RLG00000028658

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015246
Physical Location & Seq
Forward (+)
25726964 .. 25729556
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028658

Sequence Viewer

Length: 1119 bp
ATGACGACTCGAATCGCTCCGGGAGTCGGAGCCAACTTGCTCGGTCAACACTCCGCCGAGAGAAACCAAGACGCCACTGCTTACGTCGGCAATCTCGACCCTCAGGTTAGTGAAGAATTGCTGTGGGAGCTGTTTGTTCAAGCCGGCCCTGTTGTTAATGTGTATGTTCCCAAAGATAGAGTGACAAATCTGCATCAAGGATACGGATTCGTGGAGTTTCGAAGCGAAGAGGATGCTGATTATGCAATCAAGGTGCTCAATATGATCAAGCTTTATGGAAAGCCAATTCGAGTAAATAAGGCTTCCCAAGATAAAAAGAGCTTGGATGTAGGGGCAAACCTTTTCGTCGGAAATCTTGATTCTGATGTGGATGAGAAACTTCTCTATGATACTTTCAGTGCATTTGGAGTCATAGTTACAAACCCAAAGATAATGAGAGACCCTGAAACTGGGAACTCCCGTGGATTTGGCTTCATTAGCTATGATTCCTTCGAGGCATCTGATGCAGCTATTGAGGCAATGAATGGCCAGTATCTGTGCAATCGTCAAATAACAGTCTCATATGCATATAAGAAAGACACTAAAGGGGAGCGTCATGGTACTCCGGCAGAGAGAGTTTTGGCTGCTAGCAATCCTACATCTAAGAGCCGGCCTCACACACTGTTTGCTAGTGGGCCTCCAACACTTCCCAATGGTTCTCAGGCCAATGGTACCATGGGTGCTCCAGTGCCTCTGCGTCCCTTTGCAAATGGCGGTGTTGTTCCAGGTCTGATTCCGCCTCTTCGTCCACAACCCCCCCAAGGTATGGCTTTCCCGCCTATGCAGGTGGCTGTTCAACCAACTTGGCAAGGTCAGCCTCAACAACCAGGTCAACCAATGATGCCTCCACCGCCAATGCAGCAGTTCAGACCTGCTCCCCCAAACATGCCGCCACCCCCTCCACAGGCAGCTCCAGCTCCTCCACGGCCGCTTCCACCTCCTATGGCTATGGGCAACCAACAACAGATGTGGCGGCCACCACCTCCCCCTCAAATGAGGCCTCCTAACATGCAACACGCATCAATGCCGCCACCTCCGCCACTTAACAACCCACCCCTACCGCCGCCACCTATGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

373

Amino Acids

40.35

Weight (kDa)

8.54

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 28 - 97 4.5e-20 RNA recognition motif
RRM_1 PF00076 114 - 185 1.6e-20 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18510
fragaria_vesca FvH4_7g10600
malus_domestica MD07G1111200.v1.1
prunus_persica Prupe.2G134600_v2.0.a1
pyrus_communis pycom07g09600
rosa_chinensis RchiOBHm_Chr1g0348441
rosa_laevigata RLG00000016689 RLG00000028645 RLG00000028653 RLG00000028658
rosa_multiflora Rmu_sc0000091.1_g000014
rosa_roxburghii Rroxscaffold_4G00306980
rosa_rugosa Rorug01G0194600
rosa_samantha Rh1BG179000 Rh1CG197000
rosa_wichuraiana Rw1G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 814
Acc36I ACCTGC 2 cut(s) 814, 919
Acc65I GGTACC 1 cut(s) 710
AccB1I GGYRCC 1 cut(s) 710
AccB7I CCANNNNNTGG 1 cut(s) 805
AcoI YGGCCR 3 cut(s) 526, 965, 1013
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 601, 712
AfiI CCNNNNNNNGG 5 cut(s) 26, 449, 800, 805, 943
AgsI TTSAA 2 cut(s) 140, 836
AjnI CCWGG 2 cut(s) 763, 865
AluBI AGCT 7 cut(s) 130, 271, 321, 480, 509, 950, 956
AluI AGCT 7 cut(s) 130, 271, 321, 480, 509, 950, 956
Alw21I GWGCWC 2 cut(s) 258, 724
Alw26I GTCTC 2 cut(s) 432, 562
AlwNI CAGNNNCTG 1 cut(s) 535
AoxI GGCC 8 cut(s) 145, 526, 650, 674, 702, 965, 1013, 1037
ApeKI GCWGC 4 cut(s) 506, 623, 898, 947
Asp718I GGTACC 1 cut(s) 710
AspS9I GGNCC 2 cut(s) 146, 674
AsuC2I CCSGG 1 cut(s) 21
AsuII TTCGAA 1 cut(s) 220
AsuNHI GCTAGC 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 102
BalI TGGCCA 1 cut(s) 528
BanI GGYRCC 1 cut(s) 710
Bbv12I GWGCWC 2 cut(s) 258, 724
BbvI GCAGC 4 cut(s) 518, 610, 910, 959
BceAI ACGGC 1 cut(s) 980
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 765, 867
BciVI GTATCC 1 cut(s) 194
BclI TGATCA 1 cut(s) 264
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 2 cut(s) 432, 562
BfaI CTAG 2 cut(s) 627, 669
BfuAI ACCTGC 2 cut(s) 814, 919
BfuI GTATCC 1 cut(s) 194
BisI GCNGC 9 cut(s) 507, 624, 899, 929, 948, 968, 1013, 1067, 1103
BlsI GCNGC 9 cut(s) 508, 625, 900, 930, 949, 969, 1014, 1068, 1104
Bme1390I CCNGG 3 cut(s) 21, 765, 867
BmgT120I GGNCC 2 cut(s) 146, 674
BmiI GGNNCC 2 cut(s) 31, 712
BmrFI CCNGG 3 cut(s) 21, 765, 867
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 6 cut(s) 202, 223, 493, 506, 870, 1067
BmtI GCTAGC 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 459
BplI GAGNNNNNCTC 2 cut(s) 637, 669
BpmI CTGGAG 2 cut(s) 708, 936
Bpu14I TTCGAA 1 cut(s) 220
BpuMI CCSGG 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 432
BsaJI CCNNGG 4 cut(s) 460, 714, 799, 962
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 449, 800, 805, 943
Bse118I RCCGGY 2 cut(s) 143, 648
Bse1I ACTGG 3 cut(s) 454, 529, 725
Bse21I CCTNAGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 2 cut(s) 765, 867
BseDI CCNNGG 4 cut(s) 460, 714, 799, 962
BseGI GGATG 3 cut(s) 238, 331, 376
BseLI CCNNNNNNNGG 5 cut(s) 26, 449, 800, 805, 943
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 2 cut(s) 116, 713
BseNI ACTGG 3 cut(s) 454, 529, 725
BseRI GAGGAG 1 cut(s) 948
BseX3I CGGCCG 1 cut(s) 965
BseXI GCAGC 4 cut(s) 518, 610, 910, 959
Bsh1285I CGRYCG 1 cut(s) 968
BshFI GGCC 8 cut(s) 147, 528, 652, 676, 704, 967, 1015, 1039
BshNI GGYRCC 1 cut(s) 710
BsiEI CGRYCG 1 cut(s) 968
BsiHKAI GWGCWC 2 cut(s) 258, 724
BsiSI CCGG 4 cut(s) 20, 144, 605, 649
BslFI GGGAC 1 cut(s) 723
BslI CCNNNNNNNGG 5 cut(s) 26, 449, 800, 805, 943
BsmAI GTCTC 2 cut(s) 432, 562
BsmFI GGGAC 1 cut(s) 723
BsnI GGCC 8 cut(s) 147, 528, 652, 676, 704, 967, 1015, 1039
Bso31I GGTCTC 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 220
Bsp1286I GDGCHC 2 cut(s) 258, 724
Bsp143I GATC 1 cut(s) 264
Bsp19I CCATGG 1 cut(s) 714
BspANI GGCC 8 cut(s) 147, 528, 652, 676, 704, 967, 1015, 1039
BspCNI CTCAG 2 cut(s) 115, 712
BspLI GGNNCC 2 cut(s) 31, 712
BspMI ACCTGC 2 cut(s) 814, 919
BspOI GCTAGC 1 cut(s) 630
BspT104I TTCGAA 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 2 cut(s) 143, 648
BsrI ACTGG 3 cut(s) 454, 529, 725
BssAI RCCGGY 2 cut(s) 143, 648
BssECI CCNNGG 4 cut(s) 460, 714, 799, 962
BssMI GATC 1 cut(s) 264
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 714, 799
Bst2UI CCWGG 2 cut(s) 765, 867
Bst4CI ACNGT 2 cut(s) 556, 663
Bst6I CTCTTC 2 cut(s) 222, 786
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 503
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 3 cut(s) 145, 628, 650
BstDEI CTNAG 3 cut(s) 102, 642, 699
BstDSI CCRYGG 3 cut(s) 460, 714, 962
BstF5I GGATG 3 cut(s) 238, 331, 376
BstKTI GATC 1 cut(s) 267
BstMAI GTCTC 2 cut(s) 432, 562
BstMBI GATC 1 cut(s) 264
BstMCI CGRYCG 1 cut(s) 968
BstNI CCWGG 2 cut(s) 765, 867
BstNSI RCATGY 2 cut(s) 928, 1051
BstSCI CCNGG 3 cut(s) 19, 763, 865
BstV1I GCAGC 4 cut(s) 518, 610, 910, 959
BstZI CGGCCG 1 cut(s) 965
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 8 cut(s) 147, 528, 652, 676, 704, 967, 1015, 1039
BtgI CCRYGG 3 cut(s) 460, 714, 962
BtsCI GGATG 3 cut(s) 238, 331, 376
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 4 cut(s) 75, 403, 659, 732
BveI ACCTGC 2 cut(s) 814, 919
Cac8I GCNNGC 3 cut(s) 145, 628, 650
CaiI CAGNNNCTG 1 cut(s) 535
Cfr10I RCCGGY 2 cut(s) 143, 648
Cfr13I GGNCC 2 cut(s) 146, 674
CseI GACGC 3 cut(s) 80, 581, 725
CsiI ACCWGGT 1 cut(s) 865
Csp6I GTAC 2 cut(s) 600, 711
CspCI CAANNNNNGTGG 2 cut(s) 989, 1024
CviAII CATG 4 cut(s) 596, 715, 925, 1048
CviQI GTAC 2 cut(s) 600, 711
DdeI CTNAG 3 cut(s) 102, 642, 699
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
EaeI YGGCCR 3 cut(s) 526, 965, 1013
EagI CGGCCG 1 cut(s) 965
Eam1104I CTCTTC 2 cut(s) 222, 786
EarI CTCTTC 2 cut(s) 222, 786
EciI GGCGGA 3 cut(s) 43, 765, 1065
EclXI CGGCCG 1 cut(s) 965
Eco130I CCWWGG 2 cut(s) 714, 799
Eco147I AGGCCT 1 cut(s) 1039
Eco31I GGTCTC 1 cut(s) 432
Eco52I CGGCCG 1 cut(s) 965
Eco81I CCTNAGG 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 763, 865
EcoT14I CCWWGG 2 cut(s) 714, 799
EcoT22I ATGCAT 1 cut(s) 568
ErhI CCWWGG 2 cut(s) 714, 799
FaeI CATG 4 cut(s) 599, 718, 928, 1051
FaqI GGGAC 1 cut(s) 723
FatI CATG 4 cut(s) 595, 714, 924, 1047
FauI CCCGC 1 cut(s) 822
FauNDI CATATG 1 cut(s) 562
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 9 cut(s) 507, 624, 899, 929, 948, 968, 1013, 1067, 1103
FokI GGATG 3 cut(s) 245, 338, 383
Fsp4HI GCNGC 9 cut(s) 507, 624, 899, 929, 948, 968, 1013, 1067, 1103
FspBI CTAG 2 cut(s) 627, 669
GluI GCNGC 9 cut(s) 507, 624, 899, 929, 948, 968, 1013, 1067, 1103
GsuI CTGGAG 2 cut(s) 708, 936
HaeIII GGCC 8 cut(s) 147, 528, 652, 676, 704, 967, 1015, 1039
HapII CCGG 4 cut(s) 20, 144, 605, 649
HgaI GACGC 3 cut(s) 80, 581, 725
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 4 cut(s) 599, 718, 928, 1051
HincII GTYRAC 2 cut(s) 47, 872
HindII GTYRAC 2 cut(s) 47, 872
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 8 cut(s) 7, 12, 24, 207, 359, 408, 485, 772
HpaII CCGG 4 cut(s) 20, 144, 605, 649
Hpy166II GTNNAC 3 cut(s) 47, 788, 872
Hpy188I TCNGA 6 cut(s) 29, 350, 364, 502, 771, 908
Hpy188III TCNNGA 2 cut(s) 95, 356
Hpy8I GTNNAC 3 cut(s) 47, 788, 872
Hpy99I CGWCG 2 cut(s) 89, 350
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 2 cut(s) 556, 663
HpyCH4IV ACGT 1 cut(s) 84
HpyF3I CTNAG 3 cut(s) 102, 642, 699
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 4 cut(s) 599, 718, 928, 1051
KpnI GGTACC 1 cut(s) 714
KroI GCCGGC 2 cut(s) 143, 648
KroNI GCCGGC 2 cut(s) 145, 650
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 8 cut(s) 22, 29, 127, 589, 727, 919, 955, 961
Lsp1109I GCAGC 4 cut(s) 518, 610, 910, 959
LweI GCATC 6 cut(s) 202, 223, 493, 506, 870, 1067
MabI ACCWGGT 1 cut(s) 865
MaeI CTAG 2 cut(s) 627, 669
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 2 cut(s) 181, 415
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MboII GAAGA 3 cut(s) 125, 239, 773
MhlI GDGCHC 2 cut(s) 258, 724
MlsI TGGCCA 1 cut(s) 528
MluCI AATT 2 cut(s) 116, 285
MluNI TGGCCA 1 cut(s) 528
MlyI GAGTC 2 cut(s) 33, 417
MmeI TCCRAC 3 cut(s) 7, 328, 704
Mox20I TGGCCA 1 cut(s) 528
Mph1103I ATGCAT 1 cut(s) 568
MroNI GCCGGC 2 cut(s) 143, 648
MscI TGGCCA 1 cut(s) 528
MseI TTAA 2 cut(s) 156, 1083
Msp20I TGGCCA 1 cut(s) 528
MspI CCGG 4 cut(s) 20, 144, 605, 649
MspR9I CCNGG 3 cut(s) 21, 765, 867
MvaI CCWGG 2 cut(s) 765, 867
NaeI GCCGGC 2 cut(s) 145, 650
NciI CCSGG 1 cut(s) 21
NcoI CCATGG 1 cut(s) 714
NdeI CATATG 1 cut(s) 562
NdeII GATC 1 cut(s) 264
NgoMIV GCCGGC 2 cut(s) 143, 648
NheI GCTAGC 1 cut(s) 626
NlaIII CATG 4 cut(s) 599, 718, 928, 1051
NlaIV GGNNCC 2 cut(s) 31, 712
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 568
NspI RCATGY 2 cut(s) 928, 1051
NspV TTCGAA 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 814
PceI AGGCCT 1 cut(s) 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PdiI GCCGGC 2 cut(s) 145, 650
PfeI GAWTC 5 cut(s) 12, 207, 359, 485, 772
PflMI CCANNNNNTGG 1 cut(s) 805
PfoI TCCNGGA 1 cut(s) 19
PkrI GCNGC 9 cut(s) 508, 625, 900, 930, 949, 969, 1014, 1068, 1104
PleI GAGTC 2 cut(s) 32, 416
PpsI GAGTC 2 cut(s) 32, 416
Psp6I CCWGG 2 cut(s) 763, 865
PspGI CCWGG 2 cut(s) 763, 865
PspN4I GGNNCC 2 cut(s) 31, 712
PspPI GGNCC 2 cut(s) 146, 674
PstNI CAGNNNCTG 1 cut(s) 535
RsaI GTAC 2 cut(s) 601, 712
RsaNI GTAC 2 cut(s) 600, 711
SaqAI TTAA 2 cut(s) 156, 1083
SatI GCNGC 9 cut(s) 507, 624, 899, 929, 948, 968, 1013, 1067, 1103
Sau3AI GATC 1 cut(s) 264
Sau96I GGNCC 2 cut(s) 146, 674
SchI GAGTC 2 cut(s) 33, 417
ScrFI CCNGG 3 cut(s) 21, 765, 867
SduI GDGCHC 2 cut(s) 258, 724
SexAI ACCWGGT 1 cut(s) 865
SfaNI GCATC 6 cut(s) 202, 223, 493, 506, 870, 1067
SfuI TTCGAA 1 cut(s) 220
Sse9I AATT 2 cut(s) 116, 285
SseBI AGGCCT 1 cut(s) 1039
SspMI CTAG 2 cut(s) 627, 669
StuI AGGCCT 1 cut(s) 1039
StyD4I CCNGG 3 cut(s) 19, 763, 865
StyI CCWWGG 2 cut(s) 714, 799
TaaI ACNGT 2 cut(s) 556, 663
TaiI ACGT 1 cut(s) 87
TaqI TCGA 5 cut(s) 10, 96, 220, 289, 492
TaqII GACCGA 1 cut(s) 32
TasI AATT 2 cut(s) 116, 285
TauI GCSGC 5 cut(s) 931, 970, 1015, 1069, 1105
TfiI GAWTC 5 cut(s) 12, 207, 359, 485, 772
Tru1I TTAA 2 cut(s) 156, 1083
Tru9I TTAA 2 cut(s) 156, 1083
TscAI CASTG 4 cut(s) 82, 403, 666, 732
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 4 cut(s) 506, 623, 898, 947
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 2 cut(s) 463, 536
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 4 cut(s) 82, 403, 666, 732
Van91I CCANNNNNTGG 1 cut(s) 805
XceI RCATGY 2 cut(s) 928, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XspI CTAG 2 cut(s) 627, 669
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.