Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0009041

Physical Location & Seq

Genomic Coordinates Reverse (-)

6913645 .. 6919194

Gene Structure 5550 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016471

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGTCGTTTCTGAGACGGAGAAAGGGGAAGGCGGAGAATTTGGAGCCGGACGAGAGTGAGACAGAGAAGGAACACGAACCCGGTGAGGAGAAGCAAACGACGAAGCGTCGTCGGAGAAGGAACCCGAACCCGAACCGGTGGTCGTGCGTGGCCAACTGCTGCTGGCTCATCGGAGTCATATGCTCGATCTGGTGGTTCCTGCTGTTCTTGCTCAACGCAATGCCGGCGTCGTTTCCTCAGTACGTGACGGAGGCGATAACGGGTCCGGTTTCCGACCCGCCCGGCGTGAAATTGAGGAAGGAGGGTTTGAAGGCGAAGCACCCGGTGGTGTTTGTGCCCGGAATTGTCACCGGCGGGCTCGAATTGTGGGAAGGGCATAGCTGCGCCGATGGGTTGTTTAGGAAGAGATTGTGGGGCGGCACATTTGGGGAATTGTATAAAAGACCATTATGCTGGGTTGAACATATGTCTCTGGACAATGAAACTGGACTGGACCCTGCAGGTATAAGGGTTAGGCCTGTATCTGGTCTTGTGGCAGCTGATTACTTTGCCCCTGGTTACTTTGTGTGGGCAGTTTTAATTGCTAATTTGGCTCGCATTGGATATGAGGAGAAAACCATGTATATGGCTTCATATGATTGGAGATTATCGTTTCAGAATACAGAGGTTAGGGACCAAACTTTGAGCAGAATAAAAAGTAATATAGAACTTATGGTCGCTACAAATGGTGGCAACAAGGTGGTTGTTCTCCCACATTCAATGGGTGTTCTGTACTTTCTGCATTTTATGAAATGGGTTGAGGCACCAGCACCAATGGGAGGTGGGGGTGGATCAGATTGGTGTGCTAAGCATATTAAAGCTGTAATGAACATCGGTGGCCCCTTTTTAGGGGTTCCAAAAGCTGTTGCTGGACTTTTCTCTATTGAGGCCAAAGACATTGCTGTTGCCAGGGCTTTTGCACCAGGTGTTTTGGATAAGGATGTTTTTGGTCTCCAAACCTTACAACATATGATGCGGATGACCCGGACATGGGATTCAACTATGTCTATGATACCAAAAGGCGGGGACACAATATGGGGTGGTCTTGACTGGTCACCTGAAGAATACTGCCATTGTGATGCGAAGAAGTTGAAGAATAATGATACCAAAGATGCAGAGCCATATGGAGTAAATTATGGGAGGATTATATCATTTGGGAAAGACGTAGCGGAGACGCATTCCTCCAAGATTGAGAGGATCGATTTTAGGGATGCTGTTAAGGGTAATTTACTTGCGAATGCTACCCATTGTGATGTATGGACAGAGTACCATGACATGGGTGTTGGGGGTGTCAAAGCTGTTTCAGATTACAAAATCTACACAGCTGGATCAATTTTGGATCTGCTTCATTTTGTTGCGCCCAAATTGATGGCACGTGGGGATGCTCATTTTTCGTATGGAATTGCGGACAATTTGGATGACCCCAAGTATGAACACTACAAATATTGGTCAAACCCCCTAGAAACAAGATTACCAAATGCTCCTGATATGGAGATCTATTCTATGTATGGTGTTGGCATCCCTACTGAAAGAGCATATGTGTATAAGTTAACTCCTACTGCTGAATGCTACATTCCCTTTCAAATAGATACCTCAGCAGAGGGTGGAAGCGACAACCCGTGTTTAAAAGGTGGAGTGTACTCCGCTGATGGGGATGAAACTGTGCCGGTGTTGAGTGCAGGTTTCATGTGTGCTAAAGCCTGGAGAGGAAAGACCCGTTTCAACCCTTCTGGCATTCGTACTAACTTAAGGGAGTACGACCATGCCCCTCCAGCTAATCTTCTAGAGGGCCGGGGTACCCAAAGTGGTGCTCATGTTGATATAATGGGAAACTTTGCTTTAATTGAGGATGTTTTAAGAGTAGCAGCAGGCGCTACGGGAGATGAATTGGGTGGAGATAAGGTGCACTCTGATATTTTCAAATGGTCTGAAAGGATCAATATAGATCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008080 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046027 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 map00561

Pfam Domains

Protein Families

LCAT

Protein Analysis

664

Amino Acids

73.65

Weight (kDa)

6.25

Isoelectric Point (pI)

41.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LCAT	PF02450	133 - 623	1.1e-62	Lecithin:cholesterol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009041)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13640 AT5G13640 AT5G13640
fragaria_vesca	FvH4_1g08180 FvH4_1g08180 FvH4_1g08180 FvH4_1g08180 FvH4_1g08180
malus_domestica	MD02G1086400.v1.1 MD15G1213500.v1.1
prunus_persica	Prupe.7G203400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0094251
rosa_laevigata	RLG00000016471
rosa_multiflora	Rmu_co8505711.1_g000001 Rmu_sc0002193.1_g000017 Rmu_sc0006198.1_g000006
rosa_roxburghii	Rroxscaffold_2G00147320
rosa_rugosa	Rorug02G0042500
rosa_samantha	Rh2AG090100 Rh2BG090100 Rh2CG092600 Rh2DG089000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	491, 1709
Acc65I	GGTACC	1 cut(s)	1835
AccB1I	GGYRCC	2 cut(s)	802, 1835
AccB7I	CCANNNNNTGG	1 cut(s)	1315
AciI	CCGC	9 cut(s)	32, 278, 354, 417, 1015, 1062, 1208, 1445, 1683
AclWI	GGATC	5 cut(s)	838, 1244, 1375, 1386, 1982
AcoI	YGGCCR	1 cut(s)	150
AcsI	RAATTY	1 cut(s)	37
AcuI	CTGAAG	1 cut(s)	1119
AcvI	CACGTG	1 cut(s)	1415
AcyI	GRCGYC	1 cut(s)	227
AdeI	CACNNNGTG	2 cut(s)	325, 965
AfaI	GTAC	7 cut(s)	242, 773, 1307, 1679, 1780, 1796, 1837
AfiI	CCNNNNNNNGG	9 cut(s)	524, 818, 887, 888, 1029, 1030, 1061, 1315, 1689
AflII	CTTAAG	1 cut(s)	1786
AgeI	ACCGGT	1 cut(s)	135
AgsI	TTSAA	8 cut(s)	310, 461, 759, 1038, 1132, 1622, 1762, 1960
AhdI	GACNNNNNGTC	1 cut(s)	105
AjnI	CCWGG	4 cut(s)	553, 947, 961, 1739
AluBI	AGCT	7 cut(s)	381, 539, 860, 902, 1337, 1364, 1814
AluI	AGCT	7 cut(s)	381, 539, 860, 902, 1337, 1364, 1814
Alw21I	GWGCWC	2 cut(s)	1852, 1947
Alw26I	GTCTC	5 cut(s)	7, 53, 474, 995, 1205
Alw44I	GTGCAC	1 cut(s)	1943
AlwI	GGATC	5 cut(s)	838, 1244, 1375, 1386, 1982
AoxI	GGCC	5 cut(s)	150, 515, 877, 927, 1828
ApaLI	GTGCAC	1 cut(s)	1943
ApeKI	GCWGC	4 cut(s)	159, 381, 536, 1904
ApoI	RAATTY	1 cut(s)	37
ArsI	GACNNNNNNTTYG	2 cut(s)	125, 157
AsiGI	ACCGGT	1 cut(s)	135
Asp718I	GGTACC	1 cut(s)	1835
AspLEI	GCGC	3 cut(s)	386, 1399, 1913
AspS9I	GGNCC	5 cut(s)	263, 493, 673, 878, 1828
AsuC2I	CCSGG	6 cut(s)	81, 282, 323, 339, 1024, 1832
AsuHPI	GGTGA	3 cut(s)	95, 340, 1086
AvaII	GGWCC	3 cut(s)	263, 493, 673
BaeGI	GKGCMC	2 cut(s)	339, 1947
BalI	TGGCCA	1 cut(s)	152
BanI	GGYRCC	2 cut(s)	802, 1835
BanII	GRGCYC	1 cut(s)	360
BbrPI	CACGTG	1 cut(s)	1415
Bbv12I	GWGCWC	2 cut(s)	1852, 1947
BbvCI	CCTCAGC	1 cut(s)	1633
BbvI	GCAGC	4 cut(s)	146, 368, 548, 1916
BccI	CCATC	3 cut(s)	383, 1402, 1682
BciT130I	CCWGG	4 cut(s)	555, 949, 963, 1741
BcnI	CCSGG	6 cut(s)	81, 282, 323, 339, 1024, 1832
BcoDI	GTCTC	5 cut(s)	7, 53, 474, 995, 1205
BfaI	CTAG	2 cut(s)	1499, 1823
BfmI	CTRYAG	1 cut(s)	498
BfoI	RGCGCY	1 cut(s)	1914
BfrI	CTTAAG	1 cut(s)	1786
BfuAI	ACCTGC	2 cut(s)	491, 1709
BglII	AGATCT	2 cut(s)	1533, 1984
BisI	GCNGC	5 cut(s)	160, 382, 418, 537, 1905
BlpI	GCTNAGC	1 cut(s)	846
BlsI	GCNGC	5 cut(s)	161, 383, 419, 538, 1906
Bme18I	GGWCC	3 cut(s)	263, 493, 673
BmeRI	GACNNNNNGTC	1 cut(s)	105
BmgT120I	GGNCC	5 cut(s)	263, 493, 673, 878, 1828
BmsI	GCATC	6 cut(s)	1002, 1108, 1141, 1240, 1411, 1566
BpmI	CTGGAG	2 cut(s)	1762, 1794
Bpu10I	CCTNAGC	1 cut(s)	1633
Bpu1102I	GCTNAGC	1 cut(s)	846
BpuMI	CCSGG	6 cut(s)	81, 282, 323, 339, 1024, 1832
Bsa29I	ATCGAT	1 cut(s)	1239
BsaAI	YACGTR	2 cut(s)	244, 1415
BsaHI	GRCGYC	1 cut(s)	227
BsaI	GGTCTC	1 cut(s)	995
BsaJI	CCNNGG	3 cut(s)	553, 948, 1831
BsaWI	WCCGGW	2 cut(s)	135, 265
BsaXI	ACNNNNNCTCC	2 cut(s)	1926, 1956
Bsc4I	CCNNNNNNNGG	9 cut(s)	524, 818, 887, 888, 1029, 1030, 1061, 1315, 1689
Bse118I	RCCGGY	4 cut(s)	135, 223, 350, 1705
Bse1I	ACTGG	3 cut(s)	490, 495, 1094
Bse3DI	GCAATG	2 cut(s)	225, 936
BseBI	CCWGG	4 cut(s)	555, 949, 963, 1741
BseCI	ATCGAT	1 cut(s)	1239
BseDI	CCNNGG	3 cut(s)	553, 948, 1831
BseGI	GGATG	8 cut(s)	985, 1023, 1255, 1426, 1462, 1557, 1699, 1894
BseLI	CCNNNNNNNGG	9 cut(s)	524, 818, 887, 888, 1029, 1030, 1061, 1315, 1689
BseMI	GCAATG	2 cut(s)	225, 936
BseMII	CTCAG	2 cut(s)	251, 1647
BseNI	ACTGG	3 cut(s)	490, 495, 1094
BseRI	GAGGAG	2 cut(s)	101, 623
BseSI	GKGCMC	2 cut(s)	339, 1947
BseXI	GCAGC	4 cut(s)	146, 368, 548, 1916
BseYI	CCCAGC	1 cut(s)	453
BsgI	GTGCAG	1 cut(s)	1737
BshFI	GGCC	5 cut(s)	152, 517, 879, 929, 1830
BshNI	GGYRCC	2 cut(s)	802, 1835
BshTI	ACCGGT	1 cut(s)	135
BshVI	ATCGAT	1 cut(s)	1239
BsiHKAI	GWGCWC	2 cut(s)	1852, 1947
BslFI	GGGAC	2 cut(s)	686, 1079
BslI	CCNNNNNNNGG	9 cut(s)	524, 818, 887, 888, 1029, 1030, 1061, 1315, 1689
BsmAI	GTCTC	5 cut(s)	7, 53, 474, 995, 1205
BsmBI	CGTCTC	2 cut(s)	7, 1205
BsmFI	GGGAC	2 cut(s)	686, 1079
BsmI	GAATGC	4 cut(s)	1216, 1282, 1610, 1773
BsnI	GGCC	5 cut(s)	152, 517, 879, 929, 1830
Bso31I	GGTCTC	1 cut(s)	995
Bsp1286I	GDGCHC	4 cut(s)	339, 360, 1852, 1947
Bsp143I	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
Bsp1720I	GCTNAGC	1 cut(s)	846
BspACI	CCGC	9 cut(s)	32, 278, 354, 417, 1015, 1062, 1208, 1445, 1683
BspANI	GGCC	5 cut(s)	152, 517, 879, 929, 1830
BspCNI	CTCAG	2 cut(s)	250, 1646
BspDI	ATCGAT	1 cut(s)	1239
BspMAI	CTGCAG	1 cut(s)	502
BspMI	ACCTGC	2 cut(s)	491, 1709
BspPI	GGATC	5 cut(s)	838, 1244, 1375, 1386, 1982
BspT107I	GGYRCC	2 cut(s)	802, 1835
BspTI	CTTAAG	1 cut(s)	1786
BspTNI	GGTCTC	1 cut(s)	995
BsrDI	GCAATG	2 cut(s)	225, 936
BsrFI	RCCGGY	4 cut(s)	135, 223, 350, 1705
BsrI	ACTGG	3 cut(s)	490, 495, 1094
BssAI	RCCGGY	4 cut(s)	135, 223, 350, 1705
BssECI	CCNNGG	3 cut(s)	553, 948, 1831
BssMI	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
BssNI	GRCGYC	1 cut(s)	227
Bst2UI	CCWGG	4 cut(s)	555, 949, 963, 1741
Bst4CI	ACNGT	1 cut(s)	1702
Bst6I	CTCTTC	1 cut(s)	398
BstACI	GRCGYC	1 cut(s)	227
BstAFI	CTTAAG	1 cut(s)	1786
BstBAI	YACGTR	2 cut(s)	244, 1415
BstC8I	GCNNGC	5 cut(s)	164, 225, 356, 595, 1909
BstDEI	CTNAG	4 cut(s)	11, 237, 846, 1633
BstEII	GGTNACC	1 cut(s)	1092
BstF5I	GGATG	8 cut(s)	985, 1023, 1255, 1426, 1462, 1557, 1699, 1894
BstH2I	RGCGCY	1 cut(s)	1914
BstHHI	GCGC	3 cut(s)	386, 1399, 1913
BstKTI	GATC	8 cut(s)	189, 833, 1239, 1370, 1381, 1536, 1977, 1987
BstMAI	GTCTC	5 cut(s)	7, 53, 474, 995, 1205
BstMBI	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
BstMWI	GCNNNNNNNGC	5 cut(s)	208, 224, 590, 1811, 1910
BstNI	CCWGG	4 cut(s)	555, 949, 963, 1741
BstPI	GGTNACC	1 cut(s)	1092
BstSFI	CTRYAG	1 cut(s)	498
BstSLI	GKGCMC	2 cut(s)	339, 1947
BstV1I	GCAGC	4 cut(s)	146, 368, 548, 1916
BstX2I	RGATCY	3 cut(s)	1378, 1533, 1984
BstXI	CCANNNNNNTGG	3 cut(s)	453, 625, 1408
BstYI	RGATCY	3 cut(s)	1378, 1533, 1984
Bsu15I	ATCGAT	1 cut(s)	1239
BsuRI	GGCC	5 cut(s)	152, 517, 879, 929, 1830
BsuTUI	ATCGAT	1 cut(s)	1239
BtsCI	GGATG	8 cut(s)	985, 1023, 1255, 1426, 1462, 1557, 1699, 1894
BveI	ACCTGC	2 cut(s)	491, 1709
Cac8I	GCNNGC	5 cut(s)	164, 225, 356, 595, 1909
CfoI	GCGC	3 cut(s)	386, 1399, 1913
Cfr10I	RCCGGY	4 cut(s)	135, 223, 350, 1705
Cfr13I	GGNCC	5 cut(s)	263, 493, 673, 878, 1828
ClaI	ATCGAT	1 cut(s)	1239
CseI	GACGC	3 cut(s)	95, 216, 1222
CsiI	ACCWGGT	1 cut(s)	961
Csp6I	GTAC	7 cut(s)	241, 772, 1306, 1678, 1779, 1795, 1836
CspAI	ACCGGT	1 cut(s)	135
CviAII	CATG	7 cut(s)	619, 1029, 1310, 1315, 1726, 1802, 1853
CviQI	GTAC	7 cut(s)	241, 772, 1306, 1678, 1779, 1795, 1836
DdeI	CTNAG	4 cut(s)	11, 237, 846, 1633
DpnI	GATC	8 cut(s)	188, 832, 1238, 1369, 1380, 1535, 1976, 1986
DpnII	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
DraI	TTTAAA	1 cut(s)	1665
DraIII	CACNNNGTG	2 cut(s)	325, 965
DriI	GACNNNNNGTC	1 cut(s)	105
EaeI	YGGCCR	1 cut(s)	150
Eam1104I	CTCTTC	1 cut(s)	398
Eam1105I	GACNNNNNGTC	1 cut(s)	105
EarI	CTCTTC	1 cut(s)	398
EciI	GGCGGA	1 cut(s)	47
Eco147I	AGGCCT	1 cut(s)	517
Eco24I	GRGCYC	1 cut(s)	360
Eco31I	GGTCTC	1 cut(s)	995
Eco47I	GGWCC	3 cut(s)	263, 493, 673
Eco57I	CTGAAG	1 cut(s)	1119
Eco72I	CACGTG	1 cut(s)	1415
Eco91I	GGTNACC	1 cut(s)	1092
EcoO65I	GGTNACC	1 cut(s)	1092
EcoRII	CCWGG	4 cut(s)	553, 947, 961, 1739
EcoT38I	GRGCYC	1 cut(s)	360
Esp3I	CGTCTC	2 cut(s)	7, 1205
FaeI	CATG	7 cut(s)	622, 1032, 1313, 1318, 1729, 1805, 1856
FaqI	GGGAC	2 cut(s)	686, 1079
FatI	CATG	7 cut(s)	618, 1028, 1309, 1314, 1725, 1801, 1852
FauI	CCCGC	3 cut(s)	285, 347, 1055
FauNDI	CATATG	6 cut(s)	179, 465, 634, 1008, 1162, 1576
Fnu4HI	GCNGC	5 cut(s)	160, 382, 418, 537, 1905
FokI	GGATG	8 cut(s)	992, 1030, 1262, 1433, 1469, 1544, 1706, 1901
FriOI	GRGCYC	1 cut(s)	360
Fsp4HI	GCNGC	5 cut(s)	160, 382, 418, 537, 1905
FspBI	CTAG	2 cut(s)	1499, 1823
GlaI	GCGC	3 cut(s)	385, 1398, 1912
GluI	GCNGC	5 cut(s)	160, 382, 418, 537, 1905
GsaI	CCCAGC	1 cut(s)	457
GsuI	CTGGAG	2 cut(s)	1762, 1794
HaeII	RGCGCY	1 cut(s)	1914
HaeIII	GGCC	5 cut(s)	152, 517, 879, 929, 1830
HgaI	GACGC	3 cut(s)	95, 216, 1222
HhaI	GCGC	3 cut(s)	386, 1399, 1913
Hin1I	GRCGYC	1 cut(s)	227
Hin1II	CATG	7 cut(s)	622, 1032, 1313, 1318, 1729, 1805, 1856
Hin6I	GCGC	3 cut(s)	384, 1397, 1911
HinP1I	GCGC	3 cut(s)	384, 1397, 1911
HincII	GTYRAC	1 cut(s)	1590
HindII	GTYRAC	1 cut(s)	1590
HinfI	GANTC	2 cut(s)	174, 1034
HpaI	GTTAAC	1 cut(s)	1590
HphI	GGTGA	3 cut(s)	95, 340, 1086
Hpy166II	GTNNAC	3 cut(s)	1590, 1678, 1945
Hpy188III	TCNNGA	4 cut(s)	473, 1085, 1523, 1823
Hpy8I	GTNNAC	3 cut(s)	1590, 1678, 1945
Hpy99I	CGWCG	4 cut(s)	103, 111, 114, 232
HpyAV	CCTTC	7 cut(s)	22, 61, 111, 292, 304, 365, 1776
HpyCH4III	ACNGT	1 cut(s)	1702
HpyCH4IV	ACGT	3 cut(s)	243, 1203, 1414
HpyCH4V	TGCA	6 cut(s)	500, 781, 959, 1154, 1718, 1945
HpyF10VI	GCNNNNNNNGC	5 cut(s)	208, 224, 590, 1811, 1910
HpyF3I	CTNAG	4 cut(s)	11, 237, 846, 1633
HpySE526I	ACGT	3 cut(s)	243, 1203, 1414
Hsp92I	GRCGYC	1 cut(s)	227
Hsp92II	CATG	7 cut(s)	622, 1032, 1313, 1318, 1729, 1805, 1856
HspAI	GCGC	3 cut(s)	384, 1397, 1911
KpnI	GGTACC	1 cut(s)	1839
KroI	GCCGGC	1 cut(s)	223
KroNI	GCCGGC	1 cut(s)	225
KspAI	GTTAAC	1 cut(s)	1590
Kzo9I	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
LmnI	GCTCC	2 cut(s)	43, 1525
Lsp1109I	GCAGC	4 cut(s)	146, 368, 548, 1916
LweI	GCATC	6 cut(s)	1002, 1108, 1141, 1240, 1411, 1566
MabI	ACCWGGT	1 cut(s)	961
MaeI	CTAG	2 cut(s)	1499, 1823
MaeII	ACGT	3 cut(s)	243, 1203, 1414
MaeIII	GTNAC	4 cut(s)	244, 346, 557, 1092
MalI	GATC	8 cut(s)	188, 832, 1238, 1369, 1380, 1535, 1976, 1986
MboI	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
MboII	GAAGA	5 cut(s)	415, 1112, 1135, 1144, 1811
MflI	RGATCY	3 cut(s)	1378, 1533, 1984
MhlI	GDGCHC	4 cut(s)	339, 360, 1852, 1947
MlsI	TGGCCA	1 cut(s)	152
MluNI	TGGCCA	1 cut(s)	152
MlyI	GAGTC	1 cut(s)	183
MmeI	TCCRAC	2 cut(s)	92, 297
Mox20I	TGGCCA	1 cut(s)	152
MroNI	GCCGGC	1 cut(s)	223
MscI	TGGCCA	1 cut(s)	152
MseI	TTAA	8 cut(s)	578, 855, 1257, 1589, 1664, 1787, 1880, 1895
MslI	CAYNNNNRTG	3 cut(s)	623, 1116, 1290
Msp20I	TGGCCA	1 cut(s)	152
MspA1I	CMGCKG	3 cut(s)	539, 1364, 1685
MspCI	CTTAAG	1 cut(s)	1786
Mva1269I	GAATGC	4 cut(s)	1216, 1282, 1610, 1773
MvaI	CCWGG	4 cut(s)	555, 949, 963, 1741
MwoI	GCNNNNNNNGC	5 cut(s)	208, 224, 590, 1811, 1910
NaeI	GCCGGC	1 cut(s)	225
NciI	CCSGG	6 cut(s)	81, 282, 323, 339, 1024, 1832
NdeI	CATATG	6 cut(s)	179, 465, 634, 1008, 1162, 1576
NdeII	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
NgoMIV	GCCGGC	1 cut(s)	223
NlaIII	CATG	7 cut(s)	622, 1032, 1313, 1318, 1729, 1805, 1856
NmuCI	GTSAC	3 cut(s)	244, 346, 1092
PceI	AGGCCT	1 cut(s)	517
PctI	GAATGC	4 cut(s)	1216, 1282, 1610, 1773
PdiI	GCCGGC	1 cut(s)	225
PfeI	GAWTC	1 cut(s)	1034
PflMI	CCANNNNNTGG	1 cut(s)	1315
PinAI	ACCGGT	1 cut(s)	135
PkrI	GCNGC	5 cut(s)	161, 383, 419, 538, 1906
PleI	GAGTC	1 cut(s)	182
PmaCI	CACGTG	1 cut(s)	1415
PmlI	CACGTG	1 cut(s)	1415
PpsI	GAGTC	1 cut(s)	182
Ppu21I	YACGTR	2 cut(s)	244, 1415
Psp6I	CCWGG	4 cut(s)	553, 947, 961, 1739
PspCI	CACGTG	1 cut(s)	1415
PspEI	GGTNACC	1 cut(s)	1092
PspFI	CCCAGC	1 cut(s)	453
PspGI	CCWGG	4 cut(s)	553, 947, 961, 1739
PspPI	GGNCC	5 cut(s)	263, 493, 673, 878, 1828
PstI	CTGCAG	1 cut(s)	502
PsuI	RGATCY	3 cut(s)	1378, 1533, 1984
PvuII	CAGCTG	2 cut(s)	539, 1364
RsaI	GTAC	7 cut(s)	242, 773, 1307, 1679, 1780, 1796, 1837
RsaNI	GTAC	7 cut(s)	241, 772, 1306, 1678, 1779, 1795, 1836
RseI	CAYNNNNRTG	3 cut(s)	623, 1116, 1290
SaqAI	TTAA	8 cut(s)	578, 855, 1257, 1589, 1664, 1787, 1880, 1895
SatI	GCNGC	5 cut(s)	160, 382, 418, 537, 1905
Sau3AI	GATC	8 cut(s)	186, 830, 1236, 1367, 1378, 1533, 1974, 1984
Sau96I	GGNCC	5 cut(s)	263, 493, 673, 878, 1828
SbfI	CCTGCAGG	1 cut(s)	502
SchI	GAGTC	1 cut(s)	183
SdaI	CCTGCAGG	1 cut(s)	502
SduI	GDGCHC	4 cut(s)	339, 360, 1852, 1947
SexAI	ACCWGGT	1 cut(s)	961
SfaNI	GCATC	6 cut(s)	1002, 1108, 1141, 1240, 1411, 1566
SfcI	CTRYAG	1 cut(s)	498
SgrAI	CRCCGGYG	1 cut(s)	350
SinI	GGWCC	3 cut(s)	263, 493, 673
SmiMI	CAYNNNNRTG	3 cut(s)	623, 1116, 1290
SmlI	CTYRAG	1 cut(s)	1786
SmoI	CTYRAG	1 cut(s)	1786
Sse8387I	CCTGCAGG	1 cut(s)	502
SseBI	AGGCCT	1 cut(s)	517
SsiI	CCGC	9 cut(s)	32, 278, 354, 417, 1015, 1062, 1208, 1445, 1683
SspI	AATATT	1 cut(s)	1484
SspMI	CTAG	2 cut(s)	1499, 1823
StuI	AGGCCT	1 cut(s)	517
TaaI	ACNGT	1 cut(s)	1702
TaiI	ACGT	3 cut(s)	246, 1206, 1417
TaqI	TCGA	3 cut(s)	185, 360, 1239
TatI	WGTACW	2 cut(s)	771, 1677
TauI	GCSGC	1 cut(s)	420
TfiI	GAWTC	1 cut(s)	1034
Tru1I	TTAA	8 cut(s)	578, 855, 1257, 1589, 1664, 1787, 1880, 1895
Tru9I	TTAA	8 cut(s)	578, 855, 1257, 1589, 1664, 1787, 1880, 1895
TseFI	GTSAC	3 cut(s)	244, 346, 1092
TseI	GCWGC	4 cut(s)	159, 381, 536, 1904
Tsp45I	GTSAC	3 cut(s)	244, 346, 1092
TspDTI	ATGAA	9 cut(s)	495, 621, 803, 881, 1376, 1485, 1710, 1714, 1938
TspGWI	ACGGA	2 cut(s)	31, 263
Van91I	CCANNNNNTGG	1 cut(s)	1315
Vha464I	CTTAAG	1 cut(s)	1786
VneI	GTGCAC	1 cut(s)	1943
VpaK11BI	GGWCC	3 cut(s)	263, 493, 673
XapI	RAATTY	1 cut(s)	37
XbaI	TCTAGA	1 cut(s)	1822
XspI	CTAG	2 cut(s)	1499, 1823

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.