RLG00000017200

Transmembrane family 220, helix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013562
Physical Location & Seq
Forward (+)
14087408 .. 14087934
527 bp
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UTR
Exon/CDS
Intron
RLM00000017200

Sequence Viewer

Length: 435 bp
ATGGCAACACCCAGCAAGCTATACACCTTGTGTTCTCTACTAATGGCAGCACTATTTGCTTACTCAGCTGCTGTTCAATTAAATGACCCTGATTGGTACTTTTGGTTTCCTCTTTATGCTGCTGCTTGTGTTGTAAATCTAGTGAACTGGGCTATCACATCAAAAAAAATCAACCAATTTGCTGAGGCAGCACTATGGCTTGGAATGTTTCTATTTATCAAGGCTGTAGCTGAAGATTTTGTAAATGGTATATCTTGGTTTTGGTCACTGGATTTGAGTGAGAGAGTAATCAGGGAGAAAATAGGAAGTGGGTTGGTCATAATCTCTATGGTTTTGCAGTTGATGGCATCATTATCATCACCAGAAGCTCCCACACAGAAAAGGCCAAGATATCTTGAATATGGTAAAGACCTTAAACCTGCTGGTTTAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM220

Protein Analysis

145

Amino Acids

16.15

Weight (kDa)

7.71

Isoelectric Point (pI)

26.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM220 PF15071 18 - 114 4e-18 Transmembrane family 220, helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013562)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23755
fragaria_vesca FvH4_1g14340 FvH4_1g14340 FvH4_1g14340
malus_domestica MD15G1271500.v1.1
prunus_persica Prupe.7G146700_v2.0.a1 Prupe.7G146700_v2.0.a1
pyrus_communis pycom15g23550
rosa_chinensis RchiOBHm_Chr2g0103011
rosa_laevigata RLG00000017200
rosa_multiflora Rmu_sc0000388.1_g000018
rosa_roxburghii Rroxscaffold_2G00140000
rosa_rugosa Rorug02G0111400
rosa_samantha Rh2AG159600 Rh2BG166100 Rh2CG166300 Rh2DG165000
rosa_wichuraiana Rw2G012470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 427
AcuI CTGAAG 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 30
AfaI GTAC 1 cut(s) 98
AgsI TTSAA 2 cut(s) 77, 398
AluBI AGCT 4 cut(s) 19, 68, 230, 368
AluI AGCT 4 cut(s) 19, 68, 230, 368
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 5 cut(s) 47, 68, 119, 122, 188
AsuHPI GGTGA 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 5 cut(s) 55, 59, 106, 109, 200
BccI CCATC 1 cut(s) 337
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 5 cut(s) 48, 69, 120, 123, 189
BlsI GCNGC 5 cut(s) 49, 70, 121, 124, 190
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 356
BmuI ACTGGG 1 cut(s) 157
Bpu10I CCTNAGC 1 cut(s) 183
Bse1I ACTGG 2 cut(s) 152, 273
BseMII CTCAG 2 cut(s) 78, 174
BseNI ACTGG 2 cut(s) 152, 273
BseXI GCAGC 5 cut(s) 55, 59, 106, 109, 200
BseYI CCCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 385
BsnI GGCC 1 cut(s) 385
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 2 cut(s) 77, 175
BspMI ACCTGC 1 cut(s) 427
BsrI ACTGG 2 cut(s) 152, 273
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 64, 183
BstMWI GCNNNNNNNGC 3 cut(s) 56, 65, 188
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 5 cut(s) 55, 59, 106, 109, 200
BsuRI GGCC 1 cut(s) 385
BtsIMutI CAGTG 1 cut(s) 266
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 17
CaiI CAGNNNCTG 1 cut(s) 71
Csp6I GTAC 1 cut(s) 97
CviJI RGCY 8 cut(s) 19, 68, 152, 199, 224, 230, 368, 385
CviKI_1 RGCY 8 cut(s) 19, 68, 152, 199, 224, 230, 368, 385
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 2 cut(s) 64, 183
DraIII CACNNNGTG 1 cut(s) 30
Eco32I GATATC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 252
EcoRV GATATC 1 cut(s) 392
FaiI YATR 7 cut(s) 22, 117, 196, 251, 320, 329, 402
Fnu4HI GCNGC 5 cut(s) 48, 69, 120, 123, 189
Fsp4HI GCNGC 5 cut(s) 48, 69, 120, 123, 189
FspBI CTAG 1 cut(s) 140
GluI GCNGC 5 cut(s) 48, 69, 120, 123, 189
GsaI CCCAGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 385
HphI GGTGA 1 cut(s) 351
Hpy166II GTNNAC 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 395
Hpy8I GTNNAC 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 337
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 65, 188
HpyF3I CTNAG 2 cut(s) 64, 183
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 7 cut(s) 25, 102, 133, 254, 277, 375, 408
Lsp1109I GCAGC 5 cut(s) 55, 59, 106, 109, 200
LweI GCATC 1 cut(s) 356
MaeI CTAG 1 cut(s) 140
MaeIII GTNAC 1 cut(s) 264
MboII GAAGA 1 cut(s) 245
MluCI AATT 2 cut(s) 77, 176
MnlI CCTC 2 cut(s) 120, 178
MseI TTAA 4 cut(s) 80, 414, 428, 433
MspA1I CMGCKG 1 cut(s) 68
MwoI GCNNNNNNNGC 3 cut(s) 56, 65, 188
NmuCI GTSAC 1 cut(s) 264
PkrI GCNGC 5 cut(s) 49, 70, 121, 124, 190
PspFI CCCAGC 1 cut(s) 11
PstNI CAGNNNCTG 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SaqAI TTAA 4 cut(s) 80, 414, 428, 433
SatI GCNGC 5 cut(s) 48, 69, 120, 123, 189
SetI ASST 7 cut(s) 21, 29, 70, 232, 370, 414, 421
SfaNI GCATC 1 cut(s) 356
SfcI CTRYAG 1 cut(s) 225
Sse9I AATT 2 cut(s) 77, 176
SspMI CTAG 1 cut(s) 140
TasI AATT 2 cut(s) 77, 176
Tru1I TTAA 4 cut(s) 80, 414, 428, 433
Tru9I TTAA 4 cut(s) 80, 414, 428, 433
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 5 cut(s) 47, 68, 119, 122, 188
Tsp45I GTSAC 1 cut(s) 264
TspRI CASTG 1 cut(s) 273
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.