Rh2AG159600

Transmembrane family 220, helix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013562
Physical Location & Seq
Forward (+)
15062469 .. 15065143
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG159600.1

Sequence Viewer

Length: 483 bp
ATGGCAACACCCAGCAAGCTATACACCTTGTGTTCTCTACTAATGGCAGCACTATTTGCTTACTCAGCTGCTGTTCAATTAAATGACCCTGATTGGTACTTTTGGTTTCCTCTTTATGCTGTTGCTTGTGTTGTAAATCTAGTGCACTGGGCTATCACATCCAAAAAAATAAACCAATTTGCTGACGCAGCACTATGGCTTGGAATGTTTCTATTTATCAAGGTTCTAGCTGAAGATTTTGTAAATGGTATATCTGGGTTTTGGTCACTGGATTTGAGTGAGAGAGTAATCAGGGAGAAGATAGGAAGTGGGTTAGTCATAATCTCTATGGTTTTGCAGTTGATGGCATCATTATCATCACCAGAAGCTCCCACACAGAAAAGGCCAAGATATCTTGAATATGGCATGGGAATCCTGGTGGGGTTTGGTTATGGACTCCCATTCGTCTTCTTTGTGGTCCTAAAAGGTGAAATGAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM220

Protein Analysis

160

Amino Acids

17.95

Weight (kDa)

7.73

Isoelectric Point (pI)

24.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM220 PF15071 18 - 114 2.3e-18 Transmembrane family 220, helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013562)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23755
fragaria_vesca FvH4_1g14340 FvH4_1g14340 FvH4_1g14340
malus_domestica MD15G1271500.v1.1
prunus_persica Prupe.7G146700_v2.0.a1 Prupe.7G146700_v2.0.a1
pyrus_communis pycom15g23550
rosa_chinensis RchiOBHm_Chr2g0103011
rosa_laevigata RLG00000017200
rosa_multiflora Rmu_sc0000388.1_g000018
rosa_roxburghii Rroxscaffold_2G00140000
rosa_rugosa Rorug02G0111400
rosa_samantha Rh2AG159600 Rh2BG166100 Rh2CG166300 Rh2DG165000
rosa_wichuraiana Rw2G012470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 30
AfaI GTAC 1 cut(s) 98
AgsI TTSAA 2 cut(s) 77, 398
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 4 cut(s) 19, 68, 230, 368
AluI AGCT 4 cut(s) 19, 68, 230, 368
Alw21I GWGCWC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 143
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 1 cut(s) 383
ApaLI GTGCAC 1 cut(s) 143
ApeKI GCWGC 3 cut(s) 47, 68, 188
AspS9I GGNCC 1 cut(s) 457
AsuHPI GGTGA 2 cut(s) 351, 479
AvaII GGWCC 1 cut(s) 457
BaeGI GKGCMC 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 3 cut(s) 55, 59, 200
BccI CCATC 1 cut(s) 337
BciT130I CCWGG 1 cut(s) 416
BfaI CTAG 2 cut(s) 140, 227
BisI GCNGC 3 cut(s) 48, 69, 189
BlsI GCNGC 3 cut(s) 49, 70, 190
Bme1390I CCNGG 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 457
BmgT120I GGNCC 1 cut(s) 457
BmrFI CCNGG 1 cut(s) 416
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 356
BmuI ACTGGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 439
Bse1I ACTGG 2 cut(s) 152, 273
BseBI CCWGG 1 cut(s) 416
BseGI GGATG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 2 cut(s) 152, 273
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 3 cut(s) 55, 59, 200
BseYI CCCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 147
BsnI GGCC 1 cut(s) 385
Bsp1286I GDGCHC 1 cut(s) 147
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 1 cut(s) 77
BsrI ACTGG 2 cut(s) 152, 273
Bst2UI CCWGG 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 64
BstF5I GGATG 1 cut(s) 158
BstMWI GCNNNNNNNGC 3 cut(s) 56, 65, 188
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 55, 59, 200
BstV2I GAAGAC 1 cut(s) 439
BsuRI GGCC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 158
BtsIMutI CAGTG 2 cut(s) 145, 266
Cac8I GCNNGC 1 cut(s) 17
CaiI CAGNNNCTG 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 457
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 97
CviAII CATG 1 cut(s) 406
CviJI RGCY 7 cut(s) 19, 68, 152, 199, 230, 368, 385
CviKI_1 RGCY 7 cut(s) 19, 68, 152, 199, 230, 368, 385
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 1 cut(s) 64
DraIII CACNNNGTG 1 cut(s) 30
Eco32I GATATC 1 cut(s) 392
Eco47I GGWCC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 414
EcoRV GATATC 1 cut(s) 392
FaeI CATG 1 cut(s) 409
FaiI YATR 9 cut(s) 22, 117, 196, 251, 320, 329, 402, 407, 432
FatI CATG 1 cut(s) 405
Fnu4HI GCNGC 3 cut(s) 48, 69, 189
FokI GGATG 1 cut(s) 145
Fsp4HI GCNGC 3 cut(s) 48, 69, 189
FspBI CTAG 2 cut(s) 140, 227
GluI GCNGC 3 cut(s) 48, 69, 189
GsaI CCCAGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 385
HgaI GACGC 1 cut(s) 194
Hin1II CATG 1 cut(s) 409
HinfI GANTC 2 cut(s) 411, 435
HphI GGTGA 2 cut(s) 351, 479
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 1 cut(s) 395
Hpy8I GTNNAC 1 cut(s) 145
HpyCH4V TGCA 2 cut(s) 145, 337
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 65, 188
HpyF3I CTNAG 1 cut(s) 64
Hsp92II CATG 1 cut(s) 409
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 9 cut(s) 25, 102, 133, 240, 254, 277, 375, 401, 428
Lsp1109I GCAGC 3 cut(s) 55, 59, 200
LweI GCATC 1 cut(s) 356
MaeI CTAG 2 cut(s) 140, 227
MaeIII GTNAC 1 cut(s) 264
MboII GAAGA 3 cut(s) 245, 310, 439
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 2 cut(s) 77, 176
MlyI GAGTC 1 cut(s) 429
MnlI CCTC 1 cut(s) 120
MseI TTAA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 3 cut(s) 56, 65, 188
NlaIII CATG 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 264
PfeI GAWTC 1 cut(s) 411
PkrI GCNGC 3 cut(s) 49, 70, 190
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 414
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 1 cut(s) 414
PspPI GGNCC 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SaqAI TTAA 1 cut(s) 80
SatI GCNGC 3 cut(s) 48, 69, 189
Sau96I GGNCC 1 cut(s) 457
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 147
SetI ASST 7 cut(s) 21, 29, 70, 225, 232, 370, 469
SfaNI GCATC 1 cut(s) 356
SinI GGWCC 1 cut(s) 457
Sse9I AATT 2 cut(s) 77, 176
SspMI CTAG 2 cut(s) 140, 227
StyD4I CCNGG 1 cut(s) 414
TasI AATT 2 cut(s) 77, 176
TfiI GAWTC 1 cut(s) 411
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TscAI CASTG 2 cut(s) 152, 273
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 3 cut(s) 47, 68, 188
Tsp45I GTSAC 1 cut(s) 264
TspRI CASTG 2 cut(s) 152, 273
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 457
XspI CTAG 2 cut(s) 140, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.