Rh2BG090100

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009041
Physical Location & Seq
Reverse (-)
7011457 .. 7016662
5206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG090100.1

Sequence Viewer

Length: 1527 bp
ATGTCTCTGGACAATGAAACTGGACTGGACCCTGCAGGTATAAGGGTTAGGCCTGTATCTGGTCTTGTGGCAGCTGATTACTTTGCCCCTGGTTACTTTGTGTGGGCAGTTTTAATTGCTAATTTGGCTCGCATTGGATATGAGGAGAAAACCATGTATATGGCTTCATATGATTGGAGATTATCGTTTCAGAATACAGAGGTTAGGGACCAAACTTTGAGCAGAATAAAAAGTAATATAGAACTTATGGTAGCTACAAATGGTGGCAACAAGGTGGTTGTTCTCCCACATTCAATGGGTGTTCTGTACTTTCTGCATTTTATGAAATGGGTTGAGGCACCAGCACCAATGGGAGGTGGAGGTGGATCAGATTGGTGTGCTAAGCATATTAAAGCTGTAATGAACATCGGTGGCCCCTTTTTAGGGGTTCCAAAAGCTGTTGCTGGACTTTTCTCTATTGAGGCCAAAGACATTGCTGTTGCCAGGGCTTTTGCACCAGGTGTTTTGGATAAGGATGTTTTTGGTCTCCAAACCTTACAACATATGATGCGGATGACCCGGACATGGGATTCAACTATGTCTATGATACCAAAAGGTGGGGACACAATATGGGGTGGTCTTGACTGGTCACCTGAAGAATACTGCCATTGTGATGCGAAGAAGTTGAAGAATAATGATACCAAAGATGCAGAGCCATATGGAGTAAATTATGGGAGGATTATATCATTTGGGAAAGACGTAGCGGAGACGCATTCCTCCAAGATTGAGAGGATCGATTTTAGGGATGCTGTTAAGGGTAATTTACTTGCGAATGCTACCCATTGTGATGTATGGACAGAGTACCATGACATGGGTGTTGGGGGTGTCAAAGCTGTTTCAGATTACAAAATCTACACAGCTGGATCAATTTTGGATCTGCTTCATTTTGTTGCGCCCAAATTGATGGCACGTGGGGATGCTCATTTTTCGTATGGAATTGCGGACAATTTGGATGACCCCAAGTATGAACACTACAAATATTGGTCAAACCCCCTAGAAACAAGATTACCAAATGCTCCTGATATGGAGATCTATTCTATGTATGGAGTTGGCATCCCTACCGAAAGAGCATATGTGTATAAGTTAACTCCTACTGCTGAATGCTATATTCCCTTTCAAATAGATACCTCAGCAGAGGGTGGAAGTGACAACCAGTGTTTAAAAGGTGGAGTGTACTCCGCTGATGGGGATGAAACTGTGCCGGTGTTGAGTGCAGGTTTCATGTGTGCTAAAGCCTGGAGAGGAAAGACCCGTTTCAACCCTTCTGGCATTCATACTTACTTAAGGGAGTACGACCATGCCCCTCCAGCTAATCTTCTAGAGGGCCGGGGTACCCAAAGTGGTGCTCATGTTGATATAATGGGAAACTTTGCTTTAATTGAGGATGTTTTAAGAGTAGCAGCAGGCGCTACGGGAGATGAATTGGGTGGAGATAAAGTGCACTCTGATATTTTCAAGTGGTCTGAAAGGATCAATATAGATCTCTGA

Protein Analysis

508

Amino Acids

55.92

Weight (kDa)

5.54

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 3 - 468 4.8e-58 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 26, 1244
Acc65I GGTACC 1 cut(s) 1370
AccB1I GGYRCC 2 cut(s) 337, 1370
AccB7I CCANNNNNTGG 2 cut(s) 596, 850
AciI CCGC 4 cut(s) 550, 743, 980, 1218
AclWI GGATC 5 cut(s) 373, 779, 910, 921, 1517
AcuI CTGAAG 1 cut(s) 654
AcvI CACGTG 1 cut(s) 950
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 5 cut(s) 308, 842, 1214, 1331, 1372
AfiI CCNNNNNNNGG 9 cut(s) 59, 353, 422, 423, 564, 565, 596, 850, 1224
AflII CTTAAG 1 cut(s) 1321
AgsI TTSAA 6 cut(s) 294, 573, 667, 1157, 1297, 1495
AjnI CCWGG 4 cut(s) 88, 482, 496, 1274
AluBI AGCT 7 cut(s) 74, 254, 395, 437, 872, 899, 1349
AluI AGCT 7 cut(s) 74, 254, 395, 437, 872, 899, 1349
Alw21I GWGCWC 2 cut(s) 1387, 1482
Alw26I GTCTC 3 cut(s) 9, 530, 740
Alw44I GTGCAC 1 cut(s) 1478
AlwI GGATC 5 cut(s) 373, 779, 910, 921, 1517
AoxI GGCC 4 cut(s) 50, 412, 462, 1363
ApaLI GTGCAC 1 cut(s) 1478
ApeKI GCWGC 2 cut(s) 71, 1439
Asp718I GGTACC 1 cut(s) 1370
AspLEI GCGC 2 cut(s) 934, 1448
AspS9I GGNCC 4 cut(s) 28, 208, 413, 1363
AsuC2I CCSGG 2 cut(s) 559, 1367
AsuHPI GGTGA 1 cut(s) 621
AvaII GGWCC 2 cut(s) 28, 208
BaeGI GKGCMC 1 cut(s) 1482
BanI GGYRCC 2 cut(s) 337, 1370
BbrPI CACGTG 1 cut(s) 950
Bbv12I GWGCWC 2 cut(s) 1387, 1482
BbvCI CCTCAGC 1 cut(s) 1168
BbvI GCAGC 2 cut(s) 83, 1451
BccI CCATC 2 cut(s) 937, 1217
BciT130I CCWGG 4 cut(s) 90, 484, 498, 1276
BcnI CCSGG 2 cut(s) 559, 1367
BcoDI GTCTC 3 cut(s) 9, 530, 740
BfaI CTAG 2 cut(s) 1034, 1358
BfmI CTRYAG 1 cut(s) 33
BfoI RGCGCY 1 cut(s) 1449
BfrI CTTAAG 1 cut(s) 1321
BfuAI ACCTGC 2 cut(s) 26, 1244
BglII AGATCT 2 cut(s) 1068, 1519
BisI GCNGC 2 cut(s) 72, 1440
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 2 cut(s) 73, 1441
Bme1390I CCNGG 6 cut(s) 90, 484, 498, 559, 1276, 1367
Bme18I GGWCC 2 cut(s) 28, 208
BmgT120I GGNCC 4 cut(s) 28, 208, 413, 1363
BmiI GGNNCC 6 cut(s) 30, 209, 339, 415, 429, 1372
BmrFI CCNGG 6 cut(s) 90, 484, 498, 559, 1276, 1367
BmsI GCATC 6 cut(s) 537, 643, 676, 775, 946, 1101
BpmI CTGGAG 2 cut(s) 1297, 1329
Bpu10I CCTNAGC 1 cut(s) 1168
Bpu1102I GCTNAGC 1 cut(s) 381
BpuMI CCSGG 2 cut(s) 559, 1367
Bsa29I ATCGAT 1 cut(s) 774
BsaAI YACGTR 1 cut(s) 950
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 3 cut(s) 88, 483, 1366
BsaXI ACNNNNNCTCC 2 cut(s) 1461, 1491
Bsc4I CCNNNNNNNGG 9 cut(s) 59, 353, 422, 423, 564, 565, 596, 850, 1224
Bse118I RCCGGY 1 cut(s) 1240
Bse1I ACTGG 4 cut(s) 25, 30, 629, 1192
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 4 cut(s) 90, 484, 498, 1276
BseCI ATCGAT 1 cut(s) 774
BseDI CCNNGG 3 cut(s) 88, 483, 1366
BseGI GGATG 8 cut(s) 520, 558, 790, 961, 997, 1092, 1234, 1429
BseLI CCNNNNNNNGG 9 cut(s) 59, 353, 422, 423, 564, 565, 596, 850, 1224
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 1182
BseNI ACTGG 4 cut(s) 25, 30, 629, 1192
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 1482
BseXI GCAGC 2 cut(s) 83, 1451
BsgI GTGCAG 1 cut(s) 1272
BshFI GGCC 4 cut(s) 52, 414, 464, 1365
BshNI GGYRCC 2 cut(s) 337, 1370
BshVI ATCGAT 1 cut(s) 774
BsiHKAI GWGCWC 2 cut(s) 1387, 1482
BsiSI CCGG 3 cut(s) 559, 1241, 1366
BslFI GGGAC 2 cut(s) 221, 614
BslI CCNNNNNNNGG 9 cut(s) 59, 353, 422, 423, 564, 565, 596, 850, 1224
BsmAI GTCTC 3 cut(s) 9, 530, 740
BsmBI CGTCTC 1 cut(s) 740
BsmFI GGGAC 2 cut(s) 221, 614
BsmI GAATGC 4 cut(s) 751, 817, 1145, 1308
BsnI GGCC 4 cut(s) 52, 414, 464, 1365
Bso31I GGTCTC 1 cut(s) 530
Bsp1286I GDGCHC 2 cut(s) 1387, 1482
Bsp143I GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 4 cut(s) 550, 743, 980, 1218
BspANI GGCC 4 cut(s) 52, 414, 464, 1365
BspCNI CTCAG 1 cut(s) 1181
BspDI ATCGAT 1 cut(s) 774
BspLI GGNNCC 6 cut(s) 30, 209, 339, 415, 429, 1372
BspMAI CTGCAG 1 cut(s) 37
BspMI ACCTGC 2 cut(s) 26, 1244
BspPI GGATC 5 cut(s) 373, 779, 910, 921, 1517
BspT107I GGYRCC 2 cut(s) 337, 1370
BspTI CTTAAG 1 cut(s) 1321
BspTNI GGTCTC 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 1 cut(s) 1240
BsrI ACTGG 4 cut(s) 25, 30, 629, 1192
BssAI RCCGGY 1 cut(s) 1240
BssECI CCNNGG 3 cut(s) 88, 483, 1366
BssMI GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
Bst2UI CCWGG 4 cut(s) 90, 484, 498, 1276
Bst4CI ACNGT 1 cut(s) 1237
BstAFI CTTAAG 1 cut(s) 1321
BstBAI YACGTR 1 cut(s) 950
BstC8I GCNNGC 2 cut(s) 130, 1444
BstDEI CTNAG 2 cut(s) 381, 1168
BstEII GGTNACC 1 cut(s) 627
BstF5I GGATG 8 cut(s) 520, 558, 790, 961, 997, 1092, 1234, 1429
BstH2I RGCGCY 1 cut(s) 1449
BstHHI GCGC 2 cut(s) 934, 1448
BstKTI GATC 7 cut(s) 368, 774, 905, 916, 1071, 1512, 1522
BstMAI GTCTC 3 cut(s) 9, 530, 740
BstMBI GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
BstMWI GCNNNNNNNGC 3 cut(s) 125, 1346, 1445
BstNI CCWGG 4 cut(s) 90, 484, 498, 1276
BstPI GGTNACC 1 cut(s) 627
BstSCI CCNGG 6 cut(s) 88, 482, 496, 557, 1274, 1365
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 1482
BstV1I GCAGC 2 cut(s) 83, 1451
BstX2I RGATCY 3 cut(s) 913, 1068, 1519
BstXI CCANNNNNNTGG 2 cut(s) 160, 943
BstYI RGATCY 3 cut(s) 913, 1068, 1519
Bsu15I ATCGAT 1 cut(s) 774
BsuRI GGCC 4 cut(s) 52, 414, 464, 1365
BsuTUI ATCGAT 1 cut(s) 774
BtsCI GGATG 8 cut(s) 520, 558, 790, 961, 997, 1092, 1234, 1429
BtsIMutI CAGTG 1 cut(s) 1199
BveI ACCTGC 2 cut(s) 26, 1244
Cac8I GCNNGC 2 cut(s) 130, 1444
CfoI GCGC 2 cut(s) 934, 1448
Cfr10I RCCGGY 1 cut(s) 1240
Cfr13I GGNCC 4 cut(s) 28, 208, 413, 1363
ClaI ATCGAT 1 cut(s) 774
CseI GACGC 1 cut(s) 757
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 5 cut(s) 307, 841, 1213, 1330, 1371
CviAII CATG 7 cut(s) 154, 564, 845, 850, 1261, 1337, 1388
CviQI GTAC 5 cut(s) 307, 841, 1213, 1330, 1371
DdeI CTNAG 2 cut(s) 381, 1168
DpnI GATC 7 cut(s) 367, 773, 904, 915, 1070, 1511, 1521
DpnII GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
DraI TTTAAA 1 cut(s) 1200
DraIII CACNNNGTG 1 cut(s) 500
Eco147I AGGCCT 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 2 cut(s) 28, 208
Eco57I CTGAAG 1 cut(s) 654
Eco72I CACGTG 1 cut(s) 950
Eco91I GGTNACC 1 cut(s) 627
EcoO65I GGTNACC 1 cut(s) 627
EcoRII CCWGG 4 cut(s) 88, 482, 496, 1274
Esp3I CGTCTC 1 cut(s) 740
FaeI CATG 7 cut(s) 157, 567, 848, 853, 1264, 1340, 1391
FaqI GGGAC 2 cut(s) 221, 614
FatI CATG 7 cut(s) 153, 563, 844, 849, 1260, 1336, 1387
FauNDI CATATG 4 cut(s) 169, 543, 697, 1111
Fnu4HI GCNGC 2 cut(s) 72, 1440
FokI GGATG 8 cut(s) 527, 565, 797, 968, 1004, 1079, 1241, 1436
Fsp4HI GCNGC 2 cut(s) 72, 1440
FspBI CTAG 2 cut(s) 1034, 1358
GlaI GCGC 2 cut(s) 933, 1447
GluI GCNGC 2 cut(s) 72, 1440
GsuI CTGGAG 2 cut(s) 1297, 1329
HaeII RGCGCY 1 cut(s) 1449
HaeIII GGCC 4 cut(s) 52, 414, 464, 1365
HapII CCGG 3 cut(s) 559, 1241, 1366
HgaI GACGC 1 cut(s) 757
HhaI GCGC 2 cut(s) 934, 1448
Hin1II CATG 7 cut(s) 157, 567, 848, 853, 1264, 1340, 1391
Hin6I GCGC 2 cut(s) 932, 1446
HinP1I GCGC 2 cut(s) 932, 1446
HincII GTYRAC 1 cut(s) 1125
HindII GTYRAC 1 cut(s) 1125
HinfI GANTC 1 cut(s) 569
HpaI GTTAAC 1 cut(s) 1125
HpaII CCGG 3 cut(s) 559, 1241, 1366
HphI GGTGA 1 cut(s) 621
Hpy166II GTNNAC 3 cut(s) 1125, 1213, 1480
Hpy188I TCNGA 6 cut(s) 192, 370, 880, 1486, 1504, 1526
Hpy188III TCNNGA 4 cut(s) 8, 620, 1058, 1358
Hpy8I GTNNAC 3 cut(s) 1125, 1213, 1480
HpyAV CCTTC 1 cut(s) 1311
HpyCH4III ACNGT 1 cut(s) 1237
HpyCH4IV ACGT 2 cut(s) 738, 949
HpyCH4V TGCA 6 cut(s) 35, 316, 494, 689, 1253, 1480
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 1346, 1445
HpyF3I CTNAG 2 cut(s) 381, 1168
HpySE526I ACGT 2 cut(s) 738, 949
Hsp92II CATG 7 cut(s) 157, 567, 848, 853, 1264, 1340, 1391
HspAI GCGC 2 cut(s) 932, 1446
KpnI GGTACC 1 cut(s) 1374
KspAI GTTAAC 1 cut(s) 1125
Kzo9I GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
LmnI GCTCC 1 cut(s) 1060
Lsp1109I GCAGC 2 cut(s) 83, 1451
LweI GCATC 6 cut(s) 537, 643, 676, 775, 946, 1101
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 2 cut(s) 1034, 1358
MaeII ACGT 2 cut(s) 738, 949
MaeIII GTNAC 3 cut(s) 92, 627, 1184
MalI GATC 7 cut(s) 367, 773, 904, 915, 1070, 1511, 1521
MboI GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
MboII GAAGA 4 cut(s) 647, 670, 679, 1346
MflI RGATCY 3 cut(s) 913, 1068, 1519
MhlI GDGCHC 2 cut(s) 1387, 1482
MseI TTAA 8 cut(s) 113, 390, 792, 1124, 1199, 1322, 1415, 1430
MslI CAYNNNNRTG 3 cut(s) 158, 651, 825
MspA1I CMGCKG 3 cut(s) 74, 899, 1220
MspCI CTTAAG 1 cut(s) 1321
MspI CCGG 3 cut(s) 559, 1241, 1366
MspR9I CCNGG 6 cut(s) 90, 484, 498, 559, 1276, 1367
Mva1269I GAATGC 4 cut(s) 751, 817, 1145, 1308
MvaI CCWGG 4 cut(s) 90, 484, 498, 1276
MwoI GCNNNNNNNGC 3 cut(s) 125, 1346, 1445
NciI CCSGG 2 cut(s) 559, 1367
NdeI CATATG 4 cut(s) 169, 543, 697, 1111
NdeII GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
NlaIII CATG 7 cut(s) 157, 567, 848, 853, 1264, 1340, 1391
NlaIV GGNNCC 6 cut(s) 30, 209, 339, 415, 429, 1372
NmuCI GTSAC 2 cut(s) 627, 1184
PceI AGGCCT 1 cut(s) 52
PctI GAATGC 4 cut(s) 751, 817, 1145, 1308
PfeI GAWTC 1 cut(s) 569
PflMI CCANNNNNTGG 2 cut(s) 596, 850
PkrI GCNGC 2 cut(s) 73, 1441
PmaCI CACGTG 1 cut(s) 950
PmlI CACGTG 1 cut(s) 950
Ppu21I YACGTR 1 cut(s) 950
Psp6I CCWGG 4 cut(s) 88, 482, 496, 1274
PspCI CACGTG 1 cut(s) 950
PspEI GGTNACC 1 cut(s) 627
PspGI CCWGG 4 cut(s) 88, 482, 496, 1274
PspN4I GGNNCC 6 cut(s) 30, 209, 339, 415, 429, 1372
PspPI GGNCC 4 cut(s) 28, 208, 413, 1363
PstI CTGCAG 1 cut(s) 37
PsuI RGATCY 3 cut(s) 913, 1068, 1519
PvuII CAGCTG 2 cut(s) 74, 899
RsaI GTAC 5 cut(s) 308, 842, 1214, 1331, 1372
RsaNI GTAC 5 cut(s) 307, 841, 1213, 1330, 1371
RseI CAYNNNNRTG 3 cut(s) 158, 651, 825
SaqAI TTAA 8 cut(s) 113, 390, 792, 1124, 1199, 1322, 1415, 1430
SatI GCNGC 2 cut(s) 72, 1440
Sau3AI GATC 7 cut(s) 365, 771, 902, 913, 1068, 1509, 1519
Sau96I GGNCC 4 cut(s) 28, 208, 413, 1363
SbfI CCTGCAGG 1 cut(s) 37
ScrFI CCNGG 6 cut(s) 90, 484, 498, 559, 1276, 1367
SdaI CCTGCAGG 1 cut(s) 37
SduI GDGCHC 2 cut(s) 1387, 1482
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 6 cut(s) 537, 643, 676, 775, 946, 1101
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 2 cut(s) 28, 208
SmiMI CAYNNNNRTG 3 cut(s) 158, 651, 825
SmlI CTYRAG 1 cut(s) 1321
SmoI CTYRAG 1 cut(s) 1321
Sse8387I CCTGCAGG 1 cut(s) 37
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 4 cut(s) 550, 743, 980, 1218
SspI AATATT 1 cut(s) 1019
SspMI CTAG 2 cut(s) 1034, 1358
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 6 cut(s) 88, 482, 496, 557, 1274, 1365
TaaI ACNGT 1 cut(s) 1237
TaiI ACGT 2 cut(s) 741, 952
TaqI TCGA 1 cut(s) 774
TatI WGTACW 2 cut(s) 306, 1212
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 8 cut(s) 113, 390, 792, 1124, 1199, 1322, 1415, 1430
Tru9I TTAA 8 cut(s) 113, 390, 792, 1124, 1199, 1322, 1415, 1430
TscAI CASTG 1 cut(s) 1199
TseFI GTSAC 2 cut(s) 627, 1184
TseI GCWGC 2 cut(s) 71, 1439
Tsp45I GTSAC 2 cut(s) 627, 1184
TspRI CASTG 1 cut(s) 1199
Van91I CCANNNNNTGG 2 cut(s) 596, 850
Vha464I CTTAAG 1 cut(s) 1321
VneI GTGCAC 1 cut(s) 1478
VpaK11BI GGWCC 2 cut(s) 28, 208
XbaI TCTAGA 1 cut(s) 1357
XspI CTAG 2 cut(s) 1034, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.