Rroxscaffold_2G00133780

protein At4g37920, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013303
Physical Location & Seq
Forward (+)
71283916 .. 71287195
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133780.1

Sequence Viewer

Length: 1314 bp
ATGAACAATCTGTTGGGACTTGAGCTTTCCACTTCCACAACCCACCATTCCATCTTCTTCAACACTTCAACCTCCTTTGACTTCTTCCCAACATTCAATGAAATCCTTTCCCCAACTACCCTTATCCCTCCCGTGTCCTCTGCCCCTCTCACTTCCAACCACAAAAGAAACACAAGAGCAACTCAATGCTTCAAAACCCATGCTGCCCACTCAAGCCCCACAATCAGTCCTCATGTTGAAGTGGAGGACCAAGTAGAGGTTGAAGTTGCTGAAGGGTACACCATCACTCAATTTTGTGATAAGATAATTGATATTTTCTTGAATGAGAAGCCTAGGGTAAAGGAATGGAGGAGGTATCTGGTGTTCAGGGAGGAGTGGAAGAAATATAGAGCTTGCTTTTATAATAGGTGTAGGAATCGGGCCGATGCGGAGAGTGATCCAACTATGAAAGAAAAATTGGTTTCCATGGGAAGAAAGGTGAAGAAGGAGTCAGCATTGCAGATTGATGATGAGATGGAAAGACATAGTGAACTTCTCAAGGAGATACAGGAGAACCCAACCGATGTCAATGCAATAGTTGCACGGAGGCGTAAAGACTTCACAGAGGAATTCTTTCGACACCTCAACCTACTTTCAGAAGTTTATGATGGTTTGGAGGATCGTGATGCAATGGCCAGGCTCGGGGCTAGATGCTTGTCCGCTGTCAGTGCTTATGATAACACACTAGAATATGTGGACACATTAGATACAGCCCAGGCCAAATTCGATGATATCTTAAATTCACCTTCAATCGATGTAGCATGTGAGAAAATTAAAAGCCTTGCCAAGGCAAAGGAACTGGATTCTTCATTGGTATTATTGATAAACAGTGCTTGGGCTTCTGCAAAGGAATCCACAACAATGAAAAATGAGGTCAAAGAAATAATGTATCGTTTGTACAAAGCCACAAAAAGCAGTCTTCGGAGCATTGTACCAAAAGAAATAAAGCTGCTCAAACATTTGCTGAACATAACAGATCCTGAAGAGCGATTCTCAGCACTGGCAACAGCTTTCTGTCCTGGTGATGGTACTGAAGCCAAGGACCCCAAAGCTGTGTACAGTACTCCCAAAGAGCTTCACAAGTGGATTAAGATCATGCTCGATGCATACCATCTAAACGCGGAAGAAACTGACCTTAGGGAAGCCAAGCAGATGACTCAGCCTGTGGTTATACAAAGGCTGTTCATCCTCAAGGAAACTATTGAAGAAGAATATTTGGAAAAAAGCACATCTCCGGAGTCTCAAACAGAGGAAACTAAATCAGAGGAGTTCTGA

Protein Analysis

437

Amino Acids

50.17

Weight (kDa)

5.57

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37920
fragaria_vesca FvH4_1g18990 FvH4_1g18990 FvH4_1g18990 FvH4_1g18990 FvH4_1g18990
malus_domestica MD01G1044700.v1.1
prunus_persica Prupe.6G209500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0110011
rosa_laevigata RLG00000017777
rosa_multiflora Rmu_sc0001870.1_g000006
rosa_roxburghii Rroxscaffold_2G00133780
rosa_rugosa Rorug02G0165100
rosa_samantha Rh2AG216700 Rh2BG226700 Rh2CG218900 Rh2DG222300
rosa_wichuraiana Rw2G016750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AccII CGCG 1 cut(s) 1160
AccIII TCCGGA 1 cut(s) 1273
AciI CCGC 3 cut(s) 428, 699, 1160
AclWI GGATC 3 cut(s) 431, 666, 1010
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 3 cut(s) 608, 761, 778
AcuI CTGAAG 3 cut(s) 291, 1041, 1092
AfaI GTAC 6 cut(s) 278, 938, 972, 1069, 1097, 1102
AfiI CCNNNNNNNGG 4 cut(s) 256, 681, 826, 1064
AgsI TTSAA 9 cut(s) 61, 69, 97, 193, 239, 263, 322, 789, 1244
AjnI CCWGG 3 cut(s) 674, 753, 1057
AjuI GAANNNNNNNTTGG 3 cut(s) 28, 440, 472
AluBI AGCT 6 cut(s) 25, 392, 988, 1049, 1091, 1114
AluI AGCT 6 cut(s) 25, 392, 988, 1049, 1091, 1114
Alw26I GTCTC 1 cut(s) 1284
AlwI GGATC 3 cut(s) 431, 666, 1010
AlwNI CAGNNNCTG 1 cut(s) 1019
Ama87I CYCGRG 1 cut(s) 680
Aor13HI TCCGGA 1 cut(s) 1273
AoxI GGCC 3 cut(s) 420, 672, 756
ApeKI GCWGC 2 cut(s) 203, 988
ApoI RAATTY 3 cut(s) 608, 761, 778
Asp700I GAANNNNTTC 1 cut(s) 612
AspA2I CCTAGG 1 cut(s) 332
AspS9I GGNCC 3 cut(s) 247, 420, 1081
AsuHPI GGTGA 3 cut(s) 490, 774, 1073
AvaI CYCGRG 1 cut(s) 680
AvaII GGWCC 2 cut(s) 247, 1081
AvrII CCTAGG 1 cut(s) 332
AxyI CCTNAGG 1 cut(s) 1175
BalI TGGCCA 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 950
BbvI GCAGC 2 cut(s) 190, 975
BccI CCATC 6 cut(s) 59, 290, 508, 641, 1058, 1158
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 3 cut(s) 676, 755, 1059
BcoDI GTCTC 1 cut(s) 1284
BfaI CTAG 3 cut(s) 333, 687, 725
BisI GCNGC 2 cut(s) 204, 989
BlnI CCTAGG 1 cut(s) 332
BlsI GCNGC 2 cut(s) 205, 990
BmcAI AGTACT 1 cut(s) 1102
Bme1390I CCNGG 3 cut(s) 676, 755, 1059
Bme18I GGWCC 2 cut(s) 247, 1081
BmeT110I CYCGRG 1 cut(s) 680
BmgT120I GGNCC 3 cut(s) 247, 420, 1081
BmiI GGNNCC 1 cut(s) 1083
BmrFI CCNGG 3 cut(s) 676, 755, 1059
BmsI GCATC 4 cut(s) 415, 655, 680, 1132
BpiI GAAGAC 1 cut(s) 950
BplI GAGNNNNNCTC 2 cut(s) 1016, 1048
BpuEI CTTGAG 4 cut(s) 41, 196, 521, 1214
Bsa29I ATCGAT 1 cut(s) 792
BsaBI GATNNNNATC 1 cut(s) 1130
BsaJI CCNNGG 5 cut(s) 332, 465, 753, 825, 1077
BsaWI WCCGGW 1 cut(s) 1273
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 681, 826, 1064
Bse1I ACTGG 2 cut(s) 843, 1044
Bse21I CCTNAGG 1 cut(s) 1175
Bse3DI GCAATG 2 cut(s) 494, 675
Bse8I GATNNNNATC 1 cut(s) 1130
BseAI TCCGGA 1 cut(s) 1273
BseBI CCWGG 3 cut(s) 676, 755, 1059
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 5 cut(s) 332, 465, 753, 825, 1077
BseGI GGATG 1 cut(s) 1224
BseJI GATNNNNATC 1 cut(s) 1130
BseLI CCNNNNNNNGG 4 cut(s) 256, 681, 826, 1064
BseMI GCAATG 2 cut(s) 494, 675
BseMII CTCAG 2 cut(s) 1047, 1211
BseNI ACTGG 2 cut(s) 843, 1044
BseRI GAGGAG 2 cut(s) 364, 386
BseXI GCAGC 2 cut(s) 190, 975
Bsh1236I CGCG 1 cut(s) 1160
BshFI GGCC 3 cut(s) 422, 674, 758
BshVI ATCGAT 1 cut(s) 792
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 1 cut(s) 1274
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 4 cut(s) 256, 681, 826, 1064
BsmAI GTCTC 1 cut(s) 1284
BsmFI GGGAC 1 cut(s) 30
BsnI GGCC 3 cut(s) 422, 674, 758
BsoBI CYCGRG 1 cut(s) 680
Bsp13I TCCGGA 1 cut(s) 1273
Bsp1407I TGTACA 2 cut(s) 936, 1095
Bsp143I GATC 4 cut(s) 436, 658, 1015, 1131
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 3 cut(s) 428, 699, 1160
BspANI GGCC 3 cut(s) 422, 674, 758
BspCNI CTCAG 2 cut(s) 1046, 1210
BspDI ATCGAT 1 cut(s) 792
BspEI TCCGGA 1 cut(s) 1273
BspFNI CGCG 1 cut(s) 1160
BspLI GGNNCC 1 cut(s) 1083
BspPI GGATC 3 cut(s) 431, 666, 1010
BspQI GCTCTTC 1 cut(s) 1017
BsrDI GCAATG 2 cut(s) 494, 675
BsrGI TGTACA 2 cut(s) 936, 1095
BsrI ACTGG 2 cut(s) 843, 1044
BssECI CCNNGG 5 cut(s) 332, 465, 753, 825, 1077
BssMI GATC 4 cut(s) 436, 658, 1015, 1131
BssT1I CCWWGG 4 cut(s) 332, 465, 825, 1077
Bst2UI CCWGG 3 cut(s) 676, 755, 1059
Bst4CI ACNGT 2 cut(s) 869, 1100
Bst6I CTCTTC 1 cut(s) 1017
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 2 cut(s) 936, 1095
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 3 cut(s) 1033, 1175, 1197
BstDSI CCRYGG 1 cut(s) 465
BstENI CCTNNNNNAGG 1 cut(s) 824
BstF5I GGATG 1 cut(s) 1224
BstFNI CGCG 1 cut(s) 1160
BstKTI GATC 4 cut(s) 439, 661, 1018, 1134
BstMAI GTCTC 1 cut(s) 1284
BstMBI GATC 4 cut(s) 436, 658, 1015, 1131
BstMWI GCNNNNNNNGC 2 cut(s) 578, 707
BstNI CCWGG 3 cut(s) 676, 755, 1059
BstNSI RCATGY 1 cut(s) 804
BstSCI CCNGG 3 cut(s) 674, 753, 1057
BstUI CGCG 1 cut(s) 1160
BstV1I GCAGC 2 cut(s) 190, 975
BstV2I GAAGAC 1 cut(s) 950
BstX2I RGATCY 1 cut(s) 1015
BstYI RGATCY 1 cut(s) 1015
Bsu15I ATCGAT 1 cut(s) 792
Bsu36I CCTNAGG 1 cut(s) 1175
BsuRI GGCC 3 cut(s) 422, 674, 758
BsuTUI ATCGAT 1 cut(s) 792
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 1 cut(s) 1224
BtsIMutI CAGTG 3 cut(s) 712, 874, 1037
Cac8I GCNNGC 1 cut(s) 394
CaiI CAGNNNCTG 1 cut(s) 1019
Cfr13I GGNCC 3 cut(s) 247, 420, 1081
ClaI ATCGAT 1 cut(s) 792
Csp6I GTAC 6 cut(s) 277, 937, 971, 1068, 1096, 1101
CviAII CATG 5 cut(s) 200, 233, 466, 801, 1135
CviQI GTAC 6 cut(s) 277, 937, 971, 1068, 1096, 1101
DdeI CTNAG 3 cut(s) 1033, 1175, 1197
DpnI GATC 4 cut(s) 438, 660, 1017, 1133
DpnII GATC 4 cut(s) 436, 658, 1015, 1131
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 1017
EarI CTCTTC 1 cut(s) 1017
Eco130I CCWWGG 4 cut(s) 332, 465, 825, 1077
Eco32I GATATC 1 cut(s) 772
Eco47I GGWCC 2 cut(s) 247, 1081
Eco57I CTGAAG 3 cut(s) 291, 1041, 1092
Eco81I CCTNAGG 1 cut(s) 1175
Eco88I CYCGRG 1 cut(s) 680
EcoNI CCTNNNNNAGG 1 cut(s) 824
EcoO109I RGGNCCY 1 cut(s) 1081
EcoRI GAATTC 1 cut(s) 608
EcoRII CCWGG 3 cut(s) 674, 753, 1057
EcoRV GATATC 1 cut(s) 772
EcoT14I CCWWGG 4 cut(s) 332, 465, 825, 1077
EcoT22I ATGCAT 1 cut(s) 1147
ErhI CCWWGG 4 cut(s) 332, 465, 825, 1077
FaeI CATG 5 cut(s) 203, 236, 469, 804, 1138
FaqI GGGAC 1 cut(s) 30
FatI CATG 5 cut(s) 199, 232, 465, 800, 1134
Fnu4HI GCNGC 2 cut(s) 204, 989
FokI GGATG 1 cut(s) 1211
Fsp4HI GCNGC 2 cut(s) 204, 989
FspBI CTAG 3 cut(s) 333, 687, 725
GluI GCNGC 2 cut(s) 204, 989
HaeIII GGCC 3 cut(s) 422, 674, 758
HapII CCGG 1 cut(s) 1274
Hin1II CATG 5 cut(s) 203, 236, 469, 804, 1138
HinfI GANTC 7 cut(s) 415, 488, 842, 890, 1029, 1195, 1277
HpaII CCGG 1 cut(s) 1274
HphI GGTGA 3 cut(s) 490, 774, 1073
Hpy166II GTNNAC 4 cut(s) 279, 530, 736, 1096
Hpy188I TCNGA 4 cut(s) 637, 963, 1303, 1313
Hpy188III TCNNGA 4 cut(s) 319, 662, 1019, 1274
Hpy8I GTNNAC 4 cut(s) 279, 530, 736, 1096
HpyAV CCTTC 3 cut(s) 266, 478, 795
HpyCH4III ACNGT 2 cut(s) 869, 1100
HpyCH4V TGCA 6 cut(s) 499, 572, 581, 668, 884, 1145
HpyF10VI GCNNNNNNNGC 2 cut(s) 578, 707
HpyF3I CTNAG 3 cut(s) 1033, 1175, 1197
Hsp92II CATG 5 cut(s) 203, 236, 469, 804, 1138
Kpn2I TCCGGA 1 cut(s) 1273
Kzo9I GATC 4 cut(s) 436, 658, 1015, 1131
LguI GCTCTTC 1 cut(s) 1017
LmnI GCTCC 1 cut(s) 963
Lsp1109I GCAGC 2 cut(s) 190, 975
LweI GCATC 4 cut(s) 415, 655, 680, 1132
MaeI CTAG 3 cut(s) 333, 687, 725
MalI GATC 4 cut(s) 438, 660, 1017, 1133
MboI GATC 4 cut(s) 436, 658, 1015, 1131
MflI RGATCY 1 cut(s) 1015
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 7 cut(s) 290, 306, 455, 608, 761, 778, 810
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 3 cut(s) 497, 1189, 1286
MmeI TCCRAC 2 cut(s) 180, 464
Mox20I TGGCCA 1 cut(s) 674
Mph1103I ATGCAT 1 cut(s) 1147
MroI TCCGGA 1 cut(s) 1273
MroXI GAANNNNTTC 1 cut(s) 612
MscI TGGCCA 1 cut(s) 674
MseI TTAA 3 cut(s) 776, 813, 1128
MslI CAYNNNNRTG 1 cut(s) 899
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 1 cut(s) 1274
MspR9I CCNGG 3 cut(s) 676, 755, 1059
MvaI CCWGG 3 cut(s) 676, 755, 1059
MvnI CGCG 1 cut(s) 1160
MwoI GCNNNNNNNGC 2 cut(s) 578, 707
NcoI CCATGG 1 cut(s) 465
NdeII GATC 4 cut(s) 436, 658, 1015, 1131
NlaIII CATG 5 cut(s) 203, 236, 469, 804, 1138
NlaIV GGNNCC 1 cut(s) 1083
NsiI ATGCAT 1 cut(s) 1147
NspI RCATGY 1 cut(s) 804
PciSI GCTCTTC 1 cut(s) 1017
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 4 cut(s) 415, 842, 890, 1029
PkrI GCNGC 2 cut(s) 205, 990
PleI GAGTC 3 cut(s) 496, 1189, 1285
PpsI GAGTC 3 cut(s) 496, 1189, 1285
PpuMI RGGWCCY 1 cut(s) 1081
PsiI TTATAA 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 1081
Psp6I CCWGG 3 cut(s) 674, 753, 1057
PspGI CCWGG 3 cut(s) 674, 753, 1057
PspN4I GGNNCC 1 cut(s) 1083
PspPI GGNCC 3 cut(s) 247, 420, 1081
PspPPI RGGWCCY 1 cut(s) 1081
PsrI GAACNNNNNNTAC 2 cut(s) 347, 379
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 1 cut(s) 1015
RsaI GTAC 6 cut(s) 278, 938, 972, 1069, 1097, 1102
RsaNI GTAC 6 cut(s) 277, 937, 971, 1068, 1096, 1101
RseI CAYNNNNRTG 1 cut(s) 899
SapI GCTCTTC 1 cut(s) 1017
SaqAI TTAA 3 cut(s) 776, 813, 1128
SatI GCNGC 2 cut(s) 204, 989
Sau3AI GATC 4 cut(s) 436, 658, 1015, 1131
Sau96I GGNCC 3 cut(s) 247, 420, 1081
ScaI AGTACT 1 cut(s) 1102
SchI GAGTC 3 cut(s) 497, 1189, 1286
ScrFI CCNGG 3 cut(s) 676, 755, 1059
SfaNI GCATC 4 cut(s) 415, 655, 680, 1132
SinI GGWCC 2 cut(s) 247, 1081
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 4 cut(s) 20, 211, 536, 1229
SmoI CTYRAG 4 cut(s) 20, 211, 536, 1229
Sse9I AATT 7 cut(s) 290, 306, 455, 608, 761, 778, 810
SsiI CCGC 3 cut(s) 428, 699, 1160
SspI AATATT 1 cut(s) 1253
SspMI CTAG 3 cut(s) 333, 687, 725
StyD4I CCNGG 3 cut(s) 674, 753, 1057
StyI CCWWGG 4 cut(s) 332, 465, 825, 1077
TaaI ACNGT 2 cut(s) 869, 1100
TaqI TCGA 4 cut(s) 616, 765, 792, 1140
TasI AATT 7 cut(s) 290, 306, 455, 608, 761, 778, 810
TatI WGTACW 3 cut(s) 936, 1095, 1100
TfiI GAWTC 4 cut(s) 415, 842, 890, 1029
Tru1I TTAA 3 cut(s) 776, 813, 1128
Tru9I TTAA 3 cut(s) 776, 813, 1128
TscAI CASTG 3 cut(s) 712, 874, 1044
TseI GCWGC 2 cut(s) 203, 988
TspDTI ATGAA 6 cut(s) 17, 114, 461, 837, 917, 1213
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 3 cut(s) 712, 874, 1044
VpaK11BI GGWCC 2 cut(s) 247, 1081
XagI CCTNNNNNAGG 1 cut(s) 824
XapI RAATTY 3 cut(s) 608, 761, 778
XceI RCATGY 1 cut(s) 804
XmaJI CCTAGG 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 3 cut(s) 333, 687, 725
ZrmI AGTACT 1 cut(s) 1102
Zsp2I ATGCAT 1 cut(s) 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.