RLG00000016841

Rho (Ras homology) subfamily of Ras-like small GTPases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004170
Physical Location & Seq
Reverse (-)
10546276 .. 10547946
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016841

Sequence Viewer

Length: 1671 bp
ATGGGCTCTAACGGCAGCAAACAACAATCTGCAGATCATTATACCTTGCTGGGTTTAAGCGATCTAAGAGAAGCTGCCACTCCAGAACAGATTAGAAAGGCCTATCTGAAGAGCGCCTTGAAATATCATCCTGACAAGCTCACCTTCCTTATTCTCGCCGAGCAAAGCGAAGCGGCCAGAAAAGCCAAGAAGGAGGAGATCGACACCCACTTCAAGAAAATCGTAGCAGCATATGAGGTATTGAGCGATCCGGTCGAGAGACGAAACTATGACTTAGGACGGATCTATAGTTCCTCCAGCTCCGGTTGTCATACTAGTACTGATAGTAGTACTAGTACTCCTACTGAAGATCATCATCATCAGCATCAGCGTCATGATGTGGATGATGAAATCCCTAGTGACTGTGCTCCGGGGGATTTCTTCAAGGTGTTTGGTCCTGCTTTCGAAAGAAACGCGCGGTGGTCGGCTAAGAAACCGGTGCCGGGTTTAGGGAACGAGAGAACTCCTATGGAGGAAGTGGACAAGTTCTATGAGTTTTGGTACAGCTTCAAAAGCAGCAGAGACTTCACAGGTAAGTATCAGTATAAACGTGACAGAGAGTGGGCTATGAGGCAGGAGTATATACGAATTCGGAGACTCGCTGACAATGCCTATGGGAAAGACCCGAGGATATTGAGGAGGAAGGAAGAGGAGAAAGCCGAGAAGGAAAGGAAAAAGATGGAAAAGAAAATGAGGAAGGAAGCAGAGGCTAGGGCGGCTGAGGAGGAGAGGCGGCGGAGGGAGGAGGAGGAGAAGCGAGCTGCGGAAGTGGCTTTGGAGCAGAAGAAGGTTAAGGAGGGGGAGAGGAAACTCTTGAAGAAGGAAAGGAGTCGTTTTCGAACACTCTCAGTAGGGTTGCATGGTCTTGAGAAGGATGATGTTGAAAGGCTTTGCACGTGGTTTGATTTTGAAAAGCTTAGGAATGTATGTGAAAGAATTGAAGACGGAAAAGATGAGGGGCTAATGGACCGGTTGGCAAAAATTGTTAGCGAGATCAAGAGAGAAGAAGATGAGAATAAGAAAAGTAGTACTCAACAACAAACACAGAACGAGAATGAGAGTAATGGTAATGTCAATGGTAGTGTTAAGAAGGAGAGACCTTGGGGGAAAGAAGAGATTGAGCTATTGAGGAAAGGGATGATGAAATTTCCGAAAGGAACACCGCGAAGGTGGGAAGTGGTTTCGGAGTACATTGGTACCGGAAGATCCGTGGAGGAAGTTCTCAAGGCAATGAAGGCAGTGCTTCTCAAGAAACCTGATGATAGCAAAGCGTTTGACTATTTTCTTGAGAAGAGGAAGCCTTCGAAATCTATTGACTCGCCGCTCACAACAAGAATAGAAGTAGAAGGGGTATCTATGTCATCGACTAGTACTAGTACGAGTCGTCCTCCTCCCAATGATCAAAAGTCTGCAAGTGGATCAACGTCGAGTAGTTCGGAGCAGGATGATGTGTGGTCTGTTGAGCAAGAAAGGGCGCTGATTCAGGCAATGAAGACTGTTCCGAAGGAACTATCCGGCGGTGCGAGGTGGGAGCGAATTGCTGCTGTTGTTCCTCAGAAGACCGCAAATCAATGCAAGAAAAAGTTTGCATTGCTCAAGGAAAACTTGAGAAGCAAAAAGAGTTCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632 Ras

Protein Analysis

557

Amino Acids

64.12

Weight (kDa)

9.21

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 12 - 91 3.6e-15 DnaJ domain
ZUO1-like_ZHD PF21884 139 - 198 1.1e-19 Zuotin-like, zuotin homology domain
Myb_DNA-binding_2 PF23082 379 - 448 5e-28 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 497 - 542 6.5e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1235
AccB1I GGYRCC 2 cut(s) 478, 1235
AccBSI CCGCTC 1 cut(s) 1363
AccII CGCG 3 cut(s) 455, 457, 1204
AclWI GGATC 4 cut(s) 242, 290, 1239, 1465
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 2 cut(s) 627, 1184
AcuI CTGAAG 2 cut(s) 128, 366
AcvI CACGTG 1 cut(s) 936
AfaI GTAC 9 cut(s) 319, 331, 337, 542, 1069, 1229, 1237, 1411, 1417
AfiI CCNNNNNNNGG 2 cut(s) 482, 488
AgeI ACCGGT 2 cut(s) 475, 1008
AgsI TTSAA 8 cut(s) 121, 214, 424, 550, 856, 923, 950, 980
AhlI ACTAGT 4 cut(s) 314, 332, 1406, 1412
AloI GAACNNNNNNTCC 2 cut(s) 274, 306
AluBI AGCT 7 cut(s) 74, 139, 300, 546, 800, 955, 1162
AluI AGCT 7 cut(s) 74, 139, 300, 546, 800, 955, 1162
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 4 cut(s) 253, 555, 628, 1129
AlwI GGATC 4 cut(s) 242, 290, 1239, 1465
Ama87I CYCGRG 1 cut(s) 664
AoxI GGCC 2 cut(s) 99, 174
ApeKI GCWGC 6 cut(s) 15, 74, 227, 555, 800, 1580
ApoI RAATTY 2 cut(s) 627, 1184
AsiGI ACCGGT 2 cut(s) 475, 1008
Asp718I GGTACC 1 cut(s) 1235
AspLEI GCGC 3 cut(s) 116, 457, 1516
AspS9I GGNCC 2 cut(s) 434, 1006
AsuC2I CCSGG 2 cut(s) 411, 483
AsuHPI GGTGA 1 cut(s) 133
AsuII TTCGAA 3 cut(s) 444, 877, 1343
AvaI CYCGRG 1 cut(s) 664
AvaII GGWCC 2 cut(s) 434, 1006
BaeI ACNNNNGTAYC 2 cut(s) 1219, 1252
BanI GGYRCC 2 cut(s) 478, 1235
BanII GRGCYC 1 cut(s) 8
BbrPI CACGTG 1 cut(s) 936
BbsI GAAGAC 3 cut(s) 987, 1538, 1604
Bbv12I GWGCWC 1 cut(s) 409
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 6 cut(s) 27, 61, 239, 567, 787, 1567
BccI CCATC 1 cut(s) 712
BceAI ACGGC 1 cut(s) 28
BclI TGATCA 1 cut(s) 1438
BcnI CCSGG 2 cut(s) 411, 483
BcoDI GTCTC 4 cut(s) 253, 555, 628, 1129
BcuI ACTAGT 4 cut(s) 314, 332, 1406, 1412
BfaI CTAG 6 cut(s) 315, 333, 396, 750, 1407, 1413
BfmI CTRYAG 2 cut(s) 30, 286
BfoI RGCGCY 2 cut(s) 117, 1517
BmcAI AGTACT 5 cut(s) 319, 331, 337, 1069, 1411
Bme1390I CCNGG 2 cut(s) 411, 483
Bme18I GGWCC 2 cut(s) 434, 1006
BmeT110I CYCGRG 1 cut(s) 664
BmgT120I GGNCC 2 cut(s) 434, 1006
BmiI GGNNCC 2 cut(s) 480, 1237
BmrFI CCNGG 2 cut(s) 411, 483
BmsI GCATC 1 cut(s) 373
BpiI GAAGAC 3 cut(s) 987, 1538, 1604
BplI GAGNNNNNCTC 2 cut(s) 1411, 1443
BpmI CTGGAG 2 cut(s) 66, 280
Bpu10I CCTNAGC 2 cut(s) 759, 956
Bpu14I TTCGAA 3 cut(s) 444, 877, 1343
BpuEI CTTGAG 6 cut(s) 926, 1247, 1271, 1346, 1619, 1666
BpuMI CCSGG 2 cut(s) 411, 483
BsaAI YACGTR 1 cut(s) 936
BsaBI GATNNNNATC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 1129
BsaJI CCNNGG 4 cut(s) 410, 665, 1139, 1248
BsaWI WCCGGW 5 cut(s) 250, 302, 475, 1008, 1238
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 482, 488
Bse118I RCCGGY 2 cut(s) 475, 1008
Bse3DI GCAATG 3 cut(s) 1275, 1533, 1628
Bse8I GATNNNNATC 1 cut(s) 354
BseDI CCNNGG 4 cut(s) 410, 665, 1139, 1248
BseGI GGATG 5 cut(s) 127, 388, 919, 1182, 1489
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 2 cut(s) 482, 488
BseMI GCAATG 3 cut(s) 1275, 1533, 1628
BseMII CTCAG 3 cut(s) 750, 900, 1607
BseRI GAGGAG 9 cut(s) 209, 691, 704, 776, 779, 797, 800, 803, 1419
BseXI GCAGC 6 cut(s) 27, 61, 239, 567, 787, 1567
BseYI CCCAGC 1 cut(s) 49
Bsh1236I CGCG 3 cut(s) 455, 457, 1204
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 2 cut(s) 101, 176
BshNI GGYRCC 2 cut(s) 478, 1235
BshTI ACCGGT 2 cut(s) 475, 1008
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 664
BsiSI CCGG 8 cut(s) 251, 303, 410, 476, 482, 1009, 1239, 1554
BslI CCNNNNNNNGG 2 cut(s) 482, 488
BsmAI GTCTC 4 cut(s) 253, 555, 628, 1129
BsmBI CGTCTC 1 cut(s) 253
BsnI GGCC 2 cut(s) 101, 176
Bso31I GGTCTC 1 cut(s) 1129
BsoBI CYCGRG 1 cut(s) 664
Bsp119I TTCGAA 3 cut(s) 444, 877, 1343
Bsp1286I GDGCHC 2 cut(s) 8, 409
BspANI GGCC 2 cut(s) 101, 176
BspCNI CTCAG 3 cut(s) 751, 899, 1606
BspFNI CGCG 3 cut(s) 455, 457, 1204
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 2 cut(s) 480, 1237
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 4 cut(s) 242, 290, 1239, 1465
BspQI GCTCTTC 1 cut(s) 104
BspT104I TTCGAA 3 cut(s) 444, 877, 1343
BspT107I GGYRCC 2 cut(s) 478, 1235
BspTNI GGTCTC 1 cut(s) 1129
BsrBI CCGCTC 1 cut(s) 1363
BsrDI GCAATG 3 cut(s) 1275, 1533, 1628
BsrFI RCCGGY 2 cut(s) 475, 1008
BssAI RCCGGY 2 cut(s) 475, 1008
BssECI CCNNGG 4 cut(s) 410, 665, 1139, 1248
BssT1I CCWWGG 1 cut(s) 1139
Bst4CI ACNGT 2 cut(s) 404, 1537
Bst6I CTCTTC 4 cut(s) 104, 681, 1146, 1325
BstBAI YACGTR 1 cut(s) 936
BstBI TTCGAA 3 cut(s) 444, 877, 1343
BstC8I GCNNGC 1 cut(s) 798
BstDEI CTNAG 7 cut(s) 65, 274, 468, 759, 886, 956, 1593
BstDSI CCRYGG 1 cut(s) 1248
BstF5I GGATG 5 cut(s) 127, 388, 919, 1182, 1489
BstFNI CGCG 3 cut(s) 455, 457, 1204
BstH2I RGCGCY 2 cut(s) 117, 1517
BstHHI GCGC 3 cut(s) 116, 457, 1516
BstMAI GTCTC 4 cut(s) 253, 555, 628, 1129
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 7 cut(s) 12, 182, 552, 647, 755, 809, 1274
BstSCI CCNGG 2 cut(s) 409, 481
BstSFI CTRYAG 2 cut(s) 30, 286
BstUI CGCG 3 cut(s) 455, 457, 1204
BstV1I GCAGC 6 cut(s) 27, 61, 239, 567, 787, 1567
BstV2I GAAGAC 3 cut(s) 987, 1538, 1604
BstX2I RGATCY 2 cut(s) 282, 1244
BstYI RGATCY 2 cut(s) 282, 1244
BsuRI GGCC 2 cut(s) 101, 176
BtgI CCRYGG 1 cut(s) 1248
BtsCI GGATG 5 cut(s) 127, 388, 919, 1182, 1489
BtsI GCAGTG 1 cut(s) 1284
BtsIMutI CAGTG 1 cut(s) 1284
Cac8I GCNNGC 1 cut(s) 798
CciI TCATGA 1 cut(s) 373
CfoI GCGC 3 cut(s) 116, 457, 1516
Cfr10I RCCGGY 2 cut(s) 475, 1008
Cfr13I GGNCC 2 cut(s) 434, 1006
CseI GACGC 1 cut(s) 359
Csp6I GTAC 9 cut(s) 318, 330, 336, 541, 1068, 1228, 1236, 1410, 1416
CspAI ACCGGT 2 cut(s) 475, 1008
CviAII CATG 2 cut(s) 374, 899
CviQI GTAC 9 cut(s) 318, 330, 336, 541, 1068, 1228, 1236, 1410, 1416
DdeI CTNAG 7 cut(s) 65, 274, 468, 759, 886, 956, 1593
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 4 cut(s) 104, 681, 1146, 1325
EarI CTCTTC 4 cut(s) 104, 681, 1146, 1325
EciI GGCGGA 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 1139
Eco147I AGGCCT 1 cut(s) 101
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 1129
Eco47I GGWCC 2 cut(s) 434, 1006
Eco57I CTGAAG 2 cut(s) 128, 366
Eco72I CACGTG 1 cut(s) 936
Eco88I CYCGRG 1 cut(s) 664
EcoRI GAATTC 1 cut(s) 627
EcoT14I CCWWGG 1 cut(s) 1139
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 1139
Esp3I CGTCTC 1 cut(s) 253
FaeI CATG 2 cut(s) 377, 902
FalI AAGNNNNNCTT 6 cut(s) 101, 133, 128, 160, 1628, 1660
FatI CATG 2 cut(s) 373, 898
FauNDI CATATG 1 cut(s) 232
FbaI TGATCA 1 cut(s) 1438
FokI GGATG 5 cut(s) 114, 395, 926, 1189, 1496
FriOI GRGCYC 1 cut(s) 8
FspBI CTAG 6 cut(s) 315, 333, 396, 750, 1407, 1413
GlaI GCGC 3 cut(s) 115, 456, 1515
GsaI CCCAGC 1 cut(s) 53
GsuI CTGGAG 2 cut(s) 66, 280
HaeII RGCGCY 2 cut(s) 117, 1517
HaeIII GGCC 2 cut(s) 101, 176
HapII CCGG 8 cut(s) 251, 303, 410, 476, 482, 1009, 1239, 1554
HgaI GACGC 1 cut(s) 359
HhaI GCGC 3 cut(s) 116, 457, 1516
Hin1II CATG 2 cut(s) 377, 902
Hin6I GCGC 3 cut(s) 114, 455, 1514
HinP1I GCGC 3 cut(s) 114, 455, 1514
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 5 cut(s) 636, 868, 1355, 1420, 1519
HpaII CCGG 8 cut(s) 251, 303, 410, 476, 482, 1009, 1239, 1554
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 7 cut(s) 108, 633, 1191, 1225, 1477, 1542, 1596
Hpy8I GTNNAC 1 cut(s) 520
Hpy99I CGWCG 1 cut(s) 1468
HpyCH4III ACNGT 2 cut(s) 404, 1537
HpyCH4IV ACGT 3 cut(s) 589, 935, 1463
HpyCH4V TGCA 6 cut(s) 32, 898, 933, 1451, 1614, 1628
HpyF10VI GCNNNNNNNGC 7 cut(s) 12, 182, 552, 647, 755, 809, 1274
HpyF3I CTNAG 7 cut(s) 65, 274, 468, 759, 886, 956, 1593
HpySE526I ACGT 3 cut(s) 589, 935, 1463
Hsp92II CATG 2 cut(s) 377, 902
HspAI GCGC 3 cut(s) 114, 455, 1514
KpnI GGTACC 1 cut(s) 1239
Ksp22I TGATCA 1 cut(s) 1438
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 5 cut(s) 305, 412, 817, 1477, 1570
Lsp1109I GCAGC 6 cut(s) 27, 61, 239, 567, 787, 1567
LweI GCATC 1 cut(s) 373
MaeI CTAG 6 cut(s) 315, 333, 396, 750, 1407, 1413
MaeII ACGT 3 cut(s) 589, 935, 1463
MaeIII GTNAC 2 cut(s) 398, 590
MbiI CCGCTC 1 cut(s) 1363
MflI RGATCY 2 cut(s) 282, 1244
MhlI GDGCHC 2 cut(s) 8, 409
MluCI AATT 5 cut(s) 627, 975, 1020, 1184, 1575
MlyI GAGTC 4 cut(s) 630, 877, 1349, 1429
MseI TTAA 3 cut(s) 56, 831, 1125
MspI CCGG 8 cut(s) 251, 303, 410, 476, 482, 1009, 1239, 1554
MspR9I CCNGG 2 cut(s) 411, 483
MvnI CGCG 3 cut(s) 455, 457, 1204
MwoI GCNNNNNNNGC 7 cut(s) 12, 182, 552, 647, 755, 809, 1274
NciI CCSGG 2 cut(s) 411, 483
NdeI CATATG 1 cut(s) 232
NlaIII CATG 2 cut(s) 377, 902
NlaIV GGNNCC 2 cut(s) 480, 1237
NmeAIII GCCGAG 2 cut(s) 184, 724
NmuCI GTSAC 2 cut(s) 398, 590
NspV TTCGAA 3 cut(s) 444, 877, 1343
PagI TCATGA 1 cut(s) 373
PceI AGGCCT 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 1519
PinAI ACCGGT 2 cut(s) 475, 1008
PleI GAGTC 4 cut(s) 630, 876, 1349, 1428
PmaCI CACGTG 1 cut(s) 936
PmlI CACGTG 1 cut(s) 936
PpsI GAGTC 4 cut(s) 630, 876, 1349, 1428
Ppu21I YACGTR 1 cut(s) 936
PspCI CACGTG 1 cut(s) 936
PspFI CCCAGC 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 480, 1237
PspPI GGNCC 2 cut(s) 434, 1006
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 2 cut(s) 282, 1244
RsaI GTAC 9 cut(s) 319, 331, 337, 542, 1069, 1229, 1237, 1411, 1417
RsaNI GTAC 9 cut(s) 318, 330, 336, 541, 1068, 1228, 1236, 1410, 1416
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 3 cut(s) 56, 831, 1125
Sau96I GGNCC 2 cut(s) 434, 1006
ScaI AGTACT 5 cut(s) 319, 331, 337, 1069, 1411
SchI GAGTC 4 cut(s) 630, 877, 1349, 1429
ScrFI CCNGG 2 cut(s) 411, 483
SduI GDGCHC 2 cut(s) 8, 409
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 2 cut(s) 30, 286
SfuI TTCGAA 3 cut(s) 444, 877, 1343
SinI GGWCC 2 cut(s) 434, 1006
SmlI CTYRAG 6 cut(s) 905, 1262, 1286, 1325, 1634, 1645
SmoI CTYRAG 6 cut(s) 905, 1262, 1286, 1325, 1634, 1645
SpeI ACTAGT 4 cut(s) 314, 332, 1406, 1412
Sse9I AATT 5 cut(s) 627, 975, 1020, 1184, 1575
SseBI AGGCCT 1 cut(s) 101
SspMI CTAG 6 cut(s) 315, 333, 396, 750, 1407, 1413
StuI AGGCCT 1 cut(s) 101
StyD4I CCNGG 2 cut(s) 409, 481
StyI CCWWGG 1 cut(s) 1139
TaaI ACNGT 2 cut(s) 404, 1537
TaiI ACGT 3 cut(s) 592, 938, 1466
TaqI TCGA 7 cut(s) 201, 255, 444, 877, 1343, 1403, 1466
TasI AATT 5 cut(s) 627, 975, 1020, 1184, 1575
TatI WGTACW 6 cut(s) 317, 329, 335, 1067, 1227, 1409
TauI GCSGC 4 cut(s) 176, 758, 775, 1363
TfiI GAWTC 1 cut(s) 1519
Tru1I TTAA 3 cut(s) 56, 831, 1125
Tru9I TTAA 3 cut(s) 56, 831, 1125
TscAI CASTG 1 cut(s) 1284
TseFI GTSAC 2 cut(s) 398, 590
TseI GCWGC 6 cut(s) 15, 74, 227, 555, 800, 1580
Tsp45I GTSAC 2 cut(s) 398, 590
TspDTI ATGAA 4 cut(s) 402, 1196, 1286, 1544
TspGWI ACGGA 3 cut(s) 295, 999, 1237
TspRI CASTG 1 cut(s) 1284
VpaK11BI GGWCC 2 cut(s) 434, 1006
XapI RAATTY 2 cut(s) 627, 1184
XspI CTAG 6 cut(s) 315, 333, 396, 750, 1407, 1413
ZrmI AGTACT 5 cut(s) 319, 331, 337, 1069, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.