RLG00000016689

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015246
Physical Location & Seq
Forward (+)
9003802 .. 9005133
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016689

Sequence Viewer

Length: 684 bp
ATGACGACTAGAATAGCTCCTGCAGTCGGAGCCGACTTGCTCGGTCAACACTCCGTTGAGAGAAACCAAGACGCTACTCTTTACGTCCGCAAAAATCTCGACCCTGAGGGCCAGCTTGAACAATCTCCACAATCAAAAGCTAGAATTATAGTTACAATCAGCTATGATTCCTTTGAGGCATCTGATGCGGCTATCGAGGCAATGCATGGCCAGTATCTGTGCTATCGTCAAATAACAGTGTTGTATGCATATAAGAAAGACTCTAAAGGGGAGCGTCATGGTACTCCGGCAGAGAGAGTTTTGGCTGCTAGCAATAATCCTACCTCTAAGACCAAGAGCCGGCCTGTTTTGCCATCGCATCCGCTTGCATATGGTGGCGTGGCTTTCCCGCCAATGCAGATGGCTGAACAACCAGCTTGGCAAGGTCAGCCTCAACAACAGGGTCAACCAATGATGCCTCCACCGCCAATGCAGCAGTTCATGAAACCTGCTCCCCCGAACATGCCGCCACCCCCTCTAGCTCCTCCACGGTCTCTTGCACCTCCTATGGCTATGGGAAACCAACAACTGATGTGGCGGCCAGCGCCTCCCCCTCAAATGAGGCCTCCGAACATGCAACATGCATCAATGCCACCACCTCCCCCACTTAACAACCCAACCCTACCGCCACCTCCAATGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

228

Amino Acids

24.88

Weight (kDa)

9.44

Isoelectric Point (pI)

71.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18510
fragaria_vesca FvH4_7g10600
malus_domestica MD07G1111200.v1.1
prunus_persica Prupe.2G134600_v2.0.a1
pyrus_communis pycom07g09600
rosa_chinensis RchiOBHm_Chr1g0348441
rosa_laevigata RLG00000016689 RLG00000028645 RLG00000028653 RLG00000028658
rosa_multiflora Rmu_sc0000091.1_g000014
rosa_roxburghii Rroxscaffold_4G00306980
rosa_rugosa Rorug01G0194600
rosa_samantha Rh1BG179000 Rh1CG197000
rosa_wichuraiana Rw1G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 496
AciI CCGC 8 cut(s) 88, 188, 362, 389, 464, 506, 577, 665
AcoI YGGCCR 2 cut(s) 208, 578
AfaI GTAC 1 cut(s) 283
AfiI CCNNNNNNNGG 2 cut(s) 26, 339
AgsI TTSAA 1 cut(s) 119
AluBI AGCT 6 cut(s) 17, 115, 140, 162, 416, 521
AluI AGCT 6 cut(s) 17, 115, 140, 162, 416, 521
Alw26I GTCTC 1 cut(s) 537
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 5 cut(s) 109, 208, 341, 578, 602
ApeKI GCWGC 2 cut(s) 305, 472
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 109
AsuNHI GCTAGC 1 cut(s) 308
AxyI CCTNAGG 1 cut(s) 105
BalI TGGCCA 1 cut(s) 210
BbvI GCAGC 2 cut(s) 292, 484
BccI CCATC 2 cut(s) 361, 394
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 4 cut(s) 9, 141, 309, 518
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 587
BfuAI ACCTGC 1 cut(s) 496
BisI GCNGC 5 cut(s) 189, 306, 473, 506, 578
BlsI GCNGC 5 cut(s) 190, 307, 474, 507, 579
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 31
BmsI GCATC 5 cut(s) 175, 188, 367, 444, 632
BmtI GCTAGC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 1 cut(s) 527
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 339
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 1 cut(s) 211
Bse21I CCTNAGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 26, 339
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 96
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 1 cut(s) 513
BseXI GCAGC 2 cut(s) 292, 484
BshFI GGCC 5 cut(s) 111, 210, 343, 580, 604
BsiSI CCGG 2 cut(s) 287, 340
BslI CCNNNNNNNGG 2 cut(s) 26, 339
BsmAI GTCTC 1 cut(s) 537
BsnI GGCC 5 cut(s) 111, 210, 343, 580, 604
Bso31I GGTCTC 1 cut(s) 537
BspACI CCGC 8 cut(s) 88, 188, 362, 389, 464, 506, 577, 665
BspANI GGCC 5 cut(s) 111, 210, 343, 580, 604
BspCNI CTCAG 1 cut(s) 97
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 31
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 496
BspOI GCTAGC 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 1 cut(s) 211
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 527
Bst4CI ACNGT 2 cut(s) 238, 531
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 5 cut(s) 113, 310, 341, 366, 582
BstDEI CTNAG 2 cut(s) 105, 327
BstDSI CCRYGG 1 cut(s) 527
BstF5I GGATG 1 cut(s) 358
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 1 cut(s) 586
BstMAI GTCTC 1 cut(s) 537
BstMWI GCNNNNNNNGC 8 cut(s) 29, 185, 197, 349, 427, 463, 472, 583
BstNSI RCATGY 3 cut(s) 505, 616, 623
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 292, 484
Bsu36I CCTNAGG 1 cut(s) 105
BsuRI GGCC 5 cut(s) 111, 210, 343, 580, 604
BtgI CCRYGG 1 cut(s) 527
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 243
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 5 cut(s) 113, 310, 341, 366, 582
CaiI CAGNNNCTG 1 cut(s) 217
CciI TCATGA 1 cut(s) 480
CfoI GCGC 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 2 cut(s) 80, 263
Csp6I GTAC 1 cut(s) 282
CspCI CAANNNNNGTGG 2 cut(s) 554, 589
CviAII CATG 6 cut(s) 206, 278, 481, 502, 613, 620
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 2 cut(s) 105, 327
EaeI YGGCCR 2 cut(s) 208, 578
Eco147I AGGCCT 1 cut(s) 604
Eco31I GGTCTC 1 cut(s) 537
Eco81I CCTNAGG 1 cut(s) 105
EcoT22I ATGCAT 3 cut(s) 207, 250, 625
FaeI CATG 6 cut(s) 209, 281, 484, 505, 616, 623
FatI CATG 6 cut(s) 205, 277, 480, 501, 612, 619
FauI CCCGC 1 cut(s) 396
FauNDI CATATG 1 cut(s) 370
Fnu4HI GCNGC 5 cut(s) 189, 306, 473, 506, 578
FokI GGATG 1 cut(s) 345
Fsp4HI GCNGC 5 cut(s) 189, 306, 473, 506, 578
FspBI CTAG 4 cut(s) 9, 141, 309, 518
GlaI GCGC 1 cut(s) 585
GluI GCNGC 5 cut(s) 189, 306, 473, 506, 578
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 5 cut(s) 111, 210, 343, 580, 604
HapII CCGG 2 cut(s) 287, 340
HgaI GACGC 2 cut(s) 80, 263
HhaI GCGC 1 cut(s) 586
Hin1II CATG 6 cut(s) 209, 281, 484, 505, 616, 623
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HincII GTYRAC 2 cut(s) 47, 446
HindII GTYRAC 2 cut(s) 47, 446
HinfI GANTC 2 cut(s) 167, 260
HpaII CCGG 2 cut(s) 287, 340
Hpy166II GTNNAC 2 cut(s) 47, 446
Hpy188I TCNGA 3 cut(s) 29, 184, 609
Hpy188III TCNNGA 2 cut(s) 98, 481
Hpy8I GTNNAC 2 cut(s) 47, 446
HpyCH4III ACNGT 2 cut(s) 238, 531
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 9 cut(s) 23, 205, 248, 368, 397, 472, 539, 616, 623
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 185, 197, 349, 427, 463, 472, 583
HpyF3I CTNAG 2 cut(s) 105, 327
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 6 cut(s) 209, 281, 484, 505, 616, 623
HspAI GCGC 1 cut(s) 584
KroI GCCGGC 1 cut(s) 339
KroNI GCCGGC 1 cut(s) 341
LmnI GCTCC 5 cut(s) 22, 29, 271, 496, 526
Lsp1109I GCAGC 2 cut(s) 292, 484
LweI GCATC 5 cut(s) 175, 188, 367, 444, 632
MaeI CTAG 4 cut(s) 9, 141, 309, 518
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 151
MlsI TGGCCA 1 cut(s) 210
MluCI AATT 1 cut(s) 144
MluNI TGGCCA 1 cut(s) 210
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 7
Mox20I TGGCCA 1 cut(s) 210
Mph1103I ATGCAT 3 cut(s) 207, 250, 625
MroNI GCCGGC 1 cut(s) 339
MscI TGGCCA 1 cut(s) 210
MseI TTAA 1 cut(s) 648
Msp20I TGGCCA 1 cut(s) 210
MspI CCGG 2 cut(s) 287, 340
MwoI GCNNNNNNNGC 8 cut(s) 29, 185, 197, 349, 427, 463, 472, 583
NaeI GCCGGC 1 cut(s) 341
NdeI CATATG 1 cut(s) 370
NgoMIV GCCGGC 1 cut(s) 339
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 6 cut(s) 209, 281, 484, 505, 616, 623
NlaIV GGNNCC 1 cut(s) 31
NsiI ATGCAT 3 cut(s) 207, 250, 625
NspI RCATGY 3 cut(s) 505, 616, 623
PagI TCATGA 1 cut(s) 480
PceI AGGCCT 1 cut(s) 604
PdiI GCCGGC 1 cut(s) 341
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 5 cut(s) 190, 307, 474, 507, 579
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 109
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 5 cut(s) 189, 306, 473, 506, 578
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 1 cut(s) 254
SfaNI GCATC 5 cut(s) 175, 188, 367, 444, 632
SfcI CTRYAG 1 cut(s) 21
Sse9I AATT 1 cut(s) 144
SseBI AGGCCT 1 cut(s) 604
SsiI CCGC 8 cut(s) 88, 188, 362, 389, 464, 506, 577, 665
SspMI CTAG 4 cut(s) 9, 141, 309, 518
StuI AGGCCT 1 cut(s) 604
TaaI ACNGT 2 cut(s) 238, 531
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 99, 195
TaqII GACCGA 1 cut(s) 32
TasI AATT 1 cut(s) 144
TauI GCSGC 3 cut(s) 191, 508, 580
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 1 cut(s) 243
TseI GCWGC 2 cut(s) 305, 472
TspDTI ATGAA 2 cut(s) 469, 497
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 1 cut(s) 243
XceI RCATGY 3 cut(s) 505, 616, 623
XspI CTAG 4 cut(s) 9, 141, 309, 518
Zsp2I ATGCAT 3 cut(s) 207, 250, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.