut(s) 311, 371, 505, 656, 1545
BspANI GGCC 2 cut(s) 226, 1571
BspCNI CTCAG 5 cut(s) 412, 616, 979, 1482, 1582
BspFNI CGCG 1 cut(s) 1157
BspHI TCATGA 1 cut(s) 607
BspMI ACCTGC 2 cut(s) 391, 1204
BspPI GGATC 1 cut(s) 1337
BsrDI GCAATG 4 cut(s) 529, 849, 1170, 1498
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 1 cut(s) 1425
BssECI CCNNGG 2 cut(s) 121, 1486
BssHII GCGCGC 1 cut(s) 1155
BssMI GATC 3 cut(s) 106, 147, 1329
BssT1I CCWWGG 1 cut(s) 1486
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 3 cut(s) 547, 940, 1460
Bst6I CTCTTC 2 cut(s) 207, 910
BstAUI TGTACA 1 cut(s) 859
BstBAI YACGTR 1 cut(s) 240
BstC8I GCNNGC 5 cut(s) 557, 568, 1157, 1161, 1477
BstDEI CTNAG 7 cut(s) 420, 624, 675, 819, 966, 1469, 1590
BstDSI CCRYGG 1 cut(s) 1486
BstENI CCTNNNNNAGG 1 cut(s) 1394
BstF5I GGATG 5 cut(s) 111, 513, 541, 790, 829
BstFNI CGCG 1 cut(s) 1157
BstHHI GCGC 5 cut(s) 293, 572, 949, 1157, 1159
BstKTI GATC 3 cut(s) 109, 150, 1332
BstMAI GTCTC 2 cut(s) 72, 1098
BstMBI GATC 3 cut(s) 106, 147, 1329
BstMWI GCNNNNNNNGC 3 cut(s) 257, 572, 1179
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 121
BstUI CGCG 1 cut(s) 1157
BstV1I GCAGC 3 cut(s) 500, 584, 1142
BstV2I GAAGAC 1 cut(s) 46
BstXI CCANNNNNNTGG 1 cut(s) 122
BsuI GTATCC 1 cut(s) 998
BsuRI GGCC 2 cut(s) 226, 1571
BtgI CCRYGG 1 cut(s) 1486
BtsCI GGATG 5 cut(s) 111, 513, 541, 790, 829
BtsI GCAGTG 1 cut(s) 891
BtsIMutI CAGTG 4 cut(s) 575, 891, 1432, 1477
BveI ACCTGC 2 cut(s) 391, 1204
Cac8I GCNNGC 5 cut(s) 557, 568, 1157, 1161, 1477
CaiI CAGNNNCTG 1 cut(s) 1589
CciI TCATGA 1 cut(s) 607
CfoI GCGC 5 cut(s) 293, 572, 949, 1157, 1159
Cfr13I GGNCC 1 cut(s) 225
CseI GACGC 1 cut(s) 172
Csp6I GTAC 3 cut(s) 241, 860, 1047
CviAII CATG 8 cut(s) 302, 364, 608, 736, 1271, 1312, 1487, 1501
CviQI GTAC 3 cut(s) 241, 860, 1047
DdeI CTNAG 7 cut(s) 420, 624, 675, 819, 966, 1469, 1590
DpnI GATC 3 cut(s) 108, 149, 1331
DpnII GATC 3 cut(s) 106, 147, 1329
EaeI YGGCCR 1 cut(s) 1569
Eam1104I CTCTTC 2 cut(s) 207, 910
EarI CTCTTC 2 cut(s) 207, 910
EciI GGCGGA 1 cut(s) 520
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 1486
Eco24I GRGCYC 1 cut(s) 195
Eco32I GATATC 1 cut(s) 1237
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 311, 1646
Eco88I CYCGRG 2 cut(s) 1190, 1549
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 1394
EcoRII CCWGG 1 cut(s) 121
EcoRV GATATC 1 cut(s) 1237
EcoT14I CCWWGG 1 cut(s) 1486
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 1486
FaeI CATG 8 cut(s) 305, 367, 611, 739, 1274, 1315, 1490, 1504
FaqI GGGAC 2 cut(s) 1635, 1641
FatI CATG 8 cut(s) 301, 363, 607, 735, 1270, 1311, 1486, 1500
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 489, 573, 1131, 1546
FokI GGATG 5 cut(s) 98, 520, 548, 797, 836
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 4 cut(s) 489, 573, 1131, 1546
FspBI CTAG 6 cut(s) 383, 639, 747, 792, 1343, 1391
FspI TGCGCA 1 cut(s) 571
GlaI GCGC 5 cut(s) 292, 571, 948, 1156, 1158
GluI GCNGC 4 cut(s) 489, 573, 1131, 1546
GsuI CTGGAG 1 cut(s) 1118
HaeIII GGCC 2 cut(s) 226, 1571
HapII CCGG 3 cut(s) 321, 502, 533
HgaI GACGC 1 cut(s) 172
HhaI GCGC 5 cut(s) 293, 572, 949, 1157, 1159
Hin1II CATG 8 cut(s) 305, 367, 611, 739, 1274, 1315, 1490, 1504
Hin6I GCGC 5 cut(s) 291, 570, 947, 1155, 1157
HinP1I GCGC 5 cut(s) 291, 570, 947, 1155, 1157
HincII GTYRAC 3 cut(s) 1225, 1285, 1498
HindII GTYRAC 3 cut(s) 1225, 1285, 1498
HindIII AAGCTT 2 cut(s) 44, 153
HinfI GANTC 9 cut(s) 174, 335, 539, 811, 997, 1023, 1386, 1482, 1538
HpaII CCGG 3 cut(s) 321, 502, 533
HphI GGTGA 1 cut(s) 1231
Hpy166II GTNNAC 4 cut(s) 636, 1225, 1285, 1498
Hpy188I TCNGA 3 cut(s) 650, 1362, 1531
Hpy188III TCNNGA 4 cut(s) 284, 608, 1097, 1255
Hpy8I GTNNAC 4 cut(s) 636, 1225, 1285, 1498
HpyAV CCTTC 5 cut(s) 17, 281, 1252, 1283, 1379
HpyCH4III ACNGT 3 cut(s) 547, 940, 1460
HpyCH4IV ACGT 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 572, 1179
HpyF3I CTNAG 7 cut(s) 420, 624, 675, 819, 966, 1469, 1590
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 8 cut(s) 305, 367, 611, 739, 1274, 1315, 1490, 1504
HspAI GCGC 5 cut(s) 291, 570, 947, 1155, 1157
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 3 cut(s) 106, 147, 1329
LmnI GCTCC 6 cut(s) 9, 589, 954, 974, 1405, 1618
Lsp1109I GCAGC 3 cut(s) 500, 584, 1142
LweI GCATC 6 cut(s) 405, 894, 1024, 1117, 1576, 1645
MaeI CTAG 6 cut(s) 383, 639, 747, 792, 1343, 1391
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 3 cut(s) 347, 445, 1606
MalI GATC 3 cut(s) 108, 149, 1331
MboI GATC 3 cut(s) 106, 147, 1329
MboII GAAGA 6 cut(s) 46, 94, 183, 224, 927, 1613
MhlI GDGCHC 4 cut(s) 195, 557, 967, 1558
MlsI TGGCCA 1 cut(s) 1571
MluCI AATT 6 cut(s) 374, 603, 769, 872, 932, 1029
MluNI TGGCCA 1 cut(s) 1571
MlyI GAGTC 3 cut(s) 820, 1380, 1547
MmeI TCCRAC 2 cut(s) 642, 722
Mox20I TGGCCA 1 cut(s) 1571
Mph1103I ATGCAT 1 cut(s) 1114
MroXI GAANNNNTTC 2 cut(s) 707, 1419
MscI TGGCCA 1 cut(s) 1571
MslI CAYNNNNRTG 3 cut(s) 362, 612, 1491
Msp20I TGGCCA 1 cut(s) 1571
MspA1I CMGCKG 1 cut(s) 1545
MspI CCGG 3 cut(s) 321, 502, 533
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 1157
MwoI GCNNNNNNNGC 3 cut(s) 257, 572, 1179
NcoI CCATGG 1 cut(s) 1486
NdeII GATC 3 cut(s) 106, 147, 1329
NlaIII CATG 8 cut(s) 305, 367, 611, 739, 1274, 1315, 1490, 1504
NsbI TGCGCA 1 cut(s) 571
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 1 cut(s) 367
PaeR7I CTCGAG 2 cut(s) 1190, 1549
PagI TCATGA 1 cut(s) 607
PaqCI CACCTGC 1 cut(s) 391
PauI GCGCGC 1 cut(s) 1155
PciI ACATGT 1 cut(s) 363
PdmI GAANNNNTTC 2 cut(s) 707, 1419
PfeI GAWTC 6 cut(s) 174, 335, 539, 997, 1023, 1482
PflMI CCANNNNNTGG 1 cut(s) 74
PkrI GCNGC 4 cut(s) 490, 574, 1132, 1547
PleI GAGTC 3 cut(s) 819, 1380, 1546
PpsI GAGTC 3 cut(s) 819, 1380, 1546
Ppu21I YACGTR 1 cut(s) 240
PscI ACATGT 1 cut(s) 363
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspPI GGNCC 1 cut(s) 225
PspXI VCTCGAGB 2 cut(s) 1190, 1549
PstNI CAGNNNCTG 1 cut(s) 1589
PteI GCGCGC 1 cut(s) 1155
RsaI GTAC 3 cut(s) 242, 861, 1048
RsaNI GTAC 3 cut(s) 241, 860, 1047
RseI CAYNNNNRTG 3 cut(s) 362, 612, 1491
SacI GAGCTC 1 cut(s) 195
SatI GCNGC 4 cut(s) 489, 573, 1131, 1546
Sau3AI GATC 3 cut(s) 106, 147, 1329
Sau96I GGNCC 1 cut(s) 225
SchI GAGTC 3 cut(s) 820, 1380, 1547
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 4 cut(s) 195, 557, 967, 1558
SfaNI GCATC 6 cut(s) 405, 894, 1024, 1117, 1576, 1645
Sfr274I CTCGAG 2 cut(s) 1190, 1549
SlaI CTCGAG 2 cut(s) 1190, 1549
SmiMI CAYNNNNRTG 3 cut(s) 362, 612, 1491
SmlI CTYRAG 3 cut(s) 1190, 1255, 1549
SmoI CTYRAG 3 cut(s) 1190, 1255, 1549
SpeI ACTAGT 2 cut(s) 638, 746
Sse9I AATT 6 cut(s) 374, 603, 769, 872, 932, 1029
SsiI CCGC 5 cut(s) 311, 371, 505, 656, 1545
SspMI CTAG 6 cut(s) 383, 639, 747, 792, 1343, 1391
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 1486
TaaI ACNGT 3 cut(s) 547, 940, 1460
TaiI ACGT 1 cut(s) 242
TaqI TCGA 6 cut(s) 105, 333, 711, 1191, 1332, 1550
TasI AATT 6 cut(s) 374, 603, 769, 872, 932, 1029
TatI WGTACW 1 cut(s) 859
TauI GCSGC 1 cut(s) 1548
TfiI GAWTC 6 cut(s) 174, 335, 539, 997, 1023, 1482
TscAI CASTG 4 cut(s) 582, 891, 1432, 1477
TseI GCWGC 3 cut(s) 488, 572, 1130
TspGWI ACGGA 2 cut(s) 711, 1017
TspRI CASTG 4 cut(s) 582, 891, 1432, 1477
Van91I CCANNNNNTGG 1 cut(s) 74
XagI CCTNNNNNAGG 1 cut(s) 1394
XapI RAATTY 2 cut(s) 603, 932
XceI RCATGY 1 cut(s) 367
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XhoI CTCGAG 2 cut(s) 1190, 1549
XmnI GAANNNNTTC 2 cut(s) 707, 1419
XspI CTAG 6 cut(s) 383, 639, 747, 792, 1343, 1391
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.