RLG00000017777

protein At4g37920, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013303
Physical Location & Seq
Reverse (-)
21079366 .. 21083451
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017777

Sequence Viewer

Length: 1287 bp
ATGAACAATCTGTTGGGACTTGAGCTTTCCACTTCCACAACCCACCACTCCATCTTCTTCAACACTTCAACCTTCAATGAAATCCTTTCCCCAACTACCCTTATCCCTCCCATATCCTCTGCCCCTCTCACTTCCAACCACAAAAGAAACACAAGAGCAACTCAATGCTTCAAAACCCACTCAAGCCCCACAATCAGTCCTCATGTTGAAGTGGAGGAGCAAGTAGAGGTTGAAGTTGCTGAGGGGTATACCATCACTCAATTTTGTGATAAGATAATTGATATTTTCTTGAATGAGAAGCCTCGGGTAAAGGAATGGAGGAGGTATCTGGTGTTTAGGGAGGAGTGGAAGAAATATAGAGATTGCTTTTATAATAGGTGTAGGAATCGGGCCGATGCGGAGAGTGATGTAACTATGAAGGAAAAATTGGTTTCTATGGGAAGAAAGGTGAAGAAGATTGATGATGAAATGGAAAGACATAGTGAACTTCTCAAGGAGATACAGGATAACCCAACTGACGTCAATGCAATAGTTGCACGGAGGCGTAAAGACTTCACAGAGGAATTCTTTCGACACCTGAACCTACTTTCCAAAGTTTATGATGGTTTGGAAGATCGTGATGCAATGGCTCGGCTTGGGGCTAGATGCTTGTCTGCTGTCAGTGCTTATGATAACACATTAGAATATGTGGACACATTAGATACAGCCCAGGCCAAATTCGATGATATCCTAAATTCACCTTCAATCGATGCAGCTTGTGAGAAAATTAAAAGCCTTGCCAAGGCAAAGGAGCTGGATTCTTCATTGGTATTATTGATAAACAGTGCTTGGGCTTCAGCAAAGGAGTCCACAACGATGAAAAATGAGGTCAAAGAAATAATGTATCGTTTGTACAAAGCCACAAAAAGCAGTCTTCGGAGCATTGTACCGAAAGAAATAAAGCTGCTCAAACATTTGCTGAACATAACAGATCCTGAAGAGCGATTCTCAACACTGGCAACAGCTTTCTGCCCTGGTGATGATAATGAAGCCAAGGACCCCAAAGCTGTGTACAGTACTCCCAAAGAGCTTCACAAGTGGATTAAGATCATGCTCGATGCATACCATCTAAACGCGGAAGAAACTGACCTTAGGGAAGCCAAGCAGATGACTCAGCCTGTGGTCATACAAAGGCTGTTCATCCTCAGGGAAACCATTGAAGAGGAATATTTGGAAAAAAGCACATCTCCGGAGTCTCAAACAGAGGAAACTAAATCAGAGGAGAATCAGGAGGATTTGGGGATTTAG

Protein Analysis

429

Amino Acids

49.31

Weight (kDa)

5.51

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37920
fragaria_vesca FvH4_1g18990 FvH4_1g18990 FvH4_1g18990 FvH4_1g18990 FvH4_1g18990
malus_domestica MD01G1044700.v1.1
prunus_persica Prupe.6G209500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0110011
rosa_laevigata RLG00000017777
rosa_multiflora Rmu_sc0001870.1_g000006
rosa_roxburghii Rroxscaffold_2G00133780
rosa_rugosa Rorug02G0165100
rosa_samantha Rh2AG216700 Rh2BG226700 Rh2CG218900 Rh2DG222300
rosa_wichuraiana Rw2G016750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AatII GACGTC 1 cut(s) 522
AccI GTMKAC 1 cut(s) 248
AccII CGCG 1 cut(s) 1115
AccIII TCCGGA 1 cut(s) 1228
AciI CCGC 2 cut(s) 398, 1115
AclWI GGATC 1 cut(s) 965
AcsI RAATTY 3 cut(s) 563, 716, 733
AcuI CTGAAG 2 cut(s) 819, 996
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 4 cut(s) 893, 927, 1052, 1057
AfiI CCNNNNNNNGG 1 cut(s) 781
AgsI TTSAA 9 cut(s) 61, 69, 76, 172, 209, 233, 292, 744, 1199
AjnI CCWGG 2 cut(s) 708, 1012
AjuI GAANNNNNNNTTGG 3 cut(s) 28, 410, 442
AloI GAACNNNNNNTCC 2 cut(s) 572, 604
AluBI AGCT 7 cut(s) 25, 755, 793, 943, 1004, 1046, 1069
AluI AGCT 7 cut(s) 25, 755, 793, 943, 1004, 1046, 1069
Alw26I GTCTC 1 cut(s) 1239
AlwI GGATC 1 cut(s) 965
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 1228
AoxI GGCC 2 cut(s) 390, 711
ApeKI GCWGC 2 cut(s) 752, 943
ApoI RAATTY 3 cut(s) 563, 716, 733
Asp700I GAANNNNTTC 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 390, 1036
AsuHPI GGTGA 3 cut(s) 460, 729, 1028
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 1 cut(s) 1036
AxyI CCTNAGG 2 cut(s) 1130, 1184
BbsI GAAGAC 1 cut(s) 905
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 764, 930
BccI CCATC 4 cut(s) 59, 260, 596, 1113
BciT130I CCWGG 2 cut(s) 710, 1014
BcoDI GTCTC 1 cut(s) 1239
BfaI CTAG 1 cut(s) 642
BisI GCNGC 2 cut(s) 753, 944
BlsI GCNGC 2 cut(s) 754, 945
BmcAI AGTACT 1 cut(s) 1057
Bme1390I CCNGG 2 cut(s) 710, 1014
Bme18I GGWCC 1 cut(s) 1036
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 2 cut(s) 390, 1036
BmiI GGNNCC 1 cut(s) 1038
BmrFI CCNGG 2 cut(s) 710, 1014
BmsI GCATC 5 cut(s) 385, 610, 635, 739, 1087
BpiI GAAGAC 1 cut(s) 905
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 3 cut(s) 41, 166, 476
Bsa29I ATCGAT 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 1085
BsaHI GRCGYC 1 cut(s) 519
BsaJI CCNNGG 5 cut(s) 302, 708, 780, 1012, 1032
BsaWI WCCGGW 1 cut(s) 1228
Bsc4I CCNNNNNNNGG 1 cut(s) 781
Bse1I ACTGG 1 cut(s) 999
Bse21I CCTNAGG 2 cut(s) 1130, 1184
Bse3DI GCAATG 1 cut(s) 630
Bse8I GATNNNNATC 1 cut(s) 1085
BseAI TCCGGA 1 cut(s) 1228
BseBI CCWGG 2 cut(s) 710, 1014
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 5 cut(s) 302, 708, 780, 1012, 1032
BseGI GGATG 1 cut(s) 1179
BseJI GATNNNNATC 1 cut(s) 1085
BseLI CCNNNNNNNGG 1 cut(s) 781
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 3 cut(s) 231, 1166, 1198
BseNI ACTGG 1 cut(s) 999
BseRI GAGGAG 4 cut(s) 230, 334, 356, 1274
BseXI GCAGC 2 cut(s) 764, 930
Bsh1236I CGCG 1 cut(s) 1115
BshFI GGCC 2 cut(s) 392, 713
BshVI ATCGAT 1 cut(s) 747
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 1 cut(s) 1229
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 781
BsmAI GTCTC 1 cut(s) 1239
BsmFI GGGAC 1 cut(s) 30
BsnI GGCC 2 cut(s) 392, 713
BsoBI CYCGRG 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 1228
Bsp1407I TGTACA 2 cut(s) 891, 1050
Bsp143I GATC 3 cut(s) 613, 970, 1086
BspACI CCGC 2 cut(s) 398, 1115
BspANI GGCC 2 cut(s) 392, 713
BspCNI CTCAG 3 cut(s) 232, 1165, 1197
BspDI ATCGAT 1 cut(s) 747
BspEI TCCGGA 1 cut(s) 1228
BspFNI CGCG 1 cut(s) 1115
BspLI GGNNCC 1 cut(s) 1038
BspPI GGATC 1 cut(s) 965
BspQI GCTCTTC 1 cut(s) 972
BsrDI GCAATG 1 cut(s) 630
BsrGI TGTACA 2 cut(s) 891, 1050
BsrI ACTGG 1 cut(s) 999
BssECI CCNNGG 5 cut(s) 302, 708, 780, 1012, 1032
BssMI GATC 3 cut(s) 613, 970, 1086
BssNAI GTATAC 1 cut(s) 249
BssNI GRCGYC 1 cut(s) 519
BssT1I CCWWGG 2 cut(s) 780, 1032
Bst1107I GTATAC 1 cut(s) 249
Bst2UI CCWGG 2 cut(s) 710, 1014
Bst4CI ACNGT 2 cut(s) 824, 1055
Bst6I CTCTTC 2 cut(s) 972, 1194
BstACI GRCGYC 1 cut(s) 519
BstAPI GCANNNNNTGC 1 cut(s) 533
BstAUI TGTACA 2 cut(s) 891, 1050
BstDEI CTNAG 4 cut(s) 240, 1130, 1152, 1184
BstENI CCTNNNNNAGG 1 cut(s) 779
BstF5I GGATG 1 cut(s) 1179
BstFNI CGCG 1 cut(s) 1115
BstKTI GATC 3 cut(s) 616, 973, 1089
BstMAI GTCTC 1 cut(s) 1239
BstMBI GATC 3 cut(s) 613, 970, 1086
BstMWI GCNNNNNNNGC 2 cut(s) 533, 662
BstNI CCWGG 2 cut(s) 710, 1014
BstSCI CCNGG 2 cut(s) 708, 1012
BstUI CGCG 1 cut(s) 1115
BstV1I GCAGC 2 cut(s) 764, 930
BstV2I GAAGAC 1 cut(s) 905
BstX2I RGATCY 1 cut(s) 970
BstYI RGATCY 1 cut(s) 970
BstZ17I GTATAC 1 cut(s) 249
Bsu15I ATCGAT 1 cut(s) 747
Bsu36I CCTNAGG 2 cut(s) 1130, 1184
BsuRI GGCC 2 cut(s) 392, 713
BsuTUI ATCGAT 1 cut(s) 747
BtsCI GGATG 1 cut(s) 1179
BtsIMutI CAGTG 3 cut(s) 667, 829, 992
CaiI CAGNNNCTG 1 cut(s) 974
Cfr13I GGNCC 2 cut(s) 390, 1036
ClaI ATCGAT 1 cut(s) 747
Csp6I GTAC 4 cut(s) 892, 926, 1051, 1056
CviAII CATG 2 cut(s) 203, 1090
CviQI GTAC 4 cut(s) 892, 926, 1051, 1056
DdeI CTNAG 4 cut(s) 240, 1130, 1152, 1184
DpnI GATC 3 cut(s) 615, 972, 1088
DpnII GATC 3 cut(s) 613, 970, 1086
Eam1104I CTCTTC 2 cut(s) 972, 1194
EarI CTCTTC 2 cut(s) 972, 1194
Eco130I CCWWGG 2 cut(s) 780, 1032
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 1036
Eco57I CTGAAG 2 cut(s) 819, 996
Eco81I CCTNAGG 2 cut(s) 1130, 1184
Eco88I CYCGRG 1 cut(s) 303
EcoNI CCTNNNNNAGG 1 cut(s) 779
EcoO109I RGGNCCY 1 cut(s) 1036
EcoRI GAATTC 1 cut(s) 563
EcoRII CCWGG 2 cut(s) 708, 1012
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 2 cut(s) 780, 1032
EcoT22I ATGCAT 1 cut(s) 1102
ErhI CCWWGG 2 cut(s) 780, 1032
FaeI CATG 2 cut(s) 206, 1093
FaqI GGGAC 1 cut(s) 30
FatI CATG 2 cut(s) 202, 1089
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 2 cut(s) 753, 944
FokI GGATG 1 cut(s) 1166
Fsp4HI GCNGC 2 cut(s) 753, 944
FspBI CTAG 1 cut(s) 642
GluI GCNGC 2 cut(s) 753, 944
HaeIII GGCC 2 cut(s) 392, 713
HapII CCGG 1 cut(s) 1229
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 2 cut(s) 206, 1093
HinfI GANTC 7 cut(s) 385, 797, 845, 984, 1150, 1232, 1264
HpaII CCGG 1 cut(s) 1229
HphI GGTGA 3 cut(s) 460, 729, 1028
Hpy166II GTNNAC 5 cut(s) 249, 485, 691, 849, 1051
Hpy188I TCNGA 2 cut(s) 918, 1258
Hpy188III TCNNGA 5 cut(s) 289, 617, 974, 1229, 1268
Hpy8I GTNNAC 5 cut(s) 249, 485, 691, 849, 1051
HpyAV CCTTC 3 cut(s) 82, 412, 750
HpyCH4III ACNGT 2 cut(s) 824, 1055
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 5 cut(s) 527, 536, 623, 752, 1100
HpyF10VI GCNNNNNNNGC 2 cut(s) 533, 662
HpyF3I CTNAG 4 cut(s) 240, 1130, 1152, 1184
HpySE526I ACGT 1 cut(s) 519
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 2 cut(s) 206, 1093
Kpn2I TCCGGA 1 cut(s) 1228
Kzo9I GATC 3 cut(s) 613, 970, 1086
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 3 cut(s) 217, 790, 918
Lsp1109I GCAGC 2 cut(s) 764, 930
LweI GCATC 5 cut(s) 385, 610, 635, 739, 1087
MaeI CTAG 1 cut(s) 642
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 1 cut(s) 409
MalI GATC 3 cut(s) 615, 972, 1088
MboI GATC 3 cut(s) 613, 970, 1086
MflI RGATCY 1 cut(s) 970
MluCI AATT 7 cut(s) 260, 276, 425, 563, 716, 733, 765
MlyI GAGTC 3 cut(s) 854, 1144, 1241
MmeI TCCRAC 1 cut(s) 159
Mph1103I ATGCAT 1 cut(s) 1102
MroI TCCGGA 1 cut(s) 1228
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 2 cut(s) 768, 1083
MslI CAYNNNNRTG 1 cut(s) 854
MspI CCGG 1 cut(s) 1229
MspR9I CCNGG 2 cut(s) 710, 1014
MvaI CCWGG 2 cut(s) 710, 1014
MvnI CGCG 1 cut(s) 1115
MwoI GCNNNNNNNGC 2 cut(s) 533, 662
NdeII GATC 3 cut(s) 613, 970, 1086
NlaIII CATG 2 cut(s) 206, 1093
NlaIV GGNNCC 1 cut(s) 1038
NmeAIII GCCGAG 1 cut(s) 610
NsiI ATGCAT 1 cut(s) 1102
PciSI GCTCTTC 1 cut(s) 972
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 4 cut(s) 385, 797, 984, 1264
PkrI GCNGC 2 cut(s) 754, 945
PleI GAGTC 3 cut(s) 853, 1144, 1240
PpsI GAGTC 3 cut(s) 853, 1144, 1240
PpuMI RGGWCCY 1 cut(s) 1036
PsiI TTATAA 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 1036
Psp6I CCWGG 2 cut(s) 708, 1012
PspGI CCWGG 2 cut(s) 708, 1012
PspN4I GGNNCC 1 cut(s) 1038
PspPI GGNCC 2 cut(s) 390, 1036
PspPPI RGGWCCY 1 cut(s) 1036
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 1 cut(s) 970
RsaI GTAC 4 cut(s) 893, 927, 1052, 1057
RsaNI GTAC 4 cut(s) 892, 926, 1051, 1056
RseI CAYNNNNRTG 1 cut(s) 854
SapI GCTCTTC 1 cut(s) 972
SaqAI TTAA 2 cut(s) 768, 1083
SatI GCNGC 2 cut(s) 753, 944
Sau3AI GATC 3 cut(s) 613, 970, 1086
Sau96I GGNCC 2 cut(s) 390, 1036
ScaI AGTACT 1 cut(s) 1057
SchI GAGTC 3 cut(s) 854, 1144, 1241
ScrFI CCNGG 2 cut(s) 710, 1014
SfaNI GCATC 5 cut(s) 385, 610, 635, 739, 1087
SinI GGWCC 1 cut(s) 1036
SmiMI CAYNNNNRTG 1 cut(s) 854
SmlI CTYRAG 3 cut(s) 20, 181, 491
SmoI CTYRAG 3 cut(s) 20, 181, 491
Sse9I AATT 7 cut(s) 260, 276, 425, 563, 716, 733, 765
SsiI CCGC 2 cut(s) 398, 1115
SspI AATATT 1 cut(s) 1208
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 708, 1012
StyI CCWWGG 2 cut(s) 780, 1032
TaaI ACNGT 2 cut(s) 824, 1055
TaiI ACGT 1 cut(s) 522
TaqI TCGA 4 cut(s) 571, 720, 747, 1095
TasI AATT 7 cut(s) 260, 276, 425, 563, 716, 733, 765
TatI WGTACW 3 cut(s) 891, 1050, 1055
TfiI GAWTC 4 cut(s) 385, 797, 984, 1264
Tru1I TTAA 2 cut(s) 768, 1083
Tru9I TTAA 2 cut(s) 768, 1083
TscAI CASTG 3 cut(s) 667, 829, 999
TseI GCWGC 2 cut(s) 752, 943
TspDTI ATGAA 8 cut(s) 17, 93, 431, 480, 792, 872, 1041, 1168
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 3 cut(s) 667, 829, 999
VpaK11BI GGWCC 1 cut(s) 1036
XagI CCTNNNNNAGG 1 cut(s) 779
XapI RAATTY 3 cut(s) 563, 716, 733
XmiI GTMKAC 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 1 cut(s) 642
ZraI GACGTC 1 cut(s) 520
ZrmI AGTACT 1 cut(s) 1057
Zsp2I ATGCAT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.